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Protein

Interleukin-12 subunit alpha

Gene

IL12A

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.By similarity

GO - Molecular functioni

  • cytokine activity Source: GO_Central
  • interleukin-12 receptor binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Growth factor

Enzyme and pathway databases

ReactomeiR-BTA-449147. Signaling by Interleukins.
R-BTA-6788467. IL-6-type cytokine receptor ligand interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-12 subunit alpha
Short name:
IL-12A
Alternative name(s):
Cytotoxic lymphocyte maturation factor 35 kDa subunit
Short name:
CLMF p35
IL-12 subunit p35
Gene namesi
Name:IL12A
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: Ensembl
  • extracellular space Source: UniProtKB
  • interleukin-12 complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525By similarityAdd
BLAST
Chaini26 – 221196Interleukin-12 subunit alphaPRO_0000015597Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi66 ↔ 198By similarity
Disulfide bondi87 ↔ 125By similarity
Glycosylationi95 – 951N-linked (GlcNAc...)Sequence analysis
Disulfide bondi98 – 98Interchain (with C-200 in IL12B)By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP54349.
PRIDEiP54349.

Interactioni

Subunit structurei

Heterodimer with IL12B; disulfide-linked. The heterodimer is known as interleukin IL-12.

GO - Molecular functioni

  • cytokine activity Source: GO_Central
  • interleukin-12 receptor binding Source: UniProtKB

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000020153.

Structurei

3D structure databases

ProteinModelPortaliP54349.
SMRiP54349. Positions 44-221.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IL-6 superfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IVIR. Eukaryota.
ENOG410ZGHJ. LUCA.
GeneTreeiENSGT00390000016906.
HOGENOMiHOG000113026.
HOVERGENiHBG063691.
InParanoidiP54349.
KOiK05406.
OMAiNFNSETV.
OrthoDBiEOG7VDXQX.
TreeFamiTF330814.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR004281. IL-12_alpha.
[Graphical view]
PANTHERiPTHR10523. PTHR10523. 1 hit.
PfamiPF03039. IL12. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54349-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCPLRSLLLI STLVLLHHLP HLSLGRSLPT TTASPGRSCL DYSQNLLRAV
60 70 80 90 100
SNTLQKARQT LEFYSCTSEE IDHEDITKDK TSTVEACLPL ELATNESCLA
110 120 130 140 150
SRETSFITNG HCLASGKTSF MTTLCLRSIY EDLKMYHVEF QAMNAKLLMD
160 170 180 190 200
PKRQIFLDQN MLAAIAELMQ ALNFDSETVP QKPSLKELDF YKTKVKLCIL
210 220
LHAFRIRAVT IDRMMSYLSS S
Length:221
Mass (Da):24,944
Last modified:October 1, 1996 - v1
Checksum:i0462DEE9DD33BC90
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14416 mRNA. Translation: AAA85810.1.
EU276075 mRNA. Translation: ABX72073.1.
RefSeqiNP_776780.1. NM_174355.2.
UniGeneiBt.524.

Genome annotation databases

EnsembliENSBTAT00000020153; ENSBTAP00000020153; ENSBTAG00000015150.
GeneIDi281856.
KEGGibta:281856.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14416 mRNA. Translation: AAA85810.1.
EU276075 mRNA. Translation: ABX72073.1.
RefSeqiNP_776780.1. NM_174355.2.
UniGeneiBt.524.

3D structure databases

ProteinModelPortaliP54349.
SMRiP54349. Positions 44-221.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000020153.

Proteomic databases

PaxDbiP54349.
PRIDEiP54349.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000020153; ENSBTAP00000020153; ENSBTAG00000015150.
GeneIDi281856.
KEGGibta:281856.

Organism-specific databases

CTDi3592.

Phylogenomic databases

eggNOGiENOG410IVIR. Eukaryota.
ENOG410ZGHJ. LUCA.
GeneTreeiENSGT00390000016906.
HOGENOMiHOG000113026.
HOVERGENiHBG063691.
InParanoidiP54349.
KOiK05406.
OMAiNFNSETV.
OrthoDBiEOG7VDXQX.
TreeFamiTF330814.

Enzyme and pathway databases

ReactomeiR-BTA-449147. Signaling by Interleukins.
R-BTA-6788467. IL-6-type cytokine receptor ligand interactions.

Miscellaneous databases

NextBioi20805760.

Family and domain databases

Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR004281. IL-12_alpha.
[Graphical view]
PANTHERiPTHR10523. PTHR10523. 1 hit.
PfamiPF03039. IL12. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Enzymatic amplification and molecular cloning of cDNA encoding the small and large subunits of bovine interleukin 12."
    Zarlenga D.S., Canals A., Aschenbrenner R.A., Gasbarre L.C.
    Biochim. Biophys. Acta 1270:215-217(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Holstein.
    Tissue: Lymphoid tissue.
  2. "U.S. veterinary immune reagent network: expressed bovine gene sequences."
    U.S. Veterinary Immune Reagent Network
    Hudgens T., Tompkins D., Baldwin C.L.
    Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Belted Galloway.
    Tissue: Peripheral blood.

Entry informationi

Entry nameiIL12A_BOVIN
AccessioniPrimary (citable) accession number: P54349
Secondary accession number(s): A9QWR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 17, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.