Reviewed, UniProtKB/Swiss-Prot P54413 (CLPP_CAMJE)

Names and origin

Protein names

Recommended name:
    ATP-dependent Clp protease proteolytic subunit
    EC=3.4.21.92
Alternative name(s):
    Endopeptidase Clp

Gene names

Name:	clpP
Ordered Locus Names:	Cj0192c

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity.
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
fliG	Q0PBJ0	1	EBI-1326702,EBI-1191336
fliM	Q0PC73	1	EBI-1326702,EBI-1191095

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 194	194	ATP-dependent Clp protease proteolytic subunit HAMAP MF_00444		PRO_0000179524
Sites
	Active site	97	1	By similarity
	Active site	122	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54413-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: E0B520BD0398093D	FASTA	194	21,650
	10 20 30 40 50 60 MFIPYVIEKS SRGERSYDIY SRLLKDRIIM LSGEIHDELA ASIVAQLLFL EAEDPTKDIY 70 80 90 100 110 120 LYINSPGGVI TSGFSIYDTM NYIKPDVCTI CIGQAASMGA FLLSCGAEGK RFALPNSRIM 130 140 150 160 170 180 IHQPLGGARG QATDIEIQAK EILRLKTILN DILAKNTKQK VAKIAKDTER DFFMSAQEAK 190 EYGLIDKVLE KSFK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.
[2]	"The gene for Campylobacter trigger factor: evidence for multiple transcription start sites and protein products." Griffiths P.L., Park R.W.A., Connerton I.F. Microbiology 141:1359-1367(1995) [PubMed: 7670637] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-49. Strain: NCTC 11168 / Serotype O:2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL111168 Genomic DNA. Translation: CAL34361.1. X85954 Genomic DNA. Translation: CAA59932.1.
PIR	G81437.
RefSeq	YP_002343650.1.
3D structure databases
HSSP	HSSP built from PDB template 1TYF based on UniProtKB P19245.
ModBase	Search...
Protein-protein interaction databases
IntAct	P54413. 97 interactions.
Genome annotation databases
GeneID	906014.
GenomeReviews	Gene locus Cj0192c in contig AL111168_GR.
KEGG	cje:Cj0192c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P54413.
OMA	P54413. PDIVTIC.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0192C-MON.
BRENDA	3.4.21.92. 255835.
Family and domain databases
HAMAP	MF_00444. [Tree]
InterPro	IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_CS. [Graphical view]
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
TIGRFAMs	TIGR00493. clpP. 1 hit.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP_CAMJE

Accession

Primary (citable) accession number: P54413
Secondary accession number(s): Q0PBU7, Q9PIT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)