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Protein

Elastin

Gene

Eln

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.1 Publication

GO - Molecular functioni

  • extracellular matrix binding Source: MGI
  • extracellular matrix structural constituent Source: InterPro

GO - Biological processi

  • extracellular matrix organization Source: MGI
  • regulation of actin filament polymerization Source: MGI
  • skeletal muscle tissue development Source: MGI
  • stress fiber assembly Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-1474228. Degradation of the extracellular matrix.
R-MMU-1566948. Elastic fibre formation.
R-MMU-2129379. Molecules associated with elastic fibres.

Names & Taxonomyi

Protein namesi
Recommended name:
Elastin
Alternative name(s):
Tropoelastin
Gene namesi
Name:Eln
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:95317. Eln.

Subcellular locationi

  • Secretedextracellular spaceextracellular matrix By similarity

  • Note: Extracellular matrix of elastic fibers.By similarity

GO - Cellular componenti

  • elastic fiber Source: MGI
  • mitochondrion Source: MGI
  • proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27By similarityAdd BLAST27
ChainiPRO_000002116428 – 860ElastinAdd BLAST833

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei354-hydroxyprolineBy similarity1
Modified residuei724-hydroxyprolineBy similarity1
Modified residuei84HydroxyprolineBy similarity1
Modified residuei1054-hydroxyprolineBy similarity1
Modified residuei123AllysineBy similarity1
Modified residuei127AllysineBy similarity1
Modified residuei2174-hydroxyprolineBy similarity1
Modified residuei2304-hydroxyprolineBy similarity1
Modified residuei2334-hydroxyprolineBy similarity1
Modified residuei2534-hydroxyprolineBy similarity1
Modified residuei299AllysineBy similarity1
Modified residuei318AllysineBy similarity1
Modified residuei321AllysineBy similarity1
Modified residuei3464-hydroxyprolineBy similarity1
Modified residuei368AllysineBy similarity1
Modified residuei371AllysineBy similarity1
Modified residuei383HydroxyprolineBy similarity1
Modified residuei3994-hydroxyprolineBy similarity1
Modified residuei4054-hydroxyprolineBy similarity1
Modified residuei410HydroxyprolineBy similarity1
Modified residuei415HydroxyprolineBy similarity1
Modified residuei431AllysineBy similarity1
Modified residuei435AllysineBy similarity1
Modified residuei438AllysineBy similarity1
Modified residuei484AllysineBy similarity1
Modified residuei4984-hydroxyprolineBy similarity1
Modified residuei5194-hydroxyprolineBy similarity1
Modified residuei534AllysineBy similarity1
Modified residuei595AllysineBy similarity1
Modified residuei599AllysineBy similarity1
Modified residuei603AllysineBy similarity1
Modified residuei6174-hydroxyprolineBy similarity1
Modified residuei6264-hydroxyprolineBy similarity1
Modified residuei6444-hydroxyprolineBy similarity1
Modified residuei6534-hydroxyprolineBy similarity1
Modified residuei6614-hydroxyprolineBy similarity1
Modified residuei668AllysineBy similarity1
Modified residuei671AllysineBy similarity1
Modified residuei7024-hydroxyprolineBy similarity1
Modified residuei719AllysineBy similarity1
Modified residuei723AllysineBy similarity1
Modified residuei783AllysineBy similarity1
Modified residuei786AllysineBy similarity1
Modified residuei8324-hydroxyprolineBy similarity1
Disulfide bondi850 ↔ 855By similarity

Post-translational modificationi

Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.By similarity

Keywords - PTMi

Disulfide bond, Hydroxylation

Proteomic databases

MaxQBiP54320.
PaxDbiP54320.
PRIDEiP54320.

PTM databases

PhosphoSitePlusiP54320.

Expressioni

Gene expression databases

BgeeiENSMUSG00000029675.
CleanExiMM_ELN.
ExpressionAtlasiP54320. baseline and differential.
GenevisibleiP54320. MM.

Interactioni

Subunit structurei

The polymeric elastin chains are cross-linked together into an extensible 3D network. Forms a ternary complex with BGN and MFAP2. Interacts with MFAP2 via divalent cations (calcium > magnesium > manganese) in a dose-dependent and saturating manner. Interacts with FBLN5 and FBN1. Forms a ternary complex with FBN1 and FBLN2 or FBLN5. Interacts with MFAP4 in a Ca (2+)-dependent manner; this interaction promotes ELN self-assembly.By similarity

Protein-protein interaction databases

BioGridi199433. 2 interactors.
IntActiP54320. 1 interactor.
STRINGi10090.ENSMUSP00000015138.

Structurei

3D structure databases

ProteinModelPortaliP54320.
SMRiP54320.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the elastin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IZT8. Eukaryota.
ENOG4111817. LUCA.
GeneTreeiENSGT00730000111510.
InParanoidiP54320.
KOiK14211.
OMAiQGGACLG.

