Reviewed, UniProtKB/Swiss-Prot P54351 (NSF2_DROME)

Names and origin

Protein names

Recommended name:
    Vesicle-fusing ATPase 2
    EC=3.6.4.6
Alternative name(s):
    N-ethylmaleimide-sensitive fusion protein 2
      Short name=NEM-sensitive fusion protein 2
    Vesicular-fusion protein NSF2
    dNsf-2
      Short name=NSF-2

Gene names

Name:	Nsf2
ORF Names:	CG33101

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	752 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Homohexamer By similarity.
Subcellular location	Cytoplasm. Ref.1
Tissue specificity	Nervous system and secretory tissues. Ref.1
Developmental stage	The highest levels are detected in embryos before and during cellularization. After onset of gastrulation the highest levels of expression appear in embryonic regions that give rise to endodermal and ectodermal tissues including the midgut and hindgut. Ref.1
Sequence similarities	Belongs to the AAA ATPase family.
Caution	It is uncertain whether Met-1 or Met-9 is the initiator.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	neuromuscular synaptic transmission Inferred from mutant phenotype. Source: FlyBase protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of synaptic growth at neuromuscular junction Inferred from mutant phenotype. Source: FlyBase synaptic vesicle priming Non-traceable author statement. Source: FlyBase
Cellular component	cytoplasm Non-traceable author statement. Source: FlyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 752	752	Vesicle-fusing ATPase 2		PRO_0000084567
Regions
	Nucleotide binding	265 – 272	8	ATP Potential
	Nucleotide binding	548 – 555	8	ATP Potential
Experimental info
	Sequence conflict	680	1	N → H in AAA75044. Ref.1
	Sequence conflict	680	1	N → H in AAC46844. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P54351-1 [UniParc]. Last modified October 14, 2008. Version 2. Checksum: 0AEF9E88FC3D18AF	FASTA	752	83,434
	10 20 30 40 50 60 MSIEKAHRMR AIKCPTDELS LTNKAIVNVS DFTEEVKYVD ISPGPGLHYI FALEKISGPE 70 80 90 100 110 120 LPLGHVGFSL VQRKWATLSI NQEIDVRPYR FDASADIITL VSFETDFLQK KTTTQEPYDS 130 140 150 160 170 180 DEMAKEFLMQ FAGMPLTVGQ TLVFQFKDKK FLGLAVKTLE AVDPRTVGDS LPKTRNVRFG 190 200 210 220 230 240 RILGNTVVQF EKAENSVLNL QGRSKGKIVR QSIINPDWDF GKMGIGGLDK EFNAIFRRAF 250 260 270 280 290 300 ASRVFPPELV EQLGIKHVKG ILLYGPPGTG KTLMARQIGT MLNAREPKIV NGPQILDKYV 310 320 330 340 350 360 GESEANIRRL FAEAEEEEKR LGPNSGLHII IFDEIDAICK ARGSVAGNSG VHDTVVNQLL 370 380 390 400 410 420 AKIDGVEQLN NILVIGMTNR RDMIDEALLR PGRLEVQMEI SLPNEQGRVQ ILNIHTKRMR 430 440 450 460 470 480 DFNKIASDVD NNEIAAKTKN FSGAELEGLV RAAQSTAMNR LIKADSKVHV DPEAMEKLRV 490 500 510 520 530 540 TRADFLHALD NDIKPAFGAA QEMLENLLAR GIINWGPPVT ELLEDGMLSV QQAKATESSG 550 560 570 580 590 600 LVSVLIEGAP NSGKSALAAN LAQLSDFPFV KVCSPEDMVG FTESAKCLHI RKIFDDAYRS 610 620 630 640 650 660 TLSCIVVDNV ERLLDYGPIG PRYSNLTLQA LLVLLKKQPP KGRKLLILCT SSRRDVLEEM 670 680 690 700 710 720 EMLSAFTSVL HVSNLSTPEN VLAVLDDSDL FSPEELQSIA RKMAGKRLCI GIKKLLALID 730 740 750 MIRQSEPHQR VIKFLSKMEE EGGLEMDRVQ GH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Distinct roles for N-ethylmaleimide-sensitive fusion protein (NSF) suggested by the identification of a second Drosophila NSF homolog." Pallanck L., Ordway R.W., Ramaswami M., Chi W.Y., Krishnan K.S., Ganetzky B. J. Biol. Chem. 270:18742-18744(1995) [PubMed: 7642522] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: Canton-S.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Identification of a second homolog of N-ethylmaleimide-sensitive fusion protein that is expressed in the nervous system and secretory tissues of Drosophila." Boulianne G.L., Trimble W.S. Proc. Natl. Acad. Sci. U.S.A. 92:7095-7099(1995) [PubMed: 7624376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-752. Tissue: Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U30502 mRNA. Translation: AAA75044.1. AE014297 Genomic DNA. Translation: AAF54995.2. BT023784 mRNA. Translation: AAZ41793.1. U28836 mRNA. Translation: AAC46844.1. Different initiation.
RefSeq	NP_788676.1.
UniGene	Dm.6688
3D structure databases
HSSP	HSSP built from PDB template 1NSF based on UniProtKB P18708.
ModBase	Search...
Protein-protein interaction databases
IntAct	P54351. 3 interactions.
Proteomic databases
PRIDE	P54351.
Genome annotation databases
Ensembl	FBgn0013998. Drosophila melanogaster. [Contig view]
GeneID	41694.
KEGG	dme:Dmel_CG33101.
Organism-specific databases
FlyBase	FBgn0013998. Nsf2.
Phylogenomic databases
HOGENOM	P54351.
OMA	P54351. VRTSPNH.
Enzyme and pathway databases
BRENDA	3.6.4.6. 48.
Gene expression databases
ArrayExpress	P54351.
GermOnline	CG33101. Drosophila melanogaster.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR003338. ATPase_AAA_VAT_N. IPR004201. Cell_division_protein_CDC48_2. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF02933. CDC48_2. 1 hit. PF02359. CDC48_N. 1 hit. [Graphical view]
SMART	SM00382. AAA. 2 hits. [Graphical view]
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	825088.

Entry information

Entry name

NSF2_DROME

Accession

Primary (citable) accession number: P54351
Secondary accession number(s): Q494J2, Q9VFQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 14, 2008
Last modified:	June 16, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project