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P54350

- WEE1_DROME

UniProt

P54350 - WEE1_DROME

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Protein

Wee1-like protein kinase

Gene

wee

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Could act as a negative regulator of entry into mitosis (G2 to M transition). This kinase specifically phosphorylates and inactivates cyclin B1-complexed CDC2.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Enzyme regulationi

Negatively regulated by phosphorylation in the M-phase.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei268 – 2681ATPPROSITE-ProRule annotation
Active sitei361 – 3611Proton acceptorPROSITE-ProRule annotation
Metal bindingi366 – 3661Magnesium; via carbonyl oxygenBy similarity
Metal bindingi412 – 4121Magnesium; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi245 – 2539ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  4. protein kinase activity Source: FlyBase
  5. protein tyrosine kinase activity Source: FlyBase

GO - Biological processi

  1. centrosome localization Source: FlyBase
  2. centrosome separation Source: FlyBase
  3. embryonic development via the syncytial blastoderm Source: FlyBase
  4. mitotic cell cycle Source: FlyBase
  5. mitotic cell cycle checkpoint Source: FlyBase
  6. mitotic nuclear division Source: UniProtKB-KW
  7. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: FlyBase
  8. peptidyl-tyrosine phosphorylation Source: GOC
  9. protein phosphorylation Source: FlyBase
  10. spindle assembly Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 1994.
ReactomeiREACT_180258. Factors involved in megakaryocyte development and platelet production.
REACT_208929. Polo-like kinase mediated events.
REACT_225636. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
REACT_237453. G2/M DNA replication checkpoint.
REACT_246054. Cyclin A:Cdk2-associated events at S phase entry.
REACT_251099. Cyclin A/B1 associated events during G2/M transition.
SignaLinkiP54350.

Names & Taxonomyi

Protein namesi
Recommended name:
Wee1-like protein kinase (EC:2.7.10.2)
Short name:
Dwee1
Gene namesi
Name:wee
ORF Names:CG4488
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0011737. wee.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. microtubule associated complex Source: FlyBase
  2. nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 609609Wee1-like protein kinasePRO_0000086814Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei23 – 231Phosphoserine1 Publication
Modified residuei25 – 251Phosphoserine1 Publication
Modified residuei27 – 271Phosphoserine1 Publication
Modified residuei47 – 471Phosphothreonine1 Publication
Modified residuei52 – 521Phosphoserine1 Publication
Modified residuei165 – 1651Phosphothreonine1 Publication
Modified residuei168 – 1681Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated during M and G1 phases.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP54350.
PRIDEiP54350.

Expressioni

Tissue specificityi

Expressed in embryos; expression remains high in the proliferating cells of the central nervous system well after cells in the rest of the embryo have ceased dividing.1 Publication

Developmental stagei

Expressed both maternally and zygotically during postblastoderm divisions of embryogenesis.1 Publication

Gene expression databases

BgeeiP54350.
ExpressionAtlasiP54350. differential.

Interactioni

Protein-protein interaction databases

BioGridi60116. 15 interactions.
DIPiDIP-23536N.
IntActiP54350. 2 interactions.
MINTiMINT-885153.
STRINGi7227.FBpp0078999.

Structurei

3D structure databases

ProteinModelPortaliP54350.
SMRiP54350. Positions 214-587.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini239 – 517279Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi122 – 1265Poly-Ala

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00530000063230.
InParanoidiP54350.
KOiK06632.
OMAiCIYAIKK.
OrthoDBiEOG7N63M9.
PhylomeDBiP54350.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54350-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAFRQSEHEM SVTSLDSSVE LRSRSPSPQV FNPRKLRFAD DDFDKDTPEG
60 70 80 90 100
ASPQHPLQQR PKLSSGEEQQ LDSKIGKEGG DGDVSMSPPC QKVRALRLFS
110 120 130 140 150
TPATPKTILQ KSTTQCSNHL SAAAAAVNAS RRSDDLFRLS ERPRSLPLHN
160 170 180 190 200
RKLPTQDTAN VNPFTPDSLM AHNKKRCRTQ FGRENLNLNV NAMQKYLLSD
210 220 230 240 250
ACDDDVTEEA GDSMREIHQQ APKRLALHDT NISRFKREFM QVNVIGVGEF
260 270 280 290 300
GVVFQCVNRL DGCIYAIKKS KKPVAGSSFE KRALNEVWAH AVLGKHDNVV
310 320 330 340 350
RYYSAWAEDD HMLIQNEFCD GGSLHARIQD HCLGEAELKI VLMHVIEGLR
360 370 380 390 400
YIHSNDLVHM DLKPENIFST MNPNAHKLVE VQPQQTKDDD GMDSVYEELR
410 420 430 440 450
HSENLVTYKI GDLGHVTSVK EPYVEEGDCR YLPKEILHED YSNLFKADIF
460 470 480 490 500
SLGITLFEAA GGGPLPKNGP EWHNLRDGKV PILPSLSRDF NELIAQMMHP
510 520 530 540 550
YPDKRPTSQS IFSHPILSAV DSKSKLQLGL ELTVEKRKNE ILMNKLREAK
560 570 580 590 600
KQIKLLEQRV NLLAVTNNPD SLDGQRCLRS FTRRMRTPFS SHGKFDSISD

RNKNVITNI
Length:609
Mass (Da):68,809
Last modified:December 1, 2000 - v2
Checksum:i7E371EFDA2A5CD96
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 362KL → NV in AAC46913. (PubMed:8573790)Curated
Sequence conflicti72 – 721D → G in AAC46913. (PubMed:8573790)Curated
Sequence conflicti275 – 2751A → R in AAC46913. (PubMed:8573790)Curated
Sequence conflicti567 – 5671N → K in AAC46913. (PubMed:8573790)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17223 mRNA. Translation: AAC46913.2.
AE014134 Genomic DNA. Translation: AAF52453.2.
AY118942 mRNA. Translation: AAM50802.1.
RefSeqiNP_001260167.1. NM_001273238.1.
NP_477035.1. NM_057687.4.
UniGeneiDm.3106.

Genome annotation databases

EnsemblMetazoaiFBtr0079371; FBpp0078999; FBgn0011737.
FBtr0332329; FBpp0304607; FBgn0011737.
GeneIDi33965.
KEGGidme:Dmel_CG4488.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17223 mRNA. Translation: AAC46913.2 .
AE014134 Genomic DNA. Translation: AAF52453.2 .
AY118942 mRNA. Translation: AAM50802.1 .
RefSeqi NP_001260167.1. NM_001273238.1.
NP_477035.1. NM_057687.4.
UniGenei Dm.3106.

3D structure databases

ProteinModelPortali P54350.
SMRi P54350. Positions 214-587.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 60116. 15 interactions.
DIPi DIP-23536N.
IntActi P54350. 2 interactions.
MINTi MINT-885153.
STRINGi 7227.FBpp0078999.

Proteomic databases

PaxDbi P54350.
PRIDEi P54350.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0079371 ; FBpp0078999 ; FBgn0011737 .
FBtr0332329 ; FBpp0304607 ; FBgn0011737 .
GeneIDi 33965.
KEGGi dme:Dmel_CG4488.

Organism-specific databases

CTDi 7465.
FlyBasei FBgn0011737. wee.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00530000063230.
InParanoidi P54350.
KOi K06632.
OMAi CIYAIKK.
OrthoDBi EOG7N63M9.
PhylomeDBi P54350.

Enzyme and pathway databases

BRENDAi 2.7.10.2. 1994.
Reactomei REACT_180258. Factors involved in megakaryocyte development and platelet production.
REACT_208929. Polo-like kinase mediated events.
REACT_225636. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
REACT_237453. G2/M DNA replication checkpoint.
REACT_246054. Cyclin A:Cdk2-associated events at S phase entry.
REACT_251099. Cyclin A/B1 associated events during G2/M transition.
SignaLinki P54350.

Miscellaneous databases

GenomeRNAii 33965.
NextBioi 786174.
PROi P54350.

Gene expression databases

Bgeei P54350.
ExpressionAtlasi P54350. differential.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PIRSFi PIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMi SSF56112. SSF56112. 2 hits.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Drosophila Wee1 kinase rescues fission yeast from mitotic catastrophe and phosphorylates Drosophila Cdc2 in vitro."
    Campbell S.D., Sprenger F., Edgar B.A., O'Farrell P.H.
    Mol. Biol. Cell 6:1333-1347(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. Campbell S.D.
    Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO C-TERMINUS.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  6. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23; SER-25; SER-27; THR-47; SER-52; THR-165 AND SER-168, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiWEE1_DROME
AccessioniPrimary (citable) accession number: P54350
Secondary accession number(s): Q9VM70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 1, 2000
Last modified: November 26, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3