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Protein

Vesicle-fusing ATPase 2

Gene

Nsf2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin (By similarity).By similarity

Caution

It is uncertain whether Met-1 or Met-9 is the initiator.Curated

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi510 – 515ATPBy similarity6
Nucleotide bindingi550 – 557ATPBy similarity8

GO - Molecular functioni

  • ATPase activity Source: FlyBase
  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • liquid clearance, open tracheal system Source: FlyBase
  • neuromuscular synaptic transmission Source: FlyBase
  • neurotransmitter secretion Source: FlyBase
  • open tracheal system development Source: FlyBase
  • protein transport Source: UniProtKB-KW
  • regulation of synaptic growth at neuromuscular junction Source: FlyBase
  • SNARE complex disassembly Source: FlyBase
  • synaptic vesicle priming Source: FlyBase
  • terminal branching, open tracheal system Source: FlyBase
  • trachea morphogenesis Source: FlyBase
  • vesicle-mediated transport Source: FlyBase

Keywordsi

Molecular functionHydrolase
Biological processER-Golgi transport, Protein transport, Transport
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.4.6 1994
ReactomeiR-DME-204005 COPII (Coat Protein 2) Mediated Vesicle Transport
R-DME-416993 Trafficking of GluR2-containing AMPA receptors
R-DME-6807878 COPI-mediated anterograde transport
R-DME-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-DME-6811438 Intra-Golgi traffic
R-DME-6811440 Retrograde transport at the Trans-Golgi-Network

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-fusing ATPase 2 (EC:3.6.4.6)
Alternative name(s):
N-ethylmaleimide-sensitive fusion protein 2
Short name:
NEM-sensitive fusion protein 2
Vesicular-fusion protein NSF2
dNsf-2
Short name:
NSF-2
Gene namesi
Name:Nsf2
ORF Names:CG33101
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0266464 Nsf2

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000845671 – 752Vesicle-fusing ATPase 2Add BLAST752

Proteomic databases

PaxDbiP54351
PRIDEiP54351

Expressioni

Tissue specificityi

Nervous system and secretory tissues.1 Publication

Developmental stagei

The highest levels are detected in embryos before and during cellularization. After onset of gastrulation the highest levels of expression appear in embryonic regions that give rise to endodermal and ectodermal tissues including the midgut and hindgut.1 Publication

Gene expression databases

BgeeiFBgn0266464
ExpressionAtlasiP54351 differential
GenevisibleiP54351 DM

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

BioGridi66772, 92 interactors
IntActiP54351, 7 interactors
STRINGi7227.FBpp0082346

Structurei

3D structure databases

ProteinModelPortaliP54351
SMRiP54351
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0741 Eukaryota
COG0464 LUCA
GeneTreeiENSGT00530000064085
InParanoidiP54351
KOiK06027
OMAiIDWVPIG
OrthoDBiEOG091G02LU
PhylomeDBiP54351

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR009010 Asp_de-COase-like_dom_sf
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR004201 Cdc48_dom2
IPR029067 CDC48_domain_2-like_sf
IPR003338 CDC4_N-term_subdom
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00004 AAA, 2 hits
PF02933 CDC48_2, 1 hit
PF02359 CDC48_N, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SM01072 CDC48_2, 1 hit
SM01073 CDC48_N, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
SSF52540 SSF52540, 2 hits
SSF54585 SSF54585, 1 hit
PROSITEiView protein in PROSITE
PS00674 AAA, 1 hit

Sequencei

Sequence statusi: Complete.

P54351-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIEKAHRMR AIKCPTDELS LTNKAIVNVS DFTEEVKYVD ISPGPGLHYI
60 70 80 90 100
FALEKISGPE LPLGHVGFSL VQRKWATLSI NQEIDVRPYR FDASADIITL
110 120 130 140 150
VSFETDFLQK KTTTQEPYDS DEMAKEFLMQ FAGMPLTVGQ TLVFQFKDKK
160 170 180 190 200
FLGLAVKTLE AVDPRTVGDS LPKTRNVRFG RILGNTVVQF EKAENSVLNL
210 220 230 240 250
QGRSKGKIVR QSIINPDWDF GKMGIGGLDK EFNAIFRRAF ASRVFPPELV
260 270 280 290 300
EQLGIKHVKG ILLYGPPGTG KTLMARQIGT MLNAREPKIV NGPQILDKYV
310 320 330 340 350
GESEANIRRL FAEAEEEEKR LGPNSGLHII IFDEIDAICK ARGSVAGNSG
360 370 380 390 400
VHDTVVNQLL AKIDGVEQLN NILVIGMTNR RDMIDEALLR PGRLEVQMEI
410 420 430 440 450
SLPNEQGRVQ ILNIHTKRMR DFNKIASDVD NNEIAAKTKN FSGAELEGLV
460 470 480 490 500
RAAQSTAMNR LIKADSKVHV DPEAMEKLRV TRADFLHALD NDIKPAFGAA
510 520 530 540 550
QEMLENLLAR GIINWGPPVT ELLEDGMLSV QQAKATESSG LVSVLIEGAP
560 570 580 590 600
NSGKSALAAN LAQLSDFPFV KVCSPEDMVG FTESAKCLHI RKIFDDAYRS
610 620 630 640 650
TLSCIVVDNV ERLLDYGPIG PRYSNLTLQA LLVLLKKQPP KGRKLLILCT
660 670 680 690 700
SSRRDVLEEM EMLSAFTSVL HVSNLSTPEN VLAVLDDSDL FSPEELQSIA
710 720 730 740 750
RKMAGKRLCI GIKKLLALID MIRQSEPHQR VIKFLSKMEE EGGLEMDRVQ

GH
Length:752
Mass (Da):83,434
Last modified:October 14, 2008 - v2
Checksum:i0AEF9E88FC3D18AF
GO

Sequence cautioni

The sequence AAC46844 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti680N → H in AAA75044 (PubMed:7642522).Curated1
Sequence conflicti680N → H in AAC46844 (PubMed:7624376).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30502 mRNA Translation: AAA75044.1
AE014297 Genomic DNA Translation: AAF54995.2
BT023784 mRNA Translation: AAZ41793.1
U28836 mRNA Translation: AAC46844.1 Different initiation.
RefSeqiNP_001287318.1, NM_001300389.1
NP_001287319.1, NM_001300390.1
NP_788676.1, NM_176499.2
UniGeneiDm.6688

Genome annotation databases

EnsemblMetazoaiFBtr0082883; FBpp0082346; FBgn0266464
FBtr0344766; FBpp0311094; FBgn0266464
FBtr0344767; FBpp0311095; FBgn0266464
GeneIDi41694
KEGGidme:Dmel_CG33101

Similar proteinsi

Entry informationi

Entry nameiNSF2_DROME
AccessioniPrimary (citable) accession number: P54351
Secondary accession number(s): Q494J2, Q9VFQ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 14, 2008
Last modified: March 28, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health