Reviewed, UniProtKB/Swiss-Prot P54387 (DHE4_AGABI)

Names and origin

Protein names

Recommended name:
    NADP-specific glutamate dehydrogenase
      Short name=NADP-GDH
    EC=1.4.1.4
Alternative name(s):
    NADP-dependent glutamate dehydrogenase

Gene names

Name:

gdhA

Organism

Agaricus bisporus (Common mushroom)

Taxonomic identifier

5341 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Agaricaceae › Agaricus

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-glutamate + H(2)O + NADP(+) = 2-oxoglutarate + NH(3) + NADPH.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	NADP-specific glutamate dehydrogenase		PRO_0000182783
Sites
	Active site	111	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54387-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 1BF0E97F67078AC4	FASTA	457	49,557
	10 20 30 40 50 60 MVLPHEPEFE QALHELETSL QPFLTTNPQY KKALEIIQVP ERVLQFRVTW EDDQGKPQVN 70 80 90 100 110 120 RGFRVQYNSA LGPYKGGLRL HPTVNLSILK FLGFEQTFKN ALTGLSMGGG KGGSDFDPKG 130 140 150 160 170 180 KSDNEIRRFC VAFMSELFRH IGQDTDVPAG DIGTGAREIG FLFGAYRRLK NEFTGMLTGK 190 200 210 220 230 240 GINWGGSFIR PEATGYGLIY YVEHMIAHAC PEYSLDRPST LVAISGSGNV SQFTALKVIE 250 260 270 280 290 300 LGATVLSLSD SKGSLISEKG YTKEAIEKIA ELKLKGGALE AIVDDLGAGY TYHAGKRPWT 310 320 330 340 350 360 LLPQVHIALP GATQNEVSQE EAEALVKAGT RIVAEGSNMG CTEEAIAIFE NSRRASRAGV 370 380 390 400 410 420 WYAPGKASNC GGVAVSGLEM AQNSQRLAWS TQEVDAKLKS IMAECYQICY TAGSRWSGEK 430 440 450 VAEGVAEGEA LPSLLSGANL AGFIKVADAM KEQGDWW

References

[1]

"Nucleotide sequence and expression of the gene encoding NADP+-dependent glutamate dehydrogenase (gdhA) from Agaricus bisporus." Schaap P.J., Mueller Y., Baars J.J.P., Op den Camp H.J.M., Sonnenberg A.S.M., van Griensven L.J.L.D., Visser J. Mol. Gen. Genet. 250:339-347(1996) [PubMed: 8602149] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Horst H39.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X83393 Genomic DNA. Translation: CAA58312.1.
PIR	S63608.
3D structure databases
HSSP	HSSP built from PDB template 1AUP based on UniProtKB P24295.
ModBase	Search...
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DHase. IPR006096. Glu/Leu/Phe/Val_DHase_C. IPR006097. Glu/Leu/Phe/Val_DHase_dimer. IPR014362. Glu_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_AGABI

Accession

Primary (citable) accession number: P54387

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)