P54424 (GUN1_USTMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 1
EC=3.2.1.4

Alternative name(s):
Cellulase 1
Endo-1,4-beta-glucanase 1
Short name=EG 1

Gene names

Name:	EGL1
ORF Names:	UM06332

Organism

Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) [Reference proteome]

Taxonomic identifier

237631 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago › Ustilago maydis

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted.
Tissue specificity	Hyphal tip.
Developmental stage	Expressed in filamentous dikaryon.
Post-translational modification	May also be O-glycosylated.
Sequence similarities	Belongs to the glycosyl hydrolase 45 (cellulase K) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 393	367	Endoglucanase 1		PRO_0000008025
Regions
	Compositional bias	270 – 385	116	Ala/Gly/Ser-rich
Sites
	Active site	34	1	Nucleophile By similarity
	Active site	152	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	343	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P54424 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 65C753C610CD6AD3	FASTA	393	39,594
	10 20 30 40 50 60 MAFKLNIGLL ALSLSLSLVH LDGVRAGMAT RYWDCCLASA SWEGKAPVYA PVDACKADGV 70 80 90 100 110 120 TLIDSKKDPS GQSGCNGGNK FMCSCMQPFD DETDPTLAFG FGAFTTGQES DTDCACFYAE 130 140 150 160 170 180 FEHDAQGKAM KRNKLIFQVT NVGGDVQSQN FDFQIPGGGL GAFPKGCPAQ WGVEASLWGD 190 200 210 220 230 240 QYGGVKSATE CSKLPKPLQE GCKWRFSEWG DNPVLKGSPK RVKCPKSLID RSGCQRKDDN 250 260 270 280 290 300 TISPYSGKVD SANTAAPAQY KRDRSVCLAG GKKGKSAAGG VDGSGDASGG ADASGAGGAA 310 320 330 340 350 360 EGSQGQPEGY GQPSGGNDQG SSNGDATTGA GSGSGSDSGS TANGSGSGAP TSGSDGSAVA 370 380 390 PPSGGSNPGA AQGGQGGAQP GPSGGHKKCH KKH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Filament-specific expression of a cellulase gene in the dimorphic fungus Ustilago maydis." Schauwecker F., Wanner G., Kahmann R. Biol. Chem. Hoppe-Seyler 376:617-625(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FBD11.
[2]	"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521 / FGSC 9021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S81598 Genomic DNA. Translation: AAB36147.1. AACP01000242 Genomic DNA. Translation: EAK82961.1.
PIR	S59499.
RefSeq	XP_762479.1. XM_757386.1.
3D structure databases
ProteinModelPortal	P54424.
ModBase	Search...
Protein-protein interaction databases
STRING	5270.UM06332.1.
Protein family/group databases
CAZy	GH45. Glycoside Hydrolase Family 45.
mycoCLAP	EGL45A_USTMA.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	UM06332T0; UM06332P0; UM06332.
GeneID	3634449.
KEGG	uma:UM06332.1.
Phylogenomic databases
eggNOG	NOG140420.
OrthoDB	EOG42FWSN.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-17192.
Family and domain databases
Gene3D	2.40.40.10. 1 hit.
InterPro	IPR014733. Barwin-like_endoglucanase. IPR009009. Barwin-related_endoglucanase. IPR000334. Glyco_hydro_45. [Graphical view]
Pfam	PF02015. Glyco_hydro_45. 1 hit. [Graphical view]
SUPFAM	SSF50685. Barwin_like. 1 hit.
PROSITE	PS01140. GLYCOSYL_HYDROL_F45. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN1_USTMA

Accession

Primary (citable) accession number: P54424
Secondary accession number(s): Q4P0N1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program