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P54311

- GBB1_RAT

UniProt

P54311 - GBB1_RAT

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Protein

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Gene

Gnb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

GO - Molecular functioni

  1. GTPase activity Source: Ensembl
  2. signal transducer activity Source: UniProtKB-KW
  3. spectrin binding Source: MGI

GO - Biological processi

  1. cardiac muscle cell apoptotic process Source: MGI
  2. cell proliferation Source: Ensembl
  3. cellular response to hypoxia Source: MGI
  4. G-protein coupled receptor signaling pathway Source: RGD
  5. phospholipase C-activating G-protein coupled receptor signaling pathway Source: Ensembl
  6. positive regulation of cytosolic calcium ion concentration Source: RGD
  7. retina development in camera-type eye Source: Ensembl
  8. sensory perception of taste Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Enzyme and pathway databases

ReactomeiREACT_194690. Ca2+ pathway.
REACT_196412. Presynaptic function of Kainate receptors.
REACT_202899. Activation of the phototransduction cascade.
REACT_208646. G beta:gamma signalling through PLC beta.
REACT_227662. Inactivation, recovery and regulation of the phototransduction cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
Alternative name(s):
Transducin beta chain 1
Gene namesi
Name:Gnb1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 5

Organism-specific databases

RGDi2718. Gnb1.

Subcellular locationi

GO - Cellular componenti

  1. cell body Source: RGD
  2. dendrite Source: RGD
  3. extracellular vesicular exosome Source: Ensembl
  4. heterotrimeric G-protein complex Source: RGD
  5. lysosomal membrane Source: Ensembl
  6. photoreceptor inner segment Source: RGD
  7. photoreceptor outer segment membrane Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 340339Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1PRO_0000127690Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei266 – 2661PhosphohistidineBy similarity

Post-translational modificationi

Phosphorylation at His-266 by NDKB contributes to G protein activation by increasing the high energetic phosphate transfer onto GDP.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP54311.
PRIDEiP54311.

PTM databases

PhosphoSiteiP54311.

Expressioni

Gene expression databases

ExpressionAtlasiP54311. baseline.
GenevestigatoriP54311.

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18 and RASD2. The heterodimer formed by GNB1 and GNG2 interacts with PTH1R (via C-terminus) (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Wasf1Q5BJU72EBI-917779,EBI-7269229

Protein-protein interaction databases

BioGridi246567. 5 interactions.
DIPiDIP-37029N.
IntActiP54311. 2 interactions.
MINTiMINT-1795773.

Structurei

Secondary structure

1
340
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 2422Combined sources
Helixi30 – 334Combined sources
Beta strandi35 – 373Combined sources
Beta strandi47 – 526Combined sources
Beta strandi58 – 636Combined sources
Beta strandi67 – 748Combined sources
Beta strandi77 – 837Combined sources
Turni84 – 874Combined sources
Beta strandi88 – 947Combined sources
Beta strandi96 – 983Combined sources
Beta strandi100 – 1056Combined sources
Beta strandi109 – 1168Combined sources
Beta strandi121 – 1266Combined sources
Beta strandi129 – 1324Combined sources
Beta strandi135 – 1395Combined sources
Beta strandi146 – 16116Combined sources
Beta strandi166 – 1705Combined sources
Turni171 – 1744Combined sources
Beta strandi175 – 1806Combined sources
Beta strandi187 – 1926Combined sources
Beta strandi196 – 2038Combined sources
Beta strandi208 – 2125Combined sources
Turni213 – 2153Combined sources
Beta strandi217 – 2226Combined sources
Beta strandi229 – 2346Combined sources
Beta strandi238 – 2458Combined sources
Beta strandi250 – 2545Combined sources
Turni255 – 2584Combined sources
Beta strandi259 – 2646Combined sources
Beta strandi273 – 2786Combined sources
Beta strandi280 – 2823Combined sources
Beta strandi284 – 2896Combined sources
Beta strandi294 – 2985Combined sources
Turni299 – 3013Combined sources
Beta strandi303 – 3086Combined sources
Beta strandi315 – 3206Combined sources
Beta strandi327 – 3315Combined sources
Beta strandi334 – 3396Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3SN6X-ray3.20B2-340[»]
ProteinModelPortaliP54311.
SMRiP54311. Positions 2-340.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54311.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati53 – 8331WD 1Add
BLAST
Repeati95 – 12531WD 2Add
BLAST
Repeati141 – 17030WD 3Add
BLAST
Repeati182 – 21231WD 4Add
BLAST
Repeati224 – 25431WD 5Add
BLAST
Repeati268 – 29831WD 6Add
BLAST
Repeati310 – 34031WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP54311.
KOiK04536.
OMAiRIVMRPR.
OrthoDBiEOG7GN2N5.
PhylomeDBiP54311.
TreeFamiTF106149.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 7 hits.
[Graphical view]
PIRSFiPIRSF002394. GN-bd_beta. 1 hit.
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54311-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSELDQLRQE AEQLKNQIRD ARKACADATL SQITNNIDPV GRIQMRTRRT
60 70 80 90 100
LRGHLAKIYA MHWGTDSRLL VSASQDGKLI IWDSYTTNKV HAIPLRSSWV
110 120 130 140 150
MTCAYAPSGN YVACGGLDNI CSIYNLKTRE GNVRVSRELA GHTGYLSCCR
160 170 180 190 200
FLDDNQIVTS SGDTTCALWD IETGQQTTTF TGHTGDVMSL SLAPDTRLFV
210 220 230 240 250
SGACDASAKL WDVREGMCRQ TFTGHESDIN AICFFPNGNA FATGSDDATC
260 270 280 290 300
RLFDLRADQE LMTYSHDNII CGITSVSFSK SGRLLLAGYD DFNCNVWDAL
310 320 330 340
KADRAGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLKIWN
Length:340
Mass (Da):37,377
Last modified:January 23, 2007 - v4
Checksum:i896CBD32D2686598
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti208 – 2081A → V in AAD00650. (PubMed:9221795)Curated
Sequence conflicti277 – 2771S → P in AAD00650. (PubMed:9221795)Curated
Sequence conflicti287 – 2871A → G in AAD00650. (PubMed:9221795)Curated
Sequence conflicti301 – 3011K → N in AAD00650. (PubMed:9221795)Curated
Sequence conflicti307 – 3071V → D in AAC72249. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U34958 mRNA. Translation: AAC72249.1.
U88324 mRNA. Translation: AAD00650.1.
AY552805 mRNA. Translation: AAS59143.1.
BC078809 mRNA. Translation: AAH78809.1.
RefSeqiNP_112249.2. NM_030987.2.
XP_006239579.1. XM_006239517.2.
XP_006239580.1. XM_006239518.2.
XP_006239581.1. XM_006239519.2.
XP_006239582.1. XM_006239520.2.
UniGeneiRn.126047.

