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P54282

- CAC1A_RAT

UniProt

P54282 - CAC1A_RAT

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Protein
Voltage-dependent P/Q-type calcium channel subunit alpha-1A
Gene
Cacna1a, Cach4, Cacn3, Cacnl1a4
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1A gives rise to P and/or Q-type calcium currents. P/Q-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by the funnel toxin (Ftx) and by the omega-agatoxin-IVA (omega-Aga-IVA). They are however insensitive to dihydropyridines (DHP), and omega-conotoxin-GVIA (omega-CTx-GVIA).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei320 – 3201Calcium ion selectivity and permeability By similarity
Sitei670 – 6701Calcium ion selectivity and permeability By similarity
Sitei1411 – 14111Calcium ion selectivity and permeability By similarity
Sitei1600 – 16001Binds to omega-Aga-IVA By similarity
Sitei1707 – 17071Calcium ion selectivity and permeability By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi1791 – 180212 By similarity
Add
BLAST

GO - Molecular functioni

  1. calmodulin binding Source: UniProtKB
  2. high voltage-gated calcium channel activity Source: RGD
  3. metal ion binding Source: UniProtKB-KW
  4. protein binding Source: UniProtKB
  5. voltage-gated calcium channel activity Source: UniProtKB

GO - Biological processi

  1. calcium ion import Source: RGD
  2. calcium ion transport Source: RGD
  3. membrane depolarization during action potential Source: RefGenome
  4. positive regulation of cytosolic calcium ion concentration Source: UniProtKB
  5. sensory perception of pain Source: RGD
  6. synaptic transmission Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

TCDBi1.A.1.11.8. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent P/Q-type calcium channel subunit alpha-1A
Alternative name(s):
Brain calcium channel I
Short name:
BI
Calcium channel, L type, alpha-1 polypeptide, isoform 4
Rat brain class A
Short name:
RBA-I
Voltage-gated calcium channel subunit alpha Cav2.1
Gene namesi
Name:Cacna1a
Synonyms:Cach4, Cacn3, Cacnl1a4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2244. Cacna1a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 100100Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei101 – 11919Helical; Name=S1 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini120 – 13819Extracellular Reviewed prediction
Add
BLAST
Transmembranei139 – 15618Helical; Name=S2 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini157 – 16812Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei169 – 18416Helical; Name=S3 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini185 – 1928Extracellular Reviewed prediction
Transmembranei193 – 21119Helical; Name=S4 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini212 – 23019Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei231 – 25020Helical; Name=S5 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini251 – 33787Extracellular Reviewed prediction
Add
BLAST
Transmembranei338 – 36225Helical; Name=S6 of repeat I; Reviewed prediction
Add
BLAST
Topological domaini363 – 489127Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei490 – 50920Helical; Name=S1 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini510 – 52314Extracellular Reviewed prediction
Add
BLAST
Transmembranei524 – 54320Helical; Name=S2 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini544 – 5518Cytoplasmic Reviewed prediction
Transmembranei552 – 57019Helical; Name=S3 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini571 – 58010Extracellular Reviewed prediction
Transmembranei581 – 59919Helical; Name=S4 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini600 – 61819Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei619 – 63820Helical; Name=S5 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini639 – 69153Extracellular Reviewed prediction
Add
BLAST
Transmembranei692 – 71625Helical; Name=S6 of repeat II; Reviewed prediction
Add
BLAST
Topological domaini717 – 1190474Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1191 – 121424Helical; Name=S1 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1215 – 123117Extracellular Reviewed prediction
Add
BLAST
Transmembranei1232 – 125120Helical; Name=S2 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1252 – 12587Cytoplasmic Reviewed prediction
Transmembranei1259 – 128224Helical; Name=S3 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1283 – 129311Extracellular Reviewed prediction
Add
BLAST
Transmembranei1294 – 131118Helical; Name=S4 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1312 – 133019Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1331 – 135020Helical; Name=S5 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1351 – 143787Extracellular Reviewed prediction
Add
BLAST
Transmembranei1438 – 146225Helical; Name=S6 of repeat III; Reviewed prediction
Add
BLAST
Topological domaini1463 – 151856Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1519 – 153719Helical; Name=S1 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1538 – 155114Extracellular Reviewed prediction
Add
BLAST
Transmembranei1552 – 157322Helical; Name=S2 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1574 – 15807Cytoplasmic Reviewed prediction
Transmembranei1581 – 160020Helical; Name=S3 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1601 – 16077Extracellular Reviewed prediction
Transmembranei1608 – 162619Helical; Name=S4 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1627 – 164519Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei1646 – 166520Helical; Name=S5 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1666 – 173772Extracellular Reviewed prediction
Add
BLAST
Transmembranei1738 – 176326Helical; Name=S6 of repeat IV; Reviewed prediction
Add
BLAST
Topological domaini1764 – 2212449Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. perikaryon Source: RGD
  2. plasma membrane Source: RGD
  3. protein complex Source: RGD
  4. voltage-gated calcium channel complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 22122212Voltage-dependent P/Q-type calcium channel subunit alpha-1A
PRO_0000053919Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi285 – 2851N-linked (GlcNAc...) Reviewed prediction
Modified residuei792 – 7921Phosphoserine By similarity
Glycosylationi1607 – 16071N-linked (GlcNAc...) Reviewed prediction
Modified residuei1773 – 17731Phosphoserine; by PKA Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP54282.
PRIDEiP54282.

