ID   ISPA_BACSU              Reviewed;         296 AA.
AC   P54383;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 2.
DT   07-JUL-2009, entry version 63.
DE   RecName: Full=Geranyltranstransferase;
DE            EC=2.5.1.10;
DE   AltName: Full=Farnesyl-diphosphate synthase;
DE            Short=FPP synthase;
GN   Name=ispA; Synonyms=yqiD; OrderedLocusNames=BSU24280;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   MEDLINE=97124195; PubMed=8969508;
RA   Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA   Kobayashi Y.;
RT   "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of
RT   the Bacillus subtilis genome containing the skin element and many
RT   sporulation genes.";
RL   Microbiology 142:3103-3111(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 166; 184 AND C-TERMINUS.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G.,
RA   Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus
RT   subtilis 168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
CC   -!- CATALYTIC ACTIVITY: Geranyl diphosphate + isopentenyl diphosphate
CC       = diphosphate + trans,trans-farnesyl diphosphate.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the FPP/GGPP synthetase family.
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DR   EMBL; D84432; BAA12575.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14359.2; -; Genomic_DNA.
DR   PIR; A69961; A69961.
DR   RefSeq; NP_390308.1; -.
DR   GeneID; 938652; -.
DR   GenomeReviews; AL009126_GR; BSU24280.
DR   KEGG; bsu:BSU24280; -.
DR   NMPDR; fig|224308.1.peg.2432; -.
DR   SubtiList; BG11714; ispA.
DR   HOGENOM; P54383; -.
DR   OMA; P54383; HANITIC.
DR   BioCyc; BSUB224308:BSU2426-MON; -.
DR   BRENDA; 2.5.1.10; 150.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004337; F:geranyltranstransferase activity; IEA:EC.
DR   GO; GO:0008299; P:isoprenoid biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR000092; Polyprenyl_synt.
DR   InterPro; IPR017446; Polyprenyl_synth-rel.
DR   InterPro; IPR008949; Terpenoid_synth.
DR   Gene3D; G3DSA:1.10.600.10; Terpenoid_synth; 1.
DR   PANTHER; PTHR12001; Polyprenyl_synt; 1.
DR   Pfam; PF00348; polyprenyl_synt; 1.
DR   PROSITE; PS00723; POLYPRENYL_SYNTHET_1; 1.
DR   PROSITE; PS00444; POLYPRENYL_SYNTHET_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Isoprene biosynthesis; Transferase.
FT   CHAIN         1    296       Geranyltranstransferase.
FT                                /FTId=PRO_0000123986.
FT   CONFLICT    166    166       E -> G (in Ref. 1; BAA12575).
FT   CONFLICT    184    184       A -> S (in Ref. 1; BAA12575).
FT   CONFLICT    271    296       KRLIGGLSLQKDLLYELCDLIAARDH -> ND (in Ref.
FT                                1; BAA12575).
SQ   SEQUENCE   296 AA;  32503 MW;  EECDBE7B483F2ECB CRC64;
     MTNKLTSFLA DRKKTIENQL SVYTEKLDMP DSLKKSMLYS LQAGGKRLRP LIVLAVLNAY
     GKSEKDGIPV GCAVEMIHTY SLIHDDLPCM DDDDLRRGKP TNHKVFGEAT AVLAGDGLLT
     ESFKLITSHV SDEVSAEKRL RLVNELISAA GTEGMVGGQV ADMEAENRQV TLEELESIHE
     RKTAKLLGFC VIAGAILADA PEEDIETLRT FSSHIGIGFQ IRDDILDLEG SEEKIGKRVG
     SDTTNDKSTY PSLLSLEGAK HKLDVHIKEA KRLIGGLSLQ KDLLYELCDL IAARDH
//