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Protein

Vesicle-fusing ATPase 2

Gene

Nsf2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi510 – 515ATPBy similarity6
Nucleotide bindingi550 – 557ATPBy similarity8

GO - Molecular functioni

  • ATPase activity Source: FlyBase
  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • Golgi to plasma membrane protein transport Source: GO_Central
  • Golgi vesicle docking Source: GO_Central
  • intra-Golgi vesicle-mediated transport Source: GO_Central
  • neuromuscular synaptic transmission Source: FlyBase
  • neurotransmitter secretion Source: FlyBase
  • regulation of synaptic growth at neuromuscular junction Source: FlyBase
  • SNARE complex disassembly Source: FlyBase
  • synaptic vesicle priming Source: FlyBase
  • terminal branching, open tracheal system Source: FlyBase
  • trachea morphogenesis Source: FlyBase
  • vesicle-mediated transport Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.4.6. 1994.
ReactomeiR-DME-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-DME-416993. Trafficking of GluR2-containing AMPA receptors.
R-DME-6807878. COPI-mediated anterograde transport.
R-DME-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-DME-6811438. Intra-Golgi traffic.
R-DME-6811440. Retrograde transport at the Trans-Golgi-Network.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-fusing ATPase 2 (EC:3.6.4.6)
Alternative name(s):
N-ethylmaleimide-sensitive fusion protein 2
Short name:
NEM-sensitive fusion protein 2
Vesicular-fusion protein NSF2
dNsf-2
Short name:
NSF-2
Gene namesi
Name:Nsf2
ORF Names:CG33101
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0266464. Nsf2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000845671 – 752Vesicle-fusing ATPase 2Add BLAST752

Proteomic databases

PaxDbiP54351.
PRIDEiP54351.

Expressioni

Tissue specificityi

Nervous system and secretory tissues.1 Publication

Developmental stagei

The highest levels are detected in embryos before and during cellularization. After onset of gastrulation the highest levels of expression appear in embryonic regions that give rise to endodermal and ectodermal tissues including the midgut and hindgut.1 Publication

Gene expression databases

BgeeiFBgn0013998.
ExpressionAtlasiP54351. baseline.
GenevisibleiP54351. DM.

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

BioGridi66772. 29 interactors.
IntActiP54351. 3 interactors.
MINTiMINT-1669916.
STRINGi7227.FBpp0082346.

Structurei

3D structure databases

ProteinModelPortaliP54351.
SMRiP54351.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0741. Eukaryota.
COG0464. LUCA.
GeneTreeiENSGT00530000064085.
InParanoidiP54351.
KOiK06027.
OMAiMAHRMRA.
OrthoDBiEOG091G02LU.
PhylomeDBiP54351.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54351-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIEKAHRMR AIKCPTDELS LTNKAIVNVS DFTEEVKYVD ISPGPGLHYI
60 70 80 90 100
FALEKISGPE LPLGHVGFSL VQRKWATLSI NQEIDVRPYR FDASADIITL
110 120 130 140 150
VSFETDFLQK KTTTQEPYDS DEMAKEFLMQ FAGMPLTVGQ TLVFQFKDKK
160 170 180 190 200
FLGLAVKTLE AVDPRTVGDS LPKTRNVRFG RILGNTVVQF EKAENSVLNL
210 220 230 240 250
QGRSKGKIVR QSIINPDWDF GKMGIGGLDK EFNAIFRRAF ASRVFPPELV
260 270 280 290 300
EQLGIKHVKG ILLYGPPGTG KTLMARQIGT MLNAREPKIV NGPQILDKYV
310 320 330 340 350
GESEANIRRL FAEAEEEEKR LGPNSGLHII IFDEIDAICK ARGSVAGNSG
360 370 380 390 400
VHDTVVNQLL AKIDGVEQLN NILVIGMTNR RDMIDEALLR PGRLEVQMEI
410 420 430 440 450
SLPNEQGRVQ ILNIHTKRMR DFNKIASDVD NNEIAAKTKN FSGAELEGLV
460 470 480 490 500
RAAQSTAMNR LIKADSKVHV DPEAMEKLRV TRADFLHALD NDIKPAFGAA
510 520 530 540 550
QEMLENLLAR GIINWGPPVT ELLEDGMLSV QQAKATESSG LVSVLIEGAP
560 570 580 590 600
NSGKSALAAN LAQLSDFPFV KVCSPEDMVG FTESAKCLHI RKIFDDAYRS
610 620 630 640 650
TLSCIVVDNV ERLLDYGPIG PRYSNLTLQA LLVLLKKQPP KGRKLLILCT
660 670 680 690 700
SSRRDVLEEM EMLSAFTSVL HVSNLSTPEN VLAVLDDSDL FSPEELQSIA
710 720 730 740 750
RKMAGKRLCI GIKKLLALID MIRQSEPHQR VIKFLSKMEE EGGLEMDRVQ

