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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Protein AroA(G) (aroA)
  2. 3-dehydroquinate synthase (aroB), 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD), 3-dehydroquinate dehydratase (yqhS)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroF), Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei62 – 621ShikimateUniRule annotation
Active sitei66 – 661Proton acceptorUniRule annotation
Binding sitei78 – 781NADPUniRule annotation
Binding sitei87 – 871ShikimateUniRule annotation
Binding sitei102 – 1021ShikimateUniRule annotation
Binding sitei219 – 2191NADP; via carbonyl oxygenUniRule annotation
Binding sitei221 – 2211ShikimateUniRule annotation
Binding sitei242 – 2421NADP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi127 – 1315NADPUniRule annotation
Nucleotide bindingi151 – 1566NADPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciBSUB:BSU25660-MONOMER.
UniPathwayiUPA00053; UER00087.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Synonyms:aroD
Ordered Locus Names:BSU25660
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 280280Shikimate dehydrogenase (NADP(+))PRO_0000135992Add
BLAST

Proteomic databases

PaxDbiP54374.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100014031.

Structurei

3D structure databases

ProteinModelPortaliP54374.
SMRiP54374. Positions 1-276.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni15 – 173Shikimate bindingUniRule annotation

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X. Bacteria.
COG0169. LUCA.
HOGENOMiHOG000237875.
InParanoidiP54374.
KOiK00014.
OMAiTTVDGAW.
PhylomeDBiP54374.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.

Sequencei

Sequence statusi: Complete.

P54374-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLYGVIGN PIGHSMSPDI HNASLKDLGL DGHYHAFKVE ENDLEDAVKG
60 70 80 90 100
IRALGVQGIN VTVPHKVSIM DYLDHIDESA KVLGAVNTVR REGDKLVGYN
110 120 130 140 150
TDGEGFVKSL MKVLDKPISE LSFLMIGAGG AARAIFTTIV RNTPKKFDIC
160 170 180 190 200
NRTLEKAKRL TEATPSFHNK EVLSIKEAEE RLEQYDVIIH TTSVGMYPNV
210 220 230 240 250
DDVPLSLQRA ASSAVVCDIV YNPIQTALLK EASQKGLKTL DGVGMFVEQA
260 270 280
ALSFQLWTGQ EPNIEKMRSI VIGKLGGTEC
Length:280
Mass (Da):30,608
Last modified:July 7, 2009 - v2
Checksum:i8530CE97F50D7DE3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti139 – 1391I → F in BAA12445 (PubMed:8969508).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84432 Genomic DNA. Translation: BAA12445.1.
AL009126 Genomic DNA. Translation: CAB14508.2.
PIRiA69590.
RefSeqiNP_390444.2. NC_000964.3.
WP_003229967.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14508; CAB14508; BSU25660.
GeneIDi937820.
KEGGibsu:BSU25660.
PATRICi18976974. VBIBacSub10457_2676.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84432 Genomic DNA. Translation: BAA12445.1.
AL009126 Genomic DNA. Translation: CAB14508.2.
PIRiA69590.
RefSeqiNP_390444.2. NC_000964.3.
WP_003229967.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliP54374.
SMRiP54374. Positions 1-276.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100014031.

Proteomic databases

PaxDbiP54374.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14508; CAB14508; BSU25660.
GeneIDi937820.
KEGGibsu:BSU25660.
PATRICi18976974. VBIBacSub10457_2676.

Phylogenomic databases

eggNOGiENOG4105E2X. Bacteria.
COG0169. LUCA.
HOGENOMiHOG000237875.
InParanoidiP54374.
KOiK00014.
OMAiTTVDGAW.
PhylomeDBiP54374.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.
BioCyciBSUB:BSU25660-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAROE_BACSU
AccessioniPrimary (citable) accession number: P54374
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 7, 2009
Last modified: September 7, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.