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P54450

- CWLH_BACSU

UniProt

P54450 - CWLH_BACSU

Protein

N-acetylmuramoyl-L-alanine amidase CwlH

Gene

cwlH

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC.

    Catalytic activityi

    Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

    pH dependencei

    Optimum pH is 7.0.

    GO - Molecular functioni

    1. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. establishment of competence for transformation Source: UniProtKB-KW
    2. peptidoglycan catabolic process Source: InterPro
    3. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation, Competence, Sporulation

    Enzyme and pathway databases

    BioCyciBSUB:BSU25710-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acetylmuramoyl-L-alanine amidase CwlH (EC:3.5.1.28)
    Alternative name(s):
    Autolysin
    Cell wall hydrolase
    Gene namesi
    Name:cwlH
    Synonyms:yqeE
    Ordered Locus Names:BSU25710
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU25710. [Micado]

    Subcellular locationi

    Secreted Curated

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 4444Sequence AnalysisAdd
    BLAST
    Chaini45 – 250206N-acetylmuramoyl-L-alanine amidase CwlHPRO_0000006454Add
    BLAST

    Proteomic databases

    PaxDbiP54450.

    Expressioni

    Developmental stagei

    Expressed during the late sporulation phase.

    Inductioni

    Expression is GerE and sigma K-dependent.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU25710.

    Structurei

    3D structure databases

    ProteinModelPortaliP54450.
    SMRiP54450. Positions 2-154, 171-249.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3409.
    HOGENOMiHOG000273688.
    KOiK01447.
    OMAiDENGWHA.
    OrthoDBiEOG67T5NN.
    PhylomeDBiP54450.

    Family and domain databases

    Gene3Di1.10.101.10. 1 hit.
    3.40.80.10. 1 hit.
    InterProiIPR002502. Amidase_domain.
    IPR002477. Peptidoglycan-bd-like.
    [Graphical view]
    PfamiPF01510. Amidase_2. 1 hit.
    PF01471. PG_binding_1. 1 hit.
    [Graphical view]
    SMARTiSM00644. Ami_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF47090. SSF47090. 1 hit.
    SSF55846. SSF55846. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P54450-1 [UniParc]FASTAAdd to Basket

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    MVTIKKDFIP VSNDNRPGYA MAPAYITVHN TANTAKGADA KMHAKFVKNP    50
    NTSESWHFTV DDSVIYQHLP IDENGWHAGD GTNGTGNRKS IGIEICENAD 100
    GDFEKATSNA QWLIRKLMKE NNIPLNRVVP HKKWSGKECP RKLLDHWNSF 150
    LNGISSSDTP PKETSPSYPL PSGVIKLTSP YRKGTNILQL QKALAVLHFY 200
    PDKGAKNNGI DGVYGPKTAN AVKRFQLMNG LTADGIYGPK TKAKLKSKLK 250
    Length:250
    Mass (Da):27,571
    Last modified:October 1, 1996 - v1
    Checksum:iE88E0AAD66533FC2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D84432 Genomic DNA. Translation: BAA12441.1.
    AL009126 Genomic DNA. Translation: CAB14512.1.
    PIRiA69951.
    RefSeqiNP_390448.1. NC_000964.3.
    WP_003229963.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB14512; CAB14512; BSU25710.
    GeneIDi937808.
    KEGGibsu:BSU25710.
    PATRICi18976984. VBIBacSub10457_2681.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D84432 Genomic DNA. Translation: BAA12441.1 .
    AL009126 Genomic DNA. Translation: CAB14512.1 .
    PIRi A69951.
    RefSeqi NP_390448.1. NC_000964.3.
    WP_003229963.1. NZ_CM000487.1.

    3D structure databases

    ProteinModelPortali P54450.
    SMRi P54450. Positions 2-154, 171-249.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU25710.

    Proteomic databases

    PaxDbi P54450.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB14512 ; CAB14512 ; BSU25710 .
    GeneIDi 937808.
    KEGGi bsu:BSU25710.
    PATRICi 18976984. VBIBacSub10457_2681.

    Organism-specific databases

    GenoListi BSU25710. [Micado ]

    Phylogenomic databases

    eggNOGi COG3409.
    HOGENOMi HOG000273688.
    KOi K01447.
    OMAi DENGWHA.
    OrthoDBi EOG67T5NN.
    PhylomeDBi P54450.

    Enzyme and pathway databases

    BioCyci BSUB:BSU25710-MONOMER.

    Family and domain databases

    Gene3Di 1.10.101.10. 1 hit.
    3.40.80.10. 1 hit.
    InterProi IPR002502. Amidase_domain.
    IPR002477. Peptidoglycan-bd-like.
    [Graphical view ]
    Pfami PF01510. Amidase_2. 1 hit.
    PF01471. PG_binding_1. 1 hit.
    [Graphical view ]
    SMARTi SM00644. Ami_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47090. SSF47090. 1 hit.
    SSF55846. SSF55846. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
      Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
      Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / JH642.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "Characterization of a new sigma-K-dependent peptidoglycan hydrolase gene that plays a role in Bacillus subtilis mother cell lysis."
      Nugroho F.A., Yamamoto H., Kobayashi Y., Sekiguchi J.
      J. Bacteriol. 181:6230-6237(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: 168.

    Entry informationi

    Entry nameiCWLH_BACSU
    AccessioniPrimary (citable) accession number: P54450
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3