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P54311

- GBB1_RAT

UniProt

P54311 - GBB1_RAT

Protein

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Gene

Gnb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

    GO - Molecular functioni

    1. GTPase activity Source: Ensembl
    2. protein binding Source: IntAct
    3. signal transducer activity Source: UniProtKB-KW
    4. spectrin binding Source: MGI

    GO - Biological processi

    1. cardiac muscle cell apoptotic process Source: MGI
    2. cell proliferation Source: Ensembl
    3. cellular response to hypoxia Source: MGI
    4. G-protein coupled receptor signaling pathway Source: RGD
    5. phospholipase C-activating G-protein coupled receptor signaling pathway Source: Ensembl
    6. positive regulation of cytosolic calcium ion concentration Source: RGD
    7. retina development in camera-type eye Source: Ensembl
    8. sensory perception of taste Source: Ensembl

    Keywords - Molecular functioni

    Transducer

    Enzyme and pathway databases

    ReactomeiREACT_194690. Ca2+ pathway.
    REACT_196412. Presynaptic function of Kainate receptors.
    REACT_202899. Activation of the phototransduction cascade.
    REACT_208646. G beta:gamma signalling through PLC beta.
    REACT_227662. Inactivation, recovery and regulation of the phototransduction cascade.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
    Alternative name(s):
    Transducin beta chain 1
    Gene namesi
    Name:Gnb1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 5

    Organism-specific databases

    RGDi2718. Gnb1.

    Subcellular locationi

    GO - Cellular componenti

    1. cell body Source: RGD
    2. dendrite Source: RGD
    3. heterotrimeric G-protein complex Source: RGD
    4. lysosomal membrane Source: Ensembl
    5. photoreceptor inner segment Source: RGD
    6. photoreceptor outer segment membrane Source: RGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 340339Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1PRO_0000127690Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei266 – 2661PhosphohistidineBy similarity

    Post-translational modificationi

    Phosphorylation at His-266 by NDKB contributes to G protein activation by increasing the high energetic phosphate transfer onto GDP.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP54311.
    PRIDEiP54311.

    PTM databases

    PhosphoSiteiP54311.

    Expressioni

    Gene expression databases

    GenevestigatoriP54311.

    Interactioni

    Subunit structurei

    G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18 and RASD2. The heterodimer formed by GNB1 and GNG2 interacts with PTH1R (via C-terminus) By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Wasf1Q5BJU72EBI-917779,EBI-7269229

    Protein-protein interaction databases

    BioGridi246567. 4 interactions.
    DIPiDIP-37029N.
    IntActiP54311. 2 interactions.
    MINTiMINT-1795773.

    Structurei

    Secondary structure

    1
    340
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 2422
    Helixi30 – 334
    Beta strandi35 – 373
    Beta strandi47 – 526
    Beta strandi58 – 636
    Beta strandi67 – 748
    Beta strandi77 – 837
    Turni84 – 874
    Beta strandi88 – 947
    Beta strandi96 – 983
    Beta strandi100 – 1056
    Beta strandi109 – 1168
    Beta strandi121 – 1266
    Beta strandi129 – 1324
    Beta strandi135 – 1395
    Beta strandi146 – 16116
    Beta strandi166 – 1705
    Turni171 – 1744
    Beta strandi175 – 1806
    Beta strandi187 – 1926
    Beta strandi196 – 2038
    Beta strandi208 – 2125
    Turni213 – 2153
    Beta strandi217 – 2226
    Beta strandi229 – 2346
    Beta strandi238 – 2458
    Beta strandi250 – 2545
    Turni255 – 2584
    Beta strandi259 – 2646
    Beta strandi273 – 2786
    Beta strandi280 – 2823
    Beta strandi284 – 2896
    Beta strandi294 – 2985
    Turni299 – 3013
    Beta strandi303 – 3086
    Beta strandi315 – 3206
    Beta strandi327 – 3315
    Beta strandi334 – 3396

