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P54378 (GCST_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aminomethyltransferase
EC=2.1.2.10
Alternative name(s):
Glycine cleavage system T protein
Gene names
Name:gcvT
Synonyms:yqhI
Ordered Locus Names:BSU24570
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length362 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP MF_00259

Catalytic activity

[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259

Subunit structure

The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.

Sequence similarities

Belongs to the GcvT family.

Ontologies

Keywords
   Molecular functionAminotransferase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological processglycine catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: InterPro

   Molecular functionaminomethyltransferase activity

Inferred from electronic annotation. Source: EC

transaminase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 362362Aminomethyltransferase HAMAP MF_00259
PRO_0000122543

Experimental info

Sequence conflict236 – 2372KL → NV in BAA12546. Ref.1

Secondary structure

1 ......................................................................... 362
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P54378 [UniParc].

Last modified July 7, 2009. Version 2.
Checksum: 25D814C97FE94A1B

FASTA36239,807
        10         20         30         40         50         60 
MLKRTPLFDL YKEYGGKTID FGGWELPVQF SSIKKEHEAV RTAAGLFDVS HMGEVEVSGN 

        70         80         90        100        110        120 
DSLSFLQRLM TNDVSALTPG RAQYTAMCYP DGGTVDDLLI YQKGENRYLL VINASNIDKD 

       130        140        150        160        170        180 
LAWMKEHAAG DVQIDNQSDQ IALLAVQGPK AEAILKNLTD ADVSALKPFA FIDEADISGR 

       190        200        210        220        230        240 
KALISRTGYT GEDGYEIYCR SDDAMHIWKK IIDAGDAYGL IPCGLGARDT LRFEAKLPLY 

       250        260        270        280        290        300 
GQELTRDITP IEAGIGFAVK HKKESDFFGK SVLSEQKENG AKRKLVGLEM IEKGIPRHGY 

       310        320        330        340        350        360 
EVFQNGKSVG KVTTGTQSPT LGKNVGLALI DSETSEIGTV VDVEIRKKLV KAKVVKTPFY 


KR

« Hide

References

« Hide 'large scale' references
[1]"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
Microbiology 142:3103-3111(1996) [PubMed: 8969508] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract]
Cited for: SEQUENCE REVISION TO 236-237.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D84432 Genomic DNA. Translation: BAA12546.1.
AL009126 Genomic DNA. Translation: CAB14388.2.
PIRH69958.
RefSeqNP_390337.2. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1YX2X-ray2.08A/B1-362[»]
ProteinModelPortalP54378.
SMRP54378. Positions 1-362.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000001489; EBBACP00000001489; EBBACG00000001487.
GeneID938547.
GenomeReviewsGene locus BSU24570 in contig AL009126_GR.
KEGGbsu:BSU24570.
NMPDRfig|224308.1.peg.2461.
PATRIC18976748. VBIBacSub10457_2564.

Organism-specific databases

GenoListBSU24570. [Micado]

Phylogenomic databases

GeneTreeEBGT00050000001932.
HOGENOMHBG299834.
PhylomeDBP54378.
ProtClustDBPRK00389.

Enzyme and pathway databases

BioCycBSUB:BSU24570-MONOMER.

Family and domain databases

HAMAPMF_00259. GcvT.
[Tree]
InterProIPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR006223. GcvT.
IPR022903. NH2_Me_Trfase.
[Graphical view]
KOK00605.
PANTHERPTHR13847:SF5. PTHR13847:SF5. 1 hit.
PfamPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFPIRSF006487. GcvT. 1 hit.
TIGRFAMsTIGR00528. GcvT. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGCST_BACSU
AccessionPrimary (citable) accession number: P54378
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 7, 2009
Last modified: January 25, 2012
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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