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Protein

Voltage-dependent L-type calcium channel subunit beta-3

Gene

Cacnb3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.1 Publication

GO - Molecular functioni

  • high voltage-gated calcium channel activity Source: GO_Central
  • protein kinase binding Source: RGD
  • voltage-gated calcium channel activity Source: RGD

GO - Biological processi

  • calcium ion transmembrane transport Source: BHF-UCL
  • calcium ion transmembrane transport via high voltage-gated calcium channel Source: UniProtKB
  • calcium ion transport Source: RGD
  • calcium ion transport into cytosol Source: BHF-UCL
  • chemical synaptic transmission Source: GO_Central
  • neuromuscular junction development Source: GO_Central
  • protein localization to plasma membrane Source: BHF-UCL
  • regulation of membrane repolarization during action potential Source: BHF-UCL
  • regulation of voltage-gated calcium channel activity Source: GO_Central
  • T cell receptor signaling pathway Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-RNO-112308. Depolarization of the Presynaptic Terminal Triggers the Opening of Calcium Channels.
R-RNO-400042. Adrenaline,noradrenaline inhibits insulin secretion.
R-RNO-422356. Regulation of insulin secretion.
R-RNO-5576892. Phase 0 - rapid depolarisation.
R-RNO-5576893. Phase 2 - plateau phase.
R-RNO-5576894. Phase 1 - inactivation of fast Na+ channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent L-type calcium channel subunit beta-3
Short name:
CAB3
Alternative name(s):
Calcium channel voltage-dependent subunit beta 3
Gene namesi
Name:Cacnb3
Synonyms:Cacnlb3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi2248. Cacnb3.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • L-type voltage-gated calcium channel complex Source: UniProtKB
  • voltage-gated calcium channel complex Source: RGD
Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3137270.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001440591 – 484Voltage-dependent L-type calcium channel subunit beta-3Add BLAST484

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei152PhosphoserineCombined sources1
Modified residuei393PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP54287.
PRIDEiP54287.

PTM databases

iPTMnetiP54287.
PhosphoSitePlusiP54287.

Expressioni

Gene expression databases

BgeeiENSRNOG00000012489.
GenevisibleiP54287. RN.

Interactioni

Subunit structurei

The L-type calcium channel is composed of four subunits: alpha-1, alpha-2, beta and gamma (PubMed:15170217). Interacts with CACNA2D4. Interacts with FASLG (By similarity). Interacts with CBARP; prevents the interaction of CACNB3 with the alpha subunit CACNA1C thereby negatively regulating the activity of the corresponding calcium channel (PubMed:24751537).By similarity2 Publications

GO - Molecular functioni

  • protein kinase binding Source: RGD

Protein-protein interaction databases

BioGridi247336. 2 interactors.
DIPiDIP-59370N.
IntActiP54287. 1 interactor.
MINTiMINT-8407486.
STRINGi10116.ENSRNOP00000017490.

Structurei

Secondary structure

1484
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi34 – 38Combined sources5
Helixi40 – 55Combined sources16
Beta strandi62 – 68Combined sources7
Helixi72 – 74Combined sources3
Beta strandi92 – 98Combined sources7
Beta strandi100 – 110Combined sources11
Beta strandi116 – 119Combined sources4
Helixi121 – 131Combined sources11
Turni136 – 138Combined sources3
Beta strandi170 – 174Combined sources5
Beta strandi180 – 183Combined sources4
Helixi191 – 207Combined sources17
Turni208 – 210Combined sources3
Beta strandi211 – 217Combined sources7
Helixi221 – 223Combined sources3
Turni226 – 228Combined sources3
Helixi231 – 239Combined sources9
Helixi240 – 242Combined sources3
Helixi247 – 262Combined sources16
Beta strandi266 – 271Combined sources6
Helixi277 – 279Combined sources3
Turni280 – 282Combined sources3
Beta strandi288 – 292Combined sources5
Helixi297 – 305Combined sources9
Helixi309 – 312Combined sources4
Helixi315 – 326Combined sources12
Helixi330 – 332Combined sources3
Beta strandi334 – 337Combined sources4
Helixi342 – 360Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VYTX-ray2.60A/B16-366[»]
1VYUX-ray2.30A/B16-366[»]
ProteinModelPortaliP54287.
SMRiP54287.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54287.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 120SH3Add BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni195 – 345Mediates interaction with the alpha subunit1 PublicationAdd BLAST151

