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P54387 (DHE4_AGABI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADP-specific glutamate dehydrogenase

Short name=NADP-GDH
EC=1.4.1.4
Alternative name(s):
NADP-dependent glutamate dehydrogenase
Gene names
Name:gdhA
OrganismAgaricus bisporus (White button mushroom)
Taxonomic identifier5341 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesAgaricaceaeAgaricus

Protein attributes

Sequence length457 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Subunit structure

Homohexamer By similarity.

Sequence similarities

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
   LigandNADP
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological_processcellular amino acid metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionglutamate dehydrogenase (NADP+) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 457457NADP-specific glutamate dehydrogenase
PRO_0000182783

Sites

Active site1111 By similarity

Sequences

Sequence LengthMass (Da)Tools
P54387 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 1BF0E97F67078AC4

FASTA45749,557
        10         20         30         40         50         60 
MVLPHEPEFE QALHELETSL QPFLTTNPQY KKALEIIQVP ERVLQFRVTW EDDQGKPQVN 

        70         80         90        100        110        120 
RGFRVQYNSA LGPYKGGLRL HPTVNLSILK FLGFEQTFKN ALTGLSMGGG KGGSDFDPKG 

       130        140        150        160        170        180 
KSDNEIRRFC VAFMSELFRH IGQDTDVPAG DIGTGAREIG FLFGAYRRLK NEFTGMLTGK 

       190        200        210        220        230        240 
GINWGGSFIR PEATGYGLIY YVEHMIAHAC PEYSLDRPST LVAISGSGNV SQFTALKVIE 

       250        260        270        280        290        300 
LGATVLSLSD SKGSLISEKG YTKEAIEKIA ELKLKGGALE AIVDDLGAGY TYHAGKRPWT 

       310        320        330        340        350        360 
LLPQVHIALP GATQNEVSQE EAEALVKAGT RIVAEGSNMG CTEEAIAIFE NSRRASRAGV 

       370        380        390        400        410        420 
WYAPGKASNC GGVAVSGLEM AQNSQRLAWS TQEVDAKLKS IMAECYQICY TAGSRWSGEK 

       430        440        450 
VAEGVAEGEA LPSLLSGANL AGFIKVADAM KEQGDWW 

« Hide

References

[1]"Nucleotide sequence and expression of the gene encoding NADP+-dependent glutamate dehydrogenase (gdhA) from Agaricus bisporus."
Schaap P.J., Mueller Y., Baars J.J.P., Op den Camp H.J.M., Sonnenberg A.S.M., van Griensven L.J.L.D., Visser J.
Mol. Gen. Genet. 250:339-347(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Horst H39.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X83393 Genomic DNA. Translation: CAA58312.1.
PIRS63608.

3D structure databases

ProteinModelPortalP54387.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP54387.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFPIRSF000185. Glu_DH. 1 hit.
PRINTSPR00082. GLFDHDRGNASE.
SMARTSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHE4_AGABI
AccessionPrimary (citable) accession number: P54387
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families