Family and domain databases

InterProiIPR003979. Tropoelastin.
[Graphical view]
PRINTSiPR01500. TROPOELASTIN.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54320-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGLTAVVPQ PGVLLILLLN LLHPAQPGGV PGAVPGGLPG GVPGGVYYPG
60 70 80 90 100
AGIGGLGGGG GALGPGGKPP KPGAGLLGTF GAGPGGLGGA GPGAGLGAFP
110 120 130 140 150
AGTFPGAGAL VPGGAAGAAA AYKAAAKAGA GLGGVGGVPG GVGVGGVPGG
160 170 180 190 200
VGVGGVPGGV GVGGVPGGVG GIGGIGGLGV STGAVVPQVG AGIGAGGKPG
210 220 230 240 250
KVPGVGLPGV YPGGVLPGTG ARFPGVGVLP GVPTGTGVKA KAPGGGGAFA
260 270 280 290 300
GIPGVGPFGG QQPGVPLGYP IKAPKLPGGY GLPYTNGKLP YGVAGAGGKA
310 320 330 340 350
GYPTGTGVGS QAAAAAAKAA KYGAGGAGVL PGVGGGGIPG GAGAIPGIGG
360 370 380 390 400
IAGAGTPAAA AAAKAAAKAA KYGAAGGLVP GGPGVRLPGA GIPGVGGIPG
410 420 430 440 450
VGGIPGVGGP GIGGPGIVGG PGAVSPAAAA KAAAKAAKYG ARGGVGIPTY
460 470 480 490 500
GVGAGGFPGY GVGAGAGLGG ASPAAAAAAA KAAKYGAGGA GALGGLVPGA
510 520 530 540 550
VPGALPGAVP AVPGAGGVPG AGTPAAAAAA AAAKAAAKAG LGPGVGGVPG
560 570 580 590 600
GVGVGGIPGG VGVGGVPGGV GPGGVTGIGA GPGGLGGAGS PAAAKSAAKA
610 620 630 640 650
AAKAQYRAAA GLGAGVPGFG AGAGVPGFGA GAGVPGFGAG AGVPGFGAGA
660 670 680 690 700
GVPGFGAGAV PGSLAASKAA KYGAAGGLGG PGGLGGPGGL GGPGGLGGAG
710 720 730 740 750
VPGRVAGAAP PAAAAAAAKA AAKAAQYGLG GAGGLGAGGL GAGGLGAGGL
760 770 780 790 800
GAGGLGAGGL GAGGLGAGGL GAGGGVSPAA AAKAAKYGAA GLGGVLGARP
810 820 830 840 850
FPGGGVAARP GFGLSPIYPG GGAGGLGVGG KPPKPYGGAL GALGYQGGGC
860
FGKSCGRKRK
Length:860
Mass (Da):71,938
Last modified:July 27, 2011 - v2
Checksum:i7C340F2FFFDC92E5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti250A → S in AAA80155 (PubMed:7829060).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08210 mRNA. Translation: AAA80155.1.
AK041860 mRNA. Translation: BAC31084.1.
CH466529 Genomic DNA. Translation: EDL19414.1.
BC051649 mRNA. Translation: AAH51649.1.
CCDSiCCDS19725.1.
PIRiA55721. EAMS.
RefSeqiNP_031951.2. NM_007925.4.
UniGeneiMm.275320.
Mm.404771.

Genome annotation databases

EnsembliENSMUST00000015138; ENSMUSP00000015138; ENSMUSG00000029675.
GeneIDi13717.
KEGGimmu:13717.
UCSCiuc008zwv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08210 mRNA. Translation: AAA80155.1.
AK041860 mRNA. Translation: BAC31084.1.
CH466529 Genomic DNA. Translation: EDL19414.1.
BC051649 mRNA. Translation: AAH51649.1.
CCDSiCCDS19725.1.
PIRiA55721. EAMS.
RefSeqiNP_031951.2. NM_007925.4.
UniGeneiMm.275320.
Mm.404771.

3D structure databases

ProteinModelPortaliP54320.
SMRiP54320.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199433. 2 interactors.
IntActiP54320. 1 interactor.
STRINGi10090.ENSMUSP00000015138.

PTM databases

PhosphoSitePlusiP54320.

Proteomic databases

MaxQBiP54320.
PaxDbiP54320.
PRIDEiP54320.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015138; ENSMUSP00000015138; ENSMUSG00000029675.
GeneIDi13717.
KEGGimmu:13717.
UCSCiuc008zwv.1. mouse.

Organism-specific databases

CTDi2006.
MGIiMGI:95317. Eln.

Phylogenomic databases

eggNOGiENOG410IZT8. Eukaryota.
ENOG4111817. LUCA.
GeneTreeiENSGT00730000111510.
InParanoidiP54320.
KOiK14211.
OMAiQGGACLG.

Enzyme and pathway databases

ReactomeiR-MMU-1474228. Degradation of the extracellular matrix.
R-MMU-1566948. Elastic fibre formation.
R-MMU-2129379. Molecules associated with elastic fibres.

Miscellaneous databases

ChiTaRSiEln. mouse.
PROiP54320.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029675.
CleanExiMM_ELN.
ExpressionAtlasiP54320. baseline and differential.
GenevisibleiP54320. MM.

Family and domain databases

InterProiIPR003979. Tropoelastin.
[Graphical view]
PRINTSiPR01500. TROPOELASTIN.
ProtoNetiSearch...

Entry informationi

Entry nameiELN_MOUSE
AccessioniPrimary (citable) accession number: P54320
Secondary accession number(s): Q8C9L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.