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Protein

tRNA (adenine(22)-N(1))-methyltransferase

Gene

trmK

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the S-adenosyl-L-methionine-dependent formation of N(1)-methyladenine at position 22 (m1A22) in tRNA.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + adenine(22) in tRNA = S-adenosyl-L-homocysteine + N(1)-methyladenine(22) in tRNA.1 Publication

GO - Molecular functioni

  • tRNA (adenine-N1-)-methyltransferase activity Source: UniProtKB

GO - Biological processi

  • tRNA methylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBSUB:BSU25180-MONOMER.
MetaCyc:BSU25180-MONOMER.
BRENDAi2.1.1.217. 658.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (adenine(22)-N(1))-methyltransferase (EC:2.1.1.217)
Alternative name(s):
TrMet(m1A22)
tRNA(m1A22)-methyltransferase
Short name:
tRNA(m1A22)MTase
Gene namesi
Name:trmK
Synonyms:yqfN
Ordered Locus Names:BSU25180
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000497981 – 238tRNA (adenine(22)-N(1))-methyltransferaseAdd BLAST238

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei70Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP54471.
PRIDEiP54471.

PTM databases

iPTMnetiP54471.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100013776.

Family & Domainsi

Sequence similaritiesi

Belongs to the TrmK family.Curated

Phylogenomic databases

eggNOGiCOG2384. LUCA.
HOGENOMiHOG000014372.
InParanoidiP54471.
KOiK06967.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR006901. TrmK.
[Graphical view]
PfamiPF04816. TrmK. 1 hit.
[Graphical view]
PIRSFiPIRSF018637. TrmK. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

P54471-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNELKLSKRL QTVAEYIPNG AVMADIGSDH AYLPCYAVLN HKASGAIAGE
60 70 80 90 100
ITDGPFLSAK RQVEKSGLNS HISVRQGDGL EVIKKGEADA ITIAGMGGAL
110 120 130 140 150
IAHILEAGKD KLTGKERLIL QPNIHAVHIR EWLYKERYAL IDEVILEEDG
160 170 180 190 200
KCYEVLVAEA GDRDAAYDGI SLSAGMLVGP FLAKEKNAVF LKKWTQELQH
210 220 230
TQSIYEQISQ AADTEQNKQK LKELADRMEL LKEVIDHG
Length:238
Mass (Da):26,162
Last modified:May 3, 2011 - v2
Checksum:iF59DA3FB5091C0C9
GO

Sequence cautioni

The sequence BAA12491 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAB14448 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84432 Genomic DNA. Translation: BAA12491.1. Different initiation.
AL009126 Genomic DNA. Translation: CAB14448.1. Different initiation.
PIRiH69953.
RefSeqiNP_390397.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB14448; CAB14448; BSU25180.
GeneIDi937898.
KEGGibsu:BSU25180.
PATRICi18976872. VBIBacSub10457_2625.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84432 Genomic DNA. Translation: BAA12491.1. Different initiation.
AL009126 Genomic DNA. Translation: CAB14448.1. Different initiation.
PIRiH69953.
RefSeqiNP_390397.1. NC_000964.3.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100013776.

PTM databases

iPTMnetiP54471.

Proteomic databases

PaxDbiP54471.
PRIDEiP54471.

Protocols and materials databases

DNASUi937898.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14448; CAB14448; BSU25180.
GeneIDi937898.
KEGGibsu:BSU25180.
PATRICi18976872. VBIBacSub10457_2625.

Phylogenomic databases

eggNOGiCOG2384. LUCA.
HOGENOMiHOG000014372.
InParanoidiP54471.
KOiK06967.

Enzyme and pathway databases

BioCyciBSUB:BSU25180-MONOMER.
MetaCyc:BSU25180-MONOMER.
BRENDAi2.1.1.217. 658.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR006901. TrmK.
[Graphical view]
PfamiPF04816. TrmK. 1 hit.
[Graphical view]
PIRSFiPIRSF018637. TrmK. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMK_BACSU
AccessioniPrimary (citable) accession number: P54471
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 3, 2011
Last modified: October 5, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.