ID   MYOM2_HUMAN             Reviewed;        1465 AA.
AC   P54296; Q7Z3Y2;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   23-FEB-2022, sequence version 3.
DT   27-MAR-2024, entry version 185.
DE   RecName: Full=Myomesin-2;
DE   AltName: Full=165 kDa connectin-associated protein;
DE   AltName: Full=165 kDa titin-associated protein;
DE   AltName: Full=M-protein;
DE   AltName: Full=Myomesin family member 2;
GN   Name=MYOM2;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND VARIANTS LEU-321 AND ALA-1168.
RC   TISSUE=Skeletal muscle;
RX   PubMed=7505783; DOI=10.1242/jcs.106.1.319;
RA   Vinkemeier U., Obermann W., Weber K., Fuerst D.O.;
RT   "The globular head domain of titin extends into the center of the
RT   sarcomeric M band. cDNA cloning, epitope mapping and immunoelectron
RT   microscopy of two titin-associated proteins.";
RL   J. Cell Sci. 106:319-330(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16421571; DOI=10.1038/nature04406;
RA   Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M.,
RA   Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L.,
RA   Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S.,
RA   Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A.,
RA   Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III,
RA   Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K.,
RA   Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA   Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA   Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B.,
RA   O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K.,
RA   Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L.,
RA   Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G.,
RA   Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W.,
RA   Platzer M., Shimizu N., Lander E.S.;
RT   "DNA sequence and analysis of human chromosome 8.";
RL   Nature 439:331-335(2006).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS MET-776 AND ALA-1168.
RC   TISSUE=PNS;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Major component of the vertebrate myofibrillar M band. Binds
CC       myosin, titin, and light meromyosin. This binding is dose dependent.
CC   -!- SUBUNIT: Interacts with TTN/titin. {ECO:0000250}.
CC   -!- INTERACTION:
CC       P54296; O75923: DYSF; NbExp=3; IntAct=EBI-5357134, EBI-2799016;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, myofibril, sarcomere, M line
CC       {ECO:0000250}.
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DR   EMBL; X69089; CAA48832.1; -; mRNA.
DR   EMBL; AC245164; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC052969; AAH52969.1; -; mRNA.
DR   CCDS; CCDS5957.1; -.
DR   PIR; S43529; S43529.
DR   RefSeq; NP_003961.3; NM_003970.3.
DR   AlphaFoldDB; P54296; -.
DR   SMR; P54296; -.
DR   BioGRID; 114612; 27.
DR   IntAct; P54296; 19.
DR   MINT; P54296; -.
DR   STRING; 9606.ENSP00000262113; -.
DR   GlyGen; P54296; 2 sites, 1 O-linked glycan (2 sites).
DR   iPTMnet; P54296; -.
DR   PhosphoSitePlus; P54296; -.
DR   BioMuta; MYOM2; -.
DR   DMDM; 224471842; -.
DR   MassIVE; P54296; -.
DR   MaxQB; P54296; -.
DR   PaxDb; 9606-ENSP00000262113; -.
DR   PeptideAtlas; P54296; -.
DR   ProteomicsDB; 56680; -.
DR   Pumba; P54296; -.
DR   Antibodypedia; 940; 171 antibodies from 26 providers.
DR   DNASU; 9172; -.
DR   Ensembl; ENST00000262113.9; ENSP00000262113.4; ENSG00000036448.10.
DR   Ensembl; ENST00000616680.2; ENSP00000478322.1; ENSG00000274137.2.
DR   GeneID; 9172; -.
DR   KEGG; hsa:9172; -.
DR   MANE-Select; ENST00000262113.9; ENSP00000262113.4; NM_003970.4; NP_003961.3.
DR   UCSC; uc033azy.2; human.
DR   AGR; HGNC:7614; -.
DR   CTD; 9172; -.
DR   DisGeNET; 9172; -.
DR   GeneCards; MYOM2; -.
DR   HGNC; HGNC:7614; MYOM2.
DR   HPA; ENSG00000036448; Group enriched (heart muscle, skeletal muscle).
DR   MIM; 603509; gene.
DR   neXtProt; NX_P54296; -.
DR   OpenTargets; ENSG00000036448; -.
DR   PharmGKB; PA31419; -.
DR   VEuPathDB; HostDB:ENSG00000036448; -.
DR   eggNOG; ENOG502QS6D; Eukaryota.
DR   GeneTree; ENSGT00940000157057; -.
DR   HOGENOM; CLU_004753_1_0_1; -.
DR   InParanoid; P54296; -.
DR   OMA; ASFRFVI; -.
DR   OrthoDB; 198115at2759; -.
DR   PhylomeDB; P54296; -.
DR   TreeFam; TF331825; -.
DR   PathwayCommons; P54296; -.
DR   SignaLink; P54296; -.
DR   SIGNOR; P54296; -.
