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Protein

3-dehydroquinate dehydratase

Gene

yqhS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Caution

Phe-23 is present instead of the conserved Tyr which is expected to be an active site residue.Curated

Catalytic activityi

3-dehydroquinate = 3-dehydroshikimate + H2O.

Pathwayi: chorismate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Protein AroA(G) (aroA)
  2. 3-dehydroquinate synthase (aroB), 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (yqhS), 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroF), Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei18Transition state stabilizerBy similarity1
Binding sitei74SubstrateBy similarity1
Binding sitei80SubstrateBy similarity1
Binding sitei87SubstrateBy similarity1
Active sitei100Proton donorBy similarity1
Binding sitei111SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU24470-MONOMER
UniPathwayiUPA00053; UER00086

Names & Taxonomyi

Protein namesi
Recommended name:
3-dehydroquinate dehydratase (EC:4.2.1.10)
Short name:
3-dehydroquinase
Alternative name(s):
Type II DHQase
Gene namesi
Name:yqhS
Ordered Locus Names:BSU24470
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001598721 – 1483-dehydroquinate dehydrataseAdd BLAST148

Proteomic databases

PaxDbiP54517
PRIDEiP54517

Interactioni

Subunit structurei

Homododecamer.1 Publication

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100013416

Structurei

Secondary structure

1148
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi12 – 14Combined sources3
Helixi20 – 23Combined sources4
Helixi28 – 42Combined sources15
Beta strandi45 – 50Combined sources6
Helixi54 – 64Combined sources11
Turni65 – 67Combined sources3
Beta strandi69 – 74Combined sources6
Helixi76 – 80Combined sources5
Helixi83 – 90Combined sources8
Beta strandi96 – 102Combined sources7
Helixi104 – 106Combined sources3
Helixi109 – 112Combined sources4
Helixi117 – 119Combined sources3
Beta strandi120 – 127Combined sources8
Helixi130 – 140Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GQOX-ray2.10A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/X/Y2-144[»]
ProteinModelPortaliP54517
SMRiP54517
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54517

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni101 – 102Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the type-II 3-dehydroquinase family.Curated

Phylogenomic databases

eggNOGiENOG4108Z38 Bacteria
COG0757 LUCA
HOGENOMiHOG000217278
InParanoidiP54517
KOiK03786
OMAiAYTHYSY
PhylomeDBiP54517

Family and domain databases

CDDicd00466 DHQase_II, 1 hit
Gene3Di3.40.50.9100, 1 hit
HAMAPiMF_00169 AroQ, 1 hit
InterProiView protein in InterPro
IPR001874 DHquinase_II
IPR018509 DHquinase_II_CS
IPR036441 DHquinase_II_sf
PANTHERiPTHR21272 PTHR21272, 1 hit
PfamiView protein in Pfam
PF01220 DHquinase_II, 1 hit
PIRSFiPIRSF001399 DHquinase_II, 1 hit
SUPFAMiSSF52304 SSF52304, 1 hit
TIGRFAMsiTIGR01088 aroQ, 1 hit
PROSITEiView protein in PROSITE
PS01029 DEHYDROQUINASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

P54517-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHFLILNGP NVNRLGSREP EVFGRQTLTD IETDLFQFAE ALHIQLTFFQ
60 70 80 90 100
SNHEGDLIDA IHEAEEQYSG IVLNPGALSH YSYAIRDAVS SISLPVVEVH
110 120 130 140
LSNLYAREEF RHQSVIAPVA KGQIVGLGAE GYKLAVRYLL SQQGGESR
Length:148
Mass (Da):16,431
Last modified:October 1, 1996 - v1
Checksum:i71B253931B43EFE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84432 Genomic DNA Translation: BAA12556.1
AL009126 Genomic DNA Translation: CAB14378.1
PIRiB69960
RefSeqiNP_390327.1, NC_000964.3
WP_003230223.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB14378; CAB14378; BSU24470
GeneIDi938557
KEGGibsu:BSU24470
PATRICifig|224308.179.peg.2665

Similar proteinsi

Entry informationi

Entry nameiAROQ_BACSU
AccessioniPrimary (citable) accession number: P54517
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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