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Protein

NADPH dehydrogenase

Gene

namA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of the double bond of an array of alpha,beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes.1 Publication

Catalytic activityi

NADPH + acceptor = NADP+ + reduced acceptor.1 Publication

Cofactori

FMN1 Publication

Enzyme regulationi

Inhibited by p-hydroxybenzaldehyde (pHBA) and p-nitrophenol (pNP).

Kineticsi

The highest catalytic efficiency was observed with N-ethylmaleimide for which the KM is inferior to 1.0 µM.

  1. KM=19 µM for duroquinone1 Publication
  2. KM=109 µM for nitroglycerin1 Publication
  3. KM=293 µM for cyclohex-2-enone1 Publication
  4. KM=705 µM for 2,4,6-trinitrotoluene1 Publication
  5. KM=841 µM for menadione1 Publication
  6. KM=2602 µM for trans-hex-2-enal1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei28Substrate1 Publication1
    Binding sitei60FMN1 Publication1
    Binding sitei102FMN1 Publication1
    Binding sitei215FMN1 Publication1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi23 – 26FMN1 Publication4
    Nucleotide bindingi307 – 308FMN1 Publication2

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Detoxification, Stress response

    Keywords - Ligandi

    Flavoprotein, FMN, NADP

    Enzyme and pathway databases

    BioCyciBSUB:BSU23820-MONOMER.
    SABIO-RKP54550.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH dehydrogenase (EC:1.6.99.11 Publication)
    Alternative name(s):
    Xenobiotic reductase
    Gene namesi
    Name:namA
    Synonyms:yqjM
    Ordered Locus Names:BSU23820
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    Proteomesi
    • UP000001570 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved1 Publication
    ChainiPRO_00002161192 – 338NADPH dehydrogenaseAdd BLAST337

    Proteomic databases

    PaxDbiP54550.

    Expressioni

    Inductioni

    By toxic xenobiotic compounds (2,4,6-trinitrotoluene and nitroglycerin), and in response to oxidative stress (hydrogen peroxide and paraquat).1 Publication

    Interactioni

    Subunit structurei

    Homotetramer. Composed of a dimer of active dimers.2 Publications

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100013071.

    Structurei

    Secondary structure

    1338
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi4 – 6Combined sources3
    Beta strandi9 – 11Combined sources3
    Beta strandi14 – 22Combined sources9
    Beta strandi33 – 35Combined sources3
    Helixi39 – 50Combined sources12
    Beta strandi54 – 64Combined sources11
    Helixi65 – 67Combined sources3
    Beta strandi73 – 75Combined sources3
    Helixi79 – 81Combined sources3
    Helixi82 – 94Combined sources13
    Beta strandi98 – 104Combined sources7
    Helixi107 – 109Combined sources3
    Beta strandi117 – 121Combined sources5
    Helixi136 – 155Combined sources20
    Beta strandi159 – 165Combined sources7
    Helixi170 – 175Combined sources6
    Turni177 – 179Combined sources3
    Beta strandi187 – 189Combined sources3
    Helixi190 – 207Combined sources18
    Beta strandi212 – 217Combined sources6
    Helixi228 – 240Combined sources13
    Beta strandi245 – 249Combined sources5
    Turni262 – 265Combined sources4
    Helixi266 – 276Combined sources11
    Beta strandi279 – 282Combined sources4
    Helixi289 – 297Combined sources9
    Beta strandi302 – 306Combined sources5
    Helixi308 – 312Combined sources5
    Helixi316 – 323Combined sources8
    Helixi332 – 334Combined sources3
    Turni335 – 337Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1Z41X-ray1.30A/B1-338[»]
    1Z42X-ray1.85A/B1-338[»]
    1Z44X-ray1.40A/B1-338[»]
    1Z48X-ray1.80A/B1-338[»]
    ProteinModelPortaliP54550.
    SMRiP54550.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP54550.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni164 – 167Substrate binding1 Publication4

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105CCY. Bacteria.
    COG1902. LUCA.
    HOGENOMiHOG000116232.
    InParanoidiP54550.
    KOiK00354.
    OMAiGYQTRFA.
    PhylomeDBiP54550.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01614. NamA. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR023663. NADPH_DH.
    IPR001155. OxRdtase_FMN_N.
    [Graphical view]
    PfamiPF00724. Oxidored_FMN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P54550-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MARKLFTPIT IKDMTLKNRI VMSPMCMYSS HEKDGKLTPF HMAHYISRAI
    60 70 80 90 100
    GQVGLIIVEA SAVNPQGRIT DQDLGIWSDE HIEGFAKLTE QVKEQGSKIG
    110 120 130 140 150
    IQLAHAGRKA ELEGDIFAPS AIAFDEQSAT PVEMSAEKVK ETVQEFKQAA
    160 170 180 190 200
    ARAKEAGFDV IEIHAAHGYL IHEFLSPLSN HRTDEYGGSP ENRYRFLREI
    210 220 230 240 250
    IDEVKQVWDG PLFVRVSASD YTDKGLDIAD HIGFAKWMKE QGVDLIDCSS
    260 270 280 290 300
    GALVHADINV FPGYQVSFAE KIREQADMAT GAVGMITDGS MAEEILQNGR
    310 320 330
    ADLIFIGREL LRDPFFARTA AKQLNTEIPA PVQYERGW
    Length:338
    Mass (Da):37,584
    Last modified:January 23, 2007 - v3
    Checksum:iBDC52D34236326FE
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D84432 Genomic DNA. Translation: BAA12619.1.
    AL009126 Genomic DNA. Translation: CAB14314.1.
    PIRiE69964.
    RefSeqiNP_390263.1. NC_000964.3.
    WP_003230377.1. NZ_JNCM01000036.1.

    Genome annotation databases

    EnsemblBacteriaiCAB14314; CAB14314; BSU23820.
    GeneIDi938698.
    KEGGibsu:BSU23820.
    PATRICi18976589. VBIBacSub10457_2485.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D84432 Genomic DNA. Translation: BAA12619.1.
    AL009126 Genomic DNA. Translation: CAB14314.1.
    PIRiE69964.
    RefSeqiNP_390263.1. NC_000964.3.
    WP_003230377.1. NZ_JNCM01000036.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1Z41X-ray1.30A/B1-338[»]
    1Z42X-ray1.85A/B1-338[»]
    1Z44X-ray1.40A/B1-338[»]
    1Z48X-ray1.80A/B1-338[»]
    ProteinModelPortaliP54550.
    SMRiP54550.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100013071.

    Proteomic databases

    PaxDbiP54550.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAB14314; CAB14314; BSU23820.
    GeneIDi938698.
    KEGGibsu:BSU23820.
    PATRICi18976589. VBIBacSub10457_2485.

    Phylogenomic databases

    eggNOGiENOG4105CCY. Bacteria.
    COG1902. LUCA.
    HOGENOMiHOG000116232.
    InParanoidiP54550.
    KOiK00354.
    OMAiGYQTRFA.
    PhylomeDBiP54550.

    Enzyme and pathway databases

    BioCyciBSUB:BSU23820-MONOMER.
    SABIO-RKP54550.

    Miscellaneous databases

    EvolutionaryTraceiP54550.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01614. NamA. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR023663. NADPH_DH.
    IPR001155. OxRdtase_FMN_N.
    [Graphical view]
    PfamiPF00724. Oxidored_FMN. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiNAMA_BACSU
    AccessioniPrimary (citable) accession number: P54550
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: January 23, 2007
    Last modified: November 2, 2016
    This is version 115 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Forms a charge transfer complex with a variety of phenolic compounds.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.