PTM databases

PhosphoSiteiP54282.

Expressioni

Tissue specificityi

Brain specific. Purkinje cells contain predominantly P-type VSCC, the Q-type being a prominent calcium current in cerebellar granule cells. Also found in heart, in kidney distal convoluted tubule (DCT), and in pituitary.

Gene expression databases

GenevestigatoriP54282.

Interactioni

Subunit structurei

Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. Interact (via C-terminal CDB motif) with CABP1 in the pre- and postsynaptic membranes.

Binary interactionsi

WithEntry#Exp.IntActNotes
Rims1Q9JIR44EBI-3507416,EBI-3507436

Protein-protein interaction databases

DIPiDIP-29638N.
IntActiP54282. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP54282.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati87 – 365279I
Add
BLAST
Repeati475 – 719245II
Add
BLAST
Repeati1182 – 1465284III
Add
BLAST
Repeati1502 – 1765264IV
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni385 – 40218Binding to the beta subunit By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi13 – 208Poly-Gly
Compositional biasi729 – 7346Poly-Glu
Compositional biasi1155 – 11584Poly-Glu
Compositional biasi2162 – 217110Poly-His

Domaini

Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226.
HOGENOMiHOG000231530.
HOVERGENiHBG050763.
KOiK04344.
PhylomeDBiP54282.

Family and domain databases

Gene3Di1.20.120.350. 4 hits.
InterProiIPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR014873. VDCC_a1su_IQ.
IPR005448. VDCC_P/Q_a1su.
IPR002077. VDCCAlpha1.
[Graphical view]
PANTHERiPTHR10037:SF59. PTHR10037:SF59. 1 hit.
PfamiPF08763. Ca_chan_IQ. 1 hit.
PF00520. Ion_trans. 4 hits.
[Graphical view]
PRINTSiPR00167. CACHANNEL.
PR01632. PQVDCCALPHA1.
SMARTiSM01062. Ca_chan_IQ. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P54282-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MARFGDEMPG RYGAGGGGSG PAAGVVVGAA GGRGAGGSRQ GGQPGAQRMY     50
KQSMAQRART MALYNPIPVR QNCLTVNRSL FLFSEDNVVR KYAKKITEWP 100
PFEYMILATI IANCIVLALE QHLPDDDKTP MSERLDDTEP YFIGIFCFEA 150
GIKIVALGFA FHKGSYLRNG WNVMDFVVVL TGILATVGTE FDLRTLRAVR 200
VLRPLKLVSG IPSLQVVLKS IMKAMIPLLQ IGLLLFFAIL IFAIIGLEFY 250
MGKFHTTCFE EGTDDIQGES PAPCGTEEPA RTCPNGTKCQ PYWEGPNNGI 300
TQFDNILFAV LTVFQCITME GWTDLLYNSN DASGNTWNWL YFIPLIIIGS 350
FFMLNLVLGV LSGEFAKERE RVENRRAFLK LRRQQQIERE LNGYMEWISK 400
AEEVILAEDE TDVEQRHPFD GALRRATLKK SKTDLLNPEE AEDQLADIAS 450
VGSPFARASI KSAKLENSTF FHKKERRMRF YIRRMVKTQA FYWTVLSLVA 500
LNTLWLAIVH YNQPEWLSDF LYYAEFIFLG LFMSEMFIKM YGLGTRPYFH 550
SSFNCFDCGV IIGSIFEVIW AVIKPGTSFG ISVLRALRLL RIFKVTKYWA 600
SLRNLVVSLL NSMKSIISLL FLLFLFIVVF ALLGMQLFGG QFNFDEGTPP 650
TNFDTFPAAI MTVFQILTGE DWNEVMYDEI KSQGGVQGGM VFSIYFIVLT 700
LFGNYTLLNV FLAIAVDNLA NAQELTKDEQ EEEEAANQKL ALQKAKEVAE 750
VSPLSAANMS IAVKEQQKNQ KPAKSVWEQR TSEMRKQNLL ASREALYGDA 800
AERWPTTYAR PLRPDVKTHL DRPLVVDPQE NRNNNTNKSR APEALRQTAR 850
PRESARDPDA RRAWPSSPER APGREGPYGR ESEPQQREHA PPREHVPWDA 900
DPERAKAGDA PRRHTHRPVA EGEPRRHRAR RRPGDEPDDR PERRPRPRDA 950
TRPARAADGE GDDGERKRRH RHGPPAHDDR ERRHRRRKES QGSGVPMSGP 1000
NLSTTRPIQQ DLGRQDLPLA EDLDNMKNNK LATGEPASPH DSLGHSGLPP 1050
SPAKIGNSTN PGPALATNPQ NAASRRTPNN PGNPSNPGPP KTPENSLIVT 1100
NPSSTQPNSA KTARKPEHMA VEIPPACPPL NHTVVQVNKN ANPDPLPKKE 1150
EEKKEEEEAD PGEDGPKPMP PYSSMFILST TNPLRRLCHY ILNLRYFEMC 1200
ILMVIAMSSI ALAAEDPVQP NAPRNNVLRY FDYVFTGVFT FEMVIKMIDL 1250
GLVLHQGAYF RDLWNILDFI VVSGALVAFA FTGNSKGKDI NTIKSLRVLR 1300
VLRPLKTIKR LPKLKAVFDC VVNSLKNVFN ILIVYMLFMF IFAVVAVQLF 1350
KGKFFHCTDE SKEFERDCRG KYLLYEKNEV KARDREWKKY DFHYDNVLWA 1400
LLTLFTVSTG EGWPQVLKHS VDATFENQGP SPGYRMEMSI FYVVYFVVFP 1450
FFFVNIFVAL IIITFQEQGD KMMEEYSLEK NERACIDFAI SAKPLTRHMP 1500
QNKQSFQYRM WQFVVSPPFE YTIMAMIALN TIVLMMKFYG ASVAYENALR 1550
VFNIVFTSLF SLECVLKVMA FGILNYFRDA WNIFDFVTVL GSITDILVTE 1600
FGNNFINLSF LRLFRAARLI KLLRQGYTIR ILLWTFVQSF KALPYVCLLI 1650
AMLFFIYAII GMQVFGNIGI DGEDEDSDED EFQITEHNNF RTFFQALMLL 1700
FRSATGEAWH NIMLSCLSGK PCDKNSGIQK PECGNEFAYF YFVSFIFLCS 1750
FLMLNLFVAV IMDNFEYLTR DSSILGPHHL DEYVRVWAEY DPAACGRIHY 1800
KDMYSLLRVI SPPLGLGKKC PHRVACKRLL RMDLPVADDN TVHFNSTLMA 1850
LIRTALDIKI AKGGADKQQM DAELRKEMMA IWPNLSQKTL DLLVTPHKST 1900
DLTVGKIYAA MMIMEYYRQS KAKKLQAMRE EQNRTPLMFQ RMEPPSPTQE 1950
GGPSQNALPS TQLDPGGGLM AQESSMKESP SWVTQRAQEM FQKTGTWSPE 2000
RGPPIDMPNS QPNSQSVEMR EMGTDGYSDS EHYLPMEGQT RAASMPRLPA 2050
ENQRRRGRPR GNNLSTISDT SPMKRSASVL GPKARRLDDY SLERVPPEEN 2100
QRYHQRRRDR GHRTSERSLG RYTDVDTGLG TDLSMTTQSG DLPSKDRDQD 2150
RGRPKDRKHR PHHHHHHHHH HPPAPDRERY AQERPDTGRA RAREQRWSRS 2200
PSEGREHATH RQ 2212
Length:2,212
Mass (Da):251,527
Last modified:October 1, 1996 - v1
Checksum:iDFBD7A0F553AFA52
GO
Isoform 2 (identifier: P54282-2) [UniParc]FASTAAdd to Basket

Also known as: RKC8

The sequence of this isoform differs from the canonical sequence as follows:
     1602-1602: G → GNP

Show »
Length:2,214
Mass (Da):251,739
Checksum:iD2ED9869D22AF77F
GO
Isoform 3 (identifier: P54282-3)

Also known as: RBA-65

Sequence is not available
Length:
Mass (Da):
Isoform 4 (identifier: P54282-4)

Sequence is not available
Length:
Mass (Da):

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1602 – 16021G → GNP in isoform 2.
VSP_000881

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64373 mRNA. Translation: AAA40806.1.
M99222 mRNA. Translation: AAA40896.1.
PIRiA41098.
RefSeqiNP_037050.2. NM_012918.3.
UniGeneiRn.87769.

Genome annotation databases

GeneIDi25398.
KEGGirno:25398.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64373 mRNA. Translation: AAA40806.1 .
M99222 mRNA. Translation: AAA40896.1 .
PIRi A41098.
RefSeqi NP_037050.2. NM_012918.3.
UniGenei Rn.87769.

3D structure databases

ProteinModelPortali P54282.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-29638N.
IntActi P54282. 2 interactions.

Chemistry

BindingDBi P54282.
GuidetoPHARMACOLOGYi 532.

Protein family/group databases

TCDBi 1.A.1.11.8. the voltage-gated ion channel (vic) superfamily.

PTM databases

PhosphoSitei P54282.

Proteomic databases

PaxDbi P54282.
PRIDEi P54282.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 25398.
KEGGi rno:25398.

Organism-specific databases

CTDi 773.
RGDi 2244. Cacna1a.

Phylogenomic databases

eggNOGi COG1226.
HOGENOMi HOG000231530.
HOVERGENi HBG050763.
KOi K04344.
PhylomeDBi P54282.

Miscellaneous databases

NextBioi 13949997.
PROi P54282.

Gene expression databases

Genevestigatori P54282.

Family and domain databases

Gene3Di 1.20.120.350. 4 hits.
InterProi IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR014873. VDCC_a1su_IQ.
IPR005448. VDCC_P/Q_a1su.
IPR002077. VDCCAlpha1.
[Graphical view ]
PANTHERi PTHR10037:SF59. PTHR10037:SF59. 1 hit.
Pfami PF08763. Ca_chan_IQ. 1 hit.
PF00520. Ion_trans. 4 hits.
[Graphical view ]
PRINTSi PR00167. CACHANNEL.
PR01632. PQVDCCALPHA1.
SMARTi SM01062. Ca_chan_IQ. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Primary structure of a calcium channel that is highly expressed in the rat cerebellum."
    Starr T.V.B., Prystay W., Snutch T.P.
    Proc. Natl. Acad. Sci. U.S.A. 88:5621-5625(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Molecular characterization and nephron distribution of a family of transcripts encoding the pore-forming subunit of Ca2+ channels in the kidney."
    Yu A.S.L., Hebert S.C., Brenner B.M., Lytton J.
    Proc. Natl. Acad. Sci. U.S.A. 89:10494-10498(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1354-1659 (ISOFORM 2).
    Tissue: Kidney.
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1435-1667 (ISOFORM 3).
  4. "Differential modulation of Ca(v)2.1 channels by calmodulin and Ca2+-binding protein 1."
    Lee A., Westenbroek R.E., Haeseleer F., Palczewski K., Scheuer T., Catterall W.A.
    Nat. Neurosci. 5:210-217(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CABP1.

Entry informationi

Entry nameiCAC1A_RAT
AccessioniPrimary (citable) accession number: P54282
Secondary accession number(s): Q01541
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi