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Protein

Casein kinase I isoform alpha

Gene

CkIalpha

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Negative regulator of wg signaling (PubMed:22095083). Phosphorylates arm directly or indirectly and stimulates its degradation which prevents inappropriate wg signaling (PubMed:11955436, PubMed:11927557, PubMed:22095083). Phosphorylates smo which promotes its accumulation at the cell surface and its signaling activity in response to hh (PubMed:15616566). Together with dco, regulates proteolytic processing of ci by phosphorylating it which promotes its binding to slmb, the F-box recognition component of the SCF(slmb) E3 ubiquitin-protein ligase required for ci processing (PubMed:16326393). Inhibits condensin II interphase activity by promoting degradation of the Cap-H2 regulatory subunit and limiting the levels of chromatin-bound Cap-H2 which regulates interphase chromosome organization (PubMed:25723539).7 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+1 Publication

Enzyme regulationi

Activity increases following DNA damage.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei49ATPPROSITE-ProRule annotation1
Active sitei139Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 34ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: FlyBase
  • protein serine/threonine kinase activity Source: FlyBase

GO - Biological processi

  • cell morphogenesis Source: FlyBase
  • DNA repair Source: FlyBase
  • inter-male aggressive behavior Source: FlyBase
  • negative regulation of canonical Wnt signaling pathway Source: ParkinsonsUK-UCL
  • negative regulation of smoothened signaling pathway Source: FlyBase
  • peptidyl-serine phosphorylation Source: GO_Central
  • phagocytosis Source: FlyBase
  • positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: FlyBase
  • positive regulation of protein catabolic process Source: FlyBase
  • protein catabolic process Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of cell shape Source: GO_Central
  • regulation of glucose metabolic process Source: FlyBase
  • regulation of proteolysis Source: FlyBase
  • regulation of Wnt signaling pathway Source: FlyBase
  • Wnt signaling pathway Source: UniProtKB-KW

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processWnt signaling pathway
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 1994.
ReactomeiR-DME-195253. Degradation of beta-catenin by the destruction complex.
R-DME-196299. Beta-catenin phosphorylation cascade.
R-DME-5610785. GLI3 is processed to GLI3R by the proteasome.
R-DME-5635838. Activation of SMO.
SignaLinkiP54367.

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase I isoform alpha (EC:2.7.11.1)
Short name:
CKI-alpha
Short name:
DmCK1
Gene namesi
Name:CkIalpha
Synonyms:CKI
ORF Names:CG2028
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0015024. CkIalpha.

Subcellular locationi

  • Cytoplasm 2 Publications
  • Nucleus 2 Publications

  • Note: Levels in the nucleus are significantly increased after DNA damage.1 Publication

GO - Cellular componenti

  • beta-catenin destruction complex Source: ParkinsonsUK-UCL
  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi43G → D in 8B12; death primarily during embryogenesis and early larval stages with no obvious cuticle patterning defects. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001928481 – 337Casein kinase I isoform alphaAdd BLAST337

Post-translational modificationi

Phosphorylated. The dephosphorylated kinase is active in the cytoplasm while the active kinase in the nucleus is phosphorylated.1 Publication

Proteomic databases

PaxDbiP54367.
PRIDEiP54367.

PTM databases

iPTMnetiP54367.

Expressioni

Developmental stagei

Expressed during early embryogenesis and in adult females (at protein level).1 Publication

Gene expression databases

BgeeiFBgn0015024.
ExpressionAtlasiP54367. differential.
GenevisibleiP54367. DM.

Interactioni

Subunit structurei

Interacts with cos.1 Publication

Protein-protein interaction databases

BioGridi58615. 4 interactors.
DIPiDIP-22041N.
IntActiP54367. 1 interactor.
MINTiMINT-1722510.
STRINGi7227.FBpp0073513.

Structurei

3D structure databases

ProteinModelPortaliP54367.
SMRiP54367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 288Protein kinasePROSITE-ProRule annotationAdd BLAST269

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1164. Eukaryota.
ENOG410XPGP. LUCA.
HOGENOMiHOG000148802.
InParanoidiP54367.
KOiK08957.
OMAiGPDYMYL.
OrthoDBiEOG091G0AFG.
PhylomeDBiP54367.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

P54367-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKMRILKES RPEIIVGGKY RVIRKIGSGS FGDIYLGMSI QSGEEVAIKM
60 70 80 90 100
ESAHARHPQL LYEAKLYRIL SGGVGFPRIR HHGKEKNFNT LVMDLLGPSL
110 120 130 140 150
EDLFNFCTRH FTIKTVLMLV DQMIGRLEYI HLKCFIHRDI KPDNFLMGIG
160 170 180 190 200
RHCNKLFLID FGLAKKFRDP HTRHHIVYRE DKNLTGTARY ASINAHLGIE
210 220 230 240 250
QSRRDDMESL GYVMMYFNRG VLPWQGMKAN TKQQKYEKIS EKKMSTPIEV
260 270 280 290 300
LCKGSPAEFS MYLNYCRSLR FEEQPDYMYL RQLFRILFRT LNHQYDYIYD
310 320 330
WTMLKQKTHQ GQPNPAILLE QLDKDKEKQN GKPLIAD
Length:337
Mass (Da):39,535
Last modified:October 1, 1996 - v1
Checksum:i495144945093E69D
GO

Sequence cautioni

The sequence CAA64358 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti331 – 337GKPLIAD → ASP in CAA64358 (PubMed:8832407).Curated7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55848 mRNA. Translation: AAB16904.1.
X94695 mRNA. Translation: CAA64358.1. Different initiation.
AE014298 Genomic DNA. Translation: AAF48192.1.
AE014298 Genomic DNA. Translation: AAN09313.1.
AY069346 mRNA. Translation: AAL39491.1.
RefSeqiNP_001162737.1. NM_001169266.3.
NP_001285163.1. NM_001298234.1.
NP_001285164.1. NM_001298235.1.
NP_511140.1. NM_078585.6.
NP_727631.1. NM_167331.4.
NP_727632.1. NM_167332.3.
UniGeneiDm.2166.

Genome annotation databases

EnsemblMetazoaiFBtr0073680; FBpp0073513; FBgn0015024.
FBtr0073682; FBpp0073515; FBgn0015024.
FBtr0300380; FBpp0289609; FBgn0015024.
FBtr0340408; FBpp0309354; FBgn0015024.
FBtr0345162; FBpp0311372; FBgn0015024.
FBtr0345163; FBpp0311373; FBgn0015024.
GeneIDi32221.
KEGGidme:Dmel_CG2028.
UCSCiCG2028-RC. d. melanogaster.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiKC1A_DROME
AccessioniPrimary (citable) accession number: P54367
Secondary accession number(s): A4V4D3, Q0KHT2, Q9VYK2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 5, 2017
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families