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Protein

3-dehydroquinate dehydratase

Gene

yqhS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

3-dehydroquinate = 3-dehydroshikimate + H2O.

Pathwayi: chorismate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Protein AroA(G) (aroA)
  2. 3-dehydroquinate synthase (aroB), 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD), 3-dehydroquinate dehydratase (yqhS)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroF), Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei18Transition state stabilizerBy similarity1
Binding sitei74SubstrateBy similarity1
Binding sitei80SubstrateBy similarity1
Binding sitei87SubstrateBy similarity1
Active sitei100Proton donorBy similarity1
Binding sitei111SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU24470-MONOMER.
UniPathwayiUPA00053; UER00086.

Names & Taxonomyi

Protein namesi
Recommended name:
3-dehydroquinate dehydratase (EC:4.2.1.10)
Short name:
3-dehydroquinase
Alternative name(s):
Type II DHQase
Gene namesi
Name:yqhS
Ordered Locus Names:BSU24470
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001598721 – 1483-dehydroquinate dehydrataseAdd BLAST148

Proteomic databases

PaxDbiP54517.

Interactioni

Subunit structurei

Homododecamer.1 Publication

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100013416.

Structurei

Secondary structure

1148
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi12 – 14Combined sources3
Helixi20 – 23Combined sources4
Helixi28 – 42Combined sources15
Beta strandi45 – 50Combined sources6
Helixi54 – 64Combined sources11
Turni65 – 67Combined sources3
Beta strandi69 – 74Combined sources6
Helixi76 – 80Combined sources5
Helixi83 – 90Combined sources8
Beta strandi96 – 102Combined sources7
Helixi104 – 106Combined sources3
Helixi109 – 112Combined sources4
Helixi117 – 119Combined sources3
Beta strandi120 – 127Combined sources8
Helixi130 – 140Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GQOX-ray2.10A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/X/Y2-144[»]
ProteinModelPortaliP54517.
SMRiP54517.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54517.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni101 – 102Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the type-II 3-dehydroquinase family.Curated

Phylogenomic databases

eggNOGiENOG4108Z38. Bacteria.
COG0757. LUCA.
HOGENOMiHOG000217278.
InParanoidiP54517.
KOiK03786.
OMAiRREPGHY.
PhylomeDBiP54517.

Family and domain databases

CDDicd00466. DHQase_II. 1 hit.
Gene3Di3.40.50.9100. 1 hit.
HAMAPiMF_00169. AroQ. 1 hit.
InterProiIPR001874. DHquinase_II.
IPR018509. DHquinase_II_CS.
[Graphical view]
PANTHERiPTHR21272. PTHR21272. 1 hit.
PfamiPF01220. DHquinase_II. 1 hit.
[Graphical view]
PIRSFiPIRSF001399. DHquinase_II. 1 hit.
ProDomiPD004527. DHquinase_II. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52304. SSF52304. 1 hit.
TIGRFAMsiTIGR01088. aroQ. 1 hit.
PROSITEiPS01029. DEHYDROQUINASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54517-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHFLILNGP NVNRLGSREP EVFGRQTLTD IETDLFQFAE ALHIQLTFFQ
60 70 80 90 100
SNHEGDLIDA IHEAEEQYSG IVLNPGALSH YSYAIRDAVS SISLPVVEVH
110 120 130 140
LSNLYAREEF RHQSVIAPVA KGQIVGLGAE GYKLAVRYLL SQQGGESR
Length:148
Mass (Da):16,431
Last modified:October 1, 1996 - v1
Checksum:i71B253931B43EFE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84432 Genomic DNA. Translation: BAA12556.1.
AL009126 Genomic DNA. Translation: CAB14378.1.
PIRiB69960.
RefSeqiNP_390327.1. NC_000964.3.
WP_003230223.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14378; CAB14378; BSU24470.
GeneIDi938557.
KEGGibsu:BSU24470.
PATRICi18976728. VBIBacSub10457_2554.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84432 Genomic DNA. Translation: BAA12556.1.
AL009126 Genomic DNA. Translation: CAB14378.1.
PIRiB69960.
RefSeqiNP_390327.1. NC_000964.3.
WP_003230223.1. NZ_JNCM01000036.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GQOX-ray2.10A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/X/Y2-144[»]
ProteinModelPortaliP54517.
SMRiP54517.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100013416.

Proteomic databases

PaxDbiP54517.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14378; CAB14378; BSU24470.
GeneIDi938557.
KEGGibsu:BSU24470.
PATRICi18976728. VBIBacSub10457_2554.

Phylogenomic databases

eggNOGiENOG4108Z38. Bacteria.
COG0757. LUCA.
HOGENOMiHOG000217278.
InParanoidiP54517.
KOiK03786.
OMAiRREPGHY.
PhylomeDBiP54517.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00086.
BioCyciBSUB:BSU24470-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP54517.

Family and domain databases

CDDicd00466. DHQase_II. 1 hit.
Gene3Di3.40.50.9100. 1 hit.
HAMAPiMF_00169. AroQ. 1 hit.
InterProiIPR001874. DHquinase_II.
IPR018509. DHquinase_II_CS.
[Graphical view]
PANTHERiPTHR21272. PTHR21272. 1 hit.
PfamiPF01220. DHquinase_II. 1 hit.
[Graphical view]
PIRSFiPIRSF001399. DHquinase_II. 1 hit.
ProDomiPD004527. DHquinase_II. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52304. SSF52304. 1 hit.
TIGRFAMsiTIGR01088. aroQ. 1 hit.
PROSITEiPS01029. DEHYDROQUINASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAROQ_BACSU
AccessioniPrimary (citable) accession number: P54517
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Phe-23 is present instead of the conserved Tyr which is expected to be an active site residue.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.