Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Threonylcarbamoyladenosine tRNA methylthiotransferase MtaB

Gene

mtaB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t6A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms2t6A) at position 37 in tRNAs that read codons beginning with adenine.2 Publications

Catalytic activityi

N(6)-L-threonylcarbamoyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N(6)-L-threonylcarbamoyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.1 Publication

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi11 – 111Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi47 – 471Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi78 – 781Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi153 – 1531Iron-sulfur (4Fe-4S-S-AdoMet)PROSITE-ProRule annotation
Metal bindingi157 – 1571Iron-sulfur (4Fe-4S-S-AdoMet)PROSITE-ProRule annotation
Metal bindingi160 – 1601Iron-sulfur (4Fe-4S-S-AdoMet)PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • tRNA methylthiolation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBSUB:BSU25430-MONOMER.
BRENDAi2.8.4.5. 658.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonylcarbamoyladenosine tRNA methylthiotransferase MtaB (EC:2.8.4.51 Publication)
Alternative name(s):
tRNA-t(6)A37 methylthiotransferase
Gene namesi
Name:mtaB
Synonyms:tmtB, yqeV
Ordered Locus Names:BSU25430
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 451451Threonylcarbamoyladenosine tRNA methylthiotransferase MtaBPRO_0000141729Add
BLAST

Proteomic databases

PaxDbiP54462.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100013906.

Structurei

3D structure databases

ProteinModelPortaliP54462.
SMRiP54462. Positions 141-436.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 114113MTTase N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini372 – 43766TRAMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 MTTase N-terminal domain.PROSITE-ProRule annotation
Contains 1 TRAM domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105CIW. Bacteria.
COG0621. LUCA.
HOGENOMiHOG000224765.
InParanoidiP54462.
KOiK18707.
OMAiDCSYCIT.
PhylomeDBiP54462.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006467. MiaB-like_C.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01579. MiaB-like-C. 1 hit.
TIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54462-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATVAFHTLG CKVNHYETEA IWQLFKEAGY ERRDFEQTAD VYVINTCTVT
60 70 80 90 100
NTGDKKSRQV IRRAIRQNPD GVICVTGCYA QTSPAEIMAI PGVDIVVGTQ
110 120 130 140 150
DREKMLGYID QYREERQPIN GVSNIMKARV YEELDVPAFT DRTRASLKIQ
160 170 180 190 200
EGCNNFCTFC IIPWARGLLR SRDPEEVIKQ AQQLVDAGYK EIVLTGIHTG
210 220 230 240 250
GYGEDMKDYN FAKLLSELDT RVEGVKRIRI SSIEASQITD EVIEVLDRSD
260 270 280 290 300
KIVNHLHIPI QSGSNTVLKR MRRKYTMEFF ADRLNKLKKA LPGLAVTSDV
310 320 330 340 350
IVGFPGETEE EFMETYNFIK EHKFSELHVF PYSKRTGTPA ARMEDQVDEN
360 370 380 390 400
VKNERVHRLI ALSDQLAKEY ASQYENEVLE IIPEEAFKET EEENMFVGYT
410 420 430 440 450
DNYMKVVFKG TEDMIGKIVK VKILKAGYPY NEGQFVRVVE DEITEHMRLS

S
Length:451
Mass (Da):51,658
Last modified:October 1, 1996 - v1
Checksum:i9FD45437F5CE3552
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84432 Genomic DNA. Translation: BAA12468.1.
D83717 Genomic DNA. Translation: BAA12080.1.
AL009126 Genomic DNA. Translation: CAB14485.1.
PIRiE69952.
RefSeqiNP_390421.1. NC_000964.3.
WP_003230017.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14485; CAB14485; BSU25430.
GeneIDi937857.
KEGGibsu:BSU25430.
PATRICi18976924. VBIBacSub10457_2651.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84432 Genomic DNA. Translation: BAA12468.1.
D83717 Genomic DNA. Translation: BAA12080.1.
AL009126 Genomic DNA. Translation: CAB14485.1.
PIRiE69952.
RefSeqiNP_390421.1. NC_000964.3.
WP_003230017.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliP54462.
SMRiP54462. Positions 141-436.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100013906.

Proteomic databases

PaxDbiP54462.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14485; CAB14485; BSU25430.
GeneIDi937857.
KEGGibsu:BSU25430.
PATRICi18976924. VBIBacSub10457_2651.

Phylogenomic databases

eggNOGiENOG4105CIW. Bacteria.
COG0621. LUCA.
HOGENOMiHOG000224765.
InParanoidiP54462.
KOiK18707.
OMAiDCSYCIT.
PhylomeDBiP54462.

Enzyme and pathway databases

BioCyciBSUB:BSU25430-MONOMER.
BRENDAi2.8.4.5. 658.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006467. MiaB-like_C.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01579. MiaB-like-C. 1 hit.
TIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTAB_BACSU
AccessioniPrimary (citable) accession number: P54462
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.