P54360 (FOJO_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein four-jointed

Cleaved into the following chain:

1. Protein four-jointed, secreted isoform

Gene names

Name:	fj
ORF Names:	CG10917

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for intermediate growth in the proximal-distal axis. Acts in ommatidial polarity determination as a secondary signal downstream of Notch, JAK/STAT and wingless. Also necessary for the initiation, up-regulation or maintenance of Notch ligand, Serrate (Ser) expression in legs, thereby participating in a feedback loop with N signaling. Sufficient for joint formation and growth in the leg. Ref.1 Ref.2 Ref.6 Ref.7 Ref.8
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein. Note: Golgi apparatus, in the wing pouch. Ref.7 Ref.8 Protein four-jointed, secreted isoform: Secreted Ref.7 Ref.8.
Tissue specificity	In the eye disk, expressed in a gradient ahead of the morphogenetic furrow, high at the equator and low at the poles of the eye. In the leg disk, expressed in concentric rings, possibly corresponding to segmental boundaries. In the wing disk, expression is localized in the wing pouch; low in peripheral regions and high towards the center. Ref.1 Ref.2 Ref.6 Ref.7 Ref.8
Post-translational modification	Proteolytically cleaved to yield a secreted protein. Ref.8
Disruption phenotype	Mutants show reduced growth and altered differentiation only within restricted sectors of the proximal-distal (PD) axis in the leg and wing. Ref.1
Sequence similarities	Belongs to the FJX1/FJ family.

