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Protein

Glucose-6-phosphate 1-dehydrogenase

Gene

zwf

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.UniRule annotation

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.UniRule annotation

Pathwayi: pentose phosphate pathway

This protein is involved in step 1 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase (zwf)
  2. 6-phosphogluconolactonase (pgl)
  3. 6-phosphogluconate dehydrogenase, NADP(+)-dependent, decarboxylating (gndA)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei49NADPUniRule annotation1
Binding sitei149NADPUniRule annotation1
Binding sitei179SubstrateUniRule annotation1
Binding sitei183SubstrateUniRule annotation1
Binding sitei217SubstrateUniRule annotation1
Binding sitei236SubstrateUniRule annotation1
Active sitei241Proton acceptorUniRule annotation1
Binding sitei341SubstrateUniRule annotation1
Binding sitei346SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 22NADPUniRule annotation8
Nucleotide bindingi86 – 87NADPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciBSUB:BSU23850-MONOMER.
UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenaseUniRule annotation (EC:1.1.1.49UniRule annotation)
Short name:
G6PDUniRule annotation
Alternative name(s):
Vegetative protein 11
Short name:
VEG11
Gene namesi
Name:zwfUniRule annotation
Synonyms:yqjJ
Ordered Locus Names:BSU23850
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000681101 – 489Glucose-6-phosphate 1-dehydrogenaseAdd BLAST489

Proteomic databases

PaxDbiP54547.
PRIDEiP54547.

Interactioni

Protein-protein interaction databases

IntActiP54547. 1 interactor.
MINTiMINT-8366193.
STRINGi224308.Bsubs1_010100013086.

Structurei

3D structure databases

ProteinModelPortaliP54547.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glucose-6-phosphate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D8W. Bacteria.
COG0364. LUCA.
HOGENOMiHOG000046191.
InParanoidiP54547.
KOiK00036.
OMAiTIFEPIW.
PhylomeDBiP54547.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD. 1 hit.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54547-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTNQQPKAV IVIFGATGDL AKRKLYPSIH RLYQNGQIGE EFAVVGVGRR
60 70 80 90 100
PWSNEDLRQT VKTSISSSAD KHIDDFTSHF YYHPFDVTNP GSYQELNVLL
110 120 130 140 150
NQLEDTYQIP NNRMFYLAMA PEFFGTIAKT LKSEGVTATT GWSRLVIEKP
160 170 180 190 200
FGHDLPSAQA LNKEIREAFT EDQIYRIDHY LGKQMVQNIE VIRFANAIFE
210 220 230 240 250
PLWTNRYISN IQITSSESLG VEDRARYYEK SGALRDMVQN HIMQMVALLA
260 270 280 290 300
MEPPIKLNTE EIRSEKVKVL RALRPIAKDE VDEYFVRGQY HAGEIDGVPV
310 320 330 340 350
PAYTDEDNVA PDSNTETFVA GKLLIDNFRW AGVPFYIRTG KRMKEKSTKI
360 370 380 390 400
VVQFKDIPMN LYYGNENNMN PNLLVIHIQP DEGITLYLNA KKLGGAAHAQ
410 420 430 440 450
PIKLDYCSNC NDELNTPEAY EKLIHDCLLG DATNFAHWDE VALSWSFVDS
460 470 480
ISETWAANKT LSPNYESGSM GPKESDDLLV KDGLHWWNI
Length:489
Mass (Da):55,632
Last modified:July 7, 2009 - v2
Checksum:iB817901B5C0FF183
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti344K → R in BAA12616 (PubMed:8969508).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84432 Genomic DNA. Translation: BAA12616.1.
AL009126 Genomic DNA. Translation: CAB14317.2.
PIRiB69964.
RefSeqiNP_390266.2. NC_000964.3.
WP_003246151.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14317; CAB14317; BSU23850.
GeneIDi938690.
KEGGibsu:BSU23850.
PATRICi18976595. VBIBacSub10457_2488.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84432 Genomic DNA. Translation: BAA12616.1.
AL009126 Genomic DNA. Translation: CAB14317.2.
PIRiB69964.
RefSeqiNP_390266.2. NC_000964.3.
WP_003246151.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliP54547.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP54547. 1 interactor.
MINTiMINT-8366193.
STRINGi224308.Bsubs1_010100013086.

Proteomic databases

PaxDbiP54547.
PRIDEiP54547.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14317; CAB14317; BSU23850.
GeneIDi938690.
KEGGibsu:BSU23850.
PATRICi18976595. VBIBacSub10457_2488.

Phylogenomic databases

eggNOGiENOG4105D8W. Bacteria.
COG0364. LUCA.
HOGENOMiHOG000046191.
InParanoidiP54547.
KOiK00036.
OMAiTIFEPIW.
PhylomeDBiP54547.

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.
BioCyciBSUB:BSU23850-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD. 1 hit.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG6PD_BACSU
AccessioniPrimary (citable) accession number: P54547
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 7, 2009
Last modified: October 5, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.