DR   BioGRID-ORCS; 9172; 7 hits in 1145 CRISPR screens.
DR   ChiTaRS; MYOM2; human.
DR   GeneWiki; MYOM2; -.
DR   GenomeRNAi; 9172; -.
DR   Pharos; P54296; Tbio.
DR   PRO; PR:P54296; -.
DR   Proteomes; UP000005640; Chromosome 8.
DR   RNAct; P54296; Protein.
DR   Bgee; ENSG00000036448; Expressed in apex of heart and 104 other cell types or tissues.
DR   ExpressionAtlas; P54296; baseline and differential.
DR   GO; GO:0031430; C:M band; IEA:UniProtKB-SubCell.
DR   GO; GO:0005739; C:mitochondrion; HDA:UniProtKB.
DR   GO; GO:0032982; C:myosin filament; IEA:UniProtKB-KW.
DR   GO; GO:0019900; F:kinase binding; IPI:CAFA.
DR   GO; GO:0008307; F:structural constituent of muscle; TAS:ProtInc.
DR   GO; GO:0002074; P:extraocular skeletal muscle development; IEA:Ensembl.
DR   GO; GO:0006936; P:muscle contraction; TAS:ProtInc.
DR   GO; GO:0045214; P:sarcomere organization; IMP:FlyBase.
DR   CDD; cd00063; FN3; 5.
DR   CDD; cd00096; Ig; 2.
DR   CDD; cd05891; IgI_M-protein_C; 1.
DR   CDD; cd20951; IgI_titin_I1-like; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 12.
DR   InterPro; IPR003961; FN3_dom.
DR   InterPro; IPR036116; FN3_sf.
DR   InterPro; IPR007110; Ig-like_dom.
DR   InterPro; IPR036179; Ig-like_dom_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR013098; Ig_I-set.
DR   InterPro; IPR003599; Ig_sub.
DR   InterPro; IPR003598; Ig_sub2.
DR   PANTHER; PTHR13817:SF73; PROTEIN SIDEKICK HOMOLOG; 1.
DR   PANTHER; PTHR13817; TITIN; 1.
DR   Pfam; PF00041; fn3; 5.
DR   Pfam; PF07679; I-set; 3.
DR   PRINTS; PR00014; FNTYPEIII.
DR   SMART; SM00060; FN3; 5.
DR   SMART; SM00409; IG; 5.
DR   SMART; SM00408; IGc2; 3.
DR   SUPFAM; SSF49265; Fibronectin type III; 3.
DR   SUPFAM; SSF48726; Immunoglobulin; 6.
DR   PROSITE; PS50853; FN3; 5.
DR   PROSITE; PS50835; IG_LIKE; 5.
DR   Genevisible; P54296; HS.
PE   1: Evidence at protein level;
KW   Cytoplasm; Immunoglobulin domain; Muscle protein; Reference proteome;
KW   Repeat; Thick filament.
FT   CHAIN           1..1465
FT                   /note="Myomesin-2"
FT                   /id="PRO_0000072686"
FT   DOMAIN          154..245
FT                   /note="Ig-like C2-type 1"
FT   DOMAIN          266..371
FT                   /note="Ig-like C2-type 2"
FT   DOMAIN          385..480
FT                   /note="Fibronectin type-III 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT   DOMAIN          513..608
FT                   /note="Fibronectin type-III 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT   DOMAIN          614..707
FT                   /note="Fibronectin type-III 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT   DOMAIN          710..812
FT                   /note="Fibronectin type-III 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT   DOMAIN          815..912
FT                   /note="Fibronectin type-III 5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT   DOMAIN          904..1002
FT                   /note="Ig-like C2-type 3"
FT   DOMAIN          1130..1211
FT                   /note="Ig-like C2-type 4"
FT   DOMAIN          1345..1434
FT                   /note="Ig-like C2-type 5"
FT   REGION          38..61
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1442..1465
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         81
FT                   /note="E -> K (in dbSNP:rs35985218)"
FT                   /id="VAR_033613"
FT   VARIANT         182
FT                   /note="T -> M (in dbSNP:rs17064618)"
FT                   /id="VAR_033614"
FT   VARIANT         321
FT                   /note="V -> L (in dbSNP:rs2272720)"
FT                   /evidence="ECO:0000269|PubMed:7505783"
FT                   /id="VAR_033615"
FT   VARIANT         363
FT                   /note="V -> I (in dbSNP:rs34316994)"
FT                   /id="VAR_033616"
FT   VARIANT         601
FT                   /note="S -> Y (in dbSNP:rs36089594)"
FT                   /id="VAR_033617"
FT   VARIANT         701
FT                   /note="V -> I (in dbSNP:rs35335787)"
FT                   /id="VAR_033618"
FT   VARIANT         776
FT                   /note="T -> M (in dbSNP:rs2294066)"
FT                   /evidence="ECO:0000269|PubMed:15489334"
FT                   /id="VAR_020083"
FT   VARIANT         869
FT                   /note="S -> N (in dbSNP:rs968381)"
FT                   /id="VAR_054501"
FT   VARIANT         1022
FT                   /note="L -> F (in dbSNP:rs2280896)"
FT                   /id="VAR_020084"
FT   VARIANT         1168
FT                   /note="V -> A (in dbSNP:rs17854780)"
FT                   /evidence="ECO:0000269|PubMed:15489334,
FT                   ECO:0000269|PubMed:7505783"
FT                   /id="VAR_061320"
FT   VARIANT         1284
FT                   /note="E -> D (in dbSNP:rs34735757)"
FT                   /id="VAR_033619"
FT   CONFLICT        102..103
FT                   /note="QR -> HG (in Ref. 1; CAA48832)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        364
FT                   /note="S -> T (in Ref. 1; CAA48832)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        555
FT                   /note="S -> T (in Ref. 1; CAA48832)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        863
FT                   /note="N -> D (in Ref. 1; CAA48832)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1465 AA;  164869 MW;  E9573DA6E0076343 CRC64;
     MSLVTVPFYQ KRHRHFDQSY RNIQTRYLLD EYASKKRAST QASSQKSLSQ RSSSQRASSQ
     TSLGGTICRV CAKRVSTQED EEQENRSRYQ SLVAAYGEAK RQRFLSELAH LEEDVHLARS
     QARDKLDKYA IQQMMEDKLA WERHTFEERI SRAPEILVRL RSHTVWERMS VKLCFTVQGF
     PTPVVQWYKD GSLICQAAEP GKYRIESNYG VHTLEINRAD FDDTATYSAV ATNAHGQVST
     NAAVVVRRFR GDEEPFRSVG LPIGLPLSSM IPYTHFDVQF LEKFGVTFRR EGETVTLKCT
     MLVTPDLKRV QPRAEWYRDD VLLKESKWTK MFFGEGQASL SFSHLHKDDE GLYTLRIVSR
     GGVSDHSAFL FVRDADPLVT GAPGAPMDLQ CHDANRDYVI VTWKPPNTTT ESPVMGYFVD
     RCEVGTNNWV QCNDAPVKIC KYPVTGLFEG RSYIFRVRAV NSAGISRPSR VSDAVAALDP
     LDLRRLQAVH LEGEKEIAIY QDDLEGDAQV PGPPTGVHAS EISRNYVVLS WEPPTPRGKD
     PLMYFIEKSV VGSGSWQRVN AQTAVRSPRY AVFDLMEGKS YVFRVLSANR HGLSEPSEIT
     SPIQAQDVTV VPSAPGRVLA SRNTKTSVVV QWDRPKHEED LLGYYVDCCV AGTNLWEPCN
     HKPIGYNRFV VHGLTTGEQY IFRVKAVNAV GMSENSQESD VIKVQAALTV PSHPYGITLL
     NCDGHSMTLG WKVPKFSGGS PILGYYLDKR EVHHKNWHEV NSSPSKPTIL TVDGLTEGSL
     YEFKIAAVNL AGIGEPSDPS EHFKCEAWTM PEPGPAYDLT FCEVRDTSLV MLWKAPVYSG
     SSPVSGYFVD FREEDAGEWI TVNQTTTASR YLKVSDLQQG KTYVFRVRAV NANGVGKPSD
     TSEPVLVEAR PGTKEISAGV DEQGNIYLGF DCQEMTDASQ FTWCKSYEEI SDDERFKIET
     VGDHSKLYLK NPDKEDLGTY SVSVSDTDGV SSSFVLDPEE LERLMALSNE IKNPTIPLKS
     ELAYEIFDKG RVRFWLQAEH LSPDASYRFI INDREVSDSE IHRIKCDKAT GIIEMVMDRF
     SIENEGTYTV QIHDGKAKSQ SSLVLIGDAF KTVLEEAEFQ RKEFLRKQGP HFAEYLHWDV
     TEECEVRLVC KVANTKKETV FKWLKDDVLY ETETLPNLER GICELLIPKL SKKDHGEYKA
     TLKDDRGQDV SILEIAGKVY DDMILAMSRV CGKSASPLKV LCTPEGIRLQ CFMKYFTDEM
     KVNWCHKDAK ISSSEHMRIG GSEEMAWLQI CEPTEKDKGK YTFEIFDGKD NHQRSLDLSG
     QAFDEAFAEF QQFKAAAFAE KNRGRLIGGL PDVVTIMEGK TLNLTCTVFG NPDPEVIWFK
     NDQDIQLSEH FSVKVEQAKY VSMTIKGVTS EDSGKYSINI KNKYGGEKID VTVSVYKHGE
     KIPDMAPPQQ AKPKLIPASA SAAGQ
//