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P54547 (G6PD_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphate 1-dehydrogenase

Short name=G6PD
EC=1.1.1.49
Alternative name(s):
Vegetative protein 11
Short name=VEG11
Gene names
Name:zwf
Synonyms:yqjJ
Ordered Locus Names:BSU23850
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length489 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone By similarity. HAMAP-Rule MF_00966

Catalytic activity

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH. HAMAP-Rule MF_00966

Pathway

Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. HAMAP-Rule MF_00966

Sequence similarities

Belongs to the glucose-6-phosphate dehydrogenase family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Glucose metabolism
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processpentose-phosphate shunt

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionNADP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glucose-6-phosphate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 489489Glucose-6-phosphate 1-dehydrogenase HAMAP-Rule MF_00966
PRO_0000068110

Regions

Nucleotide binding15 – 228NADP By similarity
Nucleotide binding86 – 872NADP By similarity

Sites

Active site2411Proton acceptor By similarity
Binding site491NADP By similarity
Binding site1491NADP By similarity
Binding site1791Substrate By similarity
Binding site1831Substrate By similarity
Binding site2171Substrate By similarity
Binding site2361Substrate By similarity
Binding site3411Substrate By similarity
Binding site3461Substrate By similarity

Experimental info

Sequence conflict3441K → R in BAA12616. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P54547 [UniParc].

Last modified July 7, 2009. Version 2.
Checksum: B817901B5C0FF183

FASTA48955,632
        10         20         30         40         50         60 
MKTNQQPKAV IVIFGATGDL AKRKLYPSIH RLYQNGQIGE EFAVVGVGRR PWSNEDLRQT 

        70         80         90        100        110        120 
VKTSISSSAD KHIDDFTSHF YYHPFDVTNP GSYQELNVLL NQLEDTYQIP NNRMFYLAMA 

       130        140        150        160        170        180 
PEFFGTIAKT LKSEGVTATT GWSRLVIEKP FGHDLPSAQA LNKEIREAFT EDQIYRIDHY 

       190        200        210        220        230        240 
LGKQMVQNIE VIRFANAIFE PLWTNRYISN IQITSSESLG VEDRARYYEK SGALRDMVQN 

       250        260        270        280        290        300 
HIMQMVALLA MEPPIKLNTE EIRSEKVKVL RALRPIAKDE VDEYFVRGQY HAGEIDGVPV 

       310        320        330        340        350        360 
PAYTDEDNVA PDSNTETFVA GKLLIDNFRW AGVPFYIRTG KRMKEKSTKI VVQFKDIPMN 

       370        380        390        400        410        420 
LYYGNENNMN PNLLVIHIQP DEGITLYLNA KKLGGAAHAQ PIKLDYCSNC NDELNTPEAY 

       430        440        450        460        470        480 
EKLIHDCLLG DATNFAHWDE VALSWSFVDS ISETWAANKT LSPNYESGSM GPKESDDLLV 


KDGLHWWNI 

« Hide

References

« Hide 'large scale' references
[1]"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / JH642.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 344.
[4]"First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis."
Antelmann H., Bernhardt J., Schmid R., Mach H., Voelker U., Hecker M.
Electrophoresis 18:1451-1463(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-15.
Strain: 168 / IS58.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D84432 Genomic DNA. Translation: BAA12616.1.
AL009126 Genomic DNA. Translation: CAB14317.2.
PIRB69964.
RefSeqNP_390266.2. NC_000964.3.

3D structure databases

ProteinModelPortalP54547.
SMRP54547. Positions 7-488.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP54547. 1 interaction.
MINTMINT-8366193.
STRING224308.BSU23850.

Proteomic databases

PaxDbP54547.
PRIDEP54547.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB14317; CAB14317; BSU23850.
GeneID938690.
KEGGbsu:BSU23850.
PATRIC18976595. VBIBacSub10457_2488.

Organism-specific databases

GenoListBSU23850. [Micado]

Phylogenomic databases

eggNOGCOG0364.
HOGENOMHOG000046191.
KOK00036.
OrthoDBEOG61308Z.
PhylomeDBP54547.

Enzyme and pathway databases

BioCycBSUB:BSU23850-MONOMER.
UniPathwayUPA00115; UER00408.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_00966. G6PD.
InterProIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23429. PTHR23429. 1 hit.
PfamPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFPIRSF000110. G6PD. 1 hit.
PRINTSPR00079. G6PDHDRGNASE.
TIGRFAMsTIGR00871. zwf. 1 hit.
PROSITEPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG6PD_BACSU
AccessionPrimary (citable) accession number: P54547
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 7, 2009
Last modified: July 9, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList