Reviewed,
UniProtKB/Swiss-Prot P54572 (MAO1_BACSU)
Last modified
November 25, 2008.
Version 69.
History...
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Probable NAD-dependent malic enzyme 1 Short name=NAD-ME 1 EC=1.1.1.38 | ||||
| Gene names |
| ||||
| Organism | Bacillus subtilis [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 1423 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 439 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Catalytic activity | (S)-malate + NAD(+) = pyruvate + CO(2) + NADH. |
| Cofactor | Divalent metal cations. Prefers magnesium or manganese By similarity. |
| Sequence similarities | Belongs to the malic enzymes family. |
| Caution | Ref.4 has predicted this protein to be a malolactic enzyme which would convert malate to lactate. |
Ontologies
Keywords | |
|---|---|
| Ligand | Metal-binding NAD |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | NAD binding Inferred from electronic annotation. Source: InterPro amino acid bindingInferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activityInferred from electronic annotation. Source: EC metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 439 | 439 | Probable NAD-dependent malic enzyme 1 | PRO_0000160208 | |||||
Regions | |||||||||
| Nucleotide binding | 149 – 156 | 8 | NAD By similarity | ||||||
Sites | |||||||||
| Active site | 112 | 1 | Proton donor By similarity | ||||||
| Active site | 167 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 209 | 1 | Divalent metal cation By similarity | ||||||
| Metal binding | 210 | 1 | Divalent metal cation By similarity | ||||||
| Binding site | 235 | 1 | NAD By similarity | ||||||
| Binding site | 347 | 1 | NAD By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes." Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y. Microbiology 142:3103-3111(1996) [PubMed: 8969508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / JH642. |
| [2] | "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.  Danchin A.Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168. |
| [3] | "Physical and functional characterization of the Bacillus subtilis spoIIM gene." Smith K., Bayer M.E., Youngman P. J. Bacteriol. 175:3607-3617(1993) [PubMed: 8501064] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 432-439. |
| [4] | "Bacillus subtilis YqkI is a novel malic/Na+-lactate antiporter that enhances growth on malate at low protonmotive force." Wei Y., Guffanti A.A., Ito M., Krulwich T.A. J. Biol. Chem. 275:30287-30292(2000) [PubMed: 10903309] [Abstract] Cited for: DISCUSSION OF FUNCTION. |
Cross-references
Sequence databases | |
|---|---|
| D84432 Genomic DNA. Translation: BAA12645.1. Z99116 Genomic DNA. Translation: CAB14287.1. L06664 Genomic DNA. No translation available. | |
| PIR | C69967. |
| RefSeq | NP_390236.1. |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 938725. |
| GenomeReviews | Gene locus BSU23550 in contig AL009126_GR. |
| KEGG | bsu:BSU23550. |
| NMPDR | fig|224308.1.peg.2360. |
Organism-specific databases | |
| SubtiList | BG11764. yqkJ. [Micado] |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P54572. |
Enzyme and pathway databases | |
| BioCyc | BSUB224308:BSU2354-MON. |
Family and domain databases | |
| InterPro | IPR002912. ACT_bd. IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR016040. NAD(P)-bd. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| Pfam | PF01842. ACT. 1 hit. PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view] |
| PROSITE | PS00331. MALIC_ENZYMES. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MAO1_BACSU | ||||||||
| Accession | Primary (citable) accession number: P54572 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Bacillus subtilis Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList |
| SIMILARITY comments Index of protein domains and families |
