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Protein

Ribonuclease Z

Gene

rnz

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.UniRule annotation1 Publication

Catalytic activityi

Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.UniRule annotation1 Publication

Cofactori

Zn2+2 PublicationsNote: Binds 2 Zn2+ ions.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi63Zinc 1; catalyticUniRule annotation1 Publication1
Metal bindingi65Zinc 1; catalyticUniRule annotation1 Publication1
Active sitei67Proton acceptorUniRule annotation1 Publication1
Metal bindingi67Zinc 2; catalyticUniRule annotation2 Publications1
Metal bindingi68Zinc 2; catalyticUniRule annotation2 Publications1
Metal bindingi140Zinc 1; catalyticUniRule annotation1 Publication1
Metal bindingi211Zinc 1; catalyticUniRule annotation1 Publication1
Metal bindingi211Zinc 2; catalyticUniRule annotation2 Publications1
Metal bindingi269Zinc 2; catalyticUniRule annotation2 Publications1

GO - Molecular functioni

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processtRNA processing
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU23840-MONOMER
BRENDAi3.1.26.11 658

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease ZUniRule annotation (EC:3.1.26.11UniRule annotation1 Publication)
Short name:
RNase ZUniRule annotation
Alternative name(s):
tRNA 3 endonucleaseUniRule annotation
tRNase ZUniRule annotation
Gene namesi
Name:rnzUniRule annotation
Synonyms:yqjK
Ordered Locus Names:BSU23840
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Pathology & Biotechi

Chemistry databases

DrugBankiDB03814 2-(N-Morpholino)-Ethanesulfonic Acid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001558491 – 307Ribonuclease ZAdd BLAST307

Proteomic databases

PaxDbiP54548
PRIDEiP54548

Interactioni

Subunit structurei

Homodimer.UniRule annotation2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-15572938,EBI-15572938

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-29070N
STRINGi224308.Bsubs1_010100013081

Structurei

Secondary structure

1307
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Beta strandi9 – 12Combined sources4
Beta strandi21 – 25Combined sources5
Turni27 – 30Combined sources4
Beta strandi31 – 36Combined sources6
Helixi42 – 46Combined sources5
Helixi53 – 55Combined sources3
Beta strandi56 – 60Combined sources5
Helixi66 – 68Combined sources3
Turni69 – 71Combined sources3
Helixi72 – 81Combined sources10
Beta strandi88 – 93Combined sources6
Helixi96 – 106Combined sources11
Beta strandi115 – 119Combined sources5
Beta strandi122 – 127Combined sources6
Beta strandi129 – 137Combined sources9
Beta strandi139 – 151Combined sources13
Helixi160 – 165Combined sources6
Helixi172 – 178Combined sources7
Beta strandi182 – 184Combined sources3
Beta strandi190 – 192Combined sources3
Helixi193 – 196Combined sources4
Beta strandi205 – 208Combined sources4
Helixi216 – 221Combined sources6
Turni222 – 224Combined sources3
Beta strandi226 – 231Combined sources6
Helixi239 – 244Combined sources6
Helixi250 – 260Combined sources11
Beta strandi263 – 268Combined sources6
Helixi278 – 289Combined sources12
Beta strandi291 – 295Combined sources5
Beta strandi301 – 303Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y44X-ray2.10A/B1-307[»]
2FK6X-ray2.90A1-307[»]
4GCWX-ray3.00A1-307[»]
ProteinModelPortaliP54548
SMRiP54548
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54548

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase Z family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105IES Bacteria
COG1234 LUCA
HOGENOMiHOG000272419
InParanoidiP54548
KOiK00784
OMAiGTQRQMM
PhylomeDBiP54548

Family and domain databases

CDDicd07717 RNaseZ_ZiPD-like_MBL-fold, 1 hit
Gene3Di3.60.15.10, 1 hit
HAMAPiMF_01818 RNase_Z_BN, 1 hit
InterProiView protein in InterPro
IPR001279 Metallo-B-lactamas
IPR036866 RibonucZ/Hydroxyglut_hydro
IPR013471 RNase_Z/BN
PfamiView protein in Pfam
PF12706 Lactamase_B_2, 2 hits
SMARTiView protein in SMART
SM00849 Lactamase_B, 1 hit
SUPFAMiSSF56281 SSF56281, 1 hit
TIGRFAMsiTIGR02651 RNase_Z, 1 hit

Sequencei

Sequence statusi: Complete.

P54548-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELLFLGTGA GIPAKARNVT SVALKLLEER RSVWLFDCGE ATQHQILHTT
60 70 80 90 100
IKPRKIEKIF ITHMHGDHVY GLPGLLGSRS FQGGEDELTV YGPKGIKAFI
110 120 130 140 150
ETSLAVTKTH LTYPLAIQEI EEGIVFEDDQ FIVTAVSVIH GVEAFGYRVQ
160 170 180 190 200
EKDVPGSLKA DVLKEMNIPP GPVYQKIKKG ETVTLEDGRI INGNDFLEPP
210 220 230 240 250
KKGRSVVFSG DTRVSDKLKE LARDCDVLVH EATFAKEDRK LAYDYYHSTT
260 270 280 290 300
EQAAVTAKEA RAKQLILTHI SARYQGDASL ELQKEAVDVF PNSVAAYDFL

EVNVPRG
Length:307
Mass (Da):34,023
Last modified:October 1, 1996 - v1
Checksum:i6E03CA54C55D7C84
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84432 Genomic DNA Translation: BAA12617.1
AL009126 Genomic DNA Translation: CAB14316.1
PIRiC69964
RefSeqiNP_390265.1, NC_000964.3
WP_004398681.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB14316; CAB14316; BSU23840
GeneIDi938694
KEGGibsu:BSU23840
PATRICifig|224308.179.peg.2597

Similar proteinsi

Entry informationi

Entry nameiRNZ_BACSU
AccessioniPrimary (citable) accession number: P54548
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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