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P54350

- WEE1_DROME

UniProt

P54350 - WEE1_DROME

Protein

Wee1-like protein kinase

Gene

wee

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Could act as a negative regulator of entry into mitosis (G2 to M transition). This kinase specifically phosphorylates and inactivates cyclin B1-complexed CDC2.1 Publication

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

    Cofactori

    Binds 2 magnesium ions per subunit.By similarity

    Enzyme regulationi

    Negatively regulated by phosphorylation in the M-phase.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei268 – 2681ATPPROSITE-ProRule annotation
    Active sitei361 – 3611Proton acceptorPROSITE-ProRule annotation
    Metal bindingi366 – 3661Magnesium; via carbonyl oxygenBy similarity
    Metal bindingi412 – 4121Magnesium; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi245 – 2539ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    4. protein kinase activity Source: FlyBase
    5. protein tyrosine kinase activity Source: FlyBase

    GO - Biological processi

    1. centrosome localization Source: FlyBase
    2. centrosome separation Source: FlyBase
    3. embryonic development via the syncytial blastoderm Source: FlyBase
    4. mitotic cell cycle Source: FlyBase
    5. mitotic cell cycle checkpoint Source: FlyBase
    6. mitotic nuclear division Source: UniProtKB-KW
    7. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: FlyBase
    8. peptidyl-tyrosine phosphorylation Source: GOC
    9. protein phosphorylation Source: FlyBase
    10. spindle assembly Source: FlyBase

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.10.2. 1994.
    ReactomeiREACT_180258. Factors involved in megakaryocyte development and platelet production.
    REACT_208929. Polo-like kinase mediated events.
    REACT_225636. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
    SignaLinkiP54350.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Wee1-like protein kinase (EC:2.7.10.2)
    Short name:
    Dwee1
    Gene namesi
    Name:wee
    ORF Names:CG4488
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2L

    Organism-specific databases

    FlyBaseiFBgn0011737. wee.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. microtubule associated complex Source: FlyBase
    2. nucleus Source: FlyBase

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 609609Wee1-like protein kinasePRO_0000086814Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei23 – 231Phosphoserine1 Publication
    Modified residuei25 – 251Phosphoserine1 Publication
    Modified residuei27 – 271Phosphoserine1 Publication
    Modified residuei47 – 471Phosphothreonine1 Publication
    Modified residuei52 – 521Phosphoserine1 Publication
    Modified residuei165 – 1651Phosphothreonine1 Publication
    Modified residuei168 – 1681Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylated during M and G1 phases.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP54350.
    PRIDEiP54350.

    Expressioni

    Tissue specificityi

    Expressed in embryos; expression remains high in the proliferating cells of the central nervous system well after cells in the rest of the embryo have ceased dividing.1 Publication

    Developmental stagei

    Expressed both maternally and zygotically during postblastoderm divisions of embryogenesis.1 Publication

    Gene expression databases

    BgeeiP54350.

    Interactioni

    Protein-protein interaction databases

    BioGridi60116. 15 interactions.
    DIPiDIP-23536N.
    IntActiP54350. 2 interactions.
    MINTiMINT-885153.
    STRINGi7227.FBpp0078999.

    Structurei

    3D structure databases

    ProteinModelPortaliP54350.
    SMRiP54350. Positions 214-587.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini239 – 517279Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi122 – 1265Poly-Ala

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00530000063230.
    InParanoidiP54350.
    KOiK06632.
    OMAiCIYAIKK.
    OrthoDBiEOG7N63M9.
    PhylomeDBiP54350.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR017164. Wee1-like_protein_kinase.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
    SUPFAMiSSF56112. SSF56112. 2 hits.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P54350-1 [UniParc]FASTAAdd to Basket

