P54386 (DHE4_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADP-specific glutamate dehydrogenase
Short name=NADP-GDH
EC=1.4.1.4

Gene names

Name:	gdhA
Ordered Locus Names:	slr0710

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	NADP-specific glutamate dehydrogenase		PRO_0000182777
Sites
	Active site	104	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54386 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: F966273E8D5AD04E	FASTA	428	47,313
	10 20 30 40 50 60 MAGSLFADAS KRLEKALKYV AISDDAGERL KYPKTSLSVS IPVRMDDGSL KIFPGYRVRY 70 80 90 100 110 120 DDTRGPGKGG VRYHPNVTMD EVQSLAFWMT FKCALLNLPF GGAKGGITLN PKELSRAELE 130 140 150 160 170 180 RLSRGYIEAI ADFIGPDIDI LAPDVYTNEM MMGWMMDQYS IIRRKISPAV VTGKPVTMGG 190 200 210 220 230 240 SQGRNTATGT GAFYIMQGML PKFDQYPENT TVAVQGFGNA GMVVAECLYQ DGYKVVAISD 250 260 270 280 290 300 SQGGIYNEQG IDIPAVIDYK QRHRTLAGMY CDQAICDLGE NQQISNAELL ALDVDVLIPA 310 320 330 340 350 360 ALENQITRDN ADQVRARYIF EVANGPTTTA ADDILASKGI YVFPDILVNA GGVTVSYFEW 370 380 390 400 410 420 VQNRSGLYWS AKEVNDRLKE KMVEEAEHVW NITQELDVNV RTAAYIHALN RLSEAMDAKG TRDYYQDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The NADP-glutamate dehydrogenase of the cyanobacterium Synechocystis 6803: cloning, transcriptional analysis and disruption of the gdhA gene." Chavez S., Reyes J.C., Chauvat F., Florencio F.J., Candau P. Plant Mol. Biol. 28:173-188(1995) [PubMed: 7787182] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.
[3]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X77454 Genomic DNA. Translation: CAA54601.1. BA000022 Genomic DNA. Translation: BAA10756.1.
PIR	S77064.
RefSeq	NP_442685.1. NC_000911.1.
3D structure databases
ProteinModelPortal	P54386.
ModBase	Search...
Protein-protein interaction databases
STRING	P54386.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	952177.
GenomeReviews	Gene locus slr0710 in contig BA000022_GR.
KEGG	syn:slr0710.
NMPDR	fig|1148.1.peg.2786.
PATRIC	23843116. VBISynSp132158_3096.
Phylogenomic databases
eggNOG	COG0334.
HOGENOM	HBG590661.
OMA	NASKECK.
PhylomeDB	P54386.
ProtClustDB	CLSK893665.
Enzyme and pathway databases
BioCyc	SSP1148:SLR0710-MONOMER.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00262.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
SMART	SM00839. ELFV_dehydrog. 1 hit. [Graphical view]
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_SYNY3

Accession

Primary (citable) accession number: P54386

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program