Reviewed, UniProtKB/Swiss-Prot P54552 (P5CR2_BACSU)

Names and origin

Protein names

Recommended name:
    Pyrroline-5-carboxylate reductase 2
      Short name=P5C reductase 2
      Short name=P5CR 2
    EC=1.5.1.2

Gene names

Name:	proI
Synonyms:	yqjO
Ordered Locus Names:	BSU23800

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-proline + NAD(P)(+) = 1-pyrroline-5-carboxylate + NAD(P)H.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the pyrroline-5-carboxylate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro pyrroline-5-carboxylate reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	Pyrroline-5-carboxylate reductase 2		PRO_0000187286

Sequences

	Sequence		Length	Mass (Da)
	P54552-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 4B69DB527E55345E	FASTA	278	30,396
	10 20 30 40 50 60 MKKIGFVGAG SMAEAMINGI LQSGITKPEH IYITNRSNDE RLIELKETYS VRPCRDKNEF 70 80 90 100 110 120 FTHTDIIILA FKPKDAAESI DSIRPYIKDQ LVISVLAGLT IETIQHYFGR KLAVIRVMPN 130 140 150 160 170 180 TSAAIRKSAT GFSVSTEASK NDIIAAKALL ETIGDATLVE ERHLDAVTAI AGSGPAYVYR 190 200 210 220 230 240 YIEAMEKAAQ KVGLDKETAK ALILQTMAGA TDMLLQSGKQ PEKLRKEITS PGGTTEAGLR 250 260 270 ALQDSRFEEA IIHCIEETAK RSAEIKEQFA GAALERHS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes." Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y. Microbiology 142:3103-3111(1996) [PubMed: 8969508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / JH642.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"Multiple genes for the last step of proline biosynthesis in Bacillus subtilis." Belitsky B.R., Brill J., Bremer E., Sonenshein A.L. J. Bacteriol. 183:4389-4392(2001) [PubMed: 11418582] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D84432 Genomic DNA. Translation: BAA12621.1. Z99116 Genomic DNA. Translation: CAB14312.1.
PIR	G69964.
RefSeq	NP_390261.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	938697.
GenomeReviews	Gene locus BSU23800 in contig AL009126_GR.
KEGG	bsu:BSU23800.
NMPDR	fig|224308.1.peg.2385.
Organism-specific databases
SubtiList	BG11744. proI. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P54552.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU2379-MON.
Family and domain databases
InterPro	IPR004455. NADP_OxRdtase_F420. IPR000304. P5CR. [Graphical view]
PANTHER	PTHR11645. P5CR. 1 hit.
Pfam	PF03807. F420_oxidored. 1 hit. [Graphical view]
PIRSF	PIRSF000193. Pyrrol-5-carb_rd. 1 hit.
TIGRFAMs	TIGR00112. proC. 1 hit.
PROSITE	PS00521. P5CR. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P54552.

Entry information

Entry name

P5CR2_BACSU

Accession

Primary (citable) accession number: P54552

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)