Sequence similaritiesi

Contains 1 SH3 domain.Curated

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG3812. Eukaryota.
ENOG410XRDI. LUCA.
GeneTreeiENSGT00390000002740.
HOGENOMiHOG000230979.
HOVERGENiHBG050765.
InParanoidiP54287.
KOiK04864.
OMAiNERNWQR.
OrthoDBiEOG091G09C1.
PhylomeDBiP54287.
TreeFamiTF316195.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR027417. P-loop_NTPase.
IPR001452. SH3_domain.
IPR008079. VDCC_L_b3su.
IPR000584. VDCC_L_bsu.
[Graphical view]
PANTHERiPTHR11824. PTHR11824. 1 hit.
PfamiPF00625. Guanylate_kin. 1 hit.
PF12052. VGCC_beta4Aa_N. 1 hit.
[Graphical view]
PRINTSiPR01626. LCACHANNELB.
PR01696. LCACHANNELB3.
SMARTiSM00072. GuKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P54287-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYDDSYVPGF EDSEAGSADS YTSRPSLDSD VSLEEDRESA RREVESQAQQ
60 70 80 90 100
QLERAKHKPV AFAVRTNVSY CGVLDEECPV QGSGVNFEAK DFLHIKEKYS
110 120 130 140 150
NDWWIGRLVK EGGDIAFIPS PQRLESIRLK QEQKARRSGN PSSLSDIGNR
160 170 180 190 200
RSPPPSLAKQ KQKQAEHVPP YDVVPSMRPV VLVGPSLKGY EVTDMMQKAL
210 220 230 240 250
FDFLKHRFDG RISITRVTAD LSLAKRSVLN NPGKRTIIER SSARSSIAEV
260 270 280 290 300
QSEIERIFEL AKSLQLVVLD ADTINHPAQL AKTSLAPIIV FVKVSSPKVL
310 320 330 340 350
QRLIRSRGKS QMKHLTVQMM AYDKLVQCPP ESFDVILDEN QLDDACEHLA
360 370 380 390 400
EYLEVYWRAT HHPAPGPGML GPPSAIPGLQ NQQLLGERGE EHSPLERDSL
410 420 430 440 450
MPSDEASESS RQAWTGSSQR SSRHLEEDYA DAYQDLYQPH RQHTSGLPSA
460 470 480
NGHDPQDRLL AQDSEHDHND RNWQRNRPWP KDSY
Length:484
Mass (Da):54,564
Last modified:October 1, 1996 - v1
Checksum:i18815828E9C5C9CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88751 mRNA. Translation: AAA18486.1.
PIRiA46608.
RefSeqiNP_036960.1. NM_012828.2.
UniGeneiRn.2808.

Genome annotation databases

EnsembliENSRNOT00000081206; ENSRNOP00000073026; ENSRNOG00000054274.
GeneIDi25297.
KEGGirno:25297.
UCSCiRGD:2248. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88751 mRNA. Translation: AAA18486.1.
PIRiA46608.
RefSeqiNP_036960.1. NM_012828.2.
UniGeneiRn.2808.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VYTX-ray2.60A/B16-366[»]
1VYUX-ray2.30A/B16-366[»]
ProteinModelPortaliP54287.
SMRiP54287.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247336. 2 interactors.
DIPiDIP-59370N.
IntActiP54287. 1 interactor.
MINTiMINT-8407486.
STRINGi10116.ENSRNOP00000017490.

Chemistry databases

ChEMBLiCHEMBL3137270.

PTM databases

iPTMnetiP54287.
PhosphoSitePlusiP54287.

Proteomic databases

PaxDbiP54287.
PRIDEiP54287.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000081206; ENSRNOP00000073026; ENSRNOG00000054274.
GeneIDi25297.
KEGGirno:25297.
UCSCiRGD:2248. rat.

Organism-specific databases

CTDi784.
RGDi2248. Cacnb3.

Phylogenomic databases

eggNOGiKOG3812. Eukaryota.
ENOG410XRDI. LUCA.
GeneTreeiENSGT00390000002740.
HOGENOMiHOG000230979.
HOVERGENiHBG050765.
InParanoidiP54287.
KOiK04864.
OMAiNERNWQR.
OrthoDBiEOG091G09C1.
PhylomeDBiP54287.
TreeFamiTF316195.

Enzyme and pathway databases

ReactomeiR-RNO-112308. Depolarization of the Presynaptic Terminal Triggers the Opening of Calcium Channels.
R-RNO-400042. Adrenaline,noradrenaline inhibits insulin secretion.
R-RNO-422356. Regulation of insulin secretion.
R-RNO-5576892. Phase 0 - rapid depolarisation.
R-RNO-5576893. Phase 2 - plateau phase.
R-RNO-5576894. Phase 1 - inactivation of fast Na+ channels.

Miscellaneous databases

EvolutionaryTraceiP54287.
PROiP54287.

Gene expression databases

BgeeiENSRNOG00000012489.
GenevisibleiP54287. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR027417. P-loop_NTPase.
IPR001452. SH3_domain.
IPR008079. VDCC_L_b3su.
IPR000584. VDCC_L_bsu.
[Graphical view]
PANTHERiPTHR11824. PTHR11824. 1 hit.
PfamiPF00625. Guanylate_kin. 1 hit.
PF12052. VGCC_beta4Aa_N. 1 hit.
[Graphical view]
PRINTSiPR01626. LCACHANNELB.
PR01696. LCACHANNELB3.
SMARTiSM00072. GuKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCACB3_RAT
AccessioniPrimary (citable) accession number: P54287
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.