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Protein

Casein kinase I isoform alpha

Gene

CkIalpha

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Negative regulator of wg signaling (PubMed:22095083). Phosphorylates arm directly or indirectly and stimulates its degradation which prevents inappropriate wg signaling (PubMed:11955436, PubMed:11927557, PubMed:22095083). Phosphorylates smo which promotes its accumulation at the cell surface and its signaling activity in response to hh (PubMed:15616566). Together with dco, regulates proteolytic processing of ci by phosphorylating it which promotes its binding to slmb, the F-box recognition component of the SCF(slmb) E3 ubiquitin-protein ligase required for ci processing (PubMed:16326393). Inhibits condensin II interphase activity by promoting degradation of the Cap-H2 regulatory subunit and limiting the levels of chromatin-bound Cap-H2 which regulates interphase chromosome organization (PubMed:25723539).7 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+1 Publication

Enzyme regulationi

Activity increases following DNA damage.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei49ATPPROSITE-ProRule annotation1
Active sitei139Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 34ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell morphogenesis Source: FlyBase
  • circadian rhythm Source: Reactome
  • DNA repair Source: FlyBase
  • glucose homeostasis Source: FlyBase
  • negative regulation of canonical Wnt signaling pathway Source: FlyBase
  • negative regulation of smoothened signaling pathway Source: FlyBase
  • peptidyl-serine phosphorylation Source: GO_Central
  • positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: FlyBase
  • positive regulation of protein catabolic process Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of cell shape Source: GO_Central
  • Wnt signaling pathway Source: UniProtKB-KW

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processWnt signaling pathway
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1 1994
ReactomeiR-DME-195253 Degradation of beta-catenin by the destruction complex
R-DME-196299 Beta-catenin phosphorylation cascade
R-DME-209155 Phosphorylation of AXN and APC
R-DME-209159 Assembly of the CI containing complexes
R-DME-209190 Phosphorylation of CI
R-DME-209214 Phosphorylation of SMO
R-DME-209360 Ubiquitination and proteolysis of phosphorylated CI
R-DME-209396 Phosphorylation of ARM
R-DME-209413 Assembly of the 'destruction complex'
R-DME-209440 Recruitment of the 'destruction complex' to the receptor complex, the degradation of AXN and release of ARM
R-DME-209461 Ubiquitination and degradation of phosphorylated ARM
R-DME-432553 Phosphorylation of PER and TIM
R-DME-5610785 GLI3 is processed to GLI3R by the proteasome
R-DME-5635838 Activation of SMO
SignaLinkiP54367

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase I isoform alpha (EC:2.7.11.1)
Short name:
CKI-alpha
Short name:
DmCK1
Gene namesi
Name:CkIalpha
Synonyms:CKI
ORF Names:CG2028
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0015024 CkIalpha

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi43G → D in 8B12; death primarily during embryogenesis and early larval stages with no obvious cuticle patterning defects. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001928481 – 337Casein kinase I isoform alphaAdd BLAST337

Post-translational modificationi

Phosphorylated. The dephosphorylated kinase is active in the cytoplasm while the active kinase in the nucleus is phosphorylated.1 Publication

Proteomic databases

PaxDbiP54367
PRIDEiP54367

Expressioni

Developmental stagei

Expressed during early embryogenesis and in adult females (at protein level).1 Publication

Gene expression databases

BgeeiFBgn0015024
ExpressionAtlasiP54367 differential
GenevisibleiP54367 DM

Interactioni

Subunit structurei

Interacts with cos.1 Publication

Protein-protein interaction databases

BioGridi58615, 10 interactors
DIPiDIP-22041N
IntActiP54367, 6 interactors
STRINGi7227.FBpp0073513

Structurei

3D structure databases

ProteinModelPortaliP54367
SMRiP54367
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 288Protein kinasePROSITE-ProRule annotationAdd BLAST269

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1164 Eukaryota
ENOG410XPGP LUCA
GeneTreeiENSGT00760000119040
HOGENOMiHOG000148802
InParanoidiP54367
KOiK08957
OMAiGPDYMYL
OrthoDBiEOG091G0AFG
PhylomeDBiP54367

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P54367-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKMRILKES RPEIIVGGKY RVIRKIGSGS FGDIYLGMSI QSGEEVAIKM
60 70 80 90 100
ESAHARHPQL LYEAKLYRIL SGGVGFPRIR HHGKEKNFNT LVMDLLGPSL
110 120 130 140 150
EDLFNFCTRH FTIKTVLMLV DQMIGRLEYI HLKCFIHRDI KPDNFLMGIG
160 170 180 190 200
RHCNKLFLID FGLAKKFRDP HTRHHIVYRE DKNLTGTARY ASINAHLGIE
210 220 230 240 250
QSRRDDMESL GYVMMYFNRG VLPWQGMKAN TKQQKYEKIS EKKMSTPIEV
260 270 280 290 300
LCKGSPAEFS MYLNYCRSLR FEEQPDYMYL RQLFRILFRT LNHQYDYIYD
310 320 330
WTMLKQKTHQ GQPNPAILLE QLDKDKEKQN GKPLIAD
Length:337
Mass (Da):39,535
Last modified:October 1, 1996 - v1
Checksum:i495144945093E69D
GO

Sequence cautioni

The sequence CAA64358 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti331 – 337GKPLIAD → ASP in CAA64358 (PubMed:8832407).Curated7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55848 mRNA Translation: AAB16904.1
X94695 mRNA Translation: CAA64358.1 Different initiation.
AE014298 Genomic DNA Translation: AAF48192.1
AE014298 Genomic DNA Translation: AAN09313.1
AY069346 mRNA Translation: AAL39491.1
RefSeqiNP_001162737.1, NM_001169266.3
NP_001285163.1, NM_001298234.1
NP_001285164.1, NM_001298235.1
NP_511140.1, NM_078585.6
NP_727631.1, NM_167331.4
NP_727632.1, NM_167332.3
UniGeneiDm.2166

Genome annotation databases

EnsemblMetazoaiFBtr0073680; FBpp0073513; FBgn0015024
FBtr0073682; FBpp0073515; FBgn0015024
FBtr0300380; FBpp0289609; FBgn0015024
FBtr0340408; FBpp0309354; FBgn0015024
FBtr0345162; FBpp0311372; FBgn0015024
FBtr0345163; FBpp0311373; FBgn0015024
GeneIDi32221
KEGGidme:Dmel_CG2028
UCSCiCG2028-RC d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiKC1A_DROME
AccessioniPrimary (citable) accession number: P54367
Secondary accession number(s): A4V4D3, Q0KHT2, Q9VYK2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 167 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health