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Protein

Endoglucanase 1

Gene

EGL1

Organism
Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei34 – 341NucleophilePROSITE-ProRule annotation
Active sitei152 – 1521Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17192.

Protein family/group databases

CAZyiGH45. Glycoside Hydrolase Family 45.
mycoCLAPiEGL45A_USTMA.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 1 (EC:3.2.1.4)
Alternative name(s):
Cellulase 1
Endo-1,4-beta-glucanase 1
Short name:
EG 1
Gene namesi
Name:EGL1
ORF Names:UM06332
OrganismiUstilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Taxonomic identifieri237631 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaUstilaginomycotinaUstilaginomycetesUstilaginalesUstilaginaceaeUstilago

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 393367Endoglucanase 1PRO_0000008025Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi343 – 3431N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

May also be O-glycosylated.

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Hyphal tip.

Developmental stagei

Expressed in filamentous dikaryon.

Structurei

3D structure databases

ProteinModelPortaliP54424.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi270 – 385116Ala/Gly/Ser-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG140420.
InParanoidiP54424.
OrthoDBiEOG7712K6.

Family and domain databases

Gene3Di2.40.40.10. 1 hit.
InterProiIPR014733. Barwin-like_endoglucanase.
IPR000334. Glyco_hydro_45.
IPR009009. RlpA-like_DPBB.
[Graphical view]
PfamiPF02015. Glyco_hydro_45. 1 hit.
[Graphical view]
SUPFAMiSSF50685. SSF50685. 1 hit.
PROSITEiPS01140. GLYCOSYL_HYDROL_F45. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54424-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFKLNIGLL ALSLSLSLVH LDGVRAGMAT RYWDCCLASA SWEGKAPVYA
60 70 80 90 100
PVDACKADGV TLIDSKKDPS GQSGCNGGNK FMCSCMQPFD DETDPTLAFG
110 120 130 140 150
FGAFTTGQES DTDCACFYAE FEHDAQGKAM KRNKLIFQVT NVGGDVQSQN
160 170 180 190 200
FDFQIPGGGL GAFPKGCPAQ WGVEASLWGD QYGGVKSATE CSKLPKPLQE
210 220 230 240 250
GCKWRFSEWG DNPVLKGSPK RVKCPKSLID RSGCQRKDDN TISPYSGKVD
260 270 280 290 300
SANTAAPAQY KRDRSVCLAG GKKGKSAAGG VDGSGDASGG ADASGAGGAA
310 320 330 340 350
EGSQGQPEGY GQPSGGNDQG SSNGDATTGA GSGSGSDSGS TANGSGSGAP
360 370 380 390
TSGSDGSAVA PPSGGSNPGA AQGGQGGAQP GPSGGHKKCH KKH
Length:393
Mass (Da):39,594
Last modified:October 1, 1996 - v1
Checksum:i65C753C610CD6AD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S81598 Genomic DNA. Translation: AAB36147.1.
AACP01000242 Genomic DNA. Translation: EAK82961.1.
PIRiS59499.
RefSeqiXP_011388317.1. XM_011390015.1.

Genome annotation databases

GeneIDi23565950.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S81598 Genomic DNA. Translation: AAB36147.1.
AACP01000242 Genomic DNA. Translation: EAK82961.1.
PIRiS59499.
RefSeqiXP_011388317.1. XM_011390015.1.

3D structure databases

ProteinModelPortaliP54424.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH45. Glycoside Hydrolase Family 45.
mycoCLAPiEGL45A_USTMA.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi23565950.

Phylogenomic databases

eggNOGiNOG140420.
InParanoidiP54424.
OrthoDBiEOG7712K6.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17192.

Family and domain databases

Gene3Di2.40.40.10. 1 hit.
InterProiIPR014733. Barwin-like_endoglucanase.
IPR000334. Glyco_hydro_45.
IPR009009. RlpA-like_DPBB.
[Graphical view]
PfamiPF02015. Glyco_hydro_45. 1 hit.
[Graphical view]
SUPFAMiSSF50685. SSF50685. 1 hit.
PROSITEiPS01140. GLYCOSYL_HYDROL_F45. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Filament-specific expression of a cellulase gene in the dimorphic fungus Ustilago maydis."
    Schauwecker F., Wanner G., Kahmann R.
    Biol. Chem. Hoppe-Seyler 376:617-625(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: FBD11.
  2. "Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis."
    Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J.
    , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W.
    Nature 444:97-101(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 521 / FGSC 9021.

Entry informationi

Entry nameiGUN1_USTMA
AccessioniPrimary (citable) accession number: P54424
Secondary accession number(s): Q4P0N1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 22, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.