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Protein

N-acetylmuramoyl-L-alanine amidase CwlH

Gene

cwlH

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC.

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

pH dependencei

Optimum pH is 7.0.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processCell wall biogenesis/degradation, Competence, Sporulation

Enzyme and pathway databases

BioCyciBSUB:BSU25710-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylmuramoyl-L-alanine amidase CwlH (EC:3.5.1.28)
Alternative name(s):
Autolysin
Cell wall hydrolase
Gene namesi
Name:cwlH
Synonyms:yqeE
Ordered Locus Names:BSU25710
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 44Sequence analysisAdd BLAST44
ChainiPRO_000000645445 – 250N-acetylmuramoyl-L-alanine amidase CwlHAdd BLAST206

Proteomic databases

PaxDbiP54450
PRIDEiP54450

Expressioni

Developmental stagei

Expressed during the late sporulation phase.

Inductioni

Expression is GerE and sigma K-dependent.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100014061

Structurei

3D structure databases

ProteinModelPortaliP54450
SMRiP54450
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108VPC Bacteria
COG3409 LUCA
COG5632 LUCA
HOGENOMiHOG000273688
InParanoidiP54450
KOiK01447
OMAiDENGWHA
PhylomeDBiP54450

Family and domain databases

CDDicd06583 PGRP, 1 hit
Gene3Di1.10.101.10, 1 hit
3.40.80.10, 1 hit
InterProiView protein in InterPro
IPR036505 Amidase/PGRP_sf
IPR002502 Amidase_domain
IPR002477 Peptidoglycan-bd-like
IPR036365 PGBD-like_sf
IPR036366 PGBDSf
PfamiView protein in Pfam
PF01510 Amidase_2, 1 hit
PF01471 PG_binding_1, 1 hit
SMARTiView protein in SMART
SM00644 Ami_2, 1 hit
SUPFAMiSSF47090 SSF47090, 1 hit
SSF55846 SSF55846, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54450-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTIKKDFIP VSNDNRPGYA MAPAYITVHN TANTAKGADA KMHAKFVKNP
60 70 80 90 100
NTSESWHFTV DDSVIYQHLP IDENGWHAGD GTNGTGNRKS IGIEICENAD
110 120 130 140 150
GDFEKATSNA QWLIRKLMKE NNIPLNRVVP HKKWSGKECP RKLLDHWNSF
160 170 180 190 200
LNGISSSDTP PKETSPSYPL PSGVIKLTSP YRKGTNILQL QKALAVLHFY
210 220 230 240 250
PDKGAKNNGI DGVYGPKTAN AVKRFQLMNG LTADGIYGPK TKAKLKSKLK
Length:250
Mass (Da):27,571
Last modified:October 1, 1996 - v1
Checksum:iE88E0AAD66533FC2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84432 Genomic DNA Translation: BAA12441.1
AL009126 Genomic DNA Translation: CAB14512.1
PIRiA69951
RefSeqiNP_390448.1, NC_000964.3
WP_003229963.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB14512; CAB14512; BSU25710
GeneIDi937808
KEGGibsu:BSU25710
PATRICifig|224308.179.peg.2796

Entry informationi

Entry nameiCWLH_BACSU
AccessioniPrimary (citable) accession number: P54450
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

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