P54512 (MNTR_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional regulator MntR

Alternative name(s):
Manganese transport regulator

Gene names

Name:	mntR
Synonyms:	yqhN
Ordered Locus Names:	BSU24520

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	142 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Central regulator of manganese homeostasis. In the presence of manganese, it mediates repression of the manganese transporter MntH; under low manganese conditions, it activates the transcription of the mntABCD operon. HAMAP-Rule MF_00732
Subunit structure	Homodimer Probable.
Subcellular location	Cytoplasm Probable HAMAP-Rule MF_00732.
Domain	It contains an N-terminal DNA-binding domain and a metal-binding domain. HAMAP-Rule MF_00732
Sequence similarities	Contains 1 HTH dtxR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Ligand	DNA-binding Manganese
Molecular function	Activator Repressor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular manganese ion homeostasis Inferred from electronic annotation. Source: HAMAP positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 142

142

Transcriptional regulator MntR HAMAP-Rule MF_00732

PRO_0000201118

Regions

Domain

1 – 63

63

HTH dtxR-type

Experimental info

Sequence conflict

81

1

E → D in BAA12551. Ref.1

Secondary structure

1

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142

Helix Strand Turn

Details...

Helix

4 – 20

17

Helix

25 – 32

8

Helix

36 – 48

13

Beta strand

51 – 55

5

Turn

56 – 58

3

Beta strand

59 – 62

4

Helix

64 – 86

23

Helix

91 – 101

11

Helix

102 – 104

3

Helix

107 – 121

15

Helix

124 – 134

11

Helix

139 – 141

3

Sequences

	Sequence		Length	Mass (Da)
	P54512 [UniParc]. Last modified July 7, 2009. Version 2. Checksum: 653BD8E0F4AB9F2C	FASTA	142	16,759
	10 20 30 40 50 60 MTTPSMEDYI EQIYMLIEEK GYARVSDIAE ALAVHPSSVT KMVQKLDKDE YLIYEKYRGL 70 80 90 100 110 120 VLTSKGKKIG KRLVYRHELL EQFLRIIGVD EEKIYNDVEG IEHHLSWNSI DRIGDLVQYF 130 140 EEDDARKKDL KSIQKKTEHH NQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes." Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y. Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / JH642.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 81.
[4]	"Manganese homeostasis in Bacillus subtilis is regulated by MntR, a bifunctional regulator related to the diphtheria toxin repressor family of proteins." Que Q., Helmann J.D. Mol. Microbiol. 35:1454-1468(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: 168.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D84432 Genomic DNA. Translation: BAA12551.1.
AL009126 Genomic DNA. Translation: CAB14383.2.

PIR

E69959.

RefSeq

NP_390332.2. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ON1	X-ray	1.75	A/B	1-142	[»]
1ON2	X-ray	1.61	A/B	1-142	[»]
2EV0	X-ray	1.65	A/B	1-142	[»]
2EV5	X-ray	2.00	A/B	1-142	[»]
2EV6	X-ray	1.70	A/B	1-142	[»]
2F5C	X-ray	2.40	A	1-142	[»]
2F5D	X-ray	1.90	A/B	1-142	[»]
2F5E	X-ray	2.20	A/B	1-142	[»]
2F5F	X-ray	2.40	A/B	1-142	[»]
2HYF	X-ray	2.80	A/B/C/D	1-142	[»]
2HYG	X-ray	2.80	D	1-142	[»]
3R60	X-ray	1.80	A/B	2-142	[»]
3R61	X-ray	1.90	A/B	2-142	[»]
4HV5	X-ray	1.90	A/B	2-142	[»]
4HV6	X-ray	2.30	A/B	2-142	[»]
4HX4	X-ray	1.65	A/B	2-142	[»]
4HX7	X-ray	1.90	A/B	2-142	[»]
4HX8	X-ray	2.00	A/B	2-142	[»]

ProteinModelPortal

P54512.

SMR

P54512. Positions 4-140.

ModBase

Search...

Protein-protein interaction databases

STRING

224308.BSU24520.

Proteomic databases

PaxDb

P54512.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CAB14383; CAB14383; BSU24520.

GeneID

938554.

KEGG

bsu:BSU24520.

PATRIC

18976738. VBIBacSub10457_2559.

Organism-specific databases

GenoList

BSU24520. [Micado]

Phylogenomic databases

eggNOG

COG1321.

HOGENOM

HOG000040349.

ProtClustDB

PRK03902.

Enzyme and pathway databases

BioCyc

BSUB:BSU24520-MONOMER.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.
1.10.60.10. 1 hit.

HAMAP

MF_00732. HTH_MntR.

InterPro

IPR001367. Fe_dep_repressor.
IPR022689. Fe_dep_repressor_HTH_DtxR.
IPR022687. Fe_dep_repressor_HTH_DtxR_N.
IPR022897. Tscrpt_reg_MntR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF02742. Fe_dep_repr_C. 1 hit.
PF01325. Fe_dep_repress. 1 hit.
[Graphical view]

SMART

SM00529. HTH_DTXR. 1 hit.
[Graphical view]

SUPFAM

SSF47979. HTH_DtxR. 1 hit.

PROSITE

PS50944. HTH_DTXR. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P54512.

Entry information

Entry name

MNTR_BACSU

Accession

Primary (citable) accession number: P54512

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	July 7, 2009
Last modified:	May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program