Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Protein AroA(G) (aroA)
  2. 3-dehydroquinate synthase (aroB), 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (yqhS), 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroF), Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei62ShikimateUniRule annotation1
Active sitei66Proton acceptorUniRule annotation1
Binding sitei78NADPUniRule annotation1
Binding sitei87ShikimateUniRule annotation1
Binding sitei102ShikimateUniRule annotation1
Binding sitei219NADP; via carbonyl oxygenUniRule annotation1
Binding sitei221ShikimateUniRule annotation1
Binding sitei242NADP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi127 – 131NADPUniRule annotation5
Nucleotide bindingi151 – 156NADPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciBSUB:BSU25660-MONOMER
UniPathwayiUPA00053; UER00087

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Synonyms:aroD
Ordered Locus Names:BSU25660
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001359921 – 280Shikimate dehydrogenase (NADP(+))Add BLAST280

Proteomic databases

PaxDbiP54374
PRIDEiP54374

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100014031

Structurei

3D structure databases

ProteinModelPortaliP54374
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni15 – 17Shikimate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X Bacteria
COG0169 LUCA
HOGENOMiHOG000237875
InParanoidiP54374
KOiK00014
OMAiFGNPIKH
PhylomeDBiP54374

Family and domain databases

HAMAPiMF_00222 Shikimate_DH_AroE, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR011342 Shikimate_DH
IPR013708 Shikimate_DH-bd_N
IPR022893 Shikimate_DH_fam
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF01488 Shikimate_DH, 1 hit
PF08501 Shikimate_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00507 aroE, 1 hit

Sequencei

Sequence statusi: Complete.

P54374-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLYGVIGN PIGHSMSPDI HNASLKDLGL DGHYHAFKVE ENDLEDAVKG
60 70 80 90 100
IRALGVQGIN VTVPHKVSIM DYLDHIDESA KVLGAVNTVR REGDKLVGYN
110 120 130 140 150
TDGEGFVKSL MKVLDKPISE LSFLMIGAGG AARAIFTTIV RNTPKKFDIC
160 170 180 190 200
NRTLEKAKRL TEATPSFHNK EVLSIKEAEE RLEQYDVIIH TTSVGMYPNV
210 220 230 240 250
DDVPLSLQRA ASSAVVCDIV YNPIQTALLK EASQKGLKTL DGVGMFVEQA
260 270 280
ALSFQLWTGQ EPNIEKMRSI VIGKLGGTEC
Length:280
Mass (Da):30,608
Last modified:July 7, 2009 - v2
Checksum:i8530CE97F50D7DE3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti139I → F in BAA12445 (PubMed:8969508).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84432 Genomic DNA Translation: BAA12445.1
AL009126 Genomic DNA Translation: CAB14508.2
PIRiA69590
RefSeqiNP_390444.2, NC_000964.3
WP_003229967.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB14508; CAB14508; BSU25660
GeneIDi937820
KEGGibsu:BSU25660
PATRICifig|224308.179.peg.2789

Entry informationi

Entry nameiAROE_BACSU
AccessioniPrimary (citable) accession number: P54374
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 7, 2009
Last modified: May 23, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health