ID   DHE4_SYNY3              Reviewed;         428 AA.
AC   P54386;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   16-JUN-2009, entry version 52.
DE   RecName: Full=NADP-specific glutamate dehydrogenase;
DE            Short=NADP-GDH;
DE            EC=1.4.1.4;
GN   Name=gdhA; OrderedLocusNames=slr0710;
OS   Synechocystis sp. (strain PCC 6803).
OC   Bacteria; Cyanobacteria; Chroococcales; Synechocystis.
OX   NCBI_TaxID=1148;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=95306786; PubMed=7787182; DOI=10.1007/BF00042048;
RA   Chavez S., Reyes J.C., Chauvat F., Florencio F.J., Candau P.;
RT   "The NADP-glutamate dehydrogenase of the cyanobacterium Synechocystis
RT   6803: cloning, transcriptional analysis and disruption of the gdhA
RT   gene.";
RL   Plant Mol. Biol. 28:173-188(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=96127529; PubMed=8590279; DOI=10.1093/dnares/2.4.153;
RA   Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N.,
RA   Sugiura M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb
RT   region from map positions 64% to 92% of the genome.";
RL   DNA Res. 2:153-166(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=97061201; PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T.,
RA   Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S.,
RA   Shimpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M.,
RA   Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the
RT   entire genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
CC   -!- CATALYTIC ACTIVITY: L-glutamate + H(2)O + NADP(+) = 2-oxoglutarate
CC       + NH(3) + NADPH.
CC   -!- SUBUNIT: Homohexamer (By similarity).
CC   -!- SIMILARITY: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
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DR   EMBL; X77454; CAA54601.1; -; Genomic_DNA.
DR   EMBL; BA000022; BAA10756.1; -; Genomic_DNA.
DR   PIR; S77064; S77064.
DR   RefSeq; NP_442685.1; -.
DR   HSSP; O74024; 1EUZ.
DR   GeneID; 952177; -.
DR   GenomeReviews; BA000022_GR; slr0710.
DR   KEGG; syn:slr0710; -.
DR   NMPDR; fig|1148.1.peg.2786; -.
DR   HOGENOM; P54386; -.
DR   OMA; P54386; FKCALLN.
DR   BioCyc; SSP1148:SLR0710-MON; -.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004354; F:glutamate dehydrogenase (NADP+) activity; IEA:EC.
DR   GO; GO:0006520; P:amino acid metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR006095; Glu/Leu/Phe/Val_DH.
DR   InterPro; IPR006096; Glu/Leu/Phe/Val_DH_C.
DR   InterPro; IPR006097; Glu/Leu/Phe/Val_DH_dimer.
DR   InterPro; IPR014362; Glu_DH.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR11606:SF2; GLFV_DH; 1.
DR   Pfam; PF00208; ELFV_dehydrog; 1.
DR   Pfam; PF02812; ELFV_dehydrog_N; 1.
DR   PIRSF; PIRSF000185; Glu_DH; 1.
DR   PRINTS; PR00082; GLFDHDRGNASE.
DR   PROSITE; PS00074; GLFV_DEHYDROGENASE; 1.
PE   3: Inferred from homology;
KW   Complete proteome; NADP; Oxidoreductase.
FT   CHAIN         1    428       NADP-specific glutamate dehydrogenase.
FT                                /FTId=PRO_0000182777.
FT   ACT_SITE    104    104       By similarity.
SQ   SEQUENCE   428 AA;  47313 MW;  F966273E8D5AD04E CRC64;
     MAGSLFADAS KRLEKALKYV AISDDAGERL KYPKTSLSVS IPVRMDDGSL KIFPGYRVRY
     DDTRGPGKGG VRYHPNVTMD EVQSLAFWMT FKCALLNLPF GGAKGGITLN PKELSRAELE
     RLSRGYIEAI ADFIGPDIDI LAPDVYTNEM MMGWMMDQYS IIRRKISPAV VTGKPVTMGG
     SQGRNTATGT GAFYIMQGML PKFDQYPENT TVAVQGFGNA GMVVAECLYQ DGYKVVAISD
     SQGGIYNEQG IDIPAVIDYK QRHRTLAGMY CDQAICDLGE NQQISNAELL ALDVDVLIPA
     ALENQITRDN ADQVRARYIF EVANGPTTTA ADDILASKGI YVFPDILVNA GGVTVSYFEW
     VQNRSGLYWS AKEVNDRLKE KMVEEAEHVW NITQELDVNV RTAAYIHALN RLSEAMDAKG
     TRDYYQDS
//