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Protein

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Gene

Gnb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

GO - Molecular functioni

  • alkylglycerophosphoethanolamine phosphodiesterase activity Source: MGI
  • GTPase activity Source: Ensembl
  • signal transducer activity Source: UniProtKB-KW
  • spectrin binding Source: MGI

GO - Biological processi

  • cardiac muscle cell apoptotic process Source: MGI
  • cell proliferation Source: Ensembl
  • cellular response to hypoxia Source: MGI
  • G-protein coupled receptor signaling pathway Source: RGD
  • phospholipase C-activating G-protein coupled receptor signaling pathway Source: Ensembl
  • positive regulation of cytosolic calcium ion concentration Source: RGD
  • protein heterotrimerization Source: MGI
  • retina development in camera-type eye Source: Ensembl
  • sensory perception of taste Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Enzyme and pathway databases

ReactomeiR-RNO-1296041. Activation of G protein gated Potassium channels.
R-RNO-163359. Glucagon signaling in metabolic regulation.
R-RNO-202040. G-protein activation.
R-RNO-2485179. Activation of the phototransduction cascade.
R-RNO-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-RNO-381753. Olfactory Signaling Pathway.
R-RNO-392170. ADP signalling through P2Y purinoceptor 12.
R-RNO-392451. G beta:gamma signalling through PI3Kgamma.
R-RNO-392851. Prostacyclin signalling through prostacyclin receptor.
R-RNO-400042. Adrenaline,noradrenaline inhibits insulin secretion.
R-RNO-4086398. Ca2+ pathway.
R-RNO-416476. G alpha (q) signalling events.
R-RNO-416482. G alpha (12/13) signalling events.
R-RNO-418217. G beta:gamma signalling through PLC beta.
R-RNO-418555. G alpha (s) signalling events.
R-RNO-418592. ADP signalling through P2Y purinoceptor 1.
R-RNO-418594. G alpha (i) signalling events.
R-RNO-418597. G alpha (z) signalling events.
R-RNO-420092. Glucagon-type ligand receptors.
R-RNO-428930. Thromboxane signalling through TP receptor.
R-RNO-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-RNO-456926. Thrombin signalling through proteinase activated receptors (PARs).
R-RNO-500657. Presynaptic function of Kainate receptors.
R-RNO-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.
R-RNO-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
Alternative name(s):
Transducin beta chain 1
Gene namesi
Name:Gnb1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi2718. Gnb1.

Subcellular locationi

GO - Cellular componenti

  • cell body Source: RGD
  • dendrite Source: RGD
  • extracellular exosome Source: Ensembl
  • heterotrimeric G-protein complex Source: RGD
  • lysosomal membrane Source: Ensembl
  • myelin sheath Source: Ensembl
  • photoreceptor inner segment Source: RGD
  • photoreceptor outer segment membrane Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001276902 – 340Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1Add BLAST339

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei2PhosphoserineCombined sources1
Modified residuei266PhosphohistidineBy similarity1

Post-translational modificationi

Phosphorylation at His-266 by NDKB contributes to G protein activation by increasing the high energetic phosphate transfer onto GDP.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP54311.
PRIDEiP54311.

PTM databases

iPTMnetiP54311.
PhosphoSitePlusiP54311.

Expressioni

Gene expression databases

BgeeiENSRNOG00000016638.
GenevisibleiP54311. RN.

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18 and RASD2. The heterodimer formed by GNB1 and GNG2 interacts with ARHGEF5 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Wasf1Q5BJU72EBI-917779,EBI-7269229

GO - Molecular functioni

  • spectrin binding Source: MGI

Protein-protein interaction databases

BioGridi246567. 5 interactors.
DIPiDIP-37029N.
IntActiP54311. 2 interactors.
MINTiMINT-1795773.
STRINGi10116.ENSRNOP00000044340.

