P54315 (LIPR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inactive pancreatic lipase-related protein 1
Short name=PL-RP1

Gene names

Name:	PNLIPRP1
Synonyms:	PLRP1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as inhibitor of dietary triglyceride digestion. Lacks detectable lipase activity towards triglycerides, diglycerides, phosphatidylcholine, galactolipids or cholesterol esters (in vitro) By similarity. Ref.4
Subcellular location	Secreted Ref.1 Ref.4.
Tissue specificity	Pancreas. Ref.1
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family. Contains 1 PLAT domain.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Ligand	Calcium Metal-binding
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Traceable author statement Ref.1. Source: ProtInc
Molecular_function	calcium ion binding Inferred from sequence or structural similarity. Source: UniProtKB triglyceride lipase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P54315-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P54315-2)

The sequence of this isoform differs from the canonical sequence as follows:
     111-186: KLFEVEEVNC...AGEAGSKTPG → VGASSDPCGQ...EKYSYYLISG
     187-467: Missing.

Isoform 3 (identifier: P54315-3)

The sequence of this isoform differs from the canonical sequence as follows:
     111-117: KLFEVEE → PGASPRA
     118-467: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 17

17

Potential

Chain

18 – 467

450

Inactive pancreatic lipase-related protein 1

PRO_0000017790

Regions

Domain

356 – 467

112

PLAT

Sites

Active site

171

1

Nucleophile

Active site

194

1

Charge relay system

Active site

281

1

Charge relay system

Metal binding

205

1

Calcium; via carbonyl oxygen

Metal binding

208

1

Calcium; via carbonyl oxygen

Metal binding

210

1

Calcium

Metal binding

213

1

Calcium

Amino acid modifications

Disulfide bond

21 ↔ 27

Ref.5

Disulfide bond

109 ↔ 120

Ref.5

Disulfide bond

255 ↔ 279

Ref.5

Disulfide bond

303 ↔ 314

Ref.5

Disulfide bond

317 ↔ 322

Ref.5

Disulfide bond

451 ↔ 467

Ref.5

Natural variations

Alternative sequence

111 – 186

76

KLFEV…SKTPG → VGASSDPCGQLRPTLLLTSL HHFMHSRNLYILGNFMQLKC FSSQKLKCLSMFPHYICTLK QPHLLLEKYSYYLISG in isoform 2.

VSP_014097

Alternative sequence

111 – 117

7

KLFEVEE → PGASPRA in isoform 3.

VSP_014098

Alternative sequence

118 – 467

350

Missing in isoform 3.

VSP_014099

Alternative sequence

187 – 467

281

Missing in isoform 2.

VSP_014100

Natural variant

61

1

N → D.
Corresponds to variant rs11197744 [ dbSNP | Ensembl ].

VAR_049820

Natural variant

129

1

S → C in a breast cancer sample; somatic mutation. Ref.6

VAR_036379

Natural variant

271

1

A → V.
Corresponds to variant rs2305205 [ dbSNP | Ensembl ].

VAR_022082

Natural variant

414

1

E → D. Ref.3
Corresponds to variant rs2305204 [ dbSNP | Ensembl ].

VAR_022659

Natural variant

461

1

L → P.
Corresponds to variant rs1049125 [ dbSNP | Ensembl ].

VAR_014915

Secondary structure

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467

Helix Strand Turn

Details...

