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P54399

- PDI_DROME

UniProt

P54399 - PDI_DROME

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Protein

Protein disulfide-isomerase

Gene

Pdi

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Participates in the folding of proteins containing disulfide bonds.By similarity

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei56 – 561NucleophileBy similarity
Sitei57 – 571Contributes to redox potential valueBy similarity
Sitei58 – 581Contributes to redox potential valueBy similarity
Active sitei59 – 591NucleophileBy similarity
Sitei120 – 1201Lowers pKa of C-terminal Cys of first active siteBy similarity
Active sitei397 – 3971NucleophileBy similarity
Sitei398 – 3981Contributes to redox potential valueBy similarity
Sitei399 – 3991Contributes to redox potential valueBy similarity
Active sitei400 – 4001NucleophileBy similarity
Sitei460 – 4601Lowers pKa of C-terminal Cys of second active siteBy similarity

GO - Molecular functioni

  1. protein disulfide isomerase activity Source: RefGenome

GO - Biological processi

  1. cell redox homeostasis Source: InterPro
  2. peptidyl-proline hydroxylation Source: GOC
  3. protein folding Source: GOC
  4. regulation of oxidative stress-induced intrinsic apoptotic signaling pathway Source: RefGenome
  5. response to endoplasmic reticulum stress Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

ReactomeiREACT_180755. Collagen biosynthesis and modifying enzymes.
REACT_205999. Chylomicron-mediated lipid transport.
REACT_209269. Detoxification of Reactive Oxygen Species.
REACT_258701. Hedgehog ligand biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase (EC:5.3.4.1)
Short name:
PDI
Short name:
dPDI
Gene namesi
Name:Pdi
ORF Names:CG6988
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0014002. Pdi.

Subcellular locationi

Endoplasmic reticulum lumen PROSITE-ProRule annotation

GO - Cellular componenti

  1. cell pole Source: FlyBase
  2. cytoplasm Source: FlyBase
  3. endoplasmic reticulum Source: FlyBase
  4. extracellular space Source: FlyBase
  5. fusome Source: FlyBase
  6. lipid particle Source: FlyBase
  7. neuronal cell body Source: FlyBase
  8. nuclear envelope Source: FlyBase
  9. perinuclear region of cytoplasm Source: FlyBase
  10. rough endoplasmic reticulum Source: FlyBase
  11. spindle envelope Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 496478Protein disulfide-isomerasePRO_0000034202Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi56 ↔ 59Redox-activePROSITE-ProRule annotation
Disulfide bondi397 ↔ 400Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP54399.
PRIDEiP54399.

Expressioni

Tissue specificityi

Expressed in all head and body tissues.1 Publication

Developmental stagei

Ubiquitously expressed during development.1 Publication

Gene expression databases

BgeeiP54399.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi64973. 82 interactions.
DIPiDIP-21766N.
IntActiP54399. 5 interactions.
MINTiMINT-314239.

Structurei

3D structure databases

ProteinModelPortaliP54399.
SMRiP54399. Positions 26-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 134116Thioredoxin 1PROSITE-ProRule annotationAdd
BLAST
Domaini349 – 474126Thioredoxin 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi493 – 4964Prevents secretion from ER

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi482 – 4898Poly-Glu

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated
Contains 2 thioredoxin domains.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiCOG0526.
GeneTreeiENSGT00760000119201.
InParanoidiP54399.
KOiK09580.
OMAiCHHFLEG.
OrthoDBiEOG7VHSX1.
PhylomeDBiP54399.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform A (identifier: P54399-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKFLICALFL AASYVAASAE AEVKVEEGVL VATVDNFKQL IADNEFVLVE
60 70 80 90 100
FYAPWCGHCK ALAPEYAKAA QQLAEKESPI KLAKVDATVE GELAEQYAVR
110 120 130 140 150
GYPTLKFFRS GSPVEYSGGR QAADIIAWVT KKTGPPAKDL TSVADAEQFL
160 170 180 190 200
KDNEIAIIGF FKDLESEEAK TFTKVANALD SFVFGVSSNA DVIAKYEAKD
210 220 230 240 250
NGVVLFKPFD DKKSVFEGEL NEENLKKFAQ VQSLPLIVDF NHESASKIFG
260 270 280 290 300
GSIKSHLLFF VSREGGHIEK YVDPLKEIAK KYRDDILFVT ISSDEEDHTR
310 320 330 340 350
IFEFFGMNKE EVPTIRLIKL EEDMAKYKPE SDDLSAETIE AFLKKFLDGK
360 370 380 390 400
LKQHLLSQEL PEDWDKNPVK VLVSSNFESV ALDKSKSVLV EFYAPWCGHC
410 420 430 440 450
KQLAPIYDQL AEKYKDNEDI VIAKMDSTAN ELESIKISSF PTIKYFRKED
460 470 480 490
NKVIDFNLDR TLDDFVKFLD ANGEVADSEP VEETEEEEEA PKKDEL
Length:496
Mass (Da):55,781
Last modified:October 1, 1996 - v1
Checksum:iEB6E04C4216E7A81
GO
Isoform D (identifier: P54399-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-306: Missing.

Note: No experimental confirmation available.

Show »
Length:190
Mass (Da):21,899
Checksum:iA06DC4C1BC98F510
GO

Sequence cautioni

The sequence AAO24936.1 differs from that shown. Reason: Frameshift at position 362. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti406 – 4061I → T in AAO24936. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 306306Missing in isoform D. 1 PublicationVSP_035858Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18973 mRNA. Translation: AAA86480.1.
AE014296 Genomic DNA. Translation: AAF49659.1.
AE014296 Genomic DNA. Translation: AAN11793.1.
BT001544 mRNA. Translation: AAN71299.1.
BT003181 mRNA. Translation: AAO24936.1. Frameshift.
BT011488 mRNA. Translation: AAR99146.1.
BT012439 mRNA. Translation: AAS93710.1.
RefSeqiNP_001287070.1. NM_001300141.1. [P54399-1]
NP_524079.1. NM_079355.3. [P54399-1]
UniGeneiDm.2710.

Genome annotation databases

EnsemblMetazoaiFBtr0075648; FBpp0075401; FBgn0014002. [P54399-1]
GeneIDi39651.
KEGGidme:Dmel_CG6988.
UCSCiCG6988-RD. d. melanogaster.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18973 mRNA. Translation: AAA86480.1 .
AE014296 Genomic DNA. Translation: AAF49659.1 .
AE014296 Genomic DNA. Translation: AAN11793.1 .
BT001544 mRNA. Translation: AAN71299.1 .
BT003181 mRNA. Translation: AAO24936.1 . Frameshift.
BT011488 mRNA. Translation: AAR99146.1 .
BT012439 mRNA. Translation: AAS93710.1 .
RefSeqi NP_001287070.1. NM_001300141.1. [P54399-1 ]
NP_524079.1. NM_079355.3. [P54399-1 ]
UniGenei Dm.2710.

3D structure databases

ProteinModelPortali P54399.
SMRi P54399. Positions 26-474.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 64973. 82 interactions.
DIPi DIP-21766N.
IntActi P54399. 5 interactions.
MINTi MINT-314239.

Proteomic databases

PaxDbi P54399.
PRIDEi P54399.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0075648 ; FBpp0075401 ; FBgn0014002 . [P54399-1 ]
GeneIDi 39651.
KEGGi dme:Dmel_CG6988.
UCSCi CG6988-RD. d. melanogaster.

Organism-specific databases

CTDi 39651.
FlyBasei FBgn0014002. Pdi.

Phylogenomic databases

eggNOGi COG0526.
GeneTreei ENSGT00760000119201.
InParanoidi P54399.
KOi K09580.
OMAi CHHFLEG.
OrthoDBi EOG7VHSX1.
PhylomeDBi P54399.

Enzyme and pathway databases

Reactomei REACT_180755. Collagen biosynthesis and modifying enzymes.
REACT_205999. Chylomicron-mediated lipid transport.
REACT_209269. Detoxification of Reactive Oxygen Species.
REACT_258701. Hedgehog ligand biogenesis.

Miscellaneous databases

ChiTaRSi Pdi. fly.
GenomeRNAii 39651.
NextBioi 814692.
PROi P54399.

Gene expression databases

Bgeei P54399.

Family and domain databases

Gene3Di 3.40.30.10. 4 hits.
InterProi IPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view ]
Pfami PF00085. Thioredoxin. 2 hits.
[Graphical view ]
SUPFAMi SSF52833. SSF52833. 4 hits.
TIGRFAMsi TIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 2 hits.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A Drosophila gene that encodes a member of the protein disulfide isomerase/phospholipase C-alpha family."
    McKay R.R., Zhu L., Shortridge R.D.
    Insect Biochem. Mol. Biol. 25:647-654(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Head.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Strain: Berkeley.
    Tissue: Embryo.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM D).
    Strain: Berkeley.
    Tissue: Embryo and Head.

Entry informationi

Entry nameiPDI_DROME
AccessioniPrimary (citable) accession number: P54399
Secondary accession number(s): Q53YH5
, Q86PE2, Q8IQL8, Q9VUL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3