Reviewed, UniProtKB/Swiss-Prot P54388 (DHE4_LACBS)

Names and origin

Protein names

Recommended name:
    NADP-specific glutamate dehydrogenase
      Short name=NADP-GDH
    EC=1.4.1.4
Alternative name(s):
    NADP-dependent glutamate dehydrogenase

Gene names

Name:	GDHA
ORF Names:	LACBIDRAFT_292653

Organism

Laccaria bicolor (strain S238N-H82) (Bicoloured deceiver) (Laccaria laccata var. bicolor)

Taxonomic identifier

486041 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Tricholomataceae › Laccaria

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	NADP-specific glutamate dehydrogenase		PRO_0000182789
Sites
	Active site	111	1	By similarity
Experimental info
	Sequence conflict	17	1	Q → P in AAA82936. Ref.1
	Sequence conflict	31	1	E → G in AAA82936. Ref.1
	Sequence conflict	38	1	Q → P in AAA82936. Ref.1
	Sequence conflict	49	1	V → I in AAA82936. Ref.1
	Sequence conflict	231	1	A → G in AAA82936. Ref.1
	Sequence conflict	349	1	A → E in AAA82936. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P54388-1 [UniParc]. Last modified May 20, 2008. Version 2. Checksum: 8FDA699CA3D46945	FASTA	450	48,550
	10 20 30 40 50 60 MVLPVEPEYE QALSELQNSL KPFLAANPDY EKALEIVQIP ERVLQFRVVW EDDQGKAQVN 70 80 90 100 110 120 RGFRVQYNSA LGPYKGGLRL HPSVNLSILK FLGFEQTFKN ALTGLSMGGG KGGSDFDPKG 130 140 150 160 170 180 KSDGEIRRFC TSFMSELFRH IGQDTDVPAG DIGTGAREIG YLFGAYKKLQ NEFVGMLTGK 190 200 210 220 230 240 GLAWGGSFIR PEATGYGLIY YVEHMIAKAA PEYSLSKPET LVAISGSGNV AQFTALKVIE 250 260 270 280 290 300 LGATVLSLSD SKGSLIAEKG YTKEFIKEIG QLKLKGGALE SLAQREGYTY HAGKRPWSLL 310 320 330 340 350 360 PVVHVALPGA TQNEVSKTEA EDLIKAGVRI VAEGSNMGCT EDAIAVFEAS RKAGAGGVWY 370 380 390 400 410 420 APGKASNCGG VAVSGLEMAQ NSQRLAWTTD QVDQKLKKIM AECYEICLSA GTKWSGEEIK 430 440 450 DGVLPSLLSG ANVAGFIKVA DAMREHGDWW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequence analysis and expression of a cDNA encoding NADP-glutamate dehydrogenase from the ectomycorrhizal basidiomycete Laccaria bicolor." Lorillou S.S., Martin F.F. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome of Laccaria bicolor provides insights into mycorrhizal symbiosis." Martin F., Aerts A., Ahren D., Brun A., Danchin E.G.J., Duchaussoy F., Gibon J., Kohler A., Lindquist E., Pereda V., Salamov A., Shapiro H.J., Wuyts J., Blaudez D., Buee M., Brokstein P., Canbaeck B., Cohen D. , Courty P.E., Coutinho P.M., Delaruelle C., Detter J.C., Deveau A., DiFazio S., Duplessis S., Fraissinet-Tachet L., Lucic E., Frey-Klett P., Fourrey C., Feussner I., Gay G., Grimwood J., Hoegger P.J., Jain P., Kilaru S., Labbe J., Lin Y.C., Legue V., Le Tacon F., Marmeisse R., Melayah D., Montanini B., Muratet M., Nehls U., Niculita-Hirzel H., Oudot-Le Secq M.P., Peter M., Quesneville H., Rajashekar B., Reich M., Rouhier N., Schmutz J., Yin T., Chalot M., Henrissat B., Kuees U., Lucas S., Van de Peer Y., Podila G.K., Polle A., Pukkila P.J., Richardson P.M., Rouze P., Sanders I.R., Stajich J.E., Tunlid A., Tuskan G., Grigoriev I.V. Nature 452:88-92(2008) [PubMed: 18322534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U31369 mRNA. Translation: AAA82936.1. DS547094 Genomic DNA. Translation: EDR12114.1.
RefSeq	XP_001876378.1.
3D structure databases
HSSP	HSSP built from PDB template 1AUP based on UniProtKB P24295.
ModBase	Search...
Genome annotation databases
GeneID	6072874.
KEGG	lbc:LACBIDRAFT_292653.
Enzyme and pathway databases
BRENDA	1.4.1.4. 256691.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_LACBS

Accession

Primary (citable) accession number: P54388
Secondary accession number(s): B0CZ97

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 20, 2008
Last modified:	September 22, 2009
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)