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Protein

NADP-specific glutamate dehydrogenase

Gene

gdhA

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia.By similarity

Catalytic activityi

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei68SubstrateBy similarity1
Binding sitei92SubstrateBy similarity1
Active sitei104Proton donorPROSITE-ProRule annotation1
Sitei144Important for catalysisBy similarity1
Binding sitei188NADPBy similarity1
Binding sitei219NADPBy similarity1
Binding sitei356SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
Short name:
NADP-GDH
Gene namesi
Name:gdhA
Ordered Locus Names:slr0710
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001827771 – 428NADP-specific glutamate dehydrogenaseAdd BLAST428

Proteomic databases

PRIDEiP54386.

Interactioni

Subunit structurei

Homohexamer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP54386.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000243801.
InParanoidiP54386.
KOiK00262.
OMAiNEMIMGW.
PhylomeDBiP54386.

Family and domain databases

CDDicd01076. NAD_bind_1_Glu_DH. 1 hit.
Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR033524. Glu/Leu/Phe/Val_DH_AS.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
IPR033922. NAD_bind_Glu_DH.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54386-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGSLFADAS KRLEKALKYV AISDDAGERL KYPKTSLSVS IPVRMDDGSL
60 70 80 90 100
KIFPGYRVRY DDTRGPGKGG VRYHPNVTMD EVQSLAFWMT FKCALLNLPF
110 120 130 140 150
GGAKGGITLN PKELSRAELE RLSRGYIEAI ADFIGPDIDI LAPDVYTNEM
160 170 180 190 200
MMGWMMDQYS IIRRKISPAV VTGKPVTMGG SQGRNTATGT GAFYIMQGML
210 220 230 240 250
PKFDQYPENT TVAVQGFGNA GMVVAECLYQ DGYKVVAISD SQGGIYNEQG
260 270 280 290 300
IDIPAVIDYK QRHRTLAGMY CDQAICDLGE NQQISNAELL ALDVDVLIPA
310 320 330 340 350
ALENQITRDN ADQVRARYIF EVANGPTTTA ADDILASKGI YVFPDILVNA
360 370 380 390 400
GGVTVSYFEW VQNRSGLYWS AKEVNDRLKE KMVEEAEHVW NITQELDVNV
410 420
RTAAYIHALN RLSEAMDAKG TRDYYQDS
Length:428
Mass (Da):47,313
Last modified:October 1, 1996 - v1
Checksum:iF966273E8D5AD04E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77454 Genomic DNA. Translation: CAA54601.1.
BA000022 Genomic DNA. Translation: BAA10756.1.
PIRiS77064.

Genome annotation databases

EnsemblBacteriaiBAA10756; BAA10756; BAA10756.
KEGGisyn:slr0710.
PATRICi23843116. VBISynSp132158_3096.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77454 Genomic DNA. Translation: CAA54601.1.
BA000022 Genomic DNA. Translation: BAA10756.1.
PIRiS77064.

3D structure databases

ProteinModelPortaliP54386.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP54386.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA10756; BAA10756; BAA10756.
KEGGisyn:slr0710.
PATRICi23843116. VBISynSp132158_3096.

Phylogenomic databases

HOGENOMiHOG000243801.
InParanoidiP54386.
KOiK00262.
OMAiNEMIMGW.
PhylomeDBiP54386.

Family and domain databases

CDDicd01076. NAD_bind_1_Glu_DH. 1 hit.
Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR033524. Glu/Leu/Phe/Val_DH_AS.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
IPR033922. NAD_bind_Glu_DH.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHE4_SYNY3
AccessioniPrimary (citable) accession number: P54386
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.