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Protein

Aminomethyltransferase

Gene

gcvT

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

The glycine cleavage system catalyzes the degradation of glycine.UniRule annotation

Catalytic activityi

[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

BioCyciBSUB:BSU24570-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
AminomethyltransferaseUniRule annotation (EC:2.1.2.10UniRule annotation)
Alternative name(s):
Glycine cleavage system T proteinUniRule annotation
Gene namesi
Name:gcvTUniRule annotation
Synonyms:yqhI
Ordered Locus Names:BSU24570
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001225431 – 362AminomethyltransferaseAdd BLAST362

Proteomic databases

PaxDbiP54378.

Interactioni

Subunit structurei

The glycine cleavage system is composed of four proteins: P, T, L and H.UniRule annotation

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100013466.

Structurei

Secondary structure

1362
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 10Combined sources3
Helixi11 – 14Combined sources4
Beta strandi17 – 21Combined sources5
Beta strandi24 – 31Combined sources8
Helixi33 – 42Combined sources10
Beta strandi43 – 48Combined sources6
Beta strandi52 – 59Combined sources8
Helixi62 – 69Combined sources8
Beta strandi70 – 72Combined sources3
Helixi74 – 76Combined sources3
Beta strandi81 – 88Combined sources8
Beta strandi94 – 104Combined sources11
Beta strandi107 – 112Combined sources6
Helixi114 – 116Combined sources3
Helixi117 – 126Combined sources10
Beta strandi133 – 136Combined sources4
Turni138 – 140Combined sources3
Beta strandi141 – 148Combined sources8
Helixi151 – 156Combined sources6
Beta strandi159 – 161Combined sources3
Helixi163 – 165Combined sources3
Beta strandi170 – 177Combined sources8
Beta strandi180 – 186Combined sources7
Beta strandi189 – 200Combined sources12
Helixi201 – 203Combined sources3
Helixi204 – 215Combined sources12
Helixi216 – 218Combined sources3
Beta strandi220 – 223Combined sources4
Helixi225 – 234Combined sources10
Turni240 – 242Combined sources3
Beta strandi243 – 245Combined sources3
Turni250 – 254Combined sources5
Helixi256 – 258Combined sources3
Helixi270 – 279Combined sources10
Beta strandi282 – 293Combined sources12
Beta strandi301 – 304Combined sources4
Beta strandi307 – 318Combined sources12
Turni319 – 322Combined sources4
Beta strandi323 – 331Combined sources9
Helixi332 – 334Combined sources3
Beta strandi340 – 345Combined sources6
Beta strandi348 – 355Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YX2X-ray2.08A/B1-362[»]
ProteinModelPortaliP54378.
SMRiP54378.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54378.

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvT family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D43. Bacteria.
COG0404. LUCA.
HOGENOMiHOG000239381.
InParanoidiP54378.
KOiK00605.
OMAiLGWLVHL.
PhylomeDBiP54378.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
HAMAPiMF_00259. GcvT. 1 hit.
InterProiIPR006223. GCS_T.
IPR022903. GCS_T_bac.
IPR028896. GCST/DmdA.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PANTHERiPTHR13847:SF5. PTHR13847:SF5. 1 hit.
PfamiPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006487. GcvT. 1 hit.
SUPFAMiSSF101790. SSF101790. 1 hit.
TIGRFAMsiTIGR00528. gcvT. 1 hit.

Sequencei

Sequence statusi: Complete.

P54378-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKRTPLFDL YKEYGGKTID FGGWELPVQF SSIKKEHEAV RTAAGLFDVS
60 70 80 90 100
HMGEVEVSGN DSLSFLQRLM TNDVSALTPG RAQYTAMCYP DGGTVDDLLI
110 120 130 140 150
YQKGENRYLL VINASNIDKD LAWMKEHAAG DVQIDNQSDQ IALLAVQGPK
160 170 180 190 200
AEAILKNLTD ADVSALKPFA FIDEADISGR KALISRTGYT GEDGYEIYCR
210 220 230 240 250
SDDAMHIWKK IIDAGDAYGL IPCGLGARDT LRFEAKLPLY GQELTRDITP
260 270 280 290 300
IEAGIGFAVK HKKESDFFGK SVLSEQKENG AKRKLVGLEM IEKGIPRHGY
310 320 330 340 350
EVFQNGKSVG KVTTGTQSPT LGKNVGLALI DSETSEIGTV VDVEIRKKLV
360
KAKVVKTPFY KR
Length:362
Mass (Da):39,807
Last modified:July 7, 2009 - v2
Checksum:i25D814C97FE94A1B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti236 – 237KL → NV in BAA12546 (PubMed:8969508).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84432 Genomic DNA. Translation: BAA12546.1.
AL009126 Genomic DNA. Translation: CAB14388.2.
PIRiH69958.
RefSeqiNP_390337.2. NC_000964.3.
WP_004398598.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14388; CAB14388; BSU24570.
GeneIDi938547.
KEGGibsu:BSU24570.
PATRICi18976748. VBIBacSub10457_2564.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84432 Genomic DNA. Translation: BAA12546.1.
AL009126 Genomic DNA. Translation: CAB14388.2.
PIRiH69958.
RefSeqiNP_390337.2. NC_000964.3.
WP_004398598.1. NZ_JNCM01000036.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YX2X-ray2.08A/B1-362[»]
ProteinModelPortaliP54378.
SMRiP54378.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100013466.

Proteomic databases

PaxDbiP54378.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14388; CAB14388; BSU24570.
GeneIDi938547.
KEGGibsu:BSU24570.
PATRICi18976748. VBIBacSub10457_2564.

Phylogenomic databases

eggNOGiENOG4105D43. Bacteria.
COG0404. LUCA.
HOGENOMiHOG000239381.
InParanoidiP54378.
KOiK00605.
OMAiLGWLVHL.
PhylomeDBiP54378.

Enzyme and pathway databases

BioCyciBSUB:BSU24570-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP54378.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 2 hits.
HAMAPiMF_00259. GcvT. 1 hit.
InterProiIPR006223. GCS_T.
IPR022903. GCS_T_bac.
IPR028896. GCST/DmdA.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PANTHERiPTHR13847:SF5. PTHR13847:SF5. 1 hit.
PfamiPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006487. GcvT. 1 hit.
SUPFAMiSSF101790. SSF101790. 1 hit.
TIGRFAMsiTIGR00528. gcvT. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGCST_BACSU
AccessioniPrimary (citable) accession number: P54378
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 7, 2009
Last modified: November 2, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.