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Protein

Protein disulfide-isomerase

Gene

Pdi

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Participates in the folding of proteins containing disulfide bonds.By similarity

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei56NucleophileBy similarity1
Sitei57Contributes to redox potential valueBy similarity1
Sitei58Contributes to redox potential valueBy similarity1
Active sitei59NucleophileBy similarity1
Sitei120Lowers pKa of C-terminal Cys of first active siteBy similarity1
Active sitei397NucleophileBy similarity1
Sitei398Contributes to redox potential valueBy similarity1
Sitei399Contributes to redox potential valueBy similarity1
Active sitei400NucleophileBy similarity1
Sitei460Lowers pKa of C-terminal Cys of second active siteBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

ReactomeiR-DME-1650814. Collagen biosynthesis and modifying enzymes.
R-DME-174800. Chylomicron-mediated lipid transport.
R-DME-5358346. Hedgehog ligand biogenesis.
R-DME-8866423. VLDL biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase (EC:5.3.4.1)
Short name:
PDI
Short name:
dPDI
Gene namesi
Name:Pdi
ORF Names:CG6988
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0014002. Pdi.

Subcellular locationi

  • Endoplasmic reticulum lumen PROSITE-ProRule annotation

GO - Cellular componenti

  • cell pole Source: FlyBase
  • cytoplasm Source: FlyBase
  • endomembrane system Source: FlyBase
  • endoplasmic reticulum Source: FlyBase
  • endoplasmic reticulum lumen Source: UniProtKB-SubCell
  • extracellular space Source: FlyBase
  • fusome Source: FlyBase
  • lipid particle Source: FlyBase
  • neuronal cell body Source: FlyBase
  • nuclear envelope Source: FlyBase
  • perinuclear region of cytoplasm Source: FlyBase
  • rough endoplasmic reticulum Source: FlyBase
  • spindle envelope Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000003420219 – 496Protein disulfide-isomeraseAdd BLAST478

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi56 ↔ 59Redox-activePROSITE-ProRule annotation
Disulfide bondi397 ↔ 400Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP54399.
PRIDEiP54399.

Expressioni

Tissue specificityi

Expressed in all head and body tissues.1 Publication

Developmental stagei

Ubiquitously expressed during development.1 Publication

Gene expression databases

BgeeiFBgn0014002.
ExpressionAtlasiP54399. baseline.
GenevisibleiP54399. DM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi64973. 83 interactors.
DIPiDIP-21766N.
IntActiP54399. 21 interactors.
MINTiMINT-314239.
STRINGi7227.FBpp0075401.

Structurei

3D structure databases

ProteinModelPortaliP54399.
SMRiP54399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 134Thioredoxin 1PROSITE-ProRule annotationAdd BLAST116
Domaini349 – 474Thioredoxin 2PROSITE-ProRule annotationAdd BLAST126

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi493 – 496Prevents secretion from ER4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi482 – 489Poly-Glu8

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated
Contains 2 thioredoxin domains.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0190. Eukaryota.
COG0526. LUCA.
InParanoidiP54399.
KOiK09580.
OMAiIEKYVDP.
OrthoDBiEOG091G08WM.
PhylomeDBiP54399.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P54399-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKFLICALFL AASYVAASAE AEVKVEEGVL VATVDNFKQL IADNEFVLVE
60 70 80 90 100
FYAPWCGHCK ALAPEYAKAA QQLAEKESPI KLAKVDATVE GELAEQYAVR
110 120 130 140 150
GYPTLKFFRS GSPVEYSGGR QAADIIAWVT KKTGPPAKDL TSVADAEQFL
160 170 180 190 200
KDNEIAIIGF FKDLESEEAK TFTKVANALD SFVFGVSSNA DVIAKYEAKD
210 220 230 240 250
NGVVLFKPFD DKKSVFEGEL NEENLKKFAQ VQSLPLIVDF NHESASKIFG
260 270 280 290 300
GSIKSHLLFF VSREGGHIEK YVDPLKEIAK KYRDDILFVT ISSDEEDHTR
310 320 330 340 350
IFEFFGMNKE EVPTIRLIKL EEDMAKYKPE SDDLSAETIE AFLKKFLDGK
360 370 380 390 400
LKQHLLSQEL PEDWDKNPVK VLVSSNFESV ALDKSKSVLV EFYAPWCGHC
410 420 430 440 450
KQLAPIYDQL AEKYKDNEDI VIAKMDSTAN ELESIKISSF PTIKYFRKED
460 470 480 490
NKVIDFNLDR TLDDFVKFLD ANGEVADSEP VEETEEEEEA PKKDEL
Length:496
Mass (Da):55,781
Last modified:October 1, 1996 - v1
Checksum:iEB6E04C4216E7A81
GO
Isoform D (identifier: P54399-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-306: Missing.