Genome annotation databases

EnsembliENSRNOT00000041789; ENSRNOP00000044340; ENSRNOG00000016638.
GeneIDi24400.
KEGGirno:24400.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U34958 mRNA. Translation: AAC72249.1 .
U88324 mRNA. Translation: AAD00650.1 .
AY552805 mRNA. Translation: AAS59143.1 .
BC078809 mRNA. Translation: AAH78809.1 .
RefSeqi NP_112249.2. NM_030987.2.
XP_006239579.1. XM_006239517.2.
XP_006239580.1. XM_006239518.2.
XP_006239581.1. XM_006239519.2.
XP_006239582.1. XM_006239520.2.
UniGenei Rn.126047.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3SN6 X-ray 3.20 B 2-340 [» ]
ProteinModelPortali P54311.
SMRi P54311. Positions 2-340.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 246567. 5 interactions.
DIPi DIP-37029N.
IntActi P54311. 2 interactions.
MINTi MINT-1795773.

PTM databases

PhosphoSitei P54311.

Proteomic databases

PaxDbi P54311.
PRIDEi P54311.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000041789 ; ENSRNOP00000044340 ; ENSRNOG00000016638 .
GeneIDi 24400.
KEGGi rno:24400.

Organism-specific databases

CTDi 2782.
RGDi 2718. Gnb1.

Phylogenomic databases

eggNOGi COG2319.
GeneTreei ENSGT00760000119239.
HOGENOMi HOG000176356.
HOVERGENi HBG000188.
InParanoidi P54311.
KOi K04536.
OMAi RIVMRPR.
OrthoDBi EOG7GN2N5.
PhylomeDBi P54311.
TreeFami TF106149.

Enzyme and pathway databases

Reactomei REACT_194690. Ca2+ pathway.
REACT_196412. Presynaptic function of Kainate receptors.
REACT_202899. Activation of the phototransduction cascade.
REACT_208646. G beta:gamma signalling through PLC beta.
REACT_227662. Inactivation, recovery and regulation of the phototransduction cascade.

Miscellaneous databases

EvolutionaryTracei P54311.
NextBioi 603199.
PROi P54311.

Gene expression databases

ExpressionAtlasi P54311. baseline.
Genevestigatori P54311.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF00400. WD40. 7 hits.
[Graphical view ]
PIRSFi PIRSF002394. GN-bd_beta. 1 hit.
PRINTSi PR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTi SM00320. WD40. 7 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Kuroda S., Tokunaga C., Konishi H., Kikkawa U.
    Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "rGbeta1: a psychostimulant-regulated gene essential for establishing cocaine sensitization."
    Wang X.B., Funada M., Imai Y., Revay R.S., Ujike H., Vandenbergh D.J., Uhl G.R.
    J. Neurosci. 17:5993-6000(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  3. "Cloning and characterization of the rat G-protein beta 1 subunit."
    Puhl H.L. III, Ikeda S.R.
    Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  5. Lubec G., Diao W., Afjehi-Sadat L., Chen W.-Q., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 58-78; 90-96; 138-150; 198-209; 284-301 AND 257-280, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain and Hippocampus.
  6. Lubec G., Chen W.-Q.
    Submitted (FEB-2007) to UniProtKB
    Cited for: ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiGBB1_RAT
AccessioniPrimary (citable) accession number: P54311
Secondary accession number(s): Q6Q8B1, Q9QWG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 124 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3