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Protein

Voltage-dependent L-type calcium channel subunit beta-2

Gene

CACNB2

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei521Required for CaMK2D-bindingBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionCalcium channel, Ion channel, Voltage-gated channel
Biological processCalcium transport, Ion transport, Transport
LigandCalcium

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent L-type calcium channel subunit beta-2
Short name:
CAB2
Alternative name(s):
Calcium channel voltage-dependent subunit beta 2
Gene namesi
Name:CACNB2
Synonyms:CACNLB2
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3638171.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001440531 – 632Voltage-dependent L-type calcium channel subunit beta-2Add BLAST632

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei176PhosphoserineBy similarity1
Modified residuei179PhosphoserineBy similarity1
Modified residuei190PhosphoserineBy similarity1
Modified residuei522PhosphoserineBy similarity1
Modified residuei526Phosphothreonine; by CaMK2DBy similarity1

Post-translational modificationi

Regulated through phosphorylation at Thr-526 by CaMK2D.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP54288.

PTM databases

iPTMnetiP54288.

Expressioni

Tissue specificityi

Predominantly expressed in heart, aorta and brain.

Interactioni

Subunit structurei

Component of a calcium channel complex consisting of a pore-forming alpha subunit (CACNA1S) and the ancillary subunits CACNB1 or CACNB2, CACNG1 and CACNA2D1 (PubMed:15134636). The channel complex contains alpha, beta, gamma and delta subunits in a 1:1:1:1 ratio, i.e. it contains either CACNB1 or CACNB2 (By similarity). Interacts with CACNA1C (PubMed:22649239). Interacts with RRAD. Interaction with RRAD regulates the trafficking of CACNA1C to the cell membrane. Interacts with TMIGD2 (By similarity). Interacts with CAMK2D. Interacts with CBARP (By similarity).By similarity1 Publication1 Publication

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000021223.

Structurei

Secondary structure

1632
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi64 – 66Combined sources3
Helixi67 – 81Combined sources15
Beta strandi83 – 85Combined sources3
Beta strandi89 – 95Combined sources7
Helixi101 – 103Combined sources3
Beta strandi119 – 125Combined sources7
Beta strandi127 – 137Combined sources11
Beta strandi143 – 146Combined sources4
Helixi148 – 161Combined sources14
Beta strandi220 – 224Combined sources5
Beta strandi230 – 233Combined sources4
Beta strandi246 – 250Combined sources5
Beta strandi256 – 259Combined sources4
Helixi267 – 283Combined sources17
Turni284 – 286Combined sources3
Beta strandi287 – 293Combined sources7
Helixi297 – 299Combined sources3
Helixi302 – 305Combined sources4
Helixi312 – 315Combined sources4
Helixi323 – 336Combined sources14
Turni337 – 339Combined sources3
Beta strandi342 – 347Combined sources6
Helixi353 – 355Combined sources3
Turni356 – 358Combined sources3
Beta strandi364 – 368Combined sources5
Helixi373 – 381Combined sources9
Helixi385 – 388Combined sources4
Helixi391 – 403Combined sources13
Helixi406 – 408Combined sources3
Beta strandi410 – 413Combined sources4
Helixi418 – 436Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T3LX-ray2.20A51-163[»]
A229-448[»]
1T3SX-ray2.30A51-163[»]
A229-422[»]
4DEXX-ray2.00A51-163[»]
A229-448[»]
4DEYX-ray1.95A51-163[»]
A229-448[»]
ProteinModelPortaliP54288.
SMRiP54288.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54288.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 147SH3PROSITE-ProRule annotationAdd BLAST62

Sequence similaritiesi

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG3812. Eukaryota.
ENOG410XRDI. LUCA.
HOGENOMiHOG000230979.
HOVERGENiHBG050765.
InParanoidiP54288.
KOiK04863.

Family and domain databases

CDDicd12040. SH3_CACNB2. 1 hit.
InterProiView protein in InterPro
IPR035605. CACNB2_SH3.
IPR008145. GK/Ca_channel_bsu.
IPR027417. P-loop_NTPase.
IPR001452. SH3_domain.
IPR005444. VDCC_L_b2su.
IPR000584. VDCC_L_bsu.
PANTHERiPTHR11824. PTHR11824. 1 hit.
PTHR11824:SF17. PTHR11824:SF17. 1 hit.
PfamiView protein in Pfam
PF00625. Guanylate_kin. 1 hit.
PF12052. VGCC_beta4Aa_N. 1 hit.
PRINTSiPR01626. LCACHANNELB.
PR01628. LCACHANNELB2.
SMARTiView protein in SMART
SM00072. GuKc. 1 hit.
SM00326. SH3. 1 hit.
SUPFAMiSSF50044. SSF50044. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS50002. SH3. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2B (identifier: P54288-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNQASGLDLL KISYGKGARR KNRFKGSDGS TSSDTTSNSF VRQGSADSYT
60 70 80 90 100
SRPSDSDVSL EEDREAVRRE AERQAQAQLE KAKTKPVAFA VRTNVSYSAA
110 120 130 140 150
HEDDVPVPGM AISFEAKDFL HVKEKFNNDW WIGRLVKEGC EIGFIPSPVK
160 170 180 190 200
LENMRLQHEQ RAKQGKFYSS KSGGNSSSSL GDIVPSSRKS TPPSSAIDID
210 220 230 240 250
ATGLDAEEND IPANHRSPKP SANSVTSPHS KEKRMPFFKK TEHTPPYDVV
260 270 280 290 300
PSMRPVVLVG PSLKGYEVTD MMQKALFDFL KHRFEGRISI TRVTADISLA
310 320 330 340 350
KRSVLNNPSK HAIIERSNTR SSLAEVQSEI ERIFELARTL QLVVLDADTI
360 370 380 390 400
NHPAQLSKTS LAPIVVYVKI SSPKVLQRLI KSRGKSQAKH LNVQMVAADK
410 420 430 440 450
LAQCPPELFD VILDENQLED ACEHLADYLE AYWKATHPPS SNLPNPLLSR
460 470 480 490 500
TLATSALPVS PTLASNSQGS QGDQRTDRSA PARSASQAEE EPCLEPAKKS
510 520 530 540 550
QHRSSSSAPH HNHRSGTSRG LSRQETFDSE TQESRDSAYV EPKEDYSHEH
560 570 580 590 600
VDHYAPHRDH NHRDETHRSS DHRHRETRHR SRDMDREQDH NECNKQRSRH
610 620 630
KSKDRYCDKD GEVISKKRNE AGEWNRDVYI RQ
Length:632
Mass (Da):70,944
Last modified:October 1, 1996 - v1
Checksum:iEB6873D1F8DEB5D4
GO
Isoform 2A (identifier: P54288-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: MNQASGLDLLKISYGKGARRKNRFKGSDGSTSSDTTSNSFVRQ → MLDRHLAAPHTQGLVLE

Show »
Length:606
Mass (Da):68,178
Checksum:i55534DED1FD2F804
GO
Isoform 2C (identifier: P54288-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     197-240: IDIDATGLDAEENDIPANHRSPKPSANSVTSPHSKEKRMPFFKK → KQKQKS

Show »
Length:594
Mass (Da):66,812
Checksum:i3E0705B34B1B7C65
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0006321 – 43MNQAS…SFVRQ → MLDRHLAAPHTQGLVLE in isoform 2A. 1 PublicationAdd BLAST43
Alternative sequenceiVSP_000633197 – 240IDIDA…PFFKK → KQKQKS in isoform 2C. 1 PublicationAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64298 mRNA. Translation: CAA45576.1.
X64297 mRNA. Translation: CAA45575.1.
X64299 mRNA. Translation: CAA45577.1.
PIRiS21046.
S21048.
RefSeqiNP_001075865.1. NM_001082396.1. [P54288-1]
XP_017203069.1. XM_017347580.1. [P54288-3]
UniGeneiOcu.2071.

Genome annotation databases

GeneIDi100009277.
KEGGiocu:100009277.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCACB2_RABIT
AccessioniPrimary (citable) accession number: P54288
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: August 30, 2017
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families