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Protein

NADP-specific glutamate dehydrogenase

Gene

gdhA

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia.By similarity

Catalytic activityi

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei68 – 681SubstrateBy similarity
Binding sitei92 – 921SubstrateBy similarity
Active sitei104 – 1041Proton donorPROSITE-ProRule annotation
Sitei144 – 1441Important for catalysisBy similarity
Binding sitei188 – 1881NADPBy similarity
Binding sitei219 – 2191NADPBy similarity
Binding sitei356 – 3561SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
Short name:
NADP-GDH
Gene namesi
Name:gdhA
Ordered Locus Names:slr0710
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428NADP-specific glutamate dehydrogenasePRO_0000182777Add
BLAST

Proteomic databases

PaxDbiP54386.

Interactioni

Subunit structurei

Homohexamer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP54386.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0334.
HOGENOMiHOG000243801.
InParanoidiP54386.
KOiK00262.
OMAiNEMIMGW.
OrthoDBiEOG65XN4D.
PhylomeDBiP54386.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54386-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGSLFADAS KRLEKALKYV AISDDAGERL KYPKTSLSVS IPVRMDDGSL
60 70 80 90 100
KIFPGYRVRY DDTRGPGKGG VRYHPNVTMD EVQSLAFWMT FKCALLNLPF
110 120 130 140 150
GGAKGGITLN PKELSRAELE RLSRGYIEAI ADFIGPDIDI LAPDVYTNEM
160 170 180 190 200
MMGWMMDQYS IIRRKISPAV VTGKPVTMGG SQGRNTATGT GAFYIMQGML
210 220 230 240 250
PKFDQYPENT TVAVQGFGNA GMVVAECLYQ DGYKVVAISD SQGGIYNEQG
260 270 280 290 300
IDIPAVIDYK QRHRTLAGMY CDQAICDLGE NQQISNAELL ALDVDVLIPA
310 320 330 340 350
ALENQITRDN ADQVRARYIF EVANGPTTTA ADDILASKGI YVFPDILVNA
360 370 380 390 400
GGVTVSYFEW VQNRSGLYWS AKEVNDRLKE KMVEEAEHVW NITQELDVNV
410 420
RTAAYIHALN RLSEAMDAKG TRDYYQDS
Length:428
Mass (Da):47,313
Last modified:October 1, 1996 - v1
Checksum:iF966273E8D5AD04E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77454 Genomic DNA. Translation: CAA54601.1.
BA000022 Genomic DNA. Translation: BAA10756.1.
PIRiS77064.

Genome annotation databases

EnsemblBacteriaiBAA10756; BAA10756; BAA10756.
KEGGisyy:SYNGTS_2793.
syz:MYO_128190.
PATRICi23843116. VBISynSp132158_3096.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77454 Genomic DNA. Translation: CAA54601.1.
BA000022 Genomic DNA. Translation: BAA10756.1.
PIRiS77064.

3D structure databases

ProteinModelPortaliP54386.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiP54386.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA10756; BAA10756; BAA10756.
KEGGisyy:SYNGTS_2793.
syz:MYO_128190.
PATRICi23843116. VBISynSp132158_3096.

Phylogenomic databases

eggNOGiCOG0334.
HOGENOMiHOG000243801.
InParanoidiP54386.
KOiK00262.
OMAiNEMIMGW.
OrthoDBiEOG65XN4D.
PhylomeDBiP54386.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The NADP-glutamate dehydrogenase of the cyanobacterium Synechocystis 6803: cloning, transcriptional analysis and disruption of the gdhA gene."
    Chavez S., Reyes J.C., Chauvat F., Florencio F.J., Candau P.
    Plant Mol. Biol. 28:173-188(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
    Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
    DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27184 / PCC 6803 / N-1.
  3. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiDHE4_SYNY3
AccessioniPrimary (citable) accession number: P54386
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 22, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.