Note: No experimental confirmation available.
Show »
Length:190
Mass (Da):21,899
Checksum:iA06DC4C1BC98F510
GO

Sequence cautioni

The sequence AAO24936 differs from that shown. Reason: Frameshift at position 362.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti406I → T in AAO24936 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0358581 – 306Missing in isoform D. 1 PublicationAdd BLAST306

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18973 mRNA. Translation: AAA86480.1.
AE014296 Genomic DNA. Translation: AAF49659.1.
AE014296 Genomic DNA. Translation: AAN11793.1.
BT001544 mRNA. Translation: AAN71299.1.
BT003181 mRNA. Translation: AAO24936.1. Frameshift.
BT011488 mRNA. Translation: AAR99146.1.
BT012439 mRNA. Translation: AAS93710.1.
RefSeqiNP_001287070.1. NM_001300141.1. [P54399-1]
NP_524079.1. NM_079355.3. [P54399-1]
UniGeneiDm.2710.

Genome annotation databases

EnsemblMetazoaiFBtr0075648; FBpp0075401; FBgn0014002. [P54399-1]
FBtr0346644; FBpp0312224; FBgn0014002. [P54399-1]
GeneIDi39651.
KEGGidme:Dmel_CG6988.
UCSCiCG6988-RD. d. melanogaster.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18973 mRNA. Translation: AAA86480.1.
AE014296 Genomic DNA. Translation: AAF49659.1.
AE014296 Genomic DNA. Translation: AAN11793.1.
BT001544 mRNA. Translation: AAN71299.1.
BT003181 mRNA. Translation: AAO24936.1. Frameshift.
BT011488 mRNA. Translation: AAR99146.1.
BT012439 mRNA. Translation: AAS93710.1.
RefSeqiNP_001287070.1. NM_001300141.1. [P54399-1]
NP_524079.1. NM_079355.3. [P54399-1]
UniGeneiDm.2710.

3D structure databases

ProteinModelPortaliP54399.
SMRiP54399.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi64973. 83 interactors.
DIPiDIP-21766N.
IntActiP54399. 21 interactors.
MINTiMINT-314239.
STRINGi7227.FBpp0075401.

Proteomic databases

PaxDbiP54399.
PRIDEiP54399.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0075648; FBpp0075401; FBgn0014002. [P54399-1]
FBtr0346644; FBpp0312224; FBgn0014002. [P54399-1]
GeneIDi39651.
KEGGidme:Dmel_CG6988.
UCSCiCG6988-RD. d. melanogaster.

Organism-specific databases

CTDi39651.
FlyBaseiFBgn0014002. Pdi.

Phylogenomic databases

eggNOGiKOG0190. Eukaryota.
COG0526. LUCA.
InParanoidiP54399.
KOiK09580.
OMAiIEKYVDP.
OrthoDBiEOG091G08WM.
PhylomeDBiP54399.

Enzyme and pathway databases

ReactomeiR-DME-1650814. Collagen biosynthesis and modifying enzymes.
R-DME-174800. Chylomicron-mediated lipid transport.
R-DME-5358346. Hedgehog ligand biogenesis.
R-DME-8866423. VLDL biosynthesis.

Miscellaneous databases

ChiTaRSiPdi. fly.
GenomeRNAii39651.
PROiP54399.

Gene expression databases

BgeeiFBgn0014002.
ExpressionAtlasiP54399. baseline.
GenevisibleiP54399. DM.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDI_DROME
AccessioniPrimary (citable) accession number: P54399
Secondary accession number(s): Q53YH5
, Q86PE2, Q8IQL8, Q9VUL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.