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Protein

Voltage-dependent calcium channel subunit alpha-2/delta-1

Gene

Cacna2d1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Plays an important role in excitation-contraction coupling (By similarity).By similarity

Miscellaneous

Binds gabapentin, an antiepileptic drug.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi258Divalent metal cationBy similarity1
Metal bindingi260Divalent metal cationBy similarity1
Metal bindingi262Divalent metal cationBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • voltage-gated calcium channel activity Source: RGD

GO - Biological processi

  • calcium ion transmembrane transport Source: BHF-UCL
  • calcium ion transmembrane transport via high voltage-gated calcium channel Source: BHF-UCL
  • calcium ion transport Source: RGD
  • calcium ion transport into cytosol Source: BHF-UCL
  • positive regulation of high voltage-gated calcium channel activity Source: BHF-UCL
  • regulation of membrane repolarization during action potential Source: BHF-UCL

Keywordsi

Molecular functionCalcium channel, Ion channel, Voltage-gated channel
Biological processCalcium transport, Ion transport, Transport
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent calcium channel subunit alpha-2/delta-1
Alternative name(s):
Voltage-gated calcium channel subunit alpha-2/delta-1
Cleaved into the following 2 chains:
Gene namesi
Name:Cacna2d1
Synonyms:Cacnl2a, Cchl2a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2247 Cacna2d1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 1061ExtracellularSequence analysisAdd BLAST1037
Transmembranei1062 – 1082HelicalSequence analysisAdd BLAST21
Topological domaini1083 – 1091CytoplasmicSequence analysis9

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3420

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24By similarityAdd BLAST24
ChainiPRO_000030463525 – 1091Voltage-dependent calcium channel subunit alpha-2/delta-1Add BLAST1067
ChainiPRO_000000500525 – 944Voltage-dependent calcium channel subunit alpha-2-1By similarityAdd BLAST920
ChainiPRO_0000005006945 – 1091Voltage-dependent calcium channel subunit delta-1By similarityAdd BLAST147

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi92N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei119PhosphoserineCombined sources1
Glycosylationi136N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi184N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi323N-linked (GlcNAc...) asparagine; alternateSequence analysis1
Glycosylationi323N-linked (HexNAc...) asparagine; alternateCombined sources1
Glycosylationi347N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi403 ↔ 1047Interchain (between alpha-2-1 and delta-1 chains)By similarity
Glycosylationi593N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi769N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi876N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi973N-linked (HexNAc...) asparagineCombined sources1

Post-translational modificationi

Proteolytically processed into subunits alpha-2-1 and delta-1 that are disulfide-linked.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP54290
PRIDEiP54290

PTM databases

iPTMnetiP54290
PhosphoSitePlusiP54290
UniCarbKBiP54290

Interactioni

Subunit structurei

Dimer formed of alpha-2-1 and delta-1 chains; disulfide-linked. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1 (CACNA1), alpha-2 (CACNA2D), beta (CACNB) and delta (CACNA2D) subunits in a 1:1:1:1 ratio (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
THBS2P354422EBI-2466294,EBI-2466249From Homo sapiens.

Protein-protein interaction databases

IntActiP54290, 3 interactors
STRINGi10116.ENSRNOP00000034572

Chemistry databases

BindingDBiP54290

Structurei

3D structure databases

ProteinModelPortaliP54290
SMRiP54290
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini252 – 429VWFAPROSITE-ProRule annotationAdd BLAST178
Domaini445 – 536CacheAdd BLAST92

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi258 – 262MIDAS-like motif5

Domaini

The MIDAS-like motif in the VWFA domain binds divalent metal cations and is required to promote trafficking of the alpha-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2353 Eukaryota
ENOG410XPDX LUCA
HOGENOMiHOG000004860
HOVERGENiHBG057779
InParanoidiP54290
PhylomeDBiP54290

