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Protein

Xylose isomerase

Gene

xylA

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-xylopyranose = D-xylulose.

Cofactori

Mn2+Note: Binds 2 manganese ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei991
Active sitei102By similarity1
Metal bindingi230Manganese 11
Metal bindingi266Manganese 11
Metal bindingi266Manganese 21
Metal bindingi294Manganese 11
Metal bindingi305Manganese 21
Metal bindingi307Manganese 21
Metal bindingi337Manganese 11

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism, Pentose shunt, Xylose metabolism

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Xylose isomerase (EC:5.3.1.5)
Gene namesi
Name:xylA
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001957661 – 441Xylose isomeraseAdd BLAST441

Interactioni

Subunit structurei

Homotetramer.

Structurei

Secondary structure

1441
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi22 – 25Combined sources4
Helixi37 – 41Combined sources5
Beta strandi43 – 46Combined sources4
Helixi47 – 52Combined sources6
Helixi67 – 70Combined sources4
Helixi73 – 91Combined sources19
Beta strandi94 – 99Combined sources6
Helixi100 – 103Combined sources4
Helixi110 – 128Combined sources19
Beta strandi134 – 139Combined sources6
Beta strandi143 – 145Combined sources3
Helixi146 – 148Combined sources3
Helixi158 – 178Combined sources21
Beta strandi181 – 185Combined sources5
Beta strandi190 – 193Combined sources4
Helixi195 – 197Combined sources3
Helixi200 – 220Combined sources21
Beta strandi225 – 229Combined sources5
Beta strandi235 – 242Combined sources8
Helixi245 – 254Combined sources10
Helixi258 – 260Combined sources3
Beta strandi261 – 266Combined sources6
Helixi267 – 272Combined sources6
Helixi277 – 286Combined sources10
Beta strandi290 – 294Combined sources5
Beta strandi302 – 304Combined sources3
Helixi313 – 325Combined sources13
Beta strandi334 – 336Combined sources3
Helixi348 – 373Combined sources26
Helixi376 – 384Combined sources9
Helixi386 – 388Combined sources3
Helixi391 – 398Combined sources8
Helixi403 – 411Combined sources9
Helixi423 – 435Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A0DX-ray3.00A/B/C/D2-441[»]
ProteinModelPortaliP54273.
SMRiP54273.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54273.

Family & Domainsi

Sequence similaritiesi

Belongs to the xylose isomerase family.Curated

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00455. Xylose_isom_A. 1 hit.
InterProiIPR013022. Xyl_isomerase-like_TIM-brl.
IPR013452. Xylose_isom_bac.
IPR001998. Xylose_isomerase.
[Graphical view]
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
PRINTSiPR00688. XYLOSISMRASE.
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR02630. xylose_isom_A. 1 hit.
PROSITEiPS51415. XYLOSE_ISOMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54273-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYFDNISTI AYEGPASKNP LAFKFYNPEE KVGDKTMEEH LRFSVAYWHT
60 70 80 90 100
FTGDGSDPFG AGNMIRPWNK YSGMDLAKAR VEAAFEFFEK LNIPFFCFHD
110 120 130 140 150
VDIAPEGETL KETYKNLDII VDMIEEYMKT SKTKLLWNTA NLFTHPRFVH
160 170 180 190 200
GAATSCNADV FAYAAAKVKK GLEIAKRLGA ENYVFWGGRE GYETLLNTDM
210 220 230 240 250
KLELDNLARF LHMAVDYAKE IGFDGQFLIE PKPKEPTKHQ YDFDVATALA
260 270 280 290 300
FLQTYGLKDY FKFNIEANHA TLAGHTFEHE LRVARIHGML GSVDANQGDM
310 320 330 340 350
LLGWDTDEFP TDLYSTTLAM YEILKNGGLG RGGLNFDAKV RRGSFEPEDL
360 370 380 390 400
FYAHIAGMDS FAVGLKVAHR LIEDRVFDEF IEERYKSYTE GIGREIVEGT
410 420 430 440
ADFHKLEAHA LQLGEIQNQS GRQERLKTLL NQYLLEVCAA R
Length:441
Mass (Da):50,142
Last modified:July 15, 1998 - v2
Checksum:i78BB39852919D017
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti248A → R in CAA66715 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98088 Genomic DNA. Translation: CAA66715.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98088 Genomic DNA. Translation: CAA66715.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A0DX-ray3.00A/B/C/D2-441[»]
ProteinModelPortaliP54273.
SMRiP54273.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP54273.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00455. Xylose_isom_A. 1 hit.
InterProiIPR013022. Xyl_isomerase-like_TIM-brl.
IPR013452. Xylose_isom_bac.
IPR001998. Xylose_isomerase.
[Graphical view]
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
PRINTSiPR00688. XYLOSISMRASE.
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR02630. xylose_isom_A. 1 hit.
PROSITEiPS51415. XYLOSE_ISOMERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiXYLA_GEOSE
AccessioniPrimary (citable) accession number: P54273
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 15, 1998
Last modified: November 2, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.