GH
Length:752
Mass (Da):83,434
Last modified:October 14, 2008 - v2
Checksum:i0AEF9E88FC3D18AF
GO

Sequence cautioni

The sequence AAC46844 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti680N → H in AAA75044 (PubMed:7642522).Curated1
Sequence conflicti680N → H in AAC46844 (PubMed:7624376).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30502 mRNA. Translation: AAA75044.1.
AE014297 Genomic DNA. Translation: AAF54995.2.
BT023784 mRNA. Translation: AAZ41793.1.
U28836 mRNA. Translation: AAC46844.1. Different initiation.
RefSeqiNP_001287318.1. NM_001300389.1.
NP_001287319.1. NM_001300390.1.
NP_788676.1. NM_176499.2.
UniGeneiDm.6688.

Genome annotation databases

EnsemblMetazoaiFBtr0082883; FBpp0082346; FBgn0266464.
FBtr0344766; FBpp0311094; FBgn0266464.
FBtr0344767; FBpp0311095; FBgn0266464.
GeneIDi41694.
KEGGidme:Dmel_CG33101.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30502 mRNA. Translation: AAA75044.1.
AE014297 Genomic DNA. Translation: AAF54995.2.
BT023784 mRNA. Translation: AAZ41793.1.
U28836 mRNA. Translation: AAC46844.1. Different initiation.
RefSeqiNP_001287318.1. NM_001300389.1.
NP_001287319.1. NM_001300390.1.
NP_788676.1. NM_176499.2.
UniGeneiDm.6688.

3D structure databases

ProteinModelPortaliP54351.
SMRiP54351.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi66772. 29 interactors.
IntActiP54351. 3 interactors.
MINTiMINT-1669916.
STRINGi7227.FBpp0082346.

Proteomic databases

PaxDbiP54351.
PRIDEiP54351.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0082883; FBpp0082346; FBgn0266464.
FBtr0344766; FBpp0311094; FBgn0266464.
FBtr0344767; FBpp0311095; FBgn0266464.
GeneIDi41694.
KEGGidme:Dmel_CG33101.

Organism-specific databases

CTDi41694.
FlyBaseiFBgn0266464. Nsf2.

Phylogenomic databases

eggNOGiKOG0741. Eukaryota.
COG0464. LUCA.
GeneTreeiENSGT00530000064085.
InParanoidiP54351.
KOiK06027.
OMAiMAHRMRA.
OrthoDBiEOG091G02LU.
PhylomeDBiP54351.

Enzyme and pathway databases

BRENDAi3.6.4.6. 1994.
ReactomeiR-DME-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-DME-416993. Trafficking of GluR2-containing AMPA receptors.
R-DME-6807878. COPI-mediated anterograde transport.
R-DME-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-DME-6811438. Intra-Golgi traffic.
R-DME-6811440. Retrograde transport at the Trans-Golgi-Network.

Miscellaneous databases

GenomeRNAii41694.
PROiP54351.

Gene expression databases

BgeeiFBgn0013998.
ExpressionAtlasiP54351. baseline.
GenevisibleiP54351. DM.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNSF2_DROME
AccessioniPrimary (citable) accession number: P54351
Secondary accession number(s): Q494J2, Q9VFQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 14, 2008
Last modified: November 30, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-9 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.