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Protein

Aminomethyltransferase

Gene

gcvT

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

The glycine cleavage system catalyzes the degradation of glycine.UniRule annotation

Catalytic activityi

[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

BioCyciBSUB:BSU24570-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
AminomethyltransferaseUniRule annotation (EC:2.1.2.10UniRule annotation)
Alternative name(s):
Glycine cleavage system T proteinUniRule annotation
Gene namesi
Name:gcvTUniRule annotation
Synonyms:yqhI
Ordered Locus Names:BSU24570
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 362362AminomethyltransferasePRO_0000122543Add
BLAST

Proteomic databases

PaxDbiP54378.

Interactioni

Subunit structurei

The glycine cleavage system is composed of four proteins: P, T, L and H.UniRule annotation

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100013466.

Structurei

Secondary structure

1
362
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 103Combined sources
Helixi11 – 144Combined sources
Beta strandi17 – 215Combined sources
Beta strandi24 – 318Combined sources
Helixi33 – 4210Combined sources
Beta strandi43 – 486Combined sources
Beta strandi52 – 598Combined sources
Helixi62 – 698Combined sources
Beta strandi70 – 723Combined sources
Helixi74 – 763Combined sources
Beta strandi81 – 888Combined sources
Beta strandi94 – 10411Combined sources
Beta strandi107 – 1126Combined sources
Helixi114 – 1163Combined sources
Helixi117 – 12610Combined sources
Beta strandi133 – 1364Combined sources
Turni138 – 1403Combined sources
Beta strandi141 – 1488Combined sources
Helixi151 – 1566Combined sources
Beta strandi159 – 1613Combined sources
Helixi163 – 1653Combined sources
Beta strandi170 – 1778Combined sources
Beta strandi180 – 1867Combined sources
Beta strandi189 – 20012Combined sources
Helixi201 – 2033Combined sources
Helixi204 – 21512Combined sources
Helixi216 – 2183Combined sources
Beta strandi220 – 2234Combined sources
Helixi225 – 23410Combined sources
Turni240 – 2423Combined sources
Beta strandi243 – 2453Combined sources
Turni250 – 2545Combined sources
Helixi256 – 2583Combined sources
Helixi270 – 27910Combined sources
Beta strandi282 – 29312Combined sources
Beta strandi301 – 3044Combined sources
Beta strandi307 – 31812Combined sources
Turni319 – 3224Combined sources
Beta strandi323 – 3319Combined sources
Helixi332 – 3343Combined sources
Beta strandi340 – 3456Combined sources
Beta strandi348 – 3558Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YX2X-ray2.08A/B1-362[»]
ProteinModelPortaliP54378.
SMRiP54378. Positions 1-362.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54378.

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvT family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D43. Bacteria.
COG0404. LUCA.
HOGENOMiHOG000239381.
InParanoidiP54378.
KOiK00605.
OMAiLGWLVHL.
OrthoDBiEOG6T1WS5.
PhylomeDBiP54378.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
HAMAPiMF_00259. GcvT.
InterProiIPR006223. GCS_T.
IPR022903. GCS_T_bac.
IPR028896. GCST/DmdA.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PANTHERiPTHR13847:SF5. PTHR13847:SF5. 1 hit.
PfamiPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006487. GcvT. 1 hit.
SUPFAMiSSF101790. SSF101790. 1 hit.
TIGRFAMsiTIGR00528. gcvT. 1 hit.

Sequencei

Sequence statusi: Complete.

P54378-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKRTPLFDL YKEYGGKTID FGGWELPVQF SSIKKEHEAV RTAAGLFDVS
60 70 80 90 100
HMGEVEVSGN DSLSFLQRLM TNDVSALTPG RAQYTAMCYP DGGTVDDLLI
110 120 130 140 150
YQKGENRYLL VINASNIDKD LAWMKEHAAG DVQIDNQSDQ IALLAVQGPK
160 170 180 190 200
AEAILKNLTD ADVSALKPFA FIDEADISGR KALISRTGYT GEDGYEIYCR
210 220 230 240 250
SDDAMHIWKK IIDAGDAYGL IPCGLGARDT LRFEAKLPLY GQELTRDITP
260 270 280 290 300
IEAGIGFAVK HKKESDFFGK SVLSEQKENG AKRKLVGLEM IEKGIPRHGY
310 320 330 340 350
EVFQNGKSVG KVTTGTQSPT LGKNVGLALI DSETSEIGTV VDVEIRKKLV
360
KAKVVKTPFY KR
Length:362
Mass (Da):39,807
Last modified:July 7, 2009 - v2
Checksum:i25D814C97FE94A1B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti236 – 2372KL → NV in BAA12546 (PubMed:8969508).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84432 Genomic DNA. Translation: BAA12546.1.
AL009126 Genomic DNA. Translation: CAB14388.2.
PIRiH69958.
RefSeqiNP_390337.2. NC_000964.3.
WP_004398598.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14388; CAB14388; BSU24570.
GeneIDi938547.
KEGGibsu:BSU24570.
PATRICi18976748. VBIBacSub10457_2564.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84432 Genomic DNA. Translation: BAA12546.1.
AL009126 Genomic DNA. Translation: CAB14388.2.
PIRiH69958.
RefSeqiNP_390337.2. NC_000964.3.
WP_004398598.1. NZ_JNCM01000036.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YX2X-ray2.08A/B1-362[»]
ProteinModelPortaliP54378.
SMRiP54378. Positions 1-362.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100013466.

Proteomic databases

PaxDbiP54378.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14388; CAB14388; BSU24570.
GeneIDi938547.
KEGGibsu:BSU24570.
PATRICi18976748. VBIBacSub10457_2564.

Phylogenomic databases

eggNOGiENOG4105D43. Bacteria.
COG0404. LUCA.
HOGENOMiHOG000239381.
InParanoidiP54378.
KOiK00605.
OMAiLGWLVHL.
OrthoDBiEOG6T1WS5.
PhylomeDBiP54378.

Enzyme and pathway databases

BioCyciBSUB:BSU24570-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP54378.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
HAMAPiMF_00259. GcvT.
InterProiIPR006223. GCS_T.
IPR022903. GCS_T_bac.
IPR028896. GCST/DmdA.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PANTHERiPTHR13847:SF5. PTHR13847:SF5. 1 hit.
PfamiPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006487. GcvT. 1 hit.
SUPFAMiSSF101790. SSF101790. 1 hit.
TIGRFAMsiTIGR00528. gcvT. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
    Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
    Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
    Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
    Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 236-237.

Entry informationi

Entry nameiGCST_BACSU
AccessioniPrimary (citable) accession number: P54378
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 7, 2009
Last modified: February 17, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.