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Protein

Glutamate dehydrogenase, mitochondrial

Gene

Gdh

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.PROSITE-ProRule annotation

Enzyme regulationi

Subject to allosteric regulation. Activated by ADP. Inhibited by GTP and ATP (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei138SubstrateBy similarity1
Binding sitei162SubstrateBy similarity1
Binding sitei167NADBy similarity1
Active sitei174PROSITE-ProRule annotation1
Binding sitei243NADBy similarity1
Binding sitei257GTPBy similarity1
Binding sitei261GTPBy similarity1
Binding sitei310GTPBy similarity1
Binding sitei313GTPBy similarity1
Binding sitei430SubstrateBy similarity1
Binding sitei436NADBy similarity1
Binding sitei442ADPBy similarity1
Binding sitei521ADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi132 – 134NADBy similarity3

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glutamate dehydrogenase (NAD+) activity Source: FlyBase
  • glutamate dehydrogenase [NAD(P)+] activity Source: FlyBase
  • GTP binding Source: UniProtKB-KW
  • identical protein binding Source: FlyBase

GO - Biological processi

  • cellular amino acid metabolic process Source: InterPro
  • glutamate metabolic process Source: FlyBase
  • NADH oxidation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

ATP-binding, GTP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-70614. Amino acid synthesis and interconversion (transamination).

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate dehydrogenase, mitochondrial (EC:1.4.1.3)
Short name:
GDH
Gene namesi
Name:Gdh
Synonyms:Glud
ORF Names:CG5320
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0001098. Gdh.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: FlyBase
  • cytosol Source: FlyBase
  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 53MitochondrionSequence analysisAdd BLAST53
ChainiPRO_000000721454 – 562Glutamate dehydrogenase, mitochondrialAdd BLAST509

Proteomic databases

PaxDbiP54385.
PRIDEiP54385.

Expressioni

Tissue specificityi

Expressed throughout the embryo during early stages before becoming localized in mesodermal tissue by stage 8. At stage 12 expression is concentrated in the posterior midgut. After stage 13 expression it is found in the anterior and posterior midgut, the hindgut, fat body, muscle, and central nervous system. In 3rd instar larvae expression is found in the leg disks, the wing pouch of the wing disk, the eye-antenna disk, and the developing brain medulla.1 Publication

Developmental stagei

Expressed at all stages.1 Publication

Gene expression databases

BgeeiFBgn0001098.
ExpressionAtlasiP54385. baseline.
GenevisibleiP54385. DM.

Interactioni

Subunit structurei

Homohexamer.

GO - Molecular functioni

  • identical protein binding Source: FlyBase

Protein-protein interaction databases

BioGridi67768. 23 interactors.
IntActiP54385. 5 interactors.
MINTiMINT-747345.
STRINGi7227.FBpp0088988.

Structurei

3D structure databases

ProteinModelPortaliP54385.
SMRiP54385.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2250. Eukaryota.
COG0334. LUCA.
GeneTreeiENSGT00390000000854.
InParanoidiP54385.
KOiK00261.
OMAiYMSMIGT.
OrthoDBiEOG091G0KMT.
PhylomeDBiP54385.

Family and domain databases

CDDicd01076. NAD_bind_1_Glu_DH. 1 hit.
Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR033524. Glu/Leu/Phe/Val_DH_AS.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR016040. NAD(P)-bd_dom.
IPR033922. NAD_bind_Glu_DH.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P54385-1) [UniParc]FASTAAdd to basket
Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYHLKSLARQ GARRQQELAT LAKALPTAVM QSSRGYATEH QIPDRLKDVP
60 70 80 90 100
TAKDPRFFDM VEYFFHRGCQ IAEESLVDDM KGKLTRDEKK QKVKGILMLM
110 120 130 140 150
QPCDHIIEIA FPLRRDAGNY EMITGYRAQH STHKTPTKGG IRFSLDVSRD
160 170 180 190 200
EVKALSALMT FKCACVDVPF GGAKAGLKIN PKEYSEHELE KITRRFTLEL
210 220 230 240 250
AKKGFIGPGV DVPAPDMGTG EREMSWIADT YAKTIGHLDI NAHACVTGKP
260 270 280 290 300
INQGGIHGRV SATGRGVFHG LENFINEANY MSQIGTTPGW GGKTFIVQGF
310 320 330 340 350
GNVGLHTTRY LTRAGATCIG VIEHDGTLYN PEGIDPKLLE DYKNEHGTIV
360 370 380 390 400
GYQNAKPYEG ENLMFEKCDI FIPAAVEKVI TSENANRIQA KIIAEAANGP
410 420 430 440 450
TTPAADKILI DRNILVIPDL YINAGGVTVS FFEWLKNLNH VSYGRLTFKY
460 470 480 490 500
ERESNYHLLA SVQQSIERII NDESVQESLE RRFGRVGGRI PVTPSESFQK
510 520 530 540 550
RISGASEKDI VHSGLDYTME RSARAIMKTA MKYNLGLDLR TAAYVNSIEK
560
IFTTYRDAGL AF
Length:562
Mass (Da):62,536
Last modified:December 1, 2000 - v2
Checksum:i687A183D3BC0211F
GO
Isoform C (identifier: P54385-2) [UniParc]FASTAAdd to basket
Also known as: Short, F

