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Protein

Protein disulfide-isomerase

Gene

Pdi

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Participates in the folding of proteins containing disulfide bonds.By similarity

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei56NucleophileBy similarity1
Sitei57Contributes to redox potential valueBy similarity1
Sitei58Contributes to redox potential valueBy similarity1
Active sitei59NucleophileBy similarity1
Sitei120Lowers pKa of C-terminal Cys of first active siteBy similarity1
Active sitei397NucleophileBy similarity1
Sitei398Contributes to redox potential valueBy similarity1
Sitei399Contributes to redox potential valueBy similarity1
Active sitei400NucleophileBy similarity1
Sitei460Lowers pKa of C-terminal Cys of second active siteBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase

Enzyme and pathway databases

ReactomeiR-DME-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
R-DME-5358346. Hedgehog ligand biogenesis.
R-DME-8866423. VLDL assembly.
R-DME-8957275. Post-translational protein phosphorylation.
R-DME-8963888. Chylomicron assembly.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase (EC:5.3.4.1)
Short name:
PDI
Short name:
dPDI
Gene namesi
Name:Pdi
ORF Names:CG6988
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0014002. Pdi.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000003420219 – 496Protein disulfide-isomeraseAdd BLAST478

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi56 ↔ 59Redox-activePROSITE-ProRule annotation
Disulfide bondi397 ↔ 400Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP54399.
PRIDEiP54399.

Expressioni

Tissue specificityi

Expressed in all head and body tissues.1 Publication

Developmental stagei

Ubiquitously expressed during development.1 Publication

Gene expression databases

BgeeiFBgn0014002.
ExpressionAtlasiP54399. differential.
GenevisibleiP54399. DM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi64973. 85 interactors.
DIPiDIP-21766N.
IntActiP54399. 21 interactors.
MINTiMINT-314239.
STRINGi7227.FBpp0075401.

Structurei

3D structure databases

ProteinModelPortaliP54399.
SMRiP54399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 134Thioredoxin 1PROSITE-ProRule annotationAdd BLAST116
Domaini349 – 474Thioredoxin 2PROSITE-ProRule annotationAdd BLAST126

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi493 – 496Prevents secretion from ER4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi482 – 489Poly-Glu8

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0190. Eukaryota.
COG0526. LUCA.
InParanoidiP54399.
KOiK09580.
OMAiQYGVRGY.
OrthoDBiEOG091G08WM.
PhylomeDBiP54399.

Family and domain databases

InterProiView protein in InterPro
IPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR036249. Thioredoxin-like_sf.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
PfamiView protein in Pfam
PF00085. Thioredoxin. 2 hits.
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 2 hits.
PROSITEiView protein in PROSITE
PS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P54399-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKFLICALFL AASYVAASAE AEVKVEEGVL VATVDNFKQL IADNEFVLVE
60 70 80 90 100
FYAPWCGHCK ALAPEYAKAA QQLAEKESPI KLAKVDATVE GELAEQYAVR
110 120 130 140 150
GYPTLKFFRS GSPVEYSGGR QAADIIAWVT KKTGPPAKDL TSVADAEQFL
160 170 180 190 200
KDNEIAIIGF FKDLESEEAK TFTKVANALD SFVFGVSSNA DVIAKYEAKD
210 220 230 240 250
NGVVLFKPFD DKKSVFEGEL NEENLKKFAQ VQSLPLIVDF NHESASKIFG
260 270 280 290 300
GSIKSHLLFF VSREGGHIEK YVDPLKEIAK KYRDDILFVT ISSDEEDHTR
310 320 330 340 350
IFEFFGMNKE EVPTIRLIKL EEDMAKYKPE SDDLSAETIE AFLKKFLDGK
360 370 380 390 400
LKQHLLSQEL PEDWDKNPVK VLVSSNFESV ALDKSKSVLV EFYAPWCGHC
410 420 430 440 450
KQLAPIYDQL AEKYKDNEDI VIAKMDSTAN ELESIKISSF PTIKYFRKED
460 470 480 490
NKVIDFNLDR TLDDFVKFLD ANGEVADSEP VEETEEEEEA PKKDEL
Length:496
Mass (Da):55,781
Last modified:October 1, 1996 - v1
Checksum:iEB6E04C4216E7A81
GO
Isoform D (identifier: P54399-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-306: Missing.

Note: No experimental confirmation available.
Show »
Length:190
Mass (Da):21,899
Checksum:iA06DC4C1BC98F510
GO

Sequence cautioni

The sequence AAO24936 differs from that shown. Reason: Frameshift at position 362.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti406I → T in AAO24936 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0358581 – 306Missing in isoform D. 1 PublicationAdd BLAST306

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18973 mRNA. Translation: AAA86480.1.
AE014296 Genomic DNA. Translation: AAF49659.1.
AE014296 Genomic DNA. Translation: AAN11793.1.
BT001544 mRNA. Translation: AAN71299.1.
BT003181 mRNA. Translation: AAO24936.1. Frameshift.
BT011488 mRNA. Translation: AAR99146.1.
BT012439 mRNA. Translation: AAS93710.1.
RefSeqiNP_001287070.1. NM_001300141.1. [P54399-1]
NP_524079.1. NM_079355.3. [P54399-1]
UniGeneiDm.2710.

Genome annotation databases

EnsemblMetazoaiFBtr0075648; FBpp0075401; FBgn0014002. [P54399-1]
FBtr0346644; FBpp0312224; FBgn0014002. [P54399-1]
GeneIDi39651.
KEGGidme:Dmel_CG6988.
UCSCiCG6988-RD. d. melanogaster.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPDI_DROME
AccessioniPrimary (citable) accession number: P54399
Secondary accession number(s): Q53YH5
, Q86PE2, Q8IQL8, Q9VUL7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 25, 2017
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families