P54523 (DXS_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Synonyms:	yqiE
Ordered Locus Names:	BSU24270

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	633 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 633	633	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000189086

Sequences

	Sequence		Length	Mass (Da)
	P54523 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 333B65355F0CEF8F	FASTA	633	69,559
	10 20 30 40 50 60 MDLLSIQDPS FLKNMSIDEL EKLSDEIRQF LITSLSASGG HIGPNLGVVE LTVALHKEFN 70 80 90 100 110 120 SPKDKFLWDV GHQSYVHKLL TGRGKEFATL RQYKGLCGFP KRSESEHDVW ETGHSSTSLS 130 140 150 160 170 180 GAMGMAAARD IKGTDEYIIP IIGDGALTGG MALEALNHIG DEKKDMIVIL NDNEMSIAPN 190 200 210 220 230 240 VGAIHSMLGR LRTAGKYQWV KDELEYLFKK IPAVGGKLAA TAERVKDSLK YMLVSGMFFE 250 260 270 280 290 300 ELGFTYLGPV DGHSYHELIE NLQYAKKTKG PVLLHVITKK GKGYKPAETD TIGTWHGTGP 310 320 330 340 350 360 YKINTGDFVK PKAAAPSWSG LVSGTVQRMA REDGRIVAIT PAMPVGSKLE GFAKEFPDRM 370 380 390 400 410 420 FDVGIAEQHA ATMAAAMAMQ GMKPFLAIYS TFLQRAYDQV VHDICRQNAN VFIGIDRAGL 430 440 450 460 470 480 VGADGETHQG VFDIAFMRHI PNMVLMMPKD ENEGQHMVHT ALSYDEGPIA MRFPRGNGLG 490 500 510 520 530 540 VKMDEQLKTI PIGTWEVLRP GNDAVILTFG TTIEMAIEAA EELQKEGLSV RVVNARFIKP 550 560 570 580 590 600 IDEKMMKSIL KEGLPILTIE EAVLEGGFGS SILEFAHDQG EYHTPIDRMG IPDRFIEHGS 610 620 630 VTALLEEIGL TKQQVANRIR LLMPPKTHKG IGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes." Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y. Microbiology 142:3103-3111(1996) [PubMed: 8969508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / JH642.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D84432 Genomic DNA. Translation: BAA12576.1. AL009126 Genomic DNA. Translation: CAB14358.1.
PIR	B69961.
RefSeq	NP_390307.1. NC_000964.3.
3D structure databases
ProteinModelPortal	P54523.
SMR	P54523. Positions 2-624.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000001508; EBBACP00000001508; EBBACG00000001506.
GeneID	938609.
GenomeReviews	Gene locus BSU24270 in contig AL009126_GR.
KEGG	bsu:BSU24270.
NMPDR	fig|224308.1.peg.2431.
PATRIC	18976688. VBIBacSub10457_2534.
Organism-specific databases
GenoList	BSU24270. [Micado]
Phylogenomic databases
GeneTree	EBGT00070000031844.
HOGENOM	HBG571647.
OMA	QYDISYM.
PhylomeDB	P54523.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	BSUB:BSU24270-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. PF00456. Transketolase_N. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_BACSU

Accession

Primary (citable) accession number: P54523

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program