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P54475 (CSHB_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DEAD-box ATP-dependent RNA helicase CshB
EC=3.6.4.13
Gene names
Name:cshB
Synonyms:yqfR
Ordered Locus Names:BSU25140
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length438 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May work in conjunction with the cold shock proteins to ensure proper initiation of transcription at low and optimal temperatures. Ref.4

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Interacts with CspB. Ref.4

Subcellular location

Cytoplasm › nucleoid. Note: Shows transcription-dependent localization at subcellular sites surrounding the nucleoid. Ref.4

Induction

Induced by cold shock. Ref.3 Ref.4

Miscellaneous

The presence of CshA or CshB is essential for viability.

Sequence similarities

Belongs to the DEAD box helicase family.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Ontologies

Keywords
   Biological processStress response
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
RNA-binding
   Molecular functionHelicase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processresponse to cold

Inferred from genetic interaction Ref.4. Source: UniProtKB

   Cellular componentbacterial nucleoid

Inferred from direct assay Ref.4. Source: UniProtKB

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionATP binding

Inferred from sequence or structural similarity. Source: UniProtKB

ATP-dependent RNA helicase activity

Inferred from sequence or structural similarity. Source: UniProtKB

RNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 438438DEAD-box ATP-dependent RNA helicase CshB
PRO_0000055111

Regions

Domain35 – 208174Helicase ATP-binding
Domain235 – 385151Helicase C-terminal
Nucleotide binding48 – 558ATP By similarity
Motif4 – 3229Q motif
Motif156 – 1594DEAD box

Sequences

Sequence LengthMass (Da)Tools
P54475 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: B6D425EF6D433789

FASTA43850,027
        10         20         30         40         50         60 
MKETKFELYE LKPFIIDAVH RLGFYEPTDI QKRLIPAVLK KESVIGQSQT GTGKTHAYLL 

        70         80         90        100        110        120 
PLLNKIDPAK DVVQVVITAP TRELANQIYQ EALKITQGEE GSQIRSKCFI GGTDKQKSID 

       130        140        150        160        170        180 
KLKIQPHLVV GTPGRIADLI KEQALSVHKA ESLVIDEADL MLDMGFLADV DYIGSRMPED 

       190        200        210        220        230        240 
LQMLVFSATI PEKLKPFLKK YMENPKYAHV EPKQVTAAKI EHILIPSKHR DKDKLLFDIM 

       250        260        270        280        290        300 
SHLNPYLGIV FANTKNTADH IAQYLTGKGM KIGLLHGGLT PRERKKVMKQ INDLEFTYII 

       310        320        330        340        350        360 
ATDLAARGID IKGVSHVINY ELPDDLDFYV HRVGRTARAG SSGQAMTIYE LTDEDALVRL 

       370        380        390        400        410        420 
EKMGIEFEYL ELEKGEWKKG DDRQRRKKRK KTPNEADEIA HRLVKKPKKV KPGYKKKMSY 

       430 
EMEKIKKKQR RNQSKKRK

« Hide

References

« Hide 'large scale' references
[1]"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
Microbiology 142:3103-3111(1996) [PubMed: 8969508] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / JH642.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Genomewide transcriptional analysis of the cold shock response in Bacillus subtilis."
Beckering C.L., Steil L., Weber M.H.W., Voelker U., Marahiel M.A.
J. Bacteriol. 184:6395-6402(2002) [PubMed: 12399512] [Abstract]
Cited for: INDUCTION BY COLD SHOCK.
Strain: 168 / JH642.
[4]"Cold-induced putative DEAD box RNA helicases CshA and CshB are essential for cold adaptation and interact with cold shock protein B in Bacillus subtilis."
Hunger K., Beckering C.L., Wiegeshoff F., Graumann P.L., Marahiel M.A.
J. Bacteriol. 188:240-248(2006) [PubMed: 16352840] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CSPB, INDUCTION.
Strain: 168 / JH642.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D84432 Genomic DNA. Translation: BAA12495.1.
AL009126 Genomic DNA. Translation: CAB14444.1.
PIRD69954.
RefSeqNP_390393.1. NC_000964.3.

3D structure databases

ProteinModelPortalP54475.
SMRP54475. Positions 3-369.
ModBaseSearch...

Protein-protein interaction databases

IntActP54475. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000001897; EBBACP00000001897; EBBACG00000001894.
GeneID937908.
GenomeReviewsGene locus BSU25140 in contig AL009126_GR.
KEGGbsu:BSU25140.
NMPDRfig|224308.1.peg.2518.
PATRIC18976864. VBIBacSub10457_2621.

Organism-specific databases

GenoListBSU25140. [Micado]

Phylogenomic databases

GeneTreeEBGT00070000031730.
HOGENOMHBG737336.
OMAIEHVLIP.
PhylomeDBP54475.
ProtClustDBCLSK2301471.

Enzyme and pathway databases

BioCycBSUB:BSU25140-MONOMER.

Family and domain databases

InterProIPR014001. DEAD-like_helicase.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR001650. Helicase_C.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
PROSITEPS00039. DEAD_ATP_HELICASE. False negative.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCSHB_BACSU
AccessionPrimary (citable) accession number: P54475
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 25, 2012
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents