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P54450 (CWLH_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-acetylmuramoyl-L-alanine amidase CwlH

EC=3.5.1.28
Alternative name(s):
Autolysin
Cell wall hydrolase
Gene names
Name:cwlH
Synonyms:yqeE
Ordered Locus Names:BSU25710
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length250 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC.

Catalytic activity

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Subcellular location

Secreted Potential.

Developmental stage

Expressed during the late sporulation phase.

Induction

Expression is GerE and sigma K-dependent.

Sequence similarities

Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.0.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4444 Potential
Chain45 – 250206N-acetylmuramoyl-L-alanine amidase CwlH
PRO_0000006454

Sequences

Sequence LengthMass (Da)Tools
P54450 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: E88E0AAD66533FC2

FASTA25027,571
        10         20         30         40         50         60 
MVTIKKDFIP VSNDNRPGYA MAPAYITVHN TANTAKGADA KMHAKFVKNP NTSESWHFTV 

        70         80         90        100        110        120 
DDSVIYQHLP IDENGWHAGD GTNGTGNRKS IGIEICENAD GDFEKATSNA QWLIRKLMKE 

       130        140        150        160        170        180 
NNIPLNRVVP HKKWSGKECP RKLLDHWNSF LNGISSSDTP PKETSPSYPL PSGVIKLTSP 

       190        200        210        220        230        240 
YRKGTNILQL QKALAVLHFY PDKGAKNNGI DGVYGPKTAN AVKRFQLMNG LTADGIYGPK 

       250 
TKAKLKSKLK 

« Hide

References

« Hide 'large scale' references
[1]"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / JH642.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Characterization of a new sigma-K-dependent peptidoglycan hydrolase gene that plays a role in Bacillus subtilis mother cell lysis."
Nugroho F.A., Yamamoto H., Kobayashi Y., Sekiguchi J.
J. Bacteriol. 181:6230-6237(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D84432 Genomic DNA. Translation: BAA12441.1.
AL009126 Genomic DNA. Translation: CAB14512.1.
PIRA69951.
RefSeqNP_390448.1. NC_000964.3.

3D structure databases

ProteinModelPortalP54450.
SMRP54450. Positions 2-154, 171-249.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU25710.

Proteomic databases

PaxDbP54450.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB14512; CAB14512; BSU25710.
GeneID937808.
KEGGbsu:BSU25710.
PATRIC18976984. VBIBacSub10457_2681.

Organism-specific databases

GenoListBSU25710. [Micado]

Phylogenomic databases

eggNOGCOG3409.
HOGENOMHOG000273688.
KOK01447.
OMADENGWHA.
OrthoDBEOG67T5NN.
ProtClustDBCLSK887143.

Enzyme and pathway databases

BioCycBSUB:BSU25710-MONOMER.

Family and domain databases

Gene3D1.10.101.10. 1 hit.
3.40.80.10. 1 hit.
InterProIPR002502. Amidase_domain.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamPF01510. Amidase_2. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
SMARTSM00644. Ami_2. 1 hit.
[Graphical view]
SUPFAMSSF47090. SSF47090. 1 hit.
SSF55846. SSF55846. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCWLH_BACSU
AccessionPrimary (citable) accession number: P54450
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 13, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList