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Protein

Ornithine decarboxylase antizyme 1

Gene

Oaz1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Ornithine decarboxylase (ODC) antizyme protein that negatively regulates ODC activity and intracellular polyamine biosynthesis and uptake in response to increased intracellular polyamine levels. Binds to ODC monomers, inhibiting the assembly of the functional ODC homodimer, and targets the monomers for ubiquitin-independent proteolytic destruction by the 26S proteasome. Triggers ODC degradation by inducing the exposure of a cryptic proteasome-interacting surface of ODC. Stabilizes AZIN2 by interfering with its ubiquitination (By similarity). Also inhibits cellular uptake of polyamines by inactivating the polyamine uptake transporter (PubMed:8166639). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. Involved in the translocation of AZIN2 from ER-Golgi intermediate compartment (ERGIC) to the cytosol (By similarity).By similarity1 Publication

GO - Molecular functioni

  • enzyme binding Source: RGD
  • ornithine decarboxylase inhibitor activity Source: RGD
  • protein heterodimerization activity Source: RGD

GO - Biological processi

Keywordsi

Biological processPolyamine biosynthesis, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine decarboxylase antizyme 1
Short name:
ODC-Az
Gene namesi
Name:Oaz1
Synonyms:Oaz
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3219. Oaz1.

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002208522 – 227Ornithine decarboxylase antizyme 1Add BLAST226

Proteomic databases

PaxDbiP54370.
PRIDEiP54370.

Expressioni

Inductioni

Induced by a ribosomal frameshifting mechanism in response to increased levels of intracellular polyamines.1 Publication

Gene expression databases

BgeeiENSRNOG00000019459.

Interactioni

Subunit structurei

Interacts with ODC1 and thereby sterically blocks ODC homodimerization. Forms a ternary complex with PSMB4 and OAZ1 before PSMB4 is incorporated into the 20S proteasome. Interacts with AZIN2; this interaction disrupts the interaction between the antizyme and ODC1.By similarity

GO - Molecular functioni

  • enzyme binding Source: RGD
  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

BioGridi247534. 1 interactor.
STRINGi10116.ENSRNOP00000026341.

Structurei

Secondary structure

1227
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi95 – 97Combined sources3
Beta strandi99 – 106Combined sources8
Beta strandi114 – 120Combined sources7
Beta strandi128 – 135Combined sources8
Beta strandi138 – 142Combined sources5
Helixi154 – 167Combined sources14
Beta strandi172 – 176Combined sources5
Helixi183 – 190Combined sources8
Turni191 – 193Combined sources3
Beta strandi196 – 198Combined sources3
Beta strandi212 – 216Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZO0NMR-A94-219[»]
ProteinModelPortaliP54370.
SMRiP54370.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54370.

Family & Domainsi

Sequence similaritiesi

Belongs to the ODC antizyme family.Curated

Phylogenomic databases

eggNOGiKOG4387. Eukaryota.
ENOG4112791. LUCA.
HOGENOMiHOG000115253.
HOVERGENiHBG007857.
InParanoidiP54370.
KOiK16548.
PhylomeDBiP54370.

Family and domain databases

InterProiView protein in InterPro
IPR016181. Acyl_CoA_acyltransferase.
IPR029914. ODC-AZ_1.
IPR002993. ODC_AZ.
PANTHERiPTHR10279. PTHR10279. 1 hit.
PTHR10279:SF15. PTHR10279:SF15. 1 hit.
PfamiView protein in Pfam
PF02100. ODC_AZ. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiView protein in PROSITE
PS01337. ODC_AZ. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by ribosomal frameshifting. AlignAdd to basket

Note: A ribosomal frameshift occurs between the codons for Ser-68 and Asp-69. An autoregulatory mechanism enables modulation of frameshifting according to the cellular concentration of polyamines.1 Publication
Isoform 1 (identifier: P54370-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVKSSLQRIL NSHCFAREKE GDKRSATLHA SRTMPLLSQH SRGGCSSESS
60 70 80 90 100
RVALHCCSNL GPGPRWCSDV PHPPLKIPGG RGNSQRDHSL SASILYSDER
110 120 130 140 150
LNVTEEPTSN DKTRVLSIQC TLTEAKQVTW RAVWNGGGLY IELPAGPLPE
160 170 180 190 200
GSKDSFAALL EFAEEQLRAD HVFICFPKNR EDRAALLRTF SFLGFEIVRP
210 220
GHPLVPKRPD ACFMVYTLER EDPGEED
Length:227
Mass (Da):25,228
Last modified:January 23, 2007 - v3
Checksum:iEA28FC118ACF41D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10706 mRNA. Translation: BAA01549.1.
D10706 mRNA. Translation: BAA01550.1.
D10706 mRNA. Translation: BAA01551.1.
PIRiA55472.
RefSeqiNP_620781.1. NM_139081.2. [P54370-1]
UniGeneiRn.266.

Genome annotation databases

GeneIDi25502.
KEGGirno:25502.
UCSCiRGD:3219. rat. [P54370-1]

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10706 mRNA. Translation: BAA01549.1.
D10706 mRNA. Translation: BAA01550.1.
D10706 mRNA. Translation: BAA01551.1.
PIRiA55472.
RefSeqiNP_620781.1. NM_139081.2. [P54370-1]
UniGeneiRn.266.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZO0NMR-A94-219[»]
ProteinModelPortaliP54370.
SMRiP54370.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247534. 1 interactor.
STRINGi10116.ENSRNOP00000026341.

Proteomic databases

PaxDbiP54370.
PRIDEiP54370.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25502.
KEGGirno:25502.
UCSCiRGD:3219. rat. [P54370-1]

Organism-specific databases

CTDi4946.
RGDi3219. Oaz1.

Phylogenomic databases

eggNOGiKOG4387. Eukaryota.
ENOG4112791. LUCA.
HOGENOMiHOG000115253.
HOVERGENiHBG007857.
InParanoidiP54370.
KOiK16548.
PhylomeDBiP54370.

Miscellaneous databases

EvolutionaryTraceiP54370.
PROiP54370.

Gene expression databases

BgeeiENSRNOG00000019459.

Family and domain databases

InterProiView protein in InterPro
IPR016181. Acyl_CoA_acyltransferase.
IPR029914. ODC-AZ_1.
IPR002993. ODC_AZ.
PANTHERiPTHR10279. PTHR10279. 1 hit.
PTHR10279:SF15. PTHR10279:SF15. 1 hit.
PfamiView protein in Pfam
PF02100. ODC_AZ. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiView protein in PROSITE
PS01337. ODC_AZ. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiOAZ1_RAT
AccessioniPrimary (citable) accession number: P54370
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: February 15, 2017
This is version 114 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.