Beta strand

19 – 22

4

Turn

23 – 25

3

Beta strand

26 – 30

5

Turn

32 – 34

3

Beta strand

35 – 40

6

Helix

49 – 52

4

Beta strand

55 – 63

9

Beta strand

68 – 71

4

Helix

76 – 80

5

Beta strand

87 – 93

7

Helix

104 – 115

12

Beta strand

118 – 124

7

Helix

126 – 129

4

Helix

133 – 158

26

Helix

162 – 164

3

Beta strand

165 – 170

6

Helix

173 – 182

10

Beta strand

189 – 194

6

Turn

198 – 202

5

Turn

205 – 207

3

Helix

211 – 213

3

Beta strand

214 – 220

7

Helix

227 – 230

4

Beta strand

240 – 246

7

Beta strand

249 – 251

3

Helix

266 – 270

5

Helix

279 – 293

15

Turn

295 – 298

4

Helix

306 – 310

5

Beta strand

324 – 326

3

Helix

327 – 331

5

Beta strand

341 – 345

5

Beta strand

349 – 352

4

Beta strand

356 – 367

12

Beta strand

369 – 379

11

Beta strand

387 – 394

8

Beta strand

399 – 408

10

Beta strand

412 – 421

10

Beta strand

432 – 441

10

Beta strand

447 – 451

5

Beta strand

462 – 466

5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 2781EA1E22E39E78	FASTA	467	51,848
	10 20 30 40 50 60 MLIFWTITLF LLGAAKGKEV CYEDLGCFSD TEPWGGTAIR PLKILPWSPE KIGTRFLLYT 70 80 90 100 110 120 NENPNNFQIL LLSDPSTIEA SNFQMDRKTR FIIHGFIDKG DESWVTDMCK KLFEVEEVNC 130 140 150 160 170 180 ICVDWKKGSQ ATYTQAANNV RVVGAQVAQM LDILLTEYSY PPSKVHLIGH SLGAHVAGEA 190 200 210 220 230 240 GSKTPGLSRI TGLDPVEASF ESTPEEVRLD PSDADFVDVI HTDAAPLIPF LGFGTNQQMG 250 260 270 280 290 300 HLDFFPNGGE SMPGCKKNAL SQIVDLDGIW AGTRDFVACN HLRSYKYYLE SILNPDGFAA 310 320 330 340 350 360 YPCTSYKSFE SDKCFPCPDQ GCPQMGHYAD KFAGRTSEEQ QKFFLNTGEA SNFARWRYGV 370 380 390 400 410 420 SITLSGRTAT GQIKVALFGN KGNTHQYSIF RGILKPGSTH SYEFDAKLDV GTIEKVKFLW 430 440 450 460 NNNVINPTLP KVGATKITVQ KGEEKTVYNF CSEDTVREDT LLTLTPC
	Isoform 2 [UniParc]. Checksum: 00AA562CC980CDF0	FASTA	186	21,354
	10 20 30 40 50 60 MLIFWTITLF LLGAAKGKEV CYEDLGCFSD TEPWGGTAIR PLKILPWSPE KIGTRFLLYT 70 80 90 100 110 120 NENPNNFQIL LLSDPSTIEA SNFQMDRKTR FIIHGFIDKG DESWVTDMCK VGASSDPCGQ 130 140 150 160 170 180 LRPTLLLTSL HHFMHSRNLY ILGNFMQLKC FSSQKLKCLS MFPHYICTLK QPHLLLEKYS YYLISG
	Isoform 3 [UniParc]. Checksum: 24E13DBDF751B557	FASTA	117	13,245
	10 20 30 40 50 60 MLIFWTITLF LLGAAKGKEV CYEDLGCFSD TEPWGGTAIR PLKILPWSPE KIGTRFLLYT 70 80 90 100 110 NENPNNFQIL LLSDPSTIEA SNFQMDRKTR FIIHGFIDKG DESWVTDMCK PGASPRA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two novel human pancreatic lipase related proteins, hPLRP1 and hPLRP2. Differences in colipase dependence and in lipase activity." Giller T., Buchwald P., Blum-Kaelin D., Hunziker W. J. Biol. Chem. 267:16509-16516(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ABSENCE OF LIPASE ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Pancreas.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT ASP-414. Tissue: Pancreas.
[4]	"Individual and combined action of pancreatic lipase and pancreatic lipase-related proteins 1 and 2 on native versus homogenized milk fat globules." Berton A., Sebban-Kreuzer C., Rouvellac S., Lopez C., Crenon I. Mol. Nutr. Food Res. 53:1592-1602(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, ABSENCE OF CATALYTIC ACTIVITY.
[5]	"Structure of the human pancreatic lipase-related protein 1." Structural genomics consortium (SGC) Submitted (FEB-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 18-467 IN COMPLEX WITH CALCIUM IONS, DISULFIDE BONDS.
[6]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] CYS-129.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M93283 mRNA. Translation: AAA59532.1.
CR749299 mRNA. Translation: CAH18154.1.
CR749524 mRNA. Translation: CAH18337.1.
BC005233 mRNA. Translation: AAH05233.1.
BC025784 mRNA. Translation: AAH25784.1.

IPI

IPI00005923.
IPI00386645.
IPI00873836.

PIR

A43357.

RefSeq

NP_006220.1. NM_006229.2.

UniGene

Hs.73923.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PPL	X-ray	2.20	A	18-467	[»]

ProteinModelPortal

P54315.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000351695.

PTM databases

PhosphoSite

P54315.

Polymorphism databases

DMDM

1708837.

Proteomic databases

PaxDb

P54315.

PRIDE

P54315.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000358834; ENSP00000351695; ENSG00000187021.
ENST00000442761; ENSP00000400963; ENSG00000187021.
ENST00000528052; ENSP00000433933; ENSG00000187021.
ENST00000572596; ENSP00000458759; ENSG00000262273.
ENST00000572926; ENSP00000461234; ENSG00000262273.

GeneID

5407.

KEGG

hsa:5407.

UCSC

uc001lcn.3. human.
uc001lco.1. human.

Organism-specific databases

CTD

5407.

GeneCards

GC10P118340.

HGNC

HGNC:9156. PNLIPRP1.

MIM

604422. gene.

neXtProt

NX_P54315.

PharmGKB

PA33479.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG40923.

HOGENOM

HOG000038552.

HOVERGEN

HBG003243.

InParanoid

P54315.

KO

K14074.

OMA

KGDESWV.

OrthoDB

EOG4WH8KM.

PhylomeDB

P54315.

Gene expression databases

ArrayExpress

P54315.

Bgee

P54315.

CleanEx

HS_PNLIPRP1.

Genevestigator

P54315.

GermOnline

ENSG00000187021. Homo sapiens.

Family and domain databases

Gene3D

2.60.60.20. 1 hit.

InterPro

IPR000734. Lipase.
IPR008976. Lipase_LipOase.
IPR013818. Lipase_N.
IPR002331. Lipase_panc.
IPR001024. LipOase_LH2.
IPR016272. Lipoprotein_lipase_LIPH.
[Graphical view]

PANTHER

PTHR11610. PTHR11610. 1 hit.
PTHR11610:SF8. PTHR11610:SF8. 1 hit.

Pfam

PF00151. Lipase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]

PIRSF

PIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.

PRINTS

PR00823. PANCLIPASE.
PR00821. TAGLIPASE.

SMART

SM00308. LH2. 1 hit.
[Graphical view]

SUPFAM

SSF49723. Lipase_LipOase. 1 hit.

PROSITE

PS00120. LIPASE_SER. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P54315.

GenomeRNAi

5407.

NextBio

20935.

SOURCE

Search...

Entry information

Entry name

LIPR1_HUMAN

Accession

Primary (citable) accession number: P54315
Secondary accession number(s): Q68D83 , Q68DR6, Q8TAU2, Q9BS82

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 119 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.