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P54386 (DHE4_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADP-specific glutamate dehydrogenase

Short name=NADP-GDH
EC=1.4.1.4
Gene names
Name:gdhA
Ordered Locus Names:slr0710
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia By similarity.

Catalytic activity

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Subunit structure

Homohexamer By similarity.

Sequence similarities

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamate biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionglutamate dehydrogenase (NADP+) activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 428428NADP-specific glutamate dehydrogenase
PRO_0000182777

Sites

Active site1041Proton donor By similarity
Binding site681Substrate By similarity
Binding site921Substrate By similarity
Binding site1881NADP By similarity
Binding site2191NADP By similarity
Binding site3561Substrate By similarity
Site1441Important for catalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
P54386 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: F966273E8D5AD04E

FASTA42847,313
        10         20         30         40         50         60 
MAGSLFADAS KRLEKALKYV AISDDAGERL KYPKTSLSVS IPVRMDDGSL KIFPGYRVRY 

        70         80         90        100        110        120 
DDTRGPGKGG VRYHPNVTMD EVQSLAFWMT FKCALLNLPF GGAKGGITLN PKELSRAELE 

       130        140        150        160        170        180 
RLSRGYIEAI ADFIGPDIDI LAPDVYTNEM MMGWMMDQYS IIRRKISPAV VTGKPVTMGG 

       190        200        210        220        230        240 
SQGRNTATGT GAFYIMQGML PKFDQYPENT TVAVQGFGNA GMVVAECLYQ DGYKVVAISD 

       250        260        270        280        290        300 
SQGGIYNEQG IDIPAVIDYK QRHRTLAGMY CDQAICDLGE NQQISNAELL ALDVDVLIPA 

       310        320        330        340        350        360 
ALENQITRDN ADQVRARYIF EVANGPTTTA ADDILASKGI YVFPDILVNA GGVTVSYFEW 

       370        380        390        400        410        420 
VQNRSGLYWS AKEVNDRLKE KMVEEAEHVW NITQELDVNV RTAAYIHALN RLSEAMDAKG 


TRDYYQDS 

« Hide

References

« Hide 'large scale' references
[1]"The NADP-glutamate dehydrogenase of the cyanobacterium Synechocystis 6803: cloning, transcriptional analysis and disruption of the gdhA gene."
Chavez S., Reyes J.C., Chauvat F., Florencio F.J., Candau P.
Plant Mol. Biol. 28:173-188(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27184 / PCC 6803 / N-1.
[3]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X77454 Genomic DNA. Translation: CAA54601.1.
BA000022 Genomic DNA. Translation: BAA10756.1.
PIRS77064.
RefSeqNP_442685.1. NC_000911.1.
YP_005652746.1. NC_017277.1.
YP_007452561.1. NC_020286.1.

3D structure databases

ProteinModelPortalP54386.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING1148.slr0710.

Proteomic databases

PaxDbP54386.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA10756; BAA10756; BAA10756.
GeneID952177.
KEGGsyn:slr0710.
syy:SYNGTS_2793.
syz:MYO_128190.
PATRIC23843116. VBISynSp132158_3096.

Phylogenomic databases

eggNOGCOG0334.
HOGENOMHOG000243801.
KOK00262.
OMAHGRESAT.
OrthoDBEOG65XN4D.
PhylomeDBP54386.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFPIRSF000185. Glu_DH. 1 hit.
PRINTSPR00082. GLFDHDRGNASE.
SMARTSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHE4_SYNY3
AccessionPrimary (citable) accession number: P54386
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families