Structurei

Secondary structure

1340
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 24Combined sources22
Helixi30 – 33Combined sources4
Beta strandi35 – 37Combined sources3
Beta strandi47 – 52Combined sources6
Beta strandi58 – 63Combined sources6
Beta strandi67 – 74Combined sources8
Beta strandi77 – 83Combined sources7
Turni84 – 87Combined sources4
Beta strandi88 – 94Combined sources7
Beta strandi96 – 98Combined sources3
Beta strandi100 – 105Combined sources6
Beta strandi109 – 116Combined sources8
Beta strandi121 – 126Combined sources6
Beta strandi129 – 132Combined sources4
Beta strandi135 – 139Combined sources5
Beta strandi146 – 161Combined sources16
Beta strandi166 – 170Combined sources5
Turni171 – 174Combined sources4
Beta strandi175 – 180Combined sources6
Beta strandi187 – 192Combined sources6
Beta strandi196 – 203Combined sources8
Beta strandi208 – 212Combined sources5
Turni213 – 215Combined sources3
Beta strandi217 – 222Combined sources6
Beta strandi229 – 234Combined sources6
Beta strandi238 – 245Combined sources8
Beta strandi250 – 254Combined sources5
Turni255 – 258Combined sources4
Beta strandi259 – 264Combined sources6
Beta strandi273 – 278Combined sources6
Beta strandi280 – 282Combined sources3
Beta strandi284 – 289Combined sources6
Beta strandi294 – 298Combined sources5
Turni299 – 301Combined sources3
Beta strandi303 – 308Combined sources6
Beta strandi315 – 320Combined sources6
Beta strandi327 – 331Combined sources5
Beta strandi334 – 339Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SN6X-ray3.20B2-340[»]
ProteinModelPortaliP54311.
SMRiP54311.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54311.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati53 – 83WD 1Add BLAST31
Repeati95 – 125WD 2Add BLAST31
Repeati141 – 170WD 3Add BLAST30
Repeati182 – 212WD 4Add BLAST31
Repeati224 – 254WD 5Add BLAST31
Repeati268 – 298WD 6Add BLAST31
Repeati310 – 340WD 7Add BLAST31

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0286. Eukaryota.
ENOG410XQUX. LUCA.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP54311.
KOiK04536.
OMAiRIVMRPR.
OrthoDBiEOG091G0A7T.
PhylomeDBiP54311.
TreeFamiTF106149.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54311-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELDQLRQE AEQLKNQIRD ARKACADATL SQITNNIDPV GRIQMRTRRT
60 70 80 90 100
LRGHLAKIYA MHWGTDSRLL VSASQDGKLI IWDSYTTNKV HAIPLRSSWV
110 120 130 140 150
MTCAYAPSGN YVACGGLDNI CSIYNLKTRE GNVRVSRELA GHTGYLSCCR
160 170 180 190 200
FLDDNQIVTS SGDTTCALWD IETGQQTTTF TGHTGDVMSL SLAPDTRLFV
210 220 230 240 250
SGACDASAKL WDVREGMCRQ TFTGHESDIN AICFFPNGNA FATGSDDATC
260 270 280 290 300
RLFDLRADQE LMTYSHDNII CGITSVSFSK SGRLLLAGYD DFNCNVWDAL
310 320 330 340
KADRAGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLKIWN
Length:340
Mass (Da):37,377
Last modified:January 23, 2007 - v4
Checksum:i896CBD32D2686598
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti208A → V in AAD00650 (PubMed:9221795).Curated1
Sequence conflicti277S → P in AAD00650 (PubMed:9221795).Curated1
Sequence conflicti287A → G in AAD00650 (PubMed:9221795).Curated1
Sequence conflicti301K → N in AAD00650 (PubMed:9221795).Curated1
Sequence conflicti307V → D in AAC72249 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34958 mRNA. Translation: AAC72249.1.
U88324 mRNA. Translation: AAD00650.1.
AY552805 mRNA. Translation: AAS59143.1.
BC078809 mRNA. Translation: AAH78809.1.
RefSeqiNP_112249.2. NM_030987.2.
XP_006239579.1. XM_006239517.3.
XP_008762557.1. XM_008764335.2.
XP_017448634.1. XM_017593145.1.
XP_017448635.1. XM_017593146.1.
XP_017448636.1. XM_017593147.1.
XP_017448637.1. XM_017593148.1.
XP_017448638.1. XM_017593149.1.
UniGeneiRn.126047.