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    MAFRQSEHEM SVTSLDSSVE LRSRSPSPQV FNPRKLRFAD DDFDKDTPEG    50
    ASPQHPLQQR PKLSSGEEQQ LDSKIGKEGG DGDVSMSPPC QKVRALRLFS 100
    TPATPKTILQ KSTTQCSNHL SAAAAAVNAS RRSDDLFRLS ERPRSLPLHN 150
    RKLPTQDTAN VNPFTPDSLM AHNKKRCRTQ FGRENLNLNV NAMQKYLLSD 200
    ACDDDVTEEA GDSMREIHQQ APKRLALHDT NISRFKREFM QVNVIGVGEF 250
    GVVFQCVNRL DGCIYAIKKS KKPVAGSSFE KRALNEVWAH AVLGKHDNVV 300
    RYYSAWAEDD HMLIQNEFCD GGSLHARIQD HCLGEAELKI VLMHVIEGLR 350
    YIHSNDLVHM DLKPENIFST MNPNAHKLVE VQPQQTKDDD GMDSVYEELR 400
    HSENLVTYKI GDLGHVTSVK EPYVEEGDCR YLPKEILHED YSNLFKADIF 450
    SLGITLFEAA GGGPLPKNGP EWHNLRDGKV PILPSLSRDF NELIAQMMHP 500
    YPDKRPTSQS IFSHPILSAV DSKSKLQLGL ELTVEKRKNE ILMNKLREAK 550
    KQIKLLEQRV NLLAVTNNPD SLDGQRCLRS FTRRMRTPFS SHGKFDSISD 600
    RNKNVITNI 609
    Length:609
    Mass (Da):68,809
    Last modified:December 1, 2000 - v2
    Checksum:i7E371EFDA2A5CD96
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti35 – 362KL → NV in AAC46913. (PubMed:8573790)Curated
    Sequence conflicti72 – 721D → G in AAC46913. (PubMed:8573790)Curated
    Sequence conflicti275 – 2751A → R in AAC46913. (PubMed:8573790)Curated
    Sequence conflicti567 – 5671N → K in AAC46913. (PubMed:8573790)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17223 mRNA. Translation: AAC46913.2.
    AE014134 Genomic DNA. Translation: AAF52453.2.
    AY118942 mRNA. Translation: AAM50802.1.
    RefSeqiNP_001260167.1. NM_001273238.1.
    NP_477035.1. NM_057687.4.
    UniGeneiDm.3106.

    Genome annotation databases

    EnsemblMetazoaiFBtr0079371; FBpp0078999; FBgn0011737.
    FBtr0332329; FBpp0304607; FBgn0011737.
    GeneIDi33965.
    KEGGidme:Dmel_CG4488.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17223 mRNA. Translation: AAC46913.2 .
    AE014134 Genomic DNA. Translation: AAF52453.2 .
    AY118942 mRNA. Translation: AAM50802.1 .
    RefSeqi NP_001260167.1. NM_001273238.1.
    NP_477035.1. NM_057687.4.
    UniGenei Dm.3106.

    3D structure databases

    ProteinModelPortali P54350.
    SMRi P54350. Positions 214-587.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 60116. 15 interactions.
    DIPi DIP-23536N.
    IntActi P54350. 2 interactions.
    MINTi MINT-885153.
    STRINGi 7227.FBpp0078999.

    Proteomic databases

    PaxDbi P54350.
    PRIDEi P54350.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0079371 ; FBpp0078999 ; FBgn0011737 .
    FBtr0332329 ; FBpp0304607 ; FBgn0011737 .
    GeneIDi 33965.
    KEGGi dme:Dmel_CG4488.

    Organism-specific databases

    CTDi 33965.
    FlyBasei FBgn0011737. wee.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00530000063230.
    InParanoidi P54350.
    KOi K06632.
    OMAi CIYAIKK.
    OrthoDBi EOG7N63M9.
    PhylomeDBi P54350.

    Enzyme and pathway databases

    BRENDAi 2.7.10.2. 1994.
    Reactomei REACT_180258. Factors involved in megakaryocyte development and platelet production.
    REACT_208929. Polo-like kinase mediated events.
    REACT_225636. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
    SignaLinki P54350.

    Miscellaneous databases

    GenomeRNAii 33965.
    NextBioi 786174.
    PROi P54350.

    Gene expression databases

    Bgeei P54350.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR017164. Wee1-like_protein_kinase.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037281. Wee1-like_protein_kinase. 1 hit.
    SUPFAMi SSF56112. SSF56112. 2 hits.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Drosophila Wee1 kinase rescues fission yeast from mitotic catastrophe and phosphorylates Drosophila Cdc2 in vitro."
      Campbell S.D., Sprenger F., Edgar B.A., O'Farrell P.H.
      Mol. Biol. Cell 6:1333-1347(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    2. Campbell S.D.
      Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO C-TERMINUS.
    3. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    4. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.
    6. "Phosphoproteome analysis of Drosophila melanogaster embryos."
      Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
      J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23; SER-25; SER-27; THR-47; SER-52; THR-165 AND SER-168, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Embryo.

    Entry informationi

    Entry nameiWEE1_DROME
    AccessioniPrimary (citable) accession number: P54350
    Secondary accession number(s): Q9VM70
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 132 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3