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P54351

- NSF2_DROME

UniProt

P54351 - NSF2_DROME

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Protein

Vesicle-fusing ATPase 2

Gene

Nsf2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg(2+) ion per subunit.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi510 – 5156ATPBy similarity
Nucleotide bindingi550 – 5578ATPBy similarity

GO - Molecular functioni

  1. ATPase activity Source: FlyBase
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. neuromuscular synaptic transmission Source: FlyBase
  3. neurotransmitter secretion Source: FlyBase
  4. protein transport Source: UniProtKB-KW
  5. regulation of synaptic growth at neuromuscular junction Source: FlyBase
  6. synaptic vesicle priming Source: FlyBase
  7. terminal branching, open tracheal system Source: FlyBase
  8. trachea morphogenesis Source: FlyBase
  9. vesicle-mediated transport Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_263519. Trafficking of GluR2-containing AMPA receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-fusing ATPase 2 (EC:3.6.4.6)
Alternative name(s):
N-ethylmaleimide-sensitive fusion protein 2
Short name:
NEM-sensitive fusion protein 2
Vesicular-fusion protein NSF2
dNsf-2
Short name:
NSF-2
Gene namesi
Name:Nsf2
ORF Names:CG33101
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0266464. Nsf2.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 752752Vesicle-fusing ATPase 2PRO_0000084567Add
BLAST

Proteomic databases

PaxDbiP54351.
PRIDEiP54351.

Expressioni

Tissue specificityi

Nervous system and secretory tissues.1 Publication

Developmental stagei

The highest levels are detected in embryos before and during cellularization. After onset of gastrulation the highest levels of expression appear in embryonic regions that give rise to endodermal and ectodermal tissues including the midgut and hindgut.1 Publication

Gene expression databases

BgeeiP54351.

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

BioGridi66772. 29 interactions.
MINTiMINT-1669916.

Structurei

3D structure databases

ProteinModelPortaliP54351.
SMRiP54351. Positions 9-174, 193-747.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0464.
GeneTreeiENSGT00530000064085.
InParanoidiP54351.
KOiK06027.
OMAiFKDKERS.
OrthoDBiEOG7P8P7F.
PhylomeDBiP54351.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54351-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSIEKAHRMR AIKCPTDELS LTNKAIVNVS DFTEEVKYVD ISPGPGLHYI
60 70 80 90 100
FALEKISGPE LPLGHVGFSL VQRKWATLSI NQEIDVRPYR FDASADIITL
110 120 130 140 150
VSFETDFLQK KTTTQEPYDS DEMAKEFLMQ FAGMPLTVGQ TLVFQFKDKK
160 170 180 190 200
FLGLAVKTLE AVDPRTVGDS LPKTRNVRFG RILGNTVVQF EKAENSVLNL
210 220 230 240 250
QGRSKGKIVR QSIINPDWDF GKMGIGGLDK EFNAIFRRAF ASRVFPPELV
260 270 280 290 300
EQLGIKHVKG ILLYGPPGTG KTLMARQIGT MLNAREPKIV NGPQILDKYV
310 320 330 340 350
GESEANIRRL FAEAEEEEKR LGPNSGLHII IFDEIDAICK ARGSVAGNSG
360 370 380 390 400
VHDTVVNQLL AKIDGVEQLN NILVIGMTNR RDMIDEALLR PGRLEVQMEI
410 420 430 440 450
SLPNEQGRVQ ILNIHTKRMR DFNKIASDVD NNEIAAKTKN FSGAELEGLV
460 470 480 490 500
RAAQSTAMNR LIKADSKVHV DPEAMEKLRV TRADFLHALD NDIKPAFGAA
510 520 530 540 550
QEMLENLLAR GIINWGPPVT ELLEDGMLSV QQAKATESSG LVSVLIEGAP
560 570 580 590 600
NSGKSALAAN LAQLSDFPFV KVCSPEDMVG FTESAKCLHI RKIFDDAYRS
610 620 630 640 650
TLSCIVVDNV ERLLDYGPIG PRYSNLTLQA LLVLLKKQPP KGRKLLILCT
660 670 680 690 700
SSRRDVLEEM EMLSAFTSVL HVSNLSTPEN VLAVLDDSDL FSPEELQSIA
710 720 730 740 750
RKMAGKRLCI GIKKLLALID MIRQSEPHQR VIKFLSKMEE EGGLEMDRVQ