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3SN6X-ray3.20B2-340[»]
    ProteinModelPortaliP54311.
    SMRiP54311. Positions 2-340.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP54311.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati53 – 8331WD 1Add
    BLAST
    Repeati95 – 12531WD 2Add
    BLAST
    Repeati141 – 17030WD 3Add
    BLAST
    Repeati182 – 21231WD 4Add
    BLAST
    Repeati224 – 25431WD 5Add
    BLAST
    Repeati268 – 29831WD 6Add
    BLAST
    Repeati310 – 34031WD 7Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat G protein beta family.Curated
    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00740000115196.
    HOGENOMiHOG000176356.
    HOVERGENiHBG000188.
    InParanoidiP54311.
    KOiK04536.
    OMAiRIVMRPR.
    OrthoDBiEOG7GN2N5.
    PhylomeDBiP54311.
    TreeFamiTF106149.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR020472. G-protein_beta_WD-40_rep.
    IPR001632. Gprotein_B.
    IPR016346. Guanine_nucleotide-bd_bsu.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 7 hits.
    [Graphical view]
    PIRSFiPIRSF002394. GN-bd_beta. 1 hit.
    PRINTSiPR00319. GPROTEINB.
    PR00320. GPROTEINBRPT.
    SMARTiSM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 3 hits.
    PS50082. WD_REPEATS_2. 6 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P54311-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSELDQLRQE AEQLKNQIRD ARKACADATL SQITNNIDPV GRIQMRTRRT    50
    LRGHLAKIYA MHWGTDSRLL VSASQDGKLI IWDSYTTNKV HAIPLRSSWV 100
    MTCAYAPSGN YVACGGLDNI CSIYNLKTRE GNVRVSRELA GHTGYLSCCR 150
    FLDDNQIVTS SGDTTCALWD IETGQQTTTF TGHTGDVMSL SLAPDTRLFV 200
    SGACDASAKL WDVREGMCRQ TFTGHESDIN AICFFPNGNA FATGSDDATC 250
    RLFDLRADQE LMTYSHDNII CGITSVSFSK SGRLLLAGYD DFNCNVWDAL 300
    KADRAGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLKIWN 340
    Length:340
    Mass (Da):37,377
    Last modified:January 23, 2007 - v4
    Checksum:i896CBD32D2686598
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti208 – 2081A → V in AAD00650. (PubMed:9221795)Curated
    Sequence conflicti277 – 2771S → P in AAD00650. (PubMed:9221795)Curated
    Sequence conflicti287 – 2871A → G in AAD00650. (PubMed:9221795)Curated
    Sequence conflicti301 – 3011K → N in AAD00650. (PubMed:9221795)Curated
    Sequence conflicti307 – 3071V → D in AAC72249. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U34958 mRNA. Translation: AAC72249.1.
    U88324 mRNA. Translation: AAD00650.1.
    AY552805 mRNA. Translation: AAS59143.1.
    BC078809 mRNA. Translation: AAH78809.1.
    RefSeqiNP_112249.2. NM_030987.2.
    XP_006239579.1. XM_006239517.1.
    XP_006239580.1. XM_006239518.1.
    XP_006239581.1. XM_006239519.1.
    XP_006239582.1. XM_006239520.1.
    UniGeneiRn.126047.

    Genome annotation databases

    EnsembliENSRNOT00000041789; ENSRNOP00000044340; ENSRNOG00000016638.
    GeneIDi24400.
    KEGGirno:24400.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U34958 mRNA. Translation: AAC72249.1 .
    U88324 mRNA. Translation: AAD00650.1 .
    AY552805 mRNA. Translation: AAS59143.1 .
    BC078809 mRNA. Translation: AAH78809.1 .
    RefSeqi NP_112249.2. NM_030987.2.
    XP_006239579.1. XM_006239517.1.
    XP_006239580.1. XM_006239518.1.
    XP_006239581.1. XM_006239519.1.
    XP_006239582.1. XM_006239520.1.
    UniGenei Rn.126047.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3SN6 X-ray 3.20 B 2-340 [» ]
    ProteinModelPortali P54311.
    SMRi P54311. Positions 2-340.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246567. 4 interactions.
    DIPi DIP-37029N.
    IntActi P54311. 2 interactions.
    MINTi MINT-1795773.

    PTM databases

    PhosphoSitei P54311.

    Proteomic databases

    PaxDbi P54311.
    PRIDEi P54311.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000041789 ; ENSRNOP00000044340 ; ENSRNOG00000016638 .
    GeneIDi 24400.
    KEGGi rno:24400.

    Organism-specific databases

    CTDi 2782.
    RGDi 2718. Gnb1.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00740000115196.
    HOGENOMi HOG000176356.
    HOVERGENi HBG000188.
    InParanoidi P54311.
    KOi K04536.
    OMAi RIVMRPR.
    OrthoDBi EOG7GN2N5.
    PhylomeDBi P54311.
    TreeFami TF106149.

    Enzyme and pathway databases

    Reactomei REACT_194690. Ca2+ pathway.
    REACT_196412. Presynaptic function of Kainate receptors.
    REACT_202899. Activation of the phototransduction cascade.
    REACT_208646. G beta:gamma signalling through PLC beta.
    REACT_227662. Inactivation, recovery and regulation of the phototransduction cascade.

    Miscellaneous databases

    EvolutionaryTracei P54311.
    NextBioi 603199.
    PROi P54311.

    Gene expression databases

    Genevestigatori P54311.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR020472. G-protein_beta_WD-40_rep.
    IPR001632. Gprotein_B.
    IPR016346. Guanine_nucleotide-bd_bsu.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 7 hits.
    [Graphical view ]
    PIRSFi PIRSF002394. GN-bd_beta. 1 hit.
    PRINTSi PR00319. GPROTEINB.
    PR00320. GPROTEINBRPT.
    SMARTi SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 3 hits.
    PS50082. WD_REPEATS_2. 6 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Kuroda S., Tokunaga C., Konishi H., Kikkawa U.
      Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Brain.
    2. "rGbeta1: a psychostimulant-regulated gene essential for establishing cocaine sensitization."
      Wang X.B., Funada M., Imai Y., Revay R.S., Ujike H., Vandenbergh D.J., Uhl G.R.
      J. Neurosci. 17:5993-6000(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
    3. "Cloning and characterization of the rat G-protein beta 1 subunit."
      Puhl H.L. III, Ikeda S.R.
      Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Brain.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.
    5. Lubec G., Diao W., Afjehi-Sadat L., Chen W.-Q., Kang S.U.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 58-78; 90-96; 138-150; 198-209; 284-301 AND 257-280, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain and Hippocampus.
    6. Lubec G., Chen W.-Q.
      Submitted (FEB-2007) to UniProtKB
      Cited for: ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiGBB1_RAT
    AccessioniPrimary (citable) accession number: P54311
    Secondary accession number(s): Q6Q8B1, Q9QWG8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 123 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3