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Protein

Wee1-like protein kinase

Gene

Wee1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Could act as a negative regulator of entry into mitosis (G2 to M transition). This kinase specifically phosphorylates and inactivates cyclin B1-complexed CDC2.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Enzyme regulationi

Negatively regulated by phosphorylation in the M-phase.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei268ATPPROSITE-ProRule annotation1
Active sitei361Proton acceptorPROSITE-ProRule annotation1
Metal bindingi366Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi412Magnesium; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi245 – 253ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • magnesium ion binding Source: InterPro
  • non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  • protein kinase activity Source: FlyBase
  • protein tyrosine kinase activity Source: FlyBase

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • centrosome localization Source: FlyBase
  • centrosome separation Source: FlyBase
  • embryonic development via the syncytial blastoderm Source: FlyBase
  • mitotic cell cycle Source: FlyBase
  • mitotic cell cycle checkpoint Source: FlyBase
  • mitotic nuclear division Source: UniProtKB-KW
  • negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: FlyBase
  • peptidyl-tyrosine phosphorylation Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • spindle assembly Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 1994.
ReactomeiR-DME-156711. Polo-like kinase mediated events.
R-DME-69202. Cyclin E associated events during G1/S transition.
R-DME-69273. Cyclin A/B1 associated events during G2/M transition.
R-DME-69478. G2/M DNA replication checkpoint.
R-DME-69656. Cyclin A:Cdk2-associated events at S phase entry.
R-DME-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
SignaLinkiP54350.

Names & Taxonomyi

Protein namesi
Recommended name:
Wee1-like protein kinase (EC:2.7.10.2)
Short name:
Dwee1
Gene namesi
Name:Wee1Imported
Synonyms:wee
ORF Names:CG4488Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0011737. Wee1.

Subcellular locationi

GO - Cellular componenti

  • microtubule associated complex Source: FlyBase
  • nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000868141 – 609Wee1-like protein kinaseAdd BLAST609

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei23Phosphoserine1 Publication1
Modified residuei25Phosphoserine1 Publication1
Modified residuei27Phosphoserine1 Publication1
Modified residuei47Phosphothreonine1 Publication1
Modified residuei52Phosphoserine1 Publication1
Modified residuei165Phosphothreonine1 Publication1
Modified residuei168Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated during M and G1 phases.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP54350.
PRIDEiP54350.

PTM databases

iPTMnetiP54350.

Expressioni

Tissue specificityi

Expressed in embryos; expression remains high in the proliferating cells of the central nervous system well after cells in the rest of the embryo have ceased dividing.1 Publication

Developmental stagei

Expressed both maternally and zygotically during postblastoderm divisions of embryogenesis.1 Publication

Gene expression databases

BgeeiFBgn0011737.
ExpressionAtlasiP54350. baseline.
GenevisibleiP54350. DM.

Interactioni

Protein-protein interaction databases

BioGridi60116. 15 interactors.
DIPiDIP-23536N.
IntActiP54350. 3 interactors.
MINTiMINT-885153.
STRINGi7227.FBpp0304607.

Structurei

3D structure databases

ProteinModelPortaliP54350.
SMRiP54350.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini239 – 517Protein kinasePROSITE-ProRule annotationAdd BLAST279