Genome annotation databases

EnsembliENSRNOT00000041789; ENSRNOP00000044340; ENSRNOG00000016638.
GeneIDi24400.
KEGGirno:24400.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34958 mRNA. Translation: AAC72249.1.
U88324 mRNA. Translation: AAD00650.1.
AY552805 mRNA. Translation: AAS59143.1.
BC078809 mRNA. Translation: AAH78809.1.
RefSeqiNP_112249.2. NM_030987.2.
XP_006239579.1. XM_006239517.3.
XP_008762557.1. XM_008764335.2.
XP_017448634.1. XM_017593145.1.
XP_017448635.1. XM_017593146.1.
XP_017448636.1. XM_017593147.1.
XP_017448637.1. XM_017593148.1.
XP_017448638.1. XM_017593149.1.
UniGeneiRn.126047.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SN6X-ray3.20B2-340[»]
ProteinModelPortaliP54311.
SMRiP54311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246567. 5 interactors.
DIPiDIP-37029N.
IntActiP54311. 2 interactors.
MINTiMINT-1795773.
STRINGi10116.ENSRNOP00000044340.

PTM databases

iPTMnetiP54311.
PhosphoSitePlusiP54311.

Proteomic databases

PaxDbiP54311.
PRIDEiP54311.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000041789; ENSRNOP00000044340; ENSRNOG00000016638.
GeneIDi24400.
KEGGirno:24400.

Organism-specific databases

CTDi2782.
RGDi2718. Gnb1.

Phylogenomic databases

eggNOGiKOG0286. Eukaryota.
ENOG410XQUX. LUCA.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP54311.
KOiK04536.
OMAiRIVMRPR.
OrthoDBiEOG091G0A7T.
PhylomeDBiP54311.
TreeFamiTF106149.

Enzyme and pathway databases

ReactomeiR-RNO-1296041. Activation of G protein gated Potassium channels.
R-RNO-163359. Glucagon signaling in metabolic regulation.
R-RNO-202040. G-protein activation.
R-RNO-2485179. Activation of the phototransduction cascade.
R-RNO-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-RNO-381753. Olfactory Signaling Pathway.
R-RNO-392170. ADP signalling through P2Y purinoceptor 12.
R-RNO-392451. G beta:gamma signalling through PI3Kgamma.
R-RNO-392851. Prostacyclin signalling through prostacyclin receptor.
R-RNO-400042. Adrenaline,noradrenaline inhibits insulin secretion.
R-RNO-4086398. Ca2+ pathway.
R-RNO-416476. G alpha (q) signalling events.
R-RNO-416482. G alpha (12/13) signalling events.
R-RNO-418217. G beta:gamma signalling through PLC beta.
R-RNO-418555. G alpha (s) signalling events.
R-RNO-418592. ADP signalling through P2Y purinoceptor 1.
R-RNO-418594. G alpha (i) signalling events.
R-RNO-418597. G alpha (z) signalling events.
R-RNO-420092. Glucagon-type ligand receptors.
R-RNO-428930. Thromboxane signalling through TP receptor.
R-RNO-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-RNO-456926. Thrombin signalling through proteinase activated receptors (PARs).
R-RNO-500657. Presynaptic function of Kainate receptors.
R-RNO-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.
R-RNO-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Miscellaneous databases

EvolutionaryTraceiP54311.
PROiP54311.

Gene expression databases

BgeeiENSRNOG00000016638.
GenevisibleiP54311. RN.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGBB1_RAT
AccessioniPrimary (citable) accession number: P54311
Secondary accession number(s): Q6Q8B1, Q9QWG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 143 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.