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Reviewed, UniProtKB/Swiss-Prot P54383 (ISPA_BACSU)

Last modified November 3, 2009. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Geranyltranstransferase
    EC=2.5.1.10
Alternative name(s):
    Farnesyl-diphosphate synthase
      Short name=FPP synthase
Gene names
Name: ispA
Synonyms: yqiD
Ordered Locus Names: BSU24280
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

Geranyl diphosphate + isopentenyl diphosphate = diphosphate + trans,trans-farnesyl diphosphate.

Cofactor

Binds 3 magnesium ions per subunit By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the FPP/GGPP synthetase family.

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Ontologies

Keywords
   Biological processIsoprene biosynthesis
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processisoprenoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiongeranyltranstransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 296296Geranyltranstransferase
PRO_0000123986

Sites

Metal binding851Magnesium 1 By similarity
Metal binding851Magnesium 2 By similarity
Metal binding911Magnesium 1 By similarity
Metal binding911Magnesium 2 By similarity
Metal binding2231Magnesium 3 By similarity
Binding site461Isopentenyl diphosphate By similarity
Binding site491Isopentenyl diphosphate By similarity
Binding site781Isopentenyl diphosphate By similarity
Binding site961Geranyl diphosphate By similarity
Binding site971Isopentenyl diphosphate By similarity
Binding site1821Geranyl diphosphate By similarity
Binding site1831Geranyl diphosphate By similarity
Binding site2201Geranyl diphosphate By similarity
Binding site2371Geranyl diphosphate By similarity

Experimental info

Sequence conflict1661E → G in BAA12575. Ref.1
Sequence conflict1841A → S in BAA12575. Ref.1
Sequence conflict271 – 29626KRLIG…AARDH → ND in BAA12575. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P54383-1 [UniParc].

Last modified July 7, 2009. Version 2.
Checksum: EECDBE7B483F2ECB

FASTA29632,503
        10         20         30         40         50         60 
MTNKLTSFLA DRKKTIENQL SVYTEKLDMP DSLKKSMLYS LQAGGKRLRP LIVLAVLNAY 

        70         80         90        100        110        120 
GKSEKDGIPV GCAVEMIHTY SLIHDDLPCM DDDDLRRGKP TNHKVFGEAT AVLAGDGLLT 

       130        140        150        160        170        180 
ESFKLITSHV SDEVSAEKRL RLVNELISAA GTEGMVGGQV ADMEAENRQV TLEELESIHE 

       190        200        210        220        230        240 
RKTAKLLGFC VIAGAILADA PEEDIETLRT FSSHIGIGFQ IRDDILDLEG SEEKIGKRVG 

       250        260        270        280        290 
SDTTNDKSTY PSLLSLEGAK HKLDVHIKEA KRLIGGLSLQ KDLLYELCDL IAARDH

« Hide

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References

« Hide 'large scale' references
[1]"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
Microbiology 142:3103-3111(1996) [PubMed: 8969508] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / JH642.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract]
Cited for: SEQUENCE REVISION TO 166; 184 AND C-TERMINUS.

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Cross-references

Sequence databases

D84432 Genomic DNA. Translation: BAA12575.1.
AL009126 Genomic DNA. Translation: CAB14359.2.
PIRA69961.
RefSeqNP_390308.2.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID938652.
GenomeReviewsGene locus BSU24280 in contig AL009126_GR.
KEGGbsu:BSU24280.
NMPDRfig|224308.1.peg.2432.

Organism-specific databases

SubtiListBG11714. ispA. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMP54383.
OMAHANITIC.

Enzyme and pathway databases

BioCycBSUB224308:BSU2426-MON.
BRENDA2.5.1.10. 150.

Family and domain databases

InterProIPR000092. Polyprenyl_synt.
[Graphical view]
PROSITEPS00723. POLYPRENYL_SYNTHET_1. 1 hit.
PS00444. POLYPRENYL_SYNTHET_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameISPA_BACSU
AccessionPrimary (citable) accession number: P54383
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 7, 2009
Last modified: November 3, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents