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P54386

- DHE4_SYNY3

UniProt

P54386 - DHE4_SYNY3

Protein

NADP-specific glutamate dehydrogenase

Gene

gdhA

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia.By similarity

    Catalytic activityi

    L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei68 – 681SubstrateBy similarity
    Binding sitei92 – 921SubstrateBy similarity
    Active sitei104 – 1041Proton donorPROSITE-ProRule annotation
    Sitei144 – 1441Important for catalysisBy similarity
    Binding sitei188 – 1881NADPBy similarity
    Binding sitei219 – 2191NADPBy similarity
    Binding sitei356 – 3561SubstrateBy similarity

    GO - Molecular functioni

    1. glutamate dehydrogenase (NADP+) activity Source: UniProtKB

    GO - Biological processi

    1. glutamate biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
    Short name:
    NADP-GDH
    Gene namesi
    Name:gdhA
    Ordered Locus Names:slr0710
    OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
    Taxonomic identifieri1111708 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
    ProteomesiUP000001425: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 428428NADP-specific glutamate dehydrogenasePRO_0000182777Add
    BLAST

    Proteomic databases

    PaxDbiP54386.

    Interactioni

    Subunit structurei

    Homohexamer.By similarity

    Protein-protein interaction databases

    STRINGi1148.slr0710.

    Structurei

    3D structure databases

    ProteinModelPortaliP54386.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0334.
    HOGENOMiHOG000243801.
    KOiK00262.
    OMAiHGRESAT.
    OrthoDBiEOG65XN4D.
    PhylomeDBiP54386.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000185. Glu_DH. 1 hit.
    PRINTSiPR00082. GLFDHDRGNASE.
    SMARTiSM00839. ELFV_dehydrog. 1 hit.
    [Graphical view]
    PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P54386-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGSLFADAS KRLEKALKYV AISDDAGERL KYPKTSLSVS IPVRMDDGSL    50
    KIFPGYRVRY DDTRGPGKGG VRYHPNVTMD EVQSLAFWMT FKCALLNLPF 100
    GGAKGGITLN PKELSRAELE RLSRGYIEAI ADFIGPDIDI LAPDVYTNEM 150
    MMGWMMDQYS IIRRKISPAV VTGKPVTMGG SQGRNTATGT GAFYIMQGML 200
    PKFDQYPENT TVAVQGFGNA GMVVAECLYQ DGYKVVAISD SQGGIYNEQG 250
    IDIPAVIDYK QRHRTLAGMY CDQAICDLGE NQQISNAELL ALDVDVLIPA 300
    ALENQITRDN ADQVRARYIF EVANGPTTTA ADDILASKGI YVFPDILVNA 350
    GGVTVSYFEW VQNRSGLYWS AKEVNDRLKE KMVEEAEHVW NITQELDVNV 400
    RTAAYIHALN RLSEAMDAKG TRDYYQDS 428
    Length:428
    Mass (Da):47,313
    Last modified:October 1, 1996 - v1
    Checksum:iF966273E8D5AD04E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77454 Genomic DNA. Translation: CAA54601.1.
    BA000022 Genomic DNA. Translation: BAA10756.1.
    PIRiS77064.
    RefSeqiNP_442685.1. NC_000911.1.
    YP_005652746.1. NC_017277.1.
    YP_007452561.1. NC_020286.1.

    Genome annotation databases

    EnsemblBacteriaiBAA10756; BAA10756; BAA10756.
    GeneIDi952177.
    KEGGisyn:slr0710.
    syy:SYNGTS_2793.
    syz:MYO_128190.
    PATRICi23843116. VBISynSp132158_3096.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77454 Genomic DNA. Translation: CAA54601.1 .
    BA000022 Genomic DNA. Translation: BAA10756.1 .
    PIRi S77064.
    RefSeqi NP_442685.1. NC_000911.1.
    YP_005652746.1. NC_017277.1.
    YP_007452561.1. NC_020286.1.

    3D structure databases

    ProteinModelPortali P54386.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 1148.slr0710.

    Proteomic databases

    PaxDbi P54386.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAA10756 ; BAA10756 ; BAA10756 .
    GeneIDi 952177.
    KEGGi syn:slr0710.
    syy:SYNGTS_2793.
    syz:MYO_128190.
    PATRICi 23843116. VBISynSp132158_3096.

    Phylogenomic databases

    eggNOGi COG0334.
    HOGENOMi HOG000243801.
    KOi K00262.
    OMAi HGRESAT.
    OrthoDBi EOG65XN4D.
    PhylomeDBi P54386.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000185. Glu_DH. 1 hit.
    PRINTSi PR00082. GLFDHDRGNASE.
    SMARTi SM00839. ELFV_dehydrog. 1 hit.
    [Graphical view ]
    PROSITEi PS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The NADP-glutamate dehydrogenase of the cyanobacterium Synechocystis 6803: cloning, transcriptional analysis and disruption of the gdhA gene."
      Chavez S., Reyes J.C., Chauvat F., Florencio F.J., Candau P.
      Plant Mol. Biol. 28:173-188(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
      Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
      DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27184 / PCC 6803 / N-1.
    3. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
      Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
      , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 6803 / Kazusa.

    Entry informationi

    Entry nameiDHE4_SYNY3
    AccessioniPrimary (citable) accession number: P54386
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Synechocystis PCC 6803
      Synechocystis (strain PCC 6803): entries and gene names

    External Data

    Dasty 3