GH
Length:752
Mass (Da):83,434
Last modified:October 14, 2008 - v2
Checksum:i0AEF9E88FC3D18AF
GO

Sequence cautioni

The sequence AAC46844.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti680 – 6801N → H in AAA75044. (PubMed:7642522)Curated
Sequence conflicti680 – 6801N → H in AAC46844. (PubMed:7624376)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30502 mRNA. Translation: AAA75044.1.
AE014297 Genomic DNA. Translation: AAF54995.2.
BT023784 mRNA. Translation: AAZ41793.1.
U28836 mRNA. Translation: AAC46844.1. Different initiation.
RefSeqiNP_001287318.1. NM_001300389.1.
NP_001287319.1. NM_001300390.1.
NP_788676.1. NM_176499.2.
UniGeneiDm.6688.

Genome annotation databases

EnsemblMetazoaiFBtr0082883; FBpp0082346; FBgn0013998.
GeneIDi41694.
KEGGidme:Dmel_CG33101.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U30502 mRNA. Translation: AAA75044.1 .
AE014297 Genomic DNA. Translation: AAF54995.2 .
BT023784 mRNA. Translation: AAZ41793.1 .
U28836 mRNA. Translation: AAC46844.1 . Different initiation.
RefSeqi NP_001287318.1. NM_001300389.1.
NP_001287319.1. NM_001300390.1.
NP_788676.1. NM_176499.2.
UniGenei Dm.6688.

3D structure databases

ProteinModelPortali P54351.
SMRi P54351. Positions 9-174, 193-747.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 66772. 29 interactions.
MINTi MINT-1669916.

Proteomic databases

PaxDbi P54351.
PRIDEi P54351.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0082883 ; FBpp0082346 ; FBgn0013998 .
GeneIDi 41694.
KEGGi dme:Dmel_CG33101.

Organism-specific databases

CTDi 41694.
FlyBasei FBgn0266464. Nsf2.

Phylogenomic databases

eggNOGi COG0464.
GeneTreei ENSGT00530000064085.
InParanoidi P54351.
KOi K06027.
OMAi FKDKERS.
OrthoDBi EOG7P8P7F.
PhylomeDBi P54351.

Enzyme and pathway databases

Reactomei REACT_263519. Trafficking of GluR2-containing AMPA receptors.

Miscellaneous databases

GenomeRNAii 41694.
NextBioi 825088.

Gene expression databases

Bgeei P54351.

Family and domain databases

Gene3Di 3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEi PS00674. AAA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Distinct roles for N-ethylmaleimide-sensitive fusion protein (NSF) suggested by the identification of a second Drosophila NSF homolog."
    Pallanck L., Ordway R.W., Ramaswami M., Chi W.Y., Krishnan K.S., Ganetzky B.
    J. Biol. Chem. 270:18742-18744(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Canton-S.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  5. "Identification of a second homolog of N-ethylmaleimide-sensitive fusion protein that is expressed in the nervous system and secretory tissues of Drosophila."
    Boulianne G.L., Trimble W.S.
    Proc. Natl. Acad. Sci. U.S.A. 92:7095-7099(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-752.
    Tissue: Head.

Entry informationi

Entry nameiNSF2_DROME
AccessioniPrimary (citable) accession number: P54351
Secondary accession number(s): Q494J2, Q9VFQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 14, 2008
Last modified: November 26, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-9 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3