The sequence of this isoform differs from the canonical sequence as follows:
     460-472: Missing.

Show »
Length:549
Mass (Da):61,082
Checksum:iE4FA8AE7638654B1
GO

Sequence cautioni

The sequence AAN71256 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6 – 180Missing in AAN71256 (PubMed:12537569).CuratedAdd BLAST175
Sequence conflicti11 – 15GARRQ → APAAR in CAA72173 (PubMed:10992165).Curated5

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001285460 – 472Missing in isoform C. 1 PublicationAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11314 mRNA. Translation: CAA72173.1.
Z29062 mRNA. Translation: CAA82304.1.
AE014297 Genomic DNA. Translation: AAF56209.5.
AE014297 Genomic DNA. Translation: AAS65200.1.
AY061323 mRNA. Translation: AAL28871.1.
BT001501 mRNA. Translation: AAN71256.1. Different initiation.
PIRiS42919.
RefSeqiNP_524470.4. NM_079746.6. [P54385-1]
NP_996274.1. NM_206551.3. [P54385-2]
UniGeneiDm.3641.

Genome annotation databases

EnsemblMetazoaiFBtr0089498; FBpp0088988; FBgn0001098. [P54385-1]
GeneIDi42832.
KEGGidme:Dmel_CG5320.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11314 mRNA. Translation: CAA72173.1.
Z29062 mRNA. Translation: CAA82304.1.
AE014297 Genomic DNA. Translation: AAF56209.5.
AE014297 Genomic DNA. Translation: AAS65200.1.
AY061323 mRNA. Translation: AAL28871.1.
BT001501 mRNA. Translation: AAN71256.1. Different initiation.
PIRiS42919.
RefSeqiNP_524470.4. NM_079746.6. [P54385-1]
NP_996274.1. NM_206551.3. [P54385-2]
UniGeneiDm.3641.

3D structure databases

ProteinModelPortaliP54385.
SMRiP54385.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi67768. 23 interactors.
IntActiP54385. 5 interactors.
MINTiMINT-747345.
STRINGi7227.FBpp0088988.

Proteomic databases

PaxDbiP54385.
PRIDEiP54385.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0089498; FBpp0088988; FBgn0001098. [P54385-1]
GeneIDi42832.
KEGGidme:Dmel_CG5320.

Organism-specific databases

CTDi42832.
FlyBaseiFBgn0001098. Gdh.

Phylogenomic databases

eggNOGiKOG2250. Eukaryota.
COG0334. LUCA.
GeneTreeiENSGT00390000000854.
InParanoidiP54385.
KOiK00261.
OMAiYMSMIGT.
OrthoDBiEOG091G0KMT.
PhylomeDBiP54385.

Enzyme and pathway databases

ReactomeiR-DME-70614. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

ChiTaRSiGdh. fly.
GenomeRNAii42832.
PROiP54385.

Gene expression databases

BgeeiFBgn0001098.
ExpressionAtlasiP54385. baseline.
GenevisibleiP54385. DM.

Family and domain databases

CDDicd01076. NAD_bind_1_Glu_DH. 1 hit.
Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR033524. Glu/Leu/Phe/Val_DH_AS.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR016040. NAD(P)-bd_dom.
IPR033922. NAD_bind_Glu_DH.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHE3_DROME
AccessioniPrimary (citable) accession number: P54385
Secondary accession number(s): P91624, Q8IH05, Q9VCF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 1, 2000
Last modified: November 30, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

ADP can occupy the NADH binding site and activate the enzyme.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.