Reviewed, UniProtKB/Swiss-Prot P54386 (DHE4_SYNY3)

Names and origin

Protein names

Recommended name:
    NADP-specific glutamate dehydrogenase
      Short name=NADP-GDH
    EC=1.4.1.4

Gene names

Name:	gdhA
Ordered Locus Names:	slr0710

Organism

Synechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]

Taxonomic identifier

1148 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-glutamate + H(2)O + NADP(+) = 2-oxoglutarate + NH(3) + NADPH.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	NADP-specific glutamate dehydrogenase		PRO_0000182777
Sites
	Active site	104	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54386-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: F966273E8D5AD04E	FASTA	428	47,313
	10 20 30 40 50 60 MAGSLFADAS KRLEKALKYV AISDDAGERL KYPKTSLSVS IPVRMDDGSL KIFPGYRVRY 70 80 90 100 110 120 DDTRGPGKGG VRYHPNVTMD EVQSLAFWMT FKCALLNLPF GGAKGGITLN PKELSRAELE 130 140 150 160 170 180 RLSRGYIEAI ADFIGPDIDI LAPDVYTNEM MMGWMMDQYS IIRRKISPAV VTGKPVTMGG 190 200 210 220 230 240 SQGRNTATGT GAFYIMQGML PKFDQYPENT TVAVQGFGNA GMVVAECLYQ DGYKVVAISD 250 260 270 280 290 300 SQGGIYNEQG IDIPAVIDYK QRHRTLAGMY CDQAICDLGE NQQISNAELL ALDVDVLIPA 310 320 330 340 350 360 ALENQITRDN ADQVRARYIF EVANGPTTTA ADDILASKGI YVFPDILVNA GGVTVSYFEW 370 380 390 400 410 420 VQNRSGLYWS AKEVNDRLKE KMVEEAEHVW NITQELDVNV RTAAYIHALN RLSEAMDAKG TRDYYQDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The NADP-glutamate dehydrogenase of the cyanobacterium Synechocystis 6803: cloning, transcriptional analysis and disruption of the gdhA gene." Chavez S., Reyes J.C., Chauvat F., Florencio F.J., Candau P. Plant Mol. Biol. 28:173-188(1995) [PubMed: 7787182] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X77454 Genomic DNA. Translation: CAA54601.1. BA000022 Genomic DNA. Translation: BAA10756.1.
PIR	S77064.
RefSeq	NP_442685.1.
3D structure databases
HSSP	HSSP built from PDB template 1EUZ based on UniProtKB O74024.
ModBase	Search...
Genome annotation databases
GeneID	952177.
GenomeReviews	Gene locus slr0710 in contig BA000022_GR.
KEGG	syn:slr0710.
NMPDR	fig|1148.1.peg.2786.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P54386.
Enzyme and pathway databases
BioCyc	SSP1148:SLR0710-MON.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DHase. IPR006096. Glu/Leu/Phe/Val_DHase_C. IPR006097. Glu/Leu/Phe/Val_DHase_dimer. IPR014362. Glu_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_SYNY3

Accession

Primary (citable) accession number: P54386

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)