P54399 (PDI_DROME) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 102.
History...
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Protein disulfide-isomerase Short name=PDI Short name=dPDI EC=5.3.4.1 | ||||
| Gene names |
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| Organism | Drosophila melanogaster (Fruit fly) | ||||
| Taxonomic identifier | 7227 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora |
Protein attributes
| Sequence length | 496 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Participates in the folding of proteins containing disulfide bonds By similarity. |
| Catalytic activity | Catalyzes the rearrangement of -S-S- bonds in proteins. |
| Subunit structure | Homodimer By similarity. |
| Subcellular location | Endoplasmic reticulum lumen Potential. |
| Tissue specificity | Expressed in all head and body tissues. Ref.1 |
| Developmental stage | Ubiquitously expressed during development. Ref.1 |
| Sequence similarities | Belongs to the protein disulfide isomerase family. Contains 2 thioredoxin domains. |
| Sequence caution | The sequence AAO24936.1 differs from that shown. Reason: Frameshift at position 362. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform A (identifier: P54399-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform D (identifier: P54399-2) The sequence of this isoform differs from the canonical sequence as follows: 1-306: Missing. | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 18 | 18 | Potential | ||||||||
| Chain | 19 – 496 | 478 | Protein disulfide-isomerase | PRO_0000034202 | |||||||
Regions | |||||||||||
| Domain | 19 – 134 | 116 | Thioredoxin 1 | ||||||||
| Domain | 349 – 474 | 126 | Thioredoxin 2 | ||||||||
| Motif | 493 – 496 | 4 | Prevents secretion from ER | ||||||||
| Compositional bias | 482 – 489 | 8 | Poly-Glu | ||||||||
Sites | |||||||||||
| Active site | 56 | 1 | Nucleophile By similarity | ||||||||
| Active site | 59 | 1 | Nucleophile By similarity | ||||||||
| Active site | 397 | 1 | Nucleophile By similarity | ||||||||
| Active site | 400 | 1 | Nucleophile By similarity | ||||||||
| Site | 57 | 1 | Contributes to redox potential value By similarity | ||||||||
| Site | 58 | 1 | Contributes to redox potential value By similarity | ||||||||
| Site | 120 | 1 | Lowers pKa of C-terminal Cys of first active site By similarity | ||||||||
| Site | 398 | 1 | Contributes to redox potential value By similarity | ||||||||
| Site | 399 | 1 | Contributes to redox potential value By similarity | ||||||||
| Site | 460 | 1 | Lowers pKa of C-terminal Cys of second active site By similarity | ||||||||
Amino acid modifications | |||||||||||
| Disulfide bond | 56 ↔ 59 | Redox-active By similarity | |||||||||
| Disulfide bond | 397 ↔ 400 | Redox-active By similarity | |||||||||
Natural variations | |||||||||||
| Alternative sequence | 1 – 306 | 306 | Missing in isoform D. | VSP_035858 | |||||||
Experimental info | |||||||||||
| Sequence conflict | 406 | 1 | I → T in AAO24936. Ref.5 | ||||||||
Sequences
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References
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U18973 mRNA. Translation: AAA86480.1. AE014296 Genomic DNA. Translation: AAF49659.1. AE014296 Genomic DNA. Translation: AAN11793.1. BT001544 mRNA. Translation: AAN71299.1. BT003181 mRNA. Translation: AAO24936.1. Frameshift. BT011488 mRNA. Translation: AAR99146.1. BT012439 mRNA. Translation: AAS93710.1. |
| RefSeq | NP_524079.1. NM_079355.2. NP_730033.1. NM_168614.2. |
| UniGene | Dm.2710. |
3D structure databases | |
| ProteinModelPortal | P54399. |
| SMR | P54399. Positions 26-479. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-21766N. |
| IntAct | P54399. 5 interactions. |
| MINT | MINT-314239. |
| STRING | P54399. |
Proteomic databases | |
| PRIDE | P54399. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblMetazoa | FBtr0075648; FBpp0075401; FBgn0014002. |
| GeneID | 39651. |
| KEGG | dme:Dmel_CG6988. |
Organism-specific databases | |
| CTD | 39651. |
| FlyBase | FBgn0014002. Pdi. |
Phylogenomic databases | |
| eggNOG | inNOG05913. |
| GeneTree | EMGT00050000002774. |
| InParanoid | P54399. |
| OMA | DMTKFKP. |
| OrthoDB | EOG4CJSZK. |
| PhylomeDB | P54399. |
Gene expression databases | |
| ArrayExpress | P54399. |
| Bgee | P54399. |
| GermOnline | CG6988. Drosophila melanogaster. |
Family and domain databases | |
| InterPro | IPR005788. Disulphide_isomerase. IPR005792. Prot_disulphide_isomerase. IPR005746. Thioredoxin. IPR012336. Thioredoxin-like_fold. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. [Graphical view] |
| Gene3D | G3DSA:3.40.30.10. Thioredoxin_fold. 4 hits. |
| KO | K09580. |
| Pfam | PF00085. Thioredoxin. 2 hits. [Graphical view] |
| PRINTS | PR00421. THIOREDOXIN. |
| SUPFAM | SSF52833. Thiordxn-like_fd. 4 hits. |
| TIGRFAMs | TIGR01130. ER_PDI_fam. 1 hit. TIGR01126. Pdi_dom. 2 hits. |
| PROSITE | PS00014. ER_TARGET. 1 hit. PS00194. THIOREDOXIN_1. 2 hits. PS51352. THIOREDOXIN_2. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 814692. |
Entry information
| Entry name | PDI_DROME | ||||||||
| Accession | Primary (citable) accession number: P54399 Secondary accession number(s): Q53YH5 Q9VUL7 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Drosophila annotation project | ||||||||
Relevant documents
| Drosophila Drosophila: entries, gene names and cross-references to FlyBase |
| SIMILARITY comments Index of protein domains and families |

Clusters with