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P54399

- PDI_DROME

UniProt

P54399 - PDI_DROME

Protein

Protein disulfide-isomerase

Gene

Pdi

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Participates in the folding of proteins containing disulfide bonds.By similarity

    Catalytic activityi

    Catalyzes the rearrangement of -S-S- bonds in proteins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei56 – 561NucleophileBy similarity
    Sitei57 – 571Contributes to redox potential valueBy similarity
    Sitei58 – 581Contributes to redox potential valueBy similarity
    Active sitei59 – 591NucleophileBy similarity
    Sitei120 – 1201Lowers pKa of C-terminal Cys of first active siteBy similarity
    Active sitei397 – 3971NucleophileBy similarity
    Sitei398 – 3981Contributes to redox potential valueBy similarity
    Sitei399 – 3991Contributes to redox potential valueBy similarity
    Active sitei400 – 4001NucleophileBy similarity
    Sitei460 – 4601Lowers pKa of C-terminal Cys of second active siteBy similarity

    GO - Molecular functioni

    1. protein disulfide isomerase activity Source: RefGenome

    GO - Biological processi

    1. cell redox homeostasis Source: InterPro
    2. peptidyl-proline hydroxylation Source: GOC
    3. protein folding Source: GOC
    4. regulation of oxidative stress-induced intrinsic apoptotic signaling pathway Source: RefGenome
    5. response to endoplasmic reticulum stress Source: RefGenome

    Keywords - Molecular functioni

    Isomerase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein disulfide-isomerase (EC:5.3.4.1)
    Short name:
    PDI
    Short name:
    dPDI
    Gene namesi
    Name:Pdi
    ORF Names:CG6988
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3L

    Organism-specific databases

    FlyBaseiFBgn0014002. Pdi.

    Subcellular locationi

    Endoplasmic reticulum lumen PROSITE-ProRule annotation

    GO - Cellular componenti

    1. cell pole Source: FlyBase
    2. cytoplasm Source: FlyBase
    3. endoplasmic reticulum Source: FlyBase
    4. endoplasmic reticulum lumen Source: UniProtKB-SubCell
    5. extracellular space Source: FlyBase
    6. fusome Source: FlyBase
    7. lipid particle Source: FlyBase
    8. neuronal cell body Source: FlyBase
    9. nuclear envelope Source: FlyBase
    10. perinuclear region of cytoplasm Source: FlyBase
    11. rough endoplasmic reticulum Source: FlyBase
    12. spindle envelope Source: FlyBase

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 496478Protein disulfide-isomerasePRO_0000034202Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi56 ↔ 59Redox-activePROSITE-ProRule annotation
    Disulfide bondi397 ↔ 400Redox-activePROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiP54399.
    PRIDEiP54399.

    Expressioni

    Tissue specificityi

    Expressed in all head and body tissues.1 Publication

    Developmental stagei

    Ubiquitously expressed during development.1 Publication

    Gene expression databases

    BgeeiP54399.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi64973. 82 interactions.
    DIPiDIP-21766N.
    IntActiP54399. 5 interactions.
    MINTiMINT-314239.

    Structurei

    3D structure databases

    ProteinModelPortaliP54399.
    SMRiP54399. Positions 26-474.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini19 – 134116Thioredoxin 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini349 – 474126Thioredoxin 2PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi493 – 4964Prevents secretion from ER

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi482 – 4898Poly-Glu

    Sequence similaritiesi

    Belongs to the protein disulfide isomerase family.Curated
    Contains 2 thioredoxin domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Redox-active center, Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG0526.
    GeneTreeiENSGT00740000115202.
    InParanoidiP54399.
    KOiK09580.
    OMAiCHHFLEG.
    OrthoDBiEOG7VHSX1.
    PhylomeDBiP54399.

    Family and domain databases

    Gene3Di3.40.30.10. 4 hits.
    InterProiIPR005788. Disulphide_isomerase.
    IPR005792. Prot_disulphide_isomerase.
    IPR012336. Thioredoxin-like_fold.
    IPR017937. Thioredoxin_CS.
    IPR013766. Thioredoxin_domain.
    [Graphical view]
    PfamiPF00085. Thioredoxin. 2 hits.
    [Graphical view]
    SUPFAMiSSF52833. SSF52833. 4 hits.
    TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
    TIGR01126. pdi_dom. 2 hits.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS00194. THIOREDOXIN_1. 2 hits.
    PS51352. THIOREDOXIN_2. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform A (identifier: P54399-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKFLICALFL AASYVAASAE AEVKVEEGVL VATVDNFKQL IADNEFVLVE    50
    FYAPWCGHCK ALAPEYAKAA QQLAEKESPI KLAKVDATVE GELAEQYAVR 100
    GYPTLKFFRS GSPVEYSGGR QAADIIAWVT KKTGPPAKDL TSVADAEQFL 150
    KDNEIAIIGF FKDLESEEAK TFTKVANALD SFVFGVSSNA DVIAKYEAKD 200
    NGVVLFKPFD DKKSVFEGEL NEENLKKFAQ VQSLPLIVDF NHESASKIFG 250
    GSIKSHLLFF VSREGGHIEK YVDPLKEIAK KYRDDILFVT ISSDEEDHTR 300
    IFEFFGMNKE EVPTIRLIKL EEDMAKYKPE SDDLSAETIE AFLKKFLDGK 350
    LKQHLLSQEL PEDWDKNPVK VLVSSNFESV ALDKSKSVLV EFYAPWCGHC 400
    KQLAPIYDQL AEKYKDNEDI VIAKMDSTAN ELESIKISSF PTIKYFRKED 450
    NKVIDFNLDR TLDDFVKFLD ANGEVADSEP VEETEEEEEA PKKDEL 496
    Length:496
    Mass (Da):55,781
    Last modified:October 1, 1996 - v1
    Checksum:iEB6E04C4216E7A81
    GO
    Isoform D (identifier: P54399-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-306: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:190
    Mass (Da):21,899
    Checksum:iA06DC4C1BC98F510
    GO

