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Protein

Wee1-like protein kinase

Gene

Wee1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a negative regulator of entry into mitosis (G2 to M transition) (PubMed:8573790, PubMed:15581871). This kinase specifically phosphorylates and inactivates cyclin B1-complexed CDC2 (PubMed:8573790, PubMed:15581871).2 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Enzyme regulationi

Negatively regulated by phosphorylation in the M-phase.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei268ATPPROSITE-ProRule annotation1
Active sitei361Proton acceptorPROSITE-ProRule annotation1
Metal bindingi366Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi412Magnesium; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi245 – 253ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • magnesium ion binding Source: InterPro
  • non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  • protein kinase activity Source: FlyBase
  • protein tyrosine kinase activity Source: FlyBase

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • centrosome localization Source: FlyBase
  • centrosome separation Source: FlyBase
  • embryonic development via the syncytial blastoderm Source: FlyBase
  • mitotic cell cycle checkpoint Source: FlyBase
  • negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: FlyBase
  • peptidyl-tyrosine phosphorylation Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • spindle assembly Source: FlyBase

Keywordsi

Molecular functionKinase, Transferase, Tyrosine-protein kinase
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2 1994
ReactomeiR-DME-156711 Polo-like kinase mediated events
R-DME-69202 Cyclin E associated events during G1/S transition
R-DME-69273 Cyclin A/B1/B2 associated events during G2/M transition
R-DME-69478 G2/M DNA replication checkpoint
R-DME-69656 Cyclin A:Cdk2-associated events at S phase entry
R-DME-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex
SignaLinkiP54350

Names & Taxonomyi

Protein namesi
Recommended name:
Wee1-like protein kinase (EC:2.7.10.2)
Short name:
Dwee1
Gene namesi
Name:Wee1Imported
Synonyms:wee
ORF Names:CG4488Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0011737 Wee1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000868141 – 609Wee1-like protein kinaseAdd BLAST609

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei23Phosphoserine1 Publication1
Modified residuei25Phosphoserine1 Publication1
Modified residuei27Phosphoserine1 Publication1
Modified residuei47Phosphothreonine1 Publication1
Modified residuei52Phosphoserine1 Publication1
Modified residuei165Phosphothreonine1 Publication1
Modified residuei168Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated during M and G1 phases.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP54350
PRIDEiP54350

PTM databases

iPTMnetiP54350

Expressioni

Tissue specificityi

Expressed in embryos; expression remains high in the proliferating cells of the central nervous system well after cells in the rest of the embryo have ceased dividing.1 Publication

Developmental stagei

Expressed both maternally and zygotically during postblastoderm divisions of embryogenesis.1 Publication

Gene expression databases

BgeeiFBgn0011737
ExpressionAtlasiP54350 differential
GenevisibleiP54350 DM

Interactioni

Protein-protein interaction databases

BioGridi6011620 interactors.
DIPiDIP-23536N
IntActiP54350 11 interactors.
STRINGi7227.FBpp0304607

Structurei

3D structure databases

ProteinModelPortaliP54350
SMRiP54350
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini239 – 517Protein kinasePROSITE-ProRule annotationAdd BLAST279

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi122 – 126Poly-Ala5

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0601 Eukaryota
ENOG410XS1M LUCA
GeneTreeiENSGT00530000063230
InParanoidiP54350
KOiK06632
OMAiWNDSCGE
OrthoDBiEOG091G049H
PhylomeDBiP54350

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR017164 Wee1-like_protein_kinase
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PIRSFiPIRSF037281 Wee1-like_protein_kinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 2 hits
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

P54350-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFRQSEHEM SVTSLDSSVE LRSRSPSPQV FNPRKLRFAD DDFDKDTPEG
60 70 80 90 100
ASPQHPLQQR PKLSSGEEQQ LDSKIGKEGG DGDVSMSPPC QKVRALRLFS
110 120 130 140 150
TPATPKTILQ KSTTQCSNHL SAAAAAVNAS RRSDDLFRLS ERPRSLPLHN
160 170 180 190 200
RKLPTQDTAN VNPFTPDSLM AHNKKRCRTQ FGRENLNLNV NAMQKYLLSD
210 220 230 240 250
ACDDDVTEEA GDSMREIHQQ APKRLALHDT NISRFKREFM QVNVIGVGEF
260 270 280 290 300
GVVFQCVNRL DGCIYAIKKS KKPVAGSSFE KRALNEVWAH AVLGKHDNVV
310 320 330 340 350
RYYSAWAEDD HMLIQNEFCD GGSLHARIQD HCLGEAELKI VLMHVIEGLR
360 370 380 390 400
YIHSNDLVHM DLKPENIFST MNPNAHKLVE VQPQQTKDDD GMDSVYEELR
410 420 430 440 450
HSENLVTYKI GDLGHVTSVK EPYVEEGDCR YLPKEILHED YSNLFKADIF
460 470 480 490 500
SLGITLFEAA GGGPLPKNGP EWHNLRDGKV PILPSLSRDF NELIAQMMHP
510 520 530 540 550
YPDKRPTSQS IFSHPILSAV DSKSKLQLGL ELTVEKRKNE ILMNKLREAK
560 570 580 590 600
KQIKLLEQRV NLLAVTNNPD SLDGQRCLRS FTRRMRTPFS SHGKFDSISD

RNKNVITNI
Length:609
Mass (Da):68,809
Last modified:December 1, 2000 - v2
Checksum:i7E371EFDA2A5CD96
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35 – 36KL → NV in AAC46913 (PubMed:8573790).Curated2
Sequence conflicti72D → G in AAC46913 (PubMed:8573790).Curated1
Sequence conflicti275A → R in AAC46913 (PubMed:8573790).Curated1
Sequence conflicti567N → K in AAC46913 (PubMed:8573790).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17223 mRNA Translation: AAC46913.2
AE014134 Genomic DNA Translation: AAF52453.2
AY118942 mRNA Translation: AAM50802.1
RefSeqiNP_001260167.1, NM_001273238.1
NP_477035.1, NM_057687.4
UniGeneiDm.3106

Genome annotation databases

EnsemblMetazoaiFBtr0079371; FBpp0078999; FBgn0011737
FBtr0332329; FBpp0304607; FBgn0011737
GeneIDi33965
KEGGidme:Dmel_CG4488

Similar proteinsi

Entry informationi

Entry nameiWEE1_DROME
AccessioniPrimary (citable) accession number: P54350
Secondary accession number(s): Q9VM70
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 1, 2000
Last modified: March 28, 2018
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome