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Protein

Terminase small subunit

Gene

1

Organism
Bacillus phage SPP1 (Bacteriophage SPP1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The terminase small subunit specifically recognizes the non-adjacent pacL and pacR packaging subsites and regulates the ATPase activity of the terminase large subunit. The terminase lies at a unique vertex of the procapsid and is composed of two subunits, a small terminase subunit involved in viral DNA recognition (packaging sequence), and a large terminase subunit possessing endonucleolytic and ATPase activities. Both terminase subunits heterooligomerize and are docked on the portal protein to form the packaging machine. The terminase large subunit exhibits endonuclease activity and cleaves the viral genome concatemer once the capsid is full (headful packaging). Once the capsid is packaged with the DNA, the terminase complex is substituted by neck proteins.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Viral genome packaging, Virus exit from host cell

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Terminase small subunit
Alternative name(s):
G1P
Terminase, small subunit gp1
Gene namesi
Name:1
OrganismiBacillus phage SPP1 (Bacteriophage SPP1)
Taxonomic identifieri10724 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdalikevirusunclassified Lambda-like viruses
Virus hostiBacillus subtilis [TaxID: 1423]
Proteomesi
  • UP000002559 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved; by host1 Publication
Chaini2 – 147146Terminase small subunitPRO_0000003378Add
BLAST

Interactioni

Subunit structurei

Homodecamer. Interacts with the terminase large subunit gp2; the active complex is probably composed of a one monomer of gp2 and two or more decamers of gp1.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP54307.
SMRiP54307. Positions 7-61.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SPP1-like small terminase family.Curated

Phylogenomic databases

KOiK07474.

Family and domain databases

InterProiIPR005335. Terminase_ssu.
[Graphical view]
PfamiPF03592. Terminase_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform G1P (identifier: P54307-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGEVKGKWTP KLERFVDEYF INGMNATKAA IAAGYSKKSA STIAAENMQK
60 70 80 90 100
PHVRARIEER LAQMDKKRIM QAEEVLEHLT RIALGQEKEQ VLMGIGKGAE
110 120 130 140
TKTHVEVSAK DRIKALELLG KAHAVFTDKQ KVETNQVIIV DDSGDAE
Length:147
Mass (Da):16,334
Last modified:September 16, 2015 - v4
Checksum:i6C0D4932CDDAE520
GO
Isoform G1P* (identifier: P54307-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-63: Missing.

Show »
Length:84
Mass (Da):9,312
Checksum:i9B3337453E5E32FC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6363Missing in isoform G1P*. CuratedVSP_018686Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56064 Genomic DNA. Translation: CAA39536.1. Sequence problems.
X97918 Genomic DNA. Translation: CAA66571.1. Sequence problems.
AM268239 Genomic DNA. Translation: CAK29440.1.
PIRiS24450.
RefSeqiNP_690652.1. NC_004166.2.

Genome annotation databases

GeneIDi955251.
KEGGivg:955251.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56064 Genomic DNA. Translation: CAA39536.1. Sequence problems.
X97918 Genomic DNA. Translation: CAA66571.1. Sequence problems.
AM268239 Genomic DNA. Translation: CAK29440.1.
PIRiS24450.
RefSeqiNP_690652.1. NC_004166.2.

3D structure databases

ProteinModelPortaliP54307.
SMRiP54307. Positions 7-61.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi955251.
KEGGivg:955251.

Phylogenomic databases

KOiK07474.

Family and domain databases

InterProiIPR005335. Terminase_ssu.
[Graphical view]
PfamiPF03592. Terminase_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular analysis of the Bacillus subtilis bacteriophage SPP1 region encompassing genes 1 to 6. The products of gene 1 and gene 2 are required for pac cleavage."
    Chai S., Bravo A., Lueder G., Nedlin A., Trautner T.A., Alonso J.C.
    J. Mol. Biol. 224:87-102(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of the Bacillus subtilis bacteriophages SPP1 and SF6 gene 1 product: a protein involved in the initiation of headful packaging."
    Chai S., Kruft V., Alonso J.C.
    Virology 202:930-939(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-26 AND 64-91.
  3. "The complete nucleotide sequence and functional organization of Bacillus subtilis bacteriophage SPP1."
    Alonso J.C., Luder G., Stiege A.C., Chai S., Weise F., Trautner T.A.
    Gene 204:201-212(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The DNA-binding domain of bacteriophage SF6 small terminase subunit."
    Benini S., Chechik M., Ortiz-Lombardia M., Polier S., Shevtsov M.B., De Luchi D., Alonso J.C., Antson A.A.
    Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Bacillus subtilis bacteriophage SPP1 DNA packaging motor requires terminase and portal proteins."
    Camacho A.G., Gual A., Lurz R., Tavares P., Alonso J.C.
    J. Biol. Chem. 278:23251-23259(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Headful DNA packaging: bacteriophage SPP1 as a model system."
    Oliveira L., Tavares P., Alonso J.C.
    Virus Res. 173:247-259(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiTERS_BPSPP
AccessioniPrimary (citable) accession number: P54307
Secondary accession number(s): Q1EJR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 16, 2015
Last modified: February 17, 2016
This is version 74 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.