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P54424 (GUN1_USTMA) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase 1

EC=3.2.1.4
Alternative name(s):
Cellulase 1
Endo-1,4-beta-glucanase 1
Short name=EG 1
Gene names
Name:EGL1
ORF Names:UM06332
OrganismUstilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) [Reference proteome]
Taxonomic identifier237631 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaBasidiomycotaUstilaginomycotinaUstilaginomycetesUstilaginalesUstilaginaceaeUstilago

Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Subcellular location

Secreted.

Tissue specificity

Hyphal tip.

Developmental stage

Expressed in filamentous dikaryon.

Post-translational modification

May also be O-glycosylated.

Sequence similarities

Belongs to the glycosyl hydrolase 45 (cellulase K) family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 393367Endoglucanase 1
PRO_0000008025

Regions

Compositional bias270 – 385116Ala/Gly/Ser-rich

Sites

Active site341Nucleophile By similarity
Active site1521Proton donor By similarity

Amino acid modifications

Glycosylation3431N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P54424 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 65C753C610CD6AD3

FASTA39339,594
        10         20         30         40         50         60 
MAFKLNIGLL ALSLSLSLVH LDGVRAGMAT RYWDCCLASA SWEGKAPVYA PVDACKADGV 

        70         80         90        100        110        120 
TLIDSKKDPS GQSGCNGGNK FMCSCMQPFD DETDPTLAFG FGAFTTGQES DTDCACFYAE 

       130        140        150        160        170        180 
FEHDAQGKAM KRNKLIFQVT NVGGDVQSQN FDFQIPGGGL GAFPKGCPAQ WGVEASLWGD 

       190        200        210        220        230        240 
QYGGVKSATE CSKLPKPLQE GCKWRFSEWG DNPVLKGSPK RVKCPKSLID RSGCQRKDDN 

       250        260        270        280        290        300 
TISPYSGKVD SANTAAPAQY KRDRSVCLAG GKKGKSAAGG VDGSGDASGG ADASGAGGAA 

       310        320        330        340        350        360 
EGSQGQPEGY GQPSGGNDQG SSNGDATTGA GSGSGSDSGS TANGSGSGAP TSGSDGSAVA 

       370        380        390 
PPSGGSNPGA AQGGQGGAQP GPSGGHKKCH KKH 

« Hide

References

« Hide 'large scale' references
[1]"Filament-specific expression of a cellulase gene in the dimorphic fungus Ustilago maydis."
Schauwecker F., Wanner G., Kahmann R.
Biol. Chem. Hoppe-Seyler 376:617-625(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: FBD11.
[2]"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis."
Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. expand/collapse author list , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W.
Nature 444:97-101(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 521 / FGSC 9021.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S81598 Genomic DNA. Translation: AAB36147.1.
AACP01000242 Genomic DNA. Translation: EAK82961.1.
PIRS59499.
RefSeqXP_762479.1. XM_757386.1.

3D structure databases

ProteinModelPortalP54424.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5270.UM06332.1.

Protein family/group databases

CAZyGH45. Glycoside Hydrolase Family 45.
mycoCLAPEGL45A_USTMA.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiUM06332T0; UM06332P0; UM06332.
GeneID3634449.
KEGGuma:UM06332.1.

Phylogenomic databases

eggNOGNOG140420.
OrthoDBEOG7712K6.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-17192.

Family and domain databases

Gene3D2.40.40.10. 1 hit.
InterProIPR014733. Barwin-like_endoglucanase.
IPR000334. Glyco_hydro_45.
IPR009009. RlpA-like_DPBB.
[Graphical view]
PfamPF02015. Glyco_hydro_45. 1 hit.
[Graphical view]
SUPFAMSSF50685. SSF50685. 1 hit.
PROSITEPS01140. GLYCOSYL_HYDROL_F45. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN1_USTMA
AccessionPrimary (citable) accession number: P54424
Secondary accession number(s): Q4P0N1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 13, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries