P54284 (CACB3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Voltage-dependent L-type calcium channel subunit beta-3
Short name=CAB3

Alternative name(s):
Calcium channel voltage-dependent subunit beta 3

Gene names

Name:	CACNB3
Synonyms:	CACNLB3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.
Subunit structure	The L-type calcium channel is composed of four subunits: alpha-1, alpha-2, beta and gamma. Interacts with CACNA2D4. Interacts with FASLG. Ref.8 Ref.9
Tissue specificity	Expressed mostly in brain, smooth muscle and ovary.
Sequence similarities	Belongs to the calcium channel beta subunit family. Contains 1 SH3 domain.

Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Coding sequence diversity	Alternative splicing Polymorphism
Domain	SH3 domain
Ligand	Calcium
Molecular function	Calcium channel Ion channel Voltage-gated channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	T cell receptor signaling pathway Inferred from electronic annotation. Source: Compara axon guidance Traceable author statement. Source: Reactome membrane depolarization Traceable author statement. Source: Reactome synaptic transmission Traceable author statement. Source: Reactome
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: Compara cytosol Traceable author statement. Source: Reactome voltage-gated calcium channel complex Inferred from direct assay PubMed 11160515. Source: UniProtKB
Molecular_function	voltage-gated calcium channel activity Traceable author statement Ref.4. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 3A (identifier: P54284-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 3B (identifier: P54284-2)

The sequence of this isoform differs from the canonical sequence as follows:
     440-484: HRQHTSGLPSANGHDPQDRLLAQDSEHNHSDRNWQRNRPWPKDSY → QCQLLSLL

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	Voltage-dependent L-type calcium channel subunit beta-3		PRO_0000144056
Regions
	Domain	59 – 120	62	SH3
Natural variations
	Alternative sequence	440 – 484	45	HRQHT…PKDSY → QCQLLSLL in isoform 3B.		VSP_000634
	Natural variant	423	1	R → H. Corresponds to variant rs2229954 [ dbSNP | Ensembl ].		VAR_024384
Experimental info
	Sequence conflict	7	1	V → L in AAA19799. Ref.4
	Sequence conflict	32	1	Missing in AAA19799. Ref.4
	Sequence conflict	60	1	V → E in AAA19799. Ref.4
	Sequence conflict	320	1	Missing in AAA19799. Ref.4
	Sequence conflict	348	1	H → L in AAA19799. Ref.4
	Sequence conflict	421	1	S → T in AAA19799. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 3A [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 19CD3549F5A8E17A	FASTA	484	54,532
	10 20 30 40 50 60 MYDDSYVPGF EDSEAGSADS YTSRPSLDSD VSLEEDRESA RREVESQAQQ QLERAKHKPV 70 80 90 100 110 120 AFAVRTNVSY CGVLDEECPV QGSGVNFEAK DFLHIKEKYS NDWWIGRLVK EGGDIAFIPS 130 140 150 160 170 180 PQRLESIRLK QEQKARRSGN PSSLSDIGNR RSPPPSLAKQ KQKQAEHVPP YDVVPSMRPV 190 200 210 220 230 240 VLVGPSLKGY EVTDMMQKAL FDFLKHRFDG RISITRVTAD LSLAKRSVLN NPGKRTIIER 250 260 270 280 290 300 SSARSSIAEV QSEIERIFEL AKSLQLVVLD ADTINHPAQL AKTSLAPIIV FVKVSSPKVL 310 320 330 340 350 360 QRLIRSRGKS QMKHLTVQMM AYDKLVQCPP ESFDVILDEN QLEDACEHLA EYLEVYWRAT 370 380 390 400 410 420 HHPAPGPGLL GPPSAIPGLQ NQQLLGERGE EHSPLERDSL MPSDEASESS RQAWTGSSQR 430 440 450 460 470 480 SSRHLEEDYA DAYQDLYQPH RQHTSGLPSA NGHDPQDRLL AQDSEHNHSD RNWQRNRPWP KDSY
	Isoform 3B [UniParc]. Checksum: C2BCB90B9B4FEB3F	FASTA	447	50,106
	10 20 30 40 50 60 MYDDSYVPGF EDSEAGSADS YTSRPSLDSD VSLEEDRESA RREVESQAQQ QLERAKHKPV 70 80 90 100 110 120 AFAVRTNVSY CGVLDEECPV QGSGVNFEAK DFLHIKEKYS NDWWIGRLVK EGGDIAFIPS 130 140 150 160 170 180 PQRLESIRLK QEQKARRSGN PSSLSDIGNR RSPPPSLAKQ KQKQAEHVPP YDVVPSMRPV 190 200 210 220 230 240 VLVGPSLKGY EVTDMMQKAL FDFLKHRFDG RISITRVTAD LSLAKRSVLN NPGKRTIIER 250 260 270 280 290 300 SSARSSIAEV QSEIERIFEL AKSLQLVVLD ADTINHPAQL AKTSLAPIIV FVKVSSPKVL 310 320 330 340 350 360 QRLIRSRGKS QMKHLTVQMM AYDKLVQCPP ESFDVILDEN QLEDACEHLA EYLEVYWRAT 370 380 390 400 410 420 HHPAPGPGLL GPPSAIPGLQ NQQLLGERGE EHSPLERDSL MPSDEASESS RQAWTGSSQR 430 440 SSRHLEEDYA DAYQDLYQPQ CQLLSLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Murakami M., Klugbauer N., Flockerzi V. Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3A AND 3B).
[2]	"The structures of the human calcium channel alpha 1 subunit (CACNL1A2) and beta subunit (CACNLB3) genes." Yamada Y., Masuda K., Li Q., Ihara Y., Kubota A., Miura T., Nakamura K., Fujii Y., Seino S., Seino Y. Genomics 27:312-319(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Furneaux H.M. Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3A). Tissue: Fetal brain.
[4]	"Cloning, chromosomal location and functional expression of the human voltage-dependent calcium-channel beta 3 subunit." Collin T., Lory P., Taviaux S., Courtieu C., Guilbault P., Berta P., Nargeot J. Eur. J. Biochem. 220:257-262(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3A).
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3A). Tissue: Brain.
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3A). Tissue: Brain.
[8]	"Molecular cloning and characterization of the human voltage-gated calcium channel alpha(2)delta-4 subunit." Qin N., Yagel S., Momplaisir M.-L., Codd E.E., D'Andrea M.R. Mol. Pharmacol. 62:485-496(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CACNA2D4.
[9]	"Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening." Voss M., Lettau M., Janssen O. BMC Immunol. 10:53-53(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FASLG.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X76555 mRNA. Translation: CAA54055.1. X76556 mRNA. Translation: CAA54056.1. D43704 Genomic DNA. Translation: BAA07803.1. U07139 mRNA. Translation: AAA95958.1. L27584 mRNA. Translation: AAA19799.1. AK289709 mRNA. Translation: BAF82398.1. CH471111 Genomic DNA. Translation: EAW58008.1. BC041811 mRNA. Translation: AAH41811.1.
IPI	IPI00005567. IPI00031790.
PIR	S39315. S39316. S41211.
RefSeq	NP_000716.2. NM_000725.3.
UniGene	Hs.250712. Hs.737291.
3D structure databases
ProteinModelPortal	P54284.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-39138N.
IntAct	P54284. 2 interactions.
STRING	9606.ENSP00000301050.
Protein family/group databases
TCDB	8.A.22.3.1. Ca2+ channel auxiliary subunit beta types 1-4 (CCA-beta) family.
PTM databases
PhosphoSite	P54284.
Polymorphism databases
DMDM	1705683.
Proteomic databases
PaxDb	P54284.
PRIDE	P54284.
Protocols and materials databases
DNASU	784.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000301050; ENSP00000301050; ENSG00000167535.
GeneID	784.
KEGG	hsa:784.
UCSC	uc001rsk.2. human.
Organism-specific databases
CTD	784.
GeneCards	GC12P049212.
HGNC	HGNC:1403. CACNB3.
MIM	601958. gene.
neXtProt	NX_P54284.
PharmGKB	PA89.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG326500.
HOGENOM	HOG000230979.
HOVERGEN	HBG050765.
InParanoid	P54284.
KO	K04864.
OMA	LATPPNK.
OrthoDB	EOG469QTZ.
Enzyme and pathway databases
Reactome	REACT_111045. Developmental Biology. REACT_111217. Metabolism. REACT_13685. Neuronal System.
Gene expression databases
ArrayExpress	P54284.
Bgee	P54284.
CleanEx	HS_CACNB3.
Genevestigator	P54284.
GermOnline	ENSG00000167535. Homo sapiens.
Family and domain databases
InterPro	IPR008144. Guanylate_kin. IPR008145. Guanylate_kin/L-typ_Ca_channel. IPR001452. SH3_domain. IPR008079. VDCC_L_b3su. IPR000584. VDCC_L_bsu. [Graphical view]
PANTHER	PTHR11824. PTHR11824. 1 hit.
Pfam	PF00625. Guanylate_kin. 1 hit. PF12052. VGCC_beta4Aa_N. 1 hit. [Graphical view]
PRINTS	PR01626. LCACHANNELB. PR01696. LCACHANNELB3.
SMART	SM00072. GuKc. 1 hit. SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF50044. SH3. 1 hit.
PROSITE	PS50002. SH3. False negative. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00661. Verapamil.
GenomeRNAi	784.
NextBio	3190.
SOURCE	Search...

Entry information

Entry name

CACB3_HUMAN

Accession

Primary (citable) accession number: P54284
Secondary accession number(s): A8K0Z4, Q13913

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 126 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.