Family and domain databases

Gene3Di3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR013680 VDCC_a2/dsu
IPR013608 VWA_N
IPR002035 VWF_A
IPR036465 vWFA_dom_sf
PfamiView protein in Pfam
PF08473 VGCC_alpha2, 1 hit
PF00092 VWA, 1 hit
PF08399 VWA_N, 1 hit
SMARTiView protein in SMART
SM00327 VWA, 1 hit
SUPFAMiSSF53300 SSF53300, 1 hit
PROSITEiView protein in PROSITE
PS50234 VWFA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: 2 isoforms are produced.
Isoform 1 (identifier: P54290-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAGCLLALT LTLFQSWLIG PSSEEPFPSP VTIKSWVDKM QEDLVTLAKT
60 70 80 90 100
ASGVTQLADI YEKYQDLYTV EPNNARQLVE IAARDIEKLL SNRSKALVRL
110 120 130 140 150
AMEAEKVQAA HQWREDFASN EVVYYNAKDD LDPERNESES GSQRIKPVFI
160 170 180 190 200
EDANFGRQIS YQHAAVHIPT DIYEGSTIVL NELNWTSALD EVFKRNRDED
210 220 230 240 250
PTLLWQVFAA DRLARYYPAS PWVDNSRTPN KIDLYDVRRR PWYIQGAASP
260 270 280 290 300
KDMLILVDVS GSVSGLTLKL IRTSVSEMLE TLSDDDFVNV ASFNSNAQDV
310 320 330 340 350
SCFQHLVQAN VRNKKVLKDA VNNITAKGIT DYKKGFTFAF EQLLNYNVSR
360 370 380 390 400
ANCNKIIMLF TDGGEERAQE IFAKYNKDKK VRVFTFSVGQ HNYDRGPIQW
410 420 430 440 450
MACENKGYYY EIPSIGAIRI NTQEYLDVLG RPMVLAGDKA KQVQWTNVYL
460 470 480 490 500
DALELGLVIT GTLPVFNVTG QSENKTNLKN QLILGVMGVD VSLEDIKRLT
510 520 530 540 550
PRFTLCPNGY YFAIDPNGYV LLHPNLQPKN PKSQEPVTLD FLDAELENDI
560 570 580 590 600
KVEIRNKMID GESGEKTFRT LVKSQDERYI DKGNRTYTWT PVNGTDYRYL
610 620 630 640 650
ALVLPTYSFY YIKAKIEETI TQARSKKGKM KDSETLKPDN FEESGYTFIA
660 670 680 690 700
PREYCNDLKP SDNNTEFLLN FNEFIDRKTP NNPSCNTDLI NRILLDAGFT
710 720 730 740 750
NELVQNYWSK QKNIKGVKAR FVVTDGGITR VYPKEAGENW QENPETYEDS
760 770 780 790 800
FYKRSLDNDN YVFTAPYFNK SGPGAYESGI MVSKAVELYI QGKLLKPAVV
810 820 830 840 850
GIKIDVNSWI ENFTKTSIRD PCAGPVCDCK RNSDVMDCVI LDDGGFLLMA
860 870 880 890 900
NHDDYTNQIG RFFGEIDPRM MRHLVNISLY AFNKSYDYQS VCDPGAAPKQ
910 920 930 940 950
GAGHRSAYVP SITDILQIGW WATAAAWSIL QQLLLSLTFP RLLEAVEMEE
960 970 980 990 1000
DDFTASLSKQ SCITEQTQYF FKNDTKSFSG LLDCGNCSRI FHVEKLMNTN
1010 1020 1030 1040 1050
LVFIMVESKG TCPCDTRLLM QAEQTSDGPD PCDMVKQPRY RKGPDVCFDN
1060 1070 1080 1090
NVLEDYTDCG GVSGLNPSLW SIFGLQFILL WLVSGSRHYL W
Length:1,091
Mass (Da):123,823
Last modified:October 1, 1996 - v1
Checksum:i7054907D9D343B34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86621 mRNA Translation: AAA41088.1
PIRiA44147
UniGeneiRn.1110
Rn.11276

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCA2D1_RAT
AccessioniPrimary (citable) accession number: P54290
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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