Reviewed, UniProtKB/Swiss-Prot P54378 (GCST_BACSU)

Names and origin

Protein names

Recommended name:
    Aminomethyltransferase
    EC=2.1.2.10
Alternative name(s):
    Glycine cleavage system T protein

Gene names

Name:	gcvT
Synonyms:	yqhI
Ordered Locus Names:	BSU24570

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity.
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the gcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: HAMAP transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 362

362

Aminomethyltransferase HAMAP MF_00259

PRO_0000122543

Experimental info

Sequence conflict

236 – 237

2

KL → NV in BAA12546. Ref.1

Secondary structure

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362

Helix Strand Turn

Details...

Helix

8 – 10

3

Helix

11 – 14

4

Beta strand

17 – 21

5

Beta strand

24 – 31

8

Helix

33 – 42

10

Beta strand

43 – 48

6

Beta strand

52 – 59

8

Helix

62 – 69

8

Beta strand

70 – 72

3

Helix

74 – 76

3

Beta strand

81 – 88

8

Beta strand

94 – 104

11

Beta strand

107 – 112

6

Helix

114 – 116

3

Helix

117 – 126

10

Beta strand

133 – 136

4

Turn

138 – 140

3

Beta strand

141 – 148

8

Helix

151 – 156

6

Beta strand

159 – 161

3

Helix

163 – 165

3

Beta strand

170 – 177

8

Beta strand

180 – 186

7

Beta strand

189 – 200

12

Helix

201 – 203

3

Helix

204 – 215

12

Helix

216 – 218

3

Beta strand

220 – 223

4

Helix

225 – 234

10

Turn

240 – 242

3

Beta strand

243 – 245

3

Turn

250 – 254

5

Helix

256 – 258

3

Helix

270 – 279

10

Beta strand

282 – 293

12

Beta strand

301 – 304

4

Beta strand

307 – 318

12

Turn

319 – 322

4

Beta strand

323 – 331

9

Helix

332 – 334

3

Beta strand

340 – 345

6

Beta strand

348 – 355

8

Sequences

	Sequence		Length	Mass (Da)
	P54378-1 [UniParc]. Last modified July 7, 2009. Version 2. Checksum: 25D814C97FE94A1B	FASTA	362	39,807
	10 20 30 40 50 60 MLKRTPLFDL YKEYGGKTID FGGWELPVQF SSIKKEHEAV RTAAGLFDVS HMGEVEVSGN 70 80 90 100 110 120 DSLSFLQRLM TNDVSALTPG RAQYTAMCYP DGGTVDDLLI YQKGENRYLL VINASNIDKD 130 140 150 160 170 180 LAWMKEHAAG DVQIDNQSDQ IALLAVQGPK AEAILKNLTD ADVSALKPFA FIDEADISGR 190 200 210 220 230 240 KALISRTGYT GEDGYEIYCR SDDAMHIWKK IIDAGDAYGL IPCGLGARDT LRFEAKLPLY 250 260 270 280 290 300 GQELTRDITP IEAGIGFAVK HKKESDFFGK SVLSEQKENG AKRKLVGLEM IEKGIPRHGY 310 320 330 340 350 360 EVFQNGKSVG KVTTGTQSPT LGKNVGLALI DSETSEIGTV VDVEIRKKLV KAKVVKTPFY KR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes." Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y. Microbiology 142:3103-3111(1996) [PubMed: 8969508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 236-237.

Cross-references

Sequence databases

EMBLGenBankDDBJ

D84432 Genomic DNA. Translation: BAA12546.1.
AL009126 Genomic DNA. Translation: CAB14388.2.

PIR

H69958.

RefSeq

NP_390337.2.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YX2	X-ray	2.08	A/B	1-362	[»]

ModBase

Search...

Genome annotation databases

GeneID

938547.

GenomeReviews

Gene locus BSU24570 in contig AL009126_GR.

KEGG

bsu:BSU24570.

NMPDR

fig|224308.1.peg.2461.

Organism-specific databases

SubtiList

BG11509. gcvT. [Micado]

CMR

Search...

Phylogenomic databases

HOGENOM

P54378.

OMA

VEIRGKW.

Enzyme and pathway databases

BioCyc

BSUB224308:BSU2455-MON.

BRENDA

2.1.2.10. 150.

Family and domain databases

HAMAP

MF_00259.
[Tree]

InterPro

IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR006223. GcvT.
[Graphical view]

Pfam

PF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]

PIRSF

PIRSF006487. GcvT. 1 hit.

TIGRFAMs

TIGR00528. gcvT. 1 hit.

ProtoNet

Search...

Entry information

Entry name

GCST_BACSU

Accession

Primary (citable) accession number: P54378

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	July 7, 2009
Last modified:	November 3, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)