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P54311 (GBB1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
Alternative name(s):
Transducin beta chain 1
Gene names
Name:Gnb1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Subunit structure

G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18 and RASD2. The heterodimer formed by GNB1 and GNG2 interacts with PTH1R (via C-terminus) By similarity.

Post-translational modification

Phosphorylation at His-266 by NDKB contributes to G protein activation by increasing the high energetic phosphate transfer onto GDP By similarity.

Sequence similarities

Belongs to the WD repeat G protein beta family.

Contains 7 WD repeats.

Ontologies

Keywords
   DomainRepeat
WD repeat
   Molecular functionTransducer
   PTMAcetylation
Phosphoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Inferred from mutant phenotype PubMed 9092554. Source: RGD

cardiac muscle cell apoptotic process

Inferred from direct assay PubMed 19723622. Source: MGI

cell proliferation

Inferred from electronic annotation. Source: Ensembl

cellular response to hypoxia

Inferred from direct assay PubMed 19723622. Source: MGI

phospholipase C-activating G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of cytosolic calcium ion concentration

Inferred from mutant phenotype PubMed 9092554. Source: RGD

retina development in camera-type eye

Inferred from electronic annotation. Source: Ensembl

sensory perception of taste

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcell body

Inferred from direct assay PubMed 9648884. Source: RGD

dendrite

Inferred from direct assay PubMed 9648884. Source: RGD

heterotrimeric G-protein complex

Inferred from direct assay PubMed 11931744. Source: RGD

lysosomal membrane

Inferred from electronic annotation. Source: Ensembl

photoreceptor inner segment

Inferred from direct assay PubMed 11931744. Source: RGD

photoreceptor outer segment membrane

Inferred from direct assay PubMed 11931744. Source: RGD

   Molecular_functionGTPase activity

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction PubMed 16530190. Source: IntAct

signal transducer activity

Inferred from electronic annotation. Source: UniProtKB-KW

spectrin binding

Inferred from direct assay PubMed 23704327. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Wasf1Q5BJU72EBI-917779,EBI-7269229

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 340339Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
PRO_0000127690

Regions

Repeat53 – 8331WD 1
Repeat95 – 12531WD 2
Repeat141 – 17030WD 3
Repeat182 – 21231WD 4
Repeat224 – 25431WD 5
Repeat268 – 29831WD 6
Repeat310 – 34031WD 7

Amino acid modifications

Modified residue21N-acetylserine Ref.6
Modified residue2661Phosphohistidine By similarity

Experimental info

Sequence conflict2081A → V in AAD00650. Ref.2
Sequence conflict2771S → P in AAD00650. Ref.2
Sequence conflict2871A → G in AAD00650. Ref.2
Sequence conflict3011K → N in AAD00650. Ref.2
Sequence conflict3071V → D in AAC72249. Ref.1

Secondary structure

..................................................................... 340
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P54311 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 896CBD32D2686598

FASTA34037,377
        10         20         30         40         50         60 
MSELDQLRQE AEQLKNQIRD ARKACADATL SQITNNIDPV GRIQMRTRRT LRGHLAKIYA 

        70         80         90        100        110        120 
MHWGTDSRLL VSASQDGKLI IWDSYTTNKV HAIPLRSSWV MTCAYAPSGN YVACGGLDNI 

       130        140        150        160        170        180 
CSIYNLKTRE GNVRVSRELA GHTGYLSCCR FLDDNQIVTS SGDTTCALWD IETGQQTTTF 

       190        200        210        220        230        240 
TGHTGDVMSL SLAPDTRLFV SGACDASAKL WDVREGMCRQ TFTGHESDIN AICFFPNGNA 

       250        260        270        280        290        300 
FATGSDDATC RLFDLRADQE LMTYSHDNII CGITSVSFSK SGRLLLAGYD DFNCNVWDAL 

       310        320        330        340 
KADRAGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLKIWN 

« Hide

References

« Hide 'large scale' references
[1]Kuroda S., Tokunaga C., Konishi H., Kikkawa U.
Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"rGbeta1: a psychostimulant-regulated gene essential for establishing cocaine sensitization."
Wang X.B., Funada M., Imai Y., Revay R.S., Ujike H., Vandenbergh D.J., Uhl G.R.
J. Neurosci. 17:5993-6000(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
[3]"Cloning and characterization of the rat G-protein beta 1 subunit."
Puhl H.L. III, Ikeda S.R.
Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Brain.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[5]Lubec G., Diao W., Afjehi-Sadat L., Chen W.-Q., Kang S.U.
Submitted (JUL-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 58-78; 90-96; 138-150; 198-209; 284-301 AND 257-280, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain and Hippocampus.
[6]Lubec G., Chen W.-Q.
Submitted (FEB-2007) to UniProtKB
Cited for: ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U34958 mRNA. Translation: AAC72249.1.
U88324 mRNA. Translation: AAD00650.1.
AY552805 mRNA. Translation: AAS59143.1.
BC078809 mRNA. Translation: AAH78809.1.
RefSeqNP_112249.2. NM_030987.2.
XP_006239579.1. XM_006239517.1.
XP_006239580.1. XM_006239518.1.
XP_006239581.1. XM_006239519.1.
XP_006239582.1. XM_006239520.1.
UniGeneRn.126047.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3SN6X-ray3.20B2-340[»]
ProteinModelPortalP54311.
SMRP54311. Positions 2-340.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid246567. 4 interactions.
DIPDIP-37029N.
IntActP54311. 2 interactions.
MINTMINT-1795773.

PTM databases

PhosphoSiteP54311.

Proteomic databases

PaxDbP54311.
PRIDEP54311.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000041789; ENSRNOP00000044340; ENSRNOG00000016638.
GeneID24400.
KEGGrno:24400.

Organism-specific databases

CTD2782.
RGD2718. Gnb1.

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00740000115196.
HOGENOMHOG000176356.
HOVERGENHBG000188.
InParanoidP54311.
KOK04536.
OMARIVMRPR.
OrthoDBEOG7GN2N5.
PhylomeDBP54311.
TreeFamTF106149.

Gene expression databases

GenevestigatorP54311.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 7 hits.
[Graphical view]
PIRSFPIRSF002394. GN-bd_beta. 1 hit.
PRINTSPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP54311.
NextBio603199.
PROP54311.

Entry information

Entry nameGBB1_RAT
AccessionPrimary (citable) accession number: P54311
Secondary accession number(s): Q6Q8B1, Q9QWG8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 121 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references