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Protein

NADP-specific glutamate dehydrogenase

Gene

GDHA

Organism
Laccaria bicolor (strain S238N-H82 / ATCC MYA-4686) (Bicoloured deceiver) (Laccaria laccata var. bicolor)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei111 – 1111PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.4.1.4. 2839.

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
Short name:
NADP-GDH
Alternative name(s):
NADP-dependent glutamate dehydrogenase
Gene namesi
Name:GDHA
ORF Names:LACBIDRAFT_292653
OrganismiLaccaria bicolor (strain S238N-H82 / ATCC MYA-4686) (Bicoloured deceiver) (Laccaria laccata var. bicolor)
Taxonomic identifieri486041 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesTricholomataceaeLaccaria
ProteomesiUP000001194 Componenti: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 450450NADP-specific glutamate dehydrogenasePRO_0000182789Add
BLAST

Proteomic databases

PRIDEiP54388.

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

STRINGi486041.XP_001876378.1.

Structurei

3D structure databases

ProteinModelPortaliP54388.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0334.
InParanoidiP54388.
KOiK00262.
OrthoDBiEOG7XSTPW.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54388-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLPVEPEYE QALSELQNSL KPFLAANPDY EKALEIVQIP ERVLQFRVVW
60 70 80 90 100
EDDQGKAQVN RGFRVQYNSA LGPYKGGLRL HPSVNLSILK FLGFEQTFKN
110 120 130 140 150
ALTGLSMGGG KGGSDFDPKG KSDGEIRRFC TSFMSELFRH IGQDTDVPAG
160 170 180 190 200
DIGTGAREIG YLFGAYKKLQ NEFVGMLTGK GLAWGGSFIR PEATGYGLIY
210 220 230 240 250
YVEHMIAKAA PEYSLSKPET LVAISGSGNV AQFTALKVIE LGATVLSLSD
260 270 280 290 300
SKGSLIAEKG YTKEFIKEIG QLKLKGGALE SLAQREGYTY HAGKRPWSLL
310 320 330 340 350
PVVHVALPGA TQNEVSKTEA EDLIKAGVRI VAEGSNMGCT EDAIAVFEAS
360 370 380 390 400
RKAGAGGVWY APGKASNCGG VAVSGLEMAQ NSQRLAWTTD QVDQKLKKIM
410 420 430 440 450
AECYEICLSA GTKWSGEEIK DGVLPSLLSG ANVAGFIKVA DAMREHGDWW
Length:450
Mass (Da):48,550
Last modified:May 20, 2008 - v2
Checksum:i8FDA699CA3D46945
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171Q → P in AAA82936 (Ref. 1) Curated
Sequence conflicti31 – 311E → G in AAA82936 (Ref. 1) Curated
Sequence conflicti38 – 381Q → P in AAA82936 (Ref. 1) Curated
Sequence conflicti49 – 491V → I in AAA82936 (Ref. 1) Curated
Sequence conflicti231 – 2311A → G in AAA82936 (Ref. 1) Curated
Sequence conflicti349 – 3491A → E in AAA82936 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31369 mRNA. Translation: AAA82936.1.
DS547094 Genomic DNA. Translation: EDR12114.1.
RefSeqiXP_001876378.1. XM_001876343.1.

Genome annotation databases

GeneIDi6072874.
KEGGilbc:LACBIDRAFT_292653.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31369 mRNA. Translation: AAA82936.1.
DS547094 Genomic DNA. Translation: EDR12114.1.
RefSeqiXP_001876378.1. XM_001876343.1.

3D structure databases

ProteinModelPortaliP54388.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi486041.XP_001876378.1.

Proteomic databases

PRIDEiP54388.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi6072874.
KEGGilbc:LACBIDRAFT_292653.

Phylogenomic databases

eggNOGiCOG0334.
InParanoidiP54388.
KOiK00262.
OrthoDBiEOG7XSTPW.

Enzyme and pathway databases

BRENDAi1.4.1.4. 2839.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequence analysis and expression of a cDNA encoding NADP-glutamate dehydrogenase from the ectomycorrhizal basidiomycete Laccaria bicolor."
    Lorillou S.S., Martin F.F.
    Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The genome of Laccaria bicolor provides insights into mycorrhizal symbiosis."
    Martin F., Aerts A., Ahren D., Brun A., Danchin E.G.J., Duchaussoy F., Gibon J., Kohler A., Lindquist E., Pereda V., Salamov A., Shapiro H.J., Wuyts J., Blaudez D., Buee M., Brokstein P., Canbaeck B., Cohen D.
    , Courty P.E., Coutinho P.M., Delaruelle C., Detter J.C., Deveau A., DiFazio S., Duplessis S., Fraissinet-Tachet L., Lucic E., Frey-Klett P., Fourrey C., Feussner I., Gay G., Grimwood J., Hoegger P.J., Jain P., Kilaru S., Labbe J., Lin Y.C., Legue V., Le Tacon F., Marmeisse R., Melayah D., Montanini B., Muratet M., Nehls U., Niculita-Hirzel H., Oudot-Le Secq M.P., Peter M., Quesneville H., Rajashekar B., Reich M., Rouhier N., Schmutz J., Yin T., Chalot M., Henrissat B., Kuees U., Lucas S., Van de Peer Y., Podila G.K., Polle A., Pukkila P.J., Richardson P.M., Rouze P., Sanders I.R., Stajich J.E., Tunlid A., Tuskan G., Grigoriev I.V.
    Nature 452:88-92(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: S238N-H82 / ATCC MYA-4686.

Entry informationi

Entry nameiDHE4_LACBS
AccessioniPrimary (citable) accession number: P54388
Secondary accession number(s): B0CZ97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 20, 2008
Last modified: June 24, 2015
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.