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Protein

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2

Gene

Gnb2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

GO - Molecular functioni

  • calcium channel regulator activity Source: RGD
  • signal transducer activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Enzyme and pathway databases

ReactomeiR-RNO-1296041. Activation of G protein gated Potassium channels.
R-RNO-163359. Glucagon signaling in metabolic regulation.
R-RNO-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-RNO-400042. Adrenaline,noradrenaline inhibits insulin secretion.
R-RNO-420092. Glucagon-type ligand receptors.
R-RNO-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-RNO-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2
Alternative name(s):
G protein subunit beta-2
Transducin beta chain 2
Gene namesi
Name:Gnb2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi69319. Gnb2.

Subcellular locationi

GO - Cellular componenti

  • cell body Source: RGD
  • extracellular exosome Source: Ensembl
  • extracellular space Source: Ensembl
  • focal adhesion Source: Ensembl
  • lysosomal membrane Source: Ensembl
  • myelin sheath Source: Ensembl
  • perinuclear region of cytoplasm Source: UniProtKB
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 340339Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2PRO_0000127697Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei239 – 2391PhosphotyrosineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP54313.
PRIDEiP54313.

2D gel databases

World-2DPAGE0004:P54313.

PTM databases

iPTMnetiP54313.
PhosphoSiteiP54313.

Expressioni

Gene expression databases

GenevisibleiP54313. RN.

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18 and RASD2 (By similarity). Interacts with ATXN10 (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-61095N.
MINTiMINT-4569271.
STRINGi10116.ENSRNOP00000001911.

Structurei

3D structure databases

ProteinModelPortaliP54313.
SMRiP54313. Positions 1-340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati53 – 8331WD 1Add
BLAST
Repeati95 – 12531WD 2Add
BLAST
Repeati141 – 17030WD 3Add
BLAST
Repeati182 – 21231WD 4Add
BLAST
Repeati224 – 25431WD 5Add
BLAST
Repeati268 – 29831WD 6Add
BLAST
Repeati310 – 34031WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0286. Eukaryota.
ENOG410XQUX. LUCA.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP54313.
KOiK04537.
OMAiDDGMAVC.
OrthoDBiEOG7GN2N5.
PhylomeDBiP54313.
TreeFamiTF106149.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54313-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELEQLRQE AEQLRNQIRD ARKACGDSTL TQITAGLDPV GRIQMRTRRT
60 70 80 90 100
LRGHLAKIYA MHWGTDSRLL VSASQDGKLI IWDSYTTNKV HAIPLRSSWV
110 120 130 140 150
MTCAYAPSGN FVACGGLDNI CSIYSLKTRE GNVRVSRELP GHTGYLSCCR
160 170 180 190 200
FLDDNQIITS SGDTTCALWD IETGQQTVGF AGHSGDVMSL SLAPDGRTFV
210 220 230 240 250
SGACDASIKL WDVRDSMCRQ TFIGHESDIN AVAFFPNGYA FTTGSDDATC
260 270 280 290 300
RLFDLRADQE LLMYSHDNII CGITSVAFSR SGRLLLAGYD DFNCNIWDAM
310 320 330 340
KGDRAGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLKIWN
Length:340
Mass (Da):37,331
Last modified:January 23, 2007 - v4
Checksum:i5D08FFA240ADEEE6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 351A → D in AAC72248 (Ref. 1) Curated
Sequence conflicti60 – 601A → D in AAC72248 (Ref. 1) Curated
Sequence conflicti74 – 741S → A in AAC72248 (Ref. 1) Curated
Sequence conflicti92 – 921A → V in AAC72248 (Ref. 1) Curated
Sequence conflicti101 – 1011M → K in AAC72248 (Ref. 1) Curated
Sequence conflicti120 – 1201I → N in AAC72248 (Ref. 1) Curated
Sequence conflicti137 – 1382RE → KK in AAC72248 (Ref. 1) Curated
Sequence conflicti179 – 1791G → D in AAC72248 (Ref. 1) Curated
Sequence conflicti215 – 2151D → E in AAC72248 (Ref. 1) Curated
Sequence conflicti233 – 2331A → V in AAC72248 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34959 mRNA. Translation: AAC72248.1.
AF277892 mRNA. Translation: AAF82123.1.
BC065579 mRNA. Translation: AAH65579.1.
RefSeqiNP_112299.1. NM_031037.2.
UniGeneiRn.1155.

Genome annotation databases

EnsembliENSRNOT00000001911; ENSRNOP00000001911; ENSRNOG00000001409.
GeneIDi81667.
KEGGirno:81667.
UCSCiRGD:69319. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34959 mRNA. Translation: AAC72248.1.
AF277892 mRNA. Translation: AAF82123.1.
BC065579 mRNA. Translation: AAH65579.1.
RefSeqiNP_112299.1. NM_031037.2.
UniGeneiRn.1155.

3D structure databases

ProteinModelPortaliP54313.
SMRiP54313. Positions 1-340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61095N.
MINTiMINT-4569271.
STRINGi10116.ENSRNOP00000001911.

PTM databases

iPTMnetiP54313.
PhosphoSiteiP54313.

2D gel databases

World-2DPAGE0004:P54313.

Proteomic databases

PaxDbiP54313.
PRIDEiP54313.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001911; ENSRNOP00000001911; ENSRNOG00000001409.
GeneIDi81667.
KEGGirno:81667.
UCSCiRGD:69319. rat.

Organism-specific databases

CTDi2783.
RGDi69319. Gnb2.

Phylogenomic databases

eggNOGiKOG0286. Eukaryota.
ENOG410XQUX. LUCA.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP54313.
KOiK04537.
OMAiDDGMAVC.
OrthoDBiEOG7GN2N5.
PhylomeDBiP54313.
TreeFamiTF106149.

Enzyme and pathway databases

ReactomeiR-RNO-1296041. Activation of G protein gated Potassium channels.
R-RNO-163359. Glucagon signaling in metabolic regulation.
R-RNO-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-RNO-400042. Adrenaline,noradrenaline inhibits insulin secretion.
R-RNO-420092. Glucagon-type ligand receptors.
R-RNO-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-RNO-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Miscellaneous databases

NextBioi615252.
PROiP54313.

Gene expression databases

GenevisibleiP54313. RN.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Kuroda S., Tokunaga C., Konishi H., Kikkawa U.
    Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Differential modulation of N-type 1B and P/Q-type 1A calcium channels by different G protein subunit isoforms."
    Arnot M.I., Stotz S.C., Jarvis S.E., Zamponi G.W.
    J. Physiol. (Lond.) 527:203-212(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  4. Bienvenut W.V., von Kriegsheim A., Kolch W.
    Submitted (JUN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-15; 69-78 AND 198-209, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Fibroblast.
  5. Lubec G., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 58-78 AND 138-150, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.

Entry informationi

Entry nameiGBB2_RAT
AccessioniPrimary (citable) accession number: P54313
Secondary accession number(s): Q71SU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 128 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.