Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

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P54311 (GBB1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 111. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Alternative name(s):
Transducin beta chain 1

Gene names

Name:

Gnb1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Subunit structure	G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18 and RASD2. The heterodimer formed by GNB1 and GNG2 interacts with PTH1R (via C-terminus) By similarity.
Post-translational modification	Phosphorylation at His-266 by NDKB contributes to G protein activation by increasing the high energetic phosphate transfer onto GDP By similarity.
Sequence similarities	Belongs to the WD repeat G protein beta family. Contains 7 WD repeats.

Ontologies

Keywords
Domain	Repeat WD repeat
Molecular function	Transducer
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway Inferred from mutant phenotype PubMed 9092554. Source: RGD cardiac muscle cell apoptotic process Inferred from direct assay PubMed 19723622. Source: MGI cell proliferation Inferred from electronic annotation. Source: Compara cellular response to hypoxia Inferred from direct assay PubMed 19723622. Source: MGI elevation of cytosolic calcium ion concentration Inferred from mutant phenotype PubMed 9092554. Source: RGD phospholipase C-activating G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara retina development in camera-type eye Inferred from electronic annotation. Source: Compara sensory perception of taste Inferred from electronic annotation. Source: Compara
Cellular_component	cell body Inferred from direct assay PubMed 9648884. Source: RGD dendrite Inferred from direct assay PubMed 9648884. Source: RGD heterotrimeric G-protein complex Inferred from direct assay PubMed 11931744. Source: RGD photoreceptor inner segment Inferred from direct assay PubMed 11931744. Source: RGD photoreceptor outer segment membrane Inferred from direct assay PubMed 11931744. Source: RGD
Molecular_function	GTPase activity Inferred from electronic annotation. Source: Compara signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed

Chain

2 – 340

339

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

PRO_0000127690

Regions

Repeat

53 – 83

WD 1

Repeat

95 – 125

WD 2

Repeat

141 – 170

WD 3

Repeat

182 – 212

WD 4

Repeat

224 – 254

WD 5

Repeat

268 – 298

WD 6

Repeat

310 – 340

WD 7

Amino acid modifications

Modified residue

N-acetylserine Ref.6

Modified residue

266

Phosphohistidine By similarity

Experimental info

Sequence conflict

208

A → V in AAD00650. Ref.2

Sequence conflict

277

S → P in AAD00650. Ref.2

Sequence conflict

287

A → G in AAD00650. Ref.2

Sequence conflict

301

K → N in AAD00650. Ref.2

Sequence conflict

307

V → D in AAC72249. Ref.1

Secondary structure

340

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P54311 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 896CBD32D2686598
Show »

FASTA

340

37,377

        10         20         30         40         50         60 
MSELDQLRQE AEQLKNQIRD ARKACADATL SQITNNIDPV GRIQMRTRRT LRGHLAKIYA 

        70         80         90        100        110        120 
MHWGTDSRLL VSASQDGKLI IWDSYTTNKV HAIPLRSSWV MTCAYAPSGN YVACGGLDNI 

       130        140        150        160        170        180 
CSIYNLKTRE GNVRVSRELA GHTGYLSCCR FLDDNQIVTS SGDTTCALWD IETGQQTTTF 

       190        200        210        220        230        240 
TGHTGDVMSL SLAPDTRLFV SGACDASAKL WDVREGMCRQ TFTGHESDIN AICFFPNGNA 

       250        260        270        280        290        300 
FATGSDDATC RLFDLRADQE LMTYSHDNII CGITSVSFSK SGRLLLAGYD DFNCNVWDAL 

       310        320        330        340 
KADRAGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLKIWN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Kuroda S., Tokunaga C., Konishi H., Kikkawa U. Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[2]	"rGbeta1: a psychostimulant-regulated gene essential for establishing cocaine sensitization." Wang X.B., Funada M., Imai Y., Revay R.S., Ujike H., Vandenbergh D.J., Uhl G.R. J. Neurosci. 17:5993-6000(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[3]	"Cloning and characterization of the rat G-protein beta 1 subunit." Puhl H.L. III, Ikeda S.R. Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[5]	Lubec G., Diao W., Afjehi-Sadat L., Chen W.-Q., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 58-78; 90-96; 138-150; 198-209; 284-301 AND 257-280, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain and Hippocampus.
[6]	Lubec G., Chen W.-Q. Submitted (FEB-2007) to UniProtKB Cited for: ACETYLATION AT SER-2, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U34958 mRNA. Translation: AAC72249.1.
U88324 mRNA. Translation: AAD00650.1.
AY552805 mRNA. Translation: AAS59143.1.
BC078809 mRNA. Translation: AAH78809.1.

IPI

IPI00212655.

RefSeq

NP_112249.2. NM_030987.2.

UniGene

Rn.126047.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3SN6	X-ray	3.20	B	2-340	[»]

ProteinModelPortal

P54311.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-37029N.

IntAct

P54311. 1 interaction.

MINT

MINT-1795773.

PTM databases

PhosphoSite

P54311.

Proteomic databases

PaxDb

P54311.

PRIDE

P54311.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000041789; ENSRNOP00000044340; ENSRNOG00000016638.

GeneID

24400.

KEGG

rno:24400.

Organism-specific databases

CTD

2782.

RGD

2718. Gnb1.

Phylogenomic databases

eggNOG

COG2319.

GeneTree

ENSGT00550000074331.

HOGENOM

HOG000176356.

HOVERGEN

HBG000188.

InParanoid

P54311.

K04536.

OMA

CMLWDIE.

OrthoDB

EOG4HX51D.

Gene expression databases

ArrayExpress

P54311.

Genevestigator

P54311.

GermOnline

ENSRNOG00000016638. Rattus norvegicus.

Family and domain databases

Gene3D

2.130.10.10. 1 hit.

InterPro

IPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]

Pfam

PF00400. WD40. 7 hits.
[Graphical view]

PIRSF

PIRSF002394. GN-bd_beta. 1 hit.

PRINTS

PR00319. GPROTEINB.
PR00320. GPROTEINBRPT.

SMART

SM00320. WD40. 7 hits.
[Graphical view]

SUPFAM

SSF50978. WD40_like. 1 hit.

PROSITE

PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P54311.

NextBio

603199.

Entry information

Entry name

GBB1_RAT

Accession

Primary (citable) accession number: P54311
Secondary accession number(s): Q6Q8B1, Q9QWG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 111 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents