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P54376 (GCSPA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable glycine dehydrogenase (decarboxylating) subunit 1

EC=1.4.4.2
Alternative name(s):
Glycine cleavage system P-protein subunit 1
Glycine decarboxylase subunit 1
Glycine dehydrogenase (aminomethyl-transferring) subunit 1
Gene names
Name:gcvPA
Synonyms:yqhJ
Ordered Locus Names:BSU24560
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length448 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein By similarity. HAMAP-Rule MF_00712

Catalytic activity

Glycine + [glycine-cleavage complex H protein]-N(6)-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N(6)-dihydrolipoyl-L-lysine + CO2. HAMAP-Rule MF_00712

Subunit structure

The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits.

Sequence similarities

Belongs to the GcvP family. N-terminal subunit subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 448448Probable glycine dehydrogenase (decarboxylating) subunit 1 HAMAP-Rule MF_00712
PRO_0000166961

Experimental info

Sequence conflict265 – 2662QP → HR in BAA12547. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P54376 [UniParc].

Last modified July 7, 2009. Version 2.
Checksum: 441875530A324E3F

FASTA44849,429
        10         20         30         40         50         60 
MKHRYLPATE KDKQEMLATI GVSSIDDLFA DIPENVKYKK EHQIKKAKSE TELTRELTKL 

        70         80         90        100        110        120 
ASKNRDTVQY ASFLGAGVYD HYQPVIVDHV ISRSEFYTAY TPYQPEISQG ELQAIFEFQT 

       130        140        150        160        170        180 
MICELTGMDI ANSSMYDGGT ALAEAAMLAS GHTKKKKIVV SKTVHPESRE VLKTYAKGQY 

       190        200        210        220        230        240 
IDVVEVPAAD GVTDLDALRQ TVCENTAAVI VQYPNFFGRI EPLKDIEPIA HQGKSMFIVS 

       250        260        270        280        290        300 
ANPLALGLLT PPGKFQSDIV VGDAQPFGIP SAYGGPHCGF FAVTKKLMRK VPGRLVGQTE 

       310        320        330        340        350        360 
DENGKRGFVL TLQAREQHIR RDKATSNICS NQALNALAAS VAMTALGKNG VKDIARQNLL 

       370        380        390        400        410        420 
KANYAKQEAK KAGLTVMFDG PMFNEFVIKL DEPVRAVNKR LLAKGMIGGY DLGLTYPELD 

       430        440 
CHMLIAVTEL RTKEEIDALI QELGDRHE 

« Hide

References

« Hide 'large scale' references
[1]"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / JH642.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 265-266.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D84432 Genomic DNA. Translation: BAA12547.1.
AL009126 Genomic DNA. Translation: CAB14387.2.
PIRA69959.
RefSeqNP_390336.2. NC_000964.3.

3D structure databases

ProteinModelPortalP54376.
SMRP54376. Positions 4-446.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU24560.

Proteomic databases

PaxDbP54376.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB14387; CAB14387; BSU24560.
GeneID938546.
KEGGbsu:BSU24560.
PATRIC18976746. VBIBacSub10457_2563.

Organism-specific databases

GenoListBSU24560. [Micado]

Phylogenomic databases

eggNOGCOG0403.
HOGENOMHOG000132025.
KOK00282.
OrthoDBEOG6XWV3B.
ProtClustDBPRK00451.

Enzyme and pathway databases

BioCycBSUB:BSU24560-MONOMER.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
HAMAPMF_00712. GcvPA.
InterProIPR020580. GDC-P_N.
IPR020581. GDC_P.
IPR023010. GDC_P_su1.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERPTHR11773. PTHR11773. 1 hit.
PfamPF02347. GDC-P. 1 hit.
[Graphical view]
PIRSFPIRSF006815. GcvPA. 1 hit.
SUPFAMSSF53383. SSF53383. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGCSPA_BACSU
AccessionPrimary (citable) accession number: P54376
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 7, 2009
Last modified: February 19, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList