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Protein

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2

Gene

Gnb2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

GO - Molecular functioni

  • calcium channel regulator activity Source: RGD
  • signal transducer activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Enzyme and pathway databases

ReactomeiR-RNO-1296041. Activation of G protein gated Potassium channels.
R-RNO-163359. Glucagon signaling in metabolic regulation.
R-RNO-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-RNO-400042. Adrenaline,noradrenaline inhibits insulin secretion.
R-RNO-420092. Glucagon-type ligand receptors.
R-RNO-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-RNO-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.
R-RNO-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2
Alternative name(s):
G protein subunit beta-2
Transducin beta chain 2
Gene namesi
Name:Gnb2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi69319. Gnb2.

Subcellular locationi

GO - Cellular componenti

  • cell body Source: RGD
  • extracellular exosome Source: Ensembl
  • extracellular space Source: Ensembl
  • focal adhesion Source: Ensembl
  • lysosomal membrane Source: Ensembl
  • myelin sheath Source: Ensembl
  • perinuclear region of cytoplasm Source: UniProtKB
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001276972 – 340Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2Add BLAST339

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei239PhosphotyrosineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP54313.
PRIDEiP54313.

2D gel databases

World-2DPAGE0004:P54313.

PTM databases

iPTMnetiP54313.
PhosphoSitePlusiP54313.

Expressioni

Gene expression databases

BgeeiENSRNOG00000001409.
GenevisibleiP54313. RN.

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18 and RASD2. Interacts with ATXN10. Interacts with SCN8A.By similarity

Protein-protein interaction databases

DIPiDIP-61095N.
MINTiMINT-4569271.
STRINGi10116.ENSRNOP00000001911.

Structurei

3D structure databases

ProteinModelPortaliP54313.
SMRiP54313.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati53 – 83WD 1Add BLAST31
Repeati95 – 125WD 2Add BLAST31
Repeati141 – 170WD 3Add BLAST30
Repeati182 – 212WD 4Add BLAST31
Repeati224 – 254WD 5Add BLAST31
Repeati268 – 298WD 6Add BLAST31
Repeati310 – 340WD 7Add BLAST31

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0286. Eukaryota.
ENOG410XQUX. LUCA.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP54313.
KOiK04537.
OMAiEPVCGIT.
OrthoDBiEOG091G0A7T.
PhylomeDBiP54313.
TreeFamiTF106149.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54313-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELEQLRQE AEQLRNQIRD ARKACGDSTL TQITAGLDPV GRIQMRTRRT
60 70 80 90 100
LRGHLAKIYA MHWGTDSRLL VSASQDGKLI IWDSYTTNKV HAIPLRSSWV
110 120 130 140 150
MTCAYAPSGN FVACGGLDNI CSIYSLKTRE GNVRVSRELP GHTGYLSCCR
160 170 180 190 200
FLDDNQIITS SGDTTCALWD IETGQQTVGF AGHSGDVMSL SLAPDGRTFV
210 220 230 240 250
SGACDASIKL WDVRDSMCRQ TFIGHESDIN AVAFFPNGYA FTTGSDDATC
260 270 280 290 300
RLFDLRADQE LLMYSHDNII CGITSVAFSR SGRLLLAGYD DFNCNIWDAM
310 320 330 340
KGDRAGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLKIWN
Length:340
Mass (Da):37,331
Last modified:January 23, 2007 - v4
Checksum:i5D08FFA240ADEEE6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35A → D in AAC72248 (Ref. 1) Curated1
Sequence conflicti60A → D in AAC72248 (Ref. 1) Curated1
Sequence conflicti74S → A in AAC72248 (Ref. 1) Curated1
Sequence conflicti92A → V in AAC72248 (Ref. 1) Curated1
Sequence conflicti101M → K in AAC72248 (Ref. 1) Curated1
Sequence conflicti120I → N in AAC72248 (Ref. 1) Curated1
Sequence conflicti137 – 138RE → KK in AAC72248 (Ref. 1) Curated2
Sequence conflicti179G → D in AAC72248 (Ref. 1) Curated1
Sequence conflicti215D → E in AAC72248 (Ref. 1) Curated1
Sequence conflicti233A → V in AAC72248 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34959 mRNA. Translation: AAC72248.1.
AF277892 mRNA. Translation: AAF82123.1.
BC065579 mRNA. Translation: AAH65579.1.
RefSeqiNP_112299.1. NM_031037.2.
UniGeneiRn.1155.

Genome annotation databases

EnsembliENSRNOT00000001911; ENSRNOP00000001911; ENSRNOG00000001409.
GeneIDi81667.
KEGGirno:81667.
UCSCiRGD:69319. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34959 mRNA. Translation: AAC72248.1.
AF277892 mRNA. Translation: AAF82123.1.
BC065579 mRNA. Translation: AAH65579.1.
RefSeqiNP_112299.1. NM_031037.2.
UniGeneiRn.1155.

3D structure databases

ProteinModelPortaliP54313.
SMRiP54313.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61095N.
MINTiMINT-4569271.
STRINGi10116.ENSRNOP00000001911.

PTM databases

iPTMnetiP54313.
PhosphoSitePlusiP54313.

2D gel databases

World-2DPAGE0004:P54313.

Proteomic databases

PaxDbiP54313.
PRIDEiP54313.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001911; ENSRNOP00000001911; ENSRNOG00000001409.
GeneIDi81667.
KEGGirno:81667.
UCSCiRGD:69319. rat.

Organism-specific databases

CTDi2783.
RGDi69319. Gnb2.

Phylogenomic databases

eggNOGiKOG0286. Eukaryota.
ENOG410XQUX. LUCA.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP54313.
KOiK04537.
OMAiEPVCGIT.
OrthoDBiEOG091G0A7T.
PhylomeDBiP54313.
TreeFamiTF106149.

Enzyme and pathway databases

ReactomeiR-RNO-1296041. Activation of G protein gated Potassium channels.
R-RNO-163359. Glucagon signaling in metabolic regulation.
R-RNO-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-RNO-400042. Adrenaline,noradrenaline inhibits insulin secretion.
R-RNO-420092. Glucagon-type ligand receptors.
R-RNO-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-RNO-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.
R-RNO-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Miscellaneous databases

PROiP54313.

Gene expression databases

BgeeiENSRNOG00000001409.
GenevisibleiP54313. RN.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGBB2_RAT
AccessioniPrimary (citable) accession number: P54313
Secondary accession number(s): Q71SU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 131 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.