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi122 – 126Poly-Ala5

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0601. Eukaryota.
ENOG410XS1M. LUCA.
GeneTreeiENSGT00530000063230.
InParanoidiP54350.
KOiK06632.
OMAiVNAMQKY.
OrthoDBiEOG091G049H.
PhylomeDBiP54350.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54350-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFRQSEHEM SVTSLDSSVE LRSRSPSPQV FNPRKLRFAD DDFDKDTPEG
60 70 80 90 100
ASPQHPLQQR PKLSSGEEQQ LDSKIGKEGG DGDVSMSPPC QKVRALRLFS
110 120 130 140 150
TPATPKTILQ KSTTQCSNHL SAAAAAVNAS RRSDDLFRLS ERPRSLPLHN
160 170 180 190 200
RKLPTQDTAN VNPFTPDSLM AHNKKRCRTQ FGRENLNLNV NAMQKYLLSD
210 220 230 240 250
ACDDDVTEEA GDSMREIHQQ APKRLALHDT NISRFKREFM QVNVIGVGEF
260 270 280 290 300
GVVFQCVNRL DGCIYAIKKS KKPVAGSSFE KRALNEVWAH AVLGKHDNVV
310 320 330 340 350
RYYSAWAEDD HMLIQNEFCD GGSLHARIQD HCLGEAELKI VLMHVIEGLR
360 370 380 390 400
YIHSNDLVHM DLKPENIFST MNPNAHKLVE VQPQQTKDDD GMDSVYEELR
410 420 430 440 450
HSENLVTYKI GDLGHVTSVK EPYVEEGDCR YLPKEILHED YSNLFKADIF
460 470 480 490 500
SLGITLFEAA GGGPLPKNGP EWHNLRDGKV PILPSLSRDF NELIAQMMHP
510 520 530 540 550
YPDKRPTSQS IFSHPILSAV DSKSKLQLGL ELTVEKRKNE ILMNKLREAK
560 570 580 590 600
KQIKLLEQRV NLLAVTNNPD SLDGQRCLRS FTRRMRTPFS SHGKFDSISD

RNKNVITNI
Length:609
Mass (Da):68,809
Last modified:December 1, 2000 - v2
Checksum:i7E371EFDA2A5CD96
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35 – 36KL → NV in AAC46913 (PubMed:8573790).Curated2
Sequence conflicti72D → G in AAC46913 (PubMed:8573790).Curated1
Sequence conflicti275A → R in AAC46913 (PubMed:8573790).Curated1
Sequence conflicti567N → K in AAC46913 (PubMed:8573790).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17223 mRNA. Translation: AAC46913.2.
AE014134 Genomic DNA. Translation: AAF52453.2.
AY118942 mRNA. Translation: AAM50802.1.
RefSeqiNP_001260167.1. NM_001273238.1.
NP_477035.1. NM_057687.4.
UniGeneiDm.3106.

Genome annotation databases

EnsemblMetazoaiFBtr0079371; FBpp0078999; FBgn0011737.
FBtr0332329; FBpp0304607; FBgn0011737.
GeneIDi33965.
KEGGidme:Dmel_CG4488.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17223 mRNA. Translation: AAC46913.2.
AE014134 Genomic DNA. Translation: AAF52453.2.
AY118942 mRNA. Translation: AAM50802.1.
RefSeqiNP_001260167.1. NM_001273238.1.
NP_477035.1. NM_057687.4.
UniGeneiDm.3106.

3D structure databases

ProteinModelPortaliP54350.
SMRiP54350.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi60116. 15 interactors.
DIPiDIP-23536N.
IntActiP54350. 3 interactors.
MINTiMINT-885153.
STRINGi7227.FBpp0304607.

PTM databases

iPTMnetiP54350.

Proteomic databases

PaxDbiP54350.
PRIDEiP54350.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0079371; FBpp0078999; FBgn0011737.
FBtr0332329; FBpp0304607; FBgn0011737.
GeneIDi33965.
KEGGidme:Dmel_CG4488.

Organism-specific databases

CTDi7465.
FlyBaseiFBgn0011737. Wee1.

Phylogenomic databases

eggNOGiKOG0601. Eukaryota.
ENOG410XS1M. LUCA.
GeneTreeiENSGT00530000063230.
InParanoidiP54350.
KOiK06632.
OMAiVNAMQKY.
OrthoDBiEOG091G049H.
PhylomeDBiP54350.

Enzyme and pathway databases

BRENDAi2.7.10.2. 1994.
ReactomeiR-DME-156711. Polo-like kinase mediated events.
R-DME-69202. Cyclin E associated events during G1/S transition.
R-DME-69273. Cyclin A/B1 associated events during G2/M transition.
R-DME-69478. G2/M DNA replication checkpoint.
R-DME-69656. Cyclin A:Cdk2-associated events at S phase entry.
R-DME-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
SignaLinkiP54350.

Miscellaneous databases

GenomeRNAii33965.
PROiP54350.

Gene expression databases

BgeeiFBgn0011737.
ExpressionAtlasiP54350. baseline.
GenevisibleiP54350. DM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWEE1_DROME
AccessioniPrimary (citable) accession number: P54350
Secondary accession number(s): Q9VM70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 1, 2000
Last modified: November 30, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.