Ontologies

Keywords
Biological process	Notch signaling pathway Wnt signaling pathway
Cellular component	Golgi apparatus Membrane Secreted
Domain	Signal-anchor Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Notch signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW Wnt receptor signaling pathway, planar cell polarity pathway Inferred from genetic interaction. Source: FlyBase cell-cell signaling Inferred from direct assay Ref.1. Source: FlyBase establishment of epithelial cell apical/basal polarity Inferred from mutant phenotype. Source: FlyBase establishment of imaginal disc-derived wing hair orientation Inferred from mutant phenotype. Source: FlyBase establishment of ommatidial planar polarity Traceable author statement. Source: FlyBase imaginal disc growth Inferred from mutant phenotype Ref.1. Source: FlyBase imaginal disc-derived leg joint morphogenesis Inferred from mutant phenotype Ref.7. Source: FlyBase imaginal disc-derived wing vein specification Inferred from mutant phenotype. Source: FlyBase regulation of tube length, open tracheal system Inferred from mutant phenotype. Source: FlyBase
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay Ref.1. Source: FlyBase
Molecular function	Wnt-protein binding Inferred from physical interaction. Source: FlyBase protein kinase activity Inferred from direct assay. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 583	583	Protein four-jointed		PRO_0000087324
	Chain	? – 583		Protein four-jointed, secreted isoform		PRO_0000292347
Regions
	Topological domain	1 – 78	78	Cytoplasmic Potential
	Transmembrane	79 – 99	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	100 – 583	484	Extracellular Potential
Amino acid modifications
	Glycosylation	310	1	N-linked (GlcNAc...) Potential
	Glycosylation	379	1	N-linked (GlcNAc...) Potential
	Glycosylation	491	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	48	1	P → S in AAA69524. Ref.1
	Sequence conflict	48	1	P → S in AAB01809. Ref.2
	Sequence conflict	126	1	S → T in AAA69524. Ref.1
	Sequence conflict	193	1	L → M in AAA69524. Ref.1
	Sequence conflict	225	1	F → L in AAA69524. Ref.1
	Sequence conflict	225	1	F → L in AAB01809. Ref.2
	Sequence conflict	288	1	R → P in AAA69524. Ref.1
	Sequence conflict	330	1	A → R in AAA69524. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P54360 [UniParc]. Last modified May 24, 2005. Version 2. Checksum: 8AADC85FC85D09A4	FASTA	583	65,490
	10 20 30 40 50 60 MYDIKRLEAG QQKLQQAQQP LGLDLSGQQQ QLTCSVITAP EHRANPNPSS ISQSNPSEAT 70 80 90 100 110 120 HMTLLTLRRR RSLQRRACLL SILAAFVFGM ALGVVVPMFG LPRHQDSPPD LPEEQIQMVA 130 140 150 160 170 180 VEPLSSYRVE FIKETDELSA EQVFRNAFHL EQDKDAPDSM VVKKLDTNDG SIKEFHVQRT 190 200 210 220 230 240 ASGRYRKGPE RRLSKKMPER VQPQETSRSP TTSPTNPTSE HQAGFIEEDV YWGPTVEQAL 250 260 270 280 290 300 PKGFAAKDQV SWERFVGEQG RVVRLEQGCG RMQNRMVVFA DGTRACARYR QNTDQIQGEI 310 320 330 340 350 360 FSYYLGQLLN ISNLAPSAAT VVDTSTPNWA AALGDITQAQ WKERRPVVLT RWLSDLEPAG 370 380 390 400 410 420 IPQPFQPLER HLNKHDVWNL TRHMQSERQA QSQPHGLLKR LGAASSPGSA HQSNAIEETG 430 440 450 460 470 480 TGTETANGAL VQRLIELAQW SDLIVFDYLI ANLDRVVNNL YNFQWNADIM AAPAHNLARQ 490 500 510 520 530 540 SASQLLVFLD NESGLLHGYR LLKKYEAYHS LLLDNLCVFR RPTIDALRRL RAAGAGRRLR 550 560 570 580 DLFERTTSAG VRDVLPSLPD KSVKILVERI DRVLGQVQKC QGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"four-jointed is required for intermediate growth in the proximal-distal axis in Drosophila." Villano J.L., Katz F.N. Development 121:2767-2777(1995) [PubMed: 7555705] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE. Tissue: Eye imaginal disk.
[2]	"Positional information along the dorsal-ventral axis of the Drosophila eye: graded expression of the four-jointed gene." Brodsky M.H., Steller H. Dev. Biol. 173:428-446(1996) [PubMed: 8606003] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Strain: Canton-S.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[6]	"The four-jointed gene is required in the Drosophila eye for ommatidial polarity specification." Zeidler M.P., Perrimon N., Strutt D.I. Curr. Biol. 9:1363-1372(1999) [PubMed: 10607560] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[7]	"Four-jointed interacts with dachs, abelson and enabled and feeds back onto the Notch pathway to affect growth and segmentation in the Drosophila leg." Buckles G.R., Rauskolb C., Villano J.L., Katz F.N. Development 128:3533-3542(2001) [PubMed: 11566858] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[8]	"Cleavage and secretion is not required for four-jointed function in Drosophila patterning." Strutt H., Mundy J., Hofstra K., Strutt D. Development 131:881-890(2004) [PubMed: 14757640] [Abstract] Cited for: FUNCTION, PROTEOLYTIC CLEAVAGE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U28837 mRNA. Translation: AAA69524.1. U44904 mRNA. Translation: AAB01809.1. AE013599 Genomic DNA. Translation: AAF57724.2. AY071147 mRNA. Translation: AAL48769.1.
RefSeq	NP_477483.1. NM_058135.3.
UniGene	Dm.4763.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-917812.
STRING	P54360.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0086754; FBpp0085933; FBgn0000658.
GeneID	37089.
KEGG	dme:Dmel_CG10917.
NMPDR	fig|7227.3.peg.6125.
UCSC	CG10917-RA. d. melanogaster.
Organism-specific databases
CTD	37089.
FlyBase	FBgn0000658. fj.
Phylogenomic databases
eggNOG	inNOG05327.
GeneTree	EMGT00050000016686.
InParanoid	P54360.
OMA	TRACARY.
OrthoDB	EOG4S4MX8.
PhylomeDB	P54360.
Gene expression databases
ArrayExpress	P54360.
Bgee	P54360.
GermOnline	CG10917. Drosophila melanogaster.
Family and domain databases
InterPro	IPR024868. Four-jointed. [Graphical view]
PANTHER	PTHR13147. PTHR13147. 1 hit.
ProtoNet	Search...
Other
NextBio	801889.

Entry information

Entry name

FOJO_DROME

Accession

Primary (citable) accession number: P54360
Secondary accession number(s): Q24176, Q8SZ37, Q9V8E3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 24, 2005
Last modified:	January 25, 2012
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project