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Protein

Autoinducer 2 sensor kinase/phosphatase LuxQ

Gene

luxQ

Organism
Vibrio harveyi (Beneckea harveyi)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

At low cell density, in absence of AI-2 (autoinducer 2), LuxQ has a kinase activity and autophosphorylates on a histidine residue. The phosphoryl group is then transferred to an aspartate residue in the response regulator domain. The phosphoryl group is transferred to LuxU, and ultimately to LuxO. At high cell density, in the presence of AI-2, the kinase activity is inactivated, and the response regulator domain has a phosphatase activity.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

  • signal transduction by protein phosphorylation Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Protein phosphatase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.13.3. 6632.

Names & Taxonomyi

Protein namesi
Recommended name:
Autoinducer 2 sensor kinase/phosphatase LuxQ (EC:2.7.13.3, EC:3.1.3.-)
Gene namesi
Name:luxQ
OrganismiVibrio harveyi (Beneckea harveyi)
Taxonomic identifieri669 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei15 – 35HelicalSequence analysisAdd BLAST21
Transmembranei280 – 300HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000747831 – 859Autoinducer 2 sensor kinase/phosphatase LuxQAdd BLAST859

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei492Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1
Modified residuei7854-aspartylphosphatePROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Binds the complex formed by AI-2 and LuxP.

Binary interactionsi

WithEntry#Exp.IntActNotes
luxPP543005EBI-1101486,EBI-1101482

Protein-protein interaction databases

IntActiP54302. 1 interactor.
STRINGi338187.VIBHAR_05352.

Structurei

Secondary structure

1859
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi53 – 78Combined sources26
Helixi82 – 90Combined sources9
Helixi93 – 106Combined sources14
Helixi108 – 110Combined sources3
Beta strandi113 – 118Combined sources6
Beta strandi120 – 126Combined sources7
Helixi129 – 133Combined sources5
Helixi137 – 146Combined sources10
Beta strandi149 – 152Combined sources4
Beta strandi154 – 160Combined sources7
Beta strandi163 – 175Combined sources13
Turni177 – 179Combined sources3
Beta strandi182 – 191Combined sources10
Helixi196 – 205Combined sources10
Beta strandi209 – 215Combined sources7
Beta strandi218 – 222Combined sources5
Helixi232 – 236Combined sources5
Beta strandi249 – 257Combined sources9
Beta strandi260 – 270Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZHHX-ray1.94B39-278[»]
2HJ9X-ray2.34C/D53-271[»]
2HJEX-ray1.70A53-271[»]
ProteinModelPortaliP54302.
SMRiP54302.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54302.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini489 – 711Histidine kinasePROSITE-ProRule annotationAdd BLAST223
Domaini736 – 851Response regulatoryPROSITE-ProRule annotationAdd BLAST116

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR015387. LuxQ-periplasm.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF09308. LuxQ-periplasm. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
ProDomiPD142495. LuxQ-periplasm. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54302-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTTRSNIKK RRSLATLITK IIILVLAPII LGIFIQSYYF SKQIIWQEVD
60 70 80 90 100
RTKQQTSALI HNIFDSHFAA IQIHHDSNSK SEVIRDFYTD RDTDVLNFFF
110 120 130 140 150
LSIDQSDPSH TPEFRFLTDH KGIIWDDGNA HFYGVNDLIL DSLANRVSFS
160 170 180 190 200
NNWYYINVMT SIGSRHMLVR RVPILDPSTG EVLGFSFNAV VLDNNFALME
210 220 230 240 250
KLKSESNVDN VVLVANSVPL ANSLIGDEPY NVADVLQRKS SDKRLDKLLV
260 270 280 290 300
IETPIVVNAV TTELCLLTVQ DNQSVVTLQI QHILAMLASI IGMIMIALMS
310 320 330 340 350
REWIESKVSA QLESLMSYTR SAREEKGFER FGGSDIEEFD HIGSTLESTF
360 370 380 390 400
EELEAQKKSF RDLFNFALSP IMVWSEESVL IQMNPAARKE LVIEDDHEIM
410 420 430 440 450
HPVFQGFKEK LTPHLKMAAQ GATLTGVNVP IGNKIYRWNL SPIRVDGDIS
460 470 480 490 500
GIIVQGQDIT TLIEAEKQSN IARREAEKSA QARADFLAKM SHEIRTPING
510 520 530 540 550
ILGVAQLLKD SVDTQEQKNQ IDVLCHSGEH LLAVLNDILD FSKIEQGKFN
560 570 580 590 600
IQKHPFSFTD TMRTLENIYR PICTNKGVEL VIENELDPNV EIFTDQVRLN
610 620 630 640 650
QILFNLVSNA VKFTPIGSIR LHAELEQFYG AENSVLVVEL TDTGIGIESD
660 670 680 690 700
KLDQMFEPFV QEESTTTREY GGSGLGLTIV KNLVDMLEGD VQVRSSKGGG
710 720 730 740 750
TTFVITLPVK DRERVLRPLE VSQRIKPEAL FDESLKVLLV EDNHTNAFIL
760 770 780 790 800
QAFCKKYKMQ VDWAKDGLDA MELLSDTTYD LILMDNQLPH LGGIETTHEI
810 820 830 840 850
RQNLRLGTPI YACTADTAKE TSDAFMAAGA NYVMLKPIKE NALHEAFVDF

KQRFLVERT
Length:859
Mass (Da):96,915
Last modified:October 1, 1996 - v1
Checksum:iAB2171F454C81060
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07069 Genomic DNA. Translation: AAA20838.1.
PIRiS49046.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07069 Genomic DNA. Translation: AAA20838.1.
PIRiS49046.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZHHX-ray1.94B39-278[»]
2HJ9X-ray2.34C/D53-271[»]
2HJEX-ray1.70A53-271[»]
ProteinModelPortaliP54302.
SMRiP54302.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP54302. 1 interactor.
STRINGi338187.VIBHAR_05352.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.

Enzyme and pathway databases

BRENDAi2.7.13.3. 6632.

Miscellaneous databases

EvolutionaryTraceiP54302.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR015387. LuxQ-periplasm.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF09308. LuxQ-periplasm. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
ProDomiPD142495. LuxQ-periplasm. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLUXQ_VIBHA
AccessioniPrimary (citable) accession number: P54302
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.