ID   DHE4_LACBS              Reviewed;         450 AA.
AC   P54388; B0CZ97;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 2.
DT   16-JUN-2009, entry version 46.
DE   RecName: Full=NADP-specific glutamate dehydrogenase;
DE            Short=NADP-GDH;
DE            EC=1.4.1.4;
DE   AltName: Full=NADP-dependent glutamate dehydrogenase;
GN   Name=GDHA; ORFNames=LACBIDRAFT_292653;
OS   Laccaria bicolor (strain S238N-H82) (Bicoloured deceiver) (Laccaria
OS   laccata var. bicolor).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina;
OC   Homobasidiomycetes; Agaricomycetidae; Agaricales; Tricholomataceae;
OC   Laccaria.
OX   NCBI_TaxID=486041;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Lorillou S.S., Martin F.F.;
RT   "Cloning, sequence analysis and expression of a cDNA encoding NADP-
RT   glutamate dehydrogenase from the ectomycorrhizal basidiomycete
RT   Laccaria bicolor.";
RL   Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=18322534; DOI=10.1038/nature06556;
RA   Martin F., Aerts A., Ahren D., Brun A., Danchin E.G.J., Duchaussoy F.,
RA   Gibon J., Kohler A., Lindquist E., Pereda V., Salamov A.,
RA   Shapiro H.J., Wuyts J., Blaudez D., Buee M., Brokstein P.,
RA   Canbaeck B., Cohen D., Courty P.E., Coutinho P.M., Delaruelle C.,
RA   Detter J.C., Deveau A., DiFazio S., Duplessis S.,
RA   Fraissinet-Tachet L., Lucic E., Frey-Klett P., Fourrey C.,
RA   Feussner I., Gay G., Grimwood J., Hoegger P.J., Jain P., Kilaru S.,
RA   Labbe J., Lin Y.C., Legue V., Le Tacon F., Marmeisse R., Melayah D.,
RA   Montanini B., Muratet M., Nehls U., Niculita-Hirzel H.,
RA   Oudot-Le Secq M.P., Peter M., Quesneville H., Rajashekar B., Reich M.,
RA   Rouhier N., Schmutz J., Yin T., Chalot M., Henrissat B., Kuees U.,
RA   Lucas S., Van de Peer Y., Podila G.K., Polle A., Pukkila P.J.,
RA   Richardson P.M., Rouze P., Sanders I.R., Stajich J.E., Tunlid A.,
RA   Tuskan G., Grigoriev I.V.;
RT   "The genome of Laccaria bicolor provides insights into mycorrhizal
RT   symbiosis.";
RL   Nature 452:88-92(2008).
CC   -!- CATALYTIC ACTIVITY: L-glutamate + H(2)O + NADP(+) = 2-oxoglutarate
CC       + NH(3) + NADPH.
CC   -!- SUBUNIT: Homohexamer (By similarity).
CC   -!- SIMILARITY: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
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DR   EMBL; U31369; AAA82936.1; -; mRNA.
DR   EMBL; DS547094; EDR12114.1; -; Genomic_DNA.
DR   RefSeq; XP_001876378.1; -.
DR   HSSP; P24295; 1AUP.
DR   GeneID; 6072874; -.
DR   KEGG; lbc:LACBIDRAFT_292653; -.
DR   BRENDA; 1.4.1.4; 256691.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004354; F:glutamate dehydrogenase (NADP+) activity; IEA:EC.
DR   GO; GO:0006520; P:amino acid metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR006095; Glu/Leu/Phe/Val_DH.
DR   InterPro; IPR006096; Glu/Leu/Phe/Val_DH_C.
DR   InterPro; IPR006097; Glu/Leu/Phe/Val_DH_dimer.
DR   InterPro; IPR014362; Glu_DH.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR11606:SF2; GLFV_DH; 1.
DR   Pfam; PF00208; ELFV_dehydrog; 1.
DR   Pfam; PF02812; ELFV_dehydrog_N; 1.
DR   PIRSF; PIRSF000185; Glu_DH; 1.
DR   PRINTS; PR00082; GLFDHDRGNASE.
DR   PROSITE; PS00074; GLFV_DEHYDROGENASE; 1.
PE   2: Evidence at transcript level;
KW   NADP; Oxidoreductase.
FT   CHAIN         1    450       NADP-specific glutamate dehydrogenase.
FT                                /FTId=PRO_0000182789.
FT   ACT_SITE    111    111       By similarity.
FT   CONFLICT     17     17       Q -> P (in Ref. 1; AAA82936).
FT   CONFLICT     31     31       E -> G (in Ref. 1; AAA82936).
FT   CONFLICT     38     38       Q -> P (in Ref. 1; AAA82936).
FT   CONFLICT     49     49       V -> I (in Ref. 1; AAA82936).
FT   CONFLICT    231    231       A -> G (in Ref. 1; AAA82936).
FT   CONFLICT    349    349       A -> E (in Ref. 1; AAA82936).
SQ   SEQUENCE   450 AA;  48550 MW;  8FDA699CA3D46945 CRC64;
     MVLPVEPEYE QALSELQNSL KPFLAANPDY EKALEIVQIP ERVLQFRVVW EDDQGKAQVN
     RGFRVQYNSA LGPYKGGLRL HPSVNLSILK FLGFEQTFKN ALTGLSMGGG KGGSDFDPKG
     KSDGEIRRFC TSFMSELFRH IGQDTDVPAG DIGTGAREIG YLFGAYKKLQ NEFVGMLTGK
     GLAWGGSFIR PEATGYGLIY YVEHMIAKAA PEYSLSKPET LVAISGSGNV AQFTALKVIE
     LGATVLSLSD SKGSLIAEKG YTKEFIKEIG QLKLKGGALE SLAQREGYTY HAGKRPWSLL
     PVVHVALPGA TQNEVSKTEA EDLIKAGVRI VAEGSNMGCT EDAIAVFEAS RKAGAGGVWY
     APGKASNCGG VAVSGLEMAQ NSQRLAWTTD QVDQKLKKIM AECYEICLSA GTKWSGEEIK
     DGVLPSLLSG ANVAGFIKVA DAMREHGDWW
//