    Sequence cautioni

    The sequence AAO24936.1 differs from that shown. Reason: Frameshift at position 362.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti406 – 4061I → T in AAO24936. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 306306Missing in isoform D. 1 PublicationVSP_035858Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18973 mRNA. Translation: AAA86480.1.
    AE014296 Genomic DNA. Translation: AAF49659.1.
    AE014296 Genomic DNA. Translation: AAN11793.1.
    BT001544 mRNA. Translation: AAN71299.1.
    BT003181 mRNA. Translation: AAO24936.1. Frameshift.
    BT011488 mRNA. Translation: AAR99146.1.
    BT012439 mRNA. Translation: AAS93710.1.
    RefSeqiNP_524079.1. NM_079355.3. [P54399-1]
    NP_730033.1. NM_168614.3. [P54399-2]
    UniGeneiDm.2710.

    Genome annotation databases

    EnsemblMetazoaiFBtr0075648; FBpp0075401; FBgn0014002. [P54399-1]
    GeneIDi39651.
    KEGGidme:Dmel_CG6988.
    UCSCiCG6988-RD. d. melanogaster.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18973 mRNA. Translation: AAA86480.1 .
    AE014296 Genomic DNA. Translation: AAF49659.1 .
    AE014296 Genomic DNA. Translation: AAN11793.1 .
    BT001544 mRNA. Translation: AAN71299.1 .
    BT003181 mRNA. Translation: AAO24936.1 . Frameshift.
    BT011488 mRNA. Translation: AAR99146.1 .
    BT012439 mRNA. Translation: AAS93710.1 .
    RefSeqi NP_524079.1. NM_079355.3. [P54399-1 ]
    NP_730033.1. NM_168614.3. [P54399-2 ]
    UniGenei Dm.2710.

    3D structure databases

    ProteinModelPortali P54399.
    SMRi P54399. Positions 26-474.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 64973. 82 interactions.
    DIPi DIP-21766N.
    IntActi P54399. 5 interactions.
    MINTi MINT-314239.

    Proteomic databases

    PaxDbi P54399.
    PRIDEi P54399.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0075648 ; FBpp0075401 ; FBgn0014002 . [P54399-1 ]
    GeneIDi 39651.
    KEGGi dme:Dmel_CG6988.
    UCSCi CG6988-RD. d. melanogaster.

    Organism-specific databases

    CTDi 39651.
    FlyBasei FBgn0014002. Pdi.

    Phylogenomic databases

    eggNOGi COG0526.
    GeneTreei ENSGT00740000115202.
    InParanoidi P54399.
    KOi K09580.
    OMAi CHHFLEG.
    OrthoDBi EOG7VHSX1.
    PhylomeDBi P54399.

    Miscellaneous databases

    ChiTaRSi Pdi. drosophila.
    GenomeRNAii 39651.
    NextBioi 814692.
    PROi P54399.

    Gene expression databases

    Bgeei P54399.

    Family and domain databases

    Gene3Di 3.40.30.10. 4 hits.
    InterProi IPR005788. Disulphide_isomerase.
    IPR005792. Prot_disulphide_isomerase.
    IPR012336. Thioredoxin-like_fold.
    IPR017937. Thioredoxin_CS.
    IPR013766. Thioredoxin_domain.
    [Graphical view ]
    Pfami PF00085. Thioredoxin. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52833. SSF52833. 4 hits.
    TIGRFAMsi TIGR01130. ER_PDI_fam. 1 hit.
    TIGR01126. pdi_dom. 2 hits.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS00194. THIOREDOXIN_1. 2 hits.
    PS51352. THIOREDOXIN_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A Drosophila gene that encodes a member of the protein disulfide isomerase/phospholipase C-alpha family."
      McKay R.R., Zhu L., Shortridge R.D.
      Insect Biochem. Mol. Biol. 25:647-654(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Head.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
      Strain: Berkeley.
      Tissue: Embryo.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM D).
      Strain: Berkeley.
      Tissue: Embryo and Head.

    Entry informationi

    Entry nameiPDI_DROME
    AccessioniPrimary (citable) accession number: P54399
    Secondary accession number(s): Q53YH5
    , Q86PE2, Q8IQL8, Q9VUL7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3