ID   G3PC_GRAVE              Reviewed;         335 AA.
AC   P54270;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   16-JUN-2009, entry version 56.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase, cytosolic;
DE            EC=1.2.1.12;
GN   Name=GAPC;
OS   Gracilaria verrucosa (Red alga).
OC   Eukaryota; Rhodophyta; Florideophyceae; Gracilariales; Gracilariaceae;
OC   Gracilaria.
OX   NCBI_TaxID=2777;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=96120861; PubMed=8590478; DOI=10.1007/BF00326430;
RA   Zhou Y.H., Ragan M.A.;
RT   "The nuclear gene and cDNAs encoding cytosolic glyceraldehyde-3-
RT   phosphate dehydrogenase from the marine red alga Gracilaria verrucosa:
RT   cloning, characterization and phylogenetic analysis.";
RL   Curr. Genet. 28:324-332(1995).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 1/5.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; L38661; AAB01379.1; -; Genomic_DNA.
DR   PIR; S59579; S59579.
DR   HSSP; P56649; 1DSS.
DR   SMR; P54270; 5-334.
DR   BRENDA; 1.2.1.12; 1477.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (p...; IEA:EC.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006096; P:glycolysis; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000173; GlycerAld_3-P_DH.
DR   InterPro; IPR006424; Glyceraldehyde-3-P_DH_1.
DR   PANTHER; PTHR10836; GAP_DH; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PIRSF; PIRSF000149; GAP_DH; 1.
DR   PRINTS; PR00078; G3PDHDRGNASE.
DR   TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR   PROSITE; PS00071; GAPDH; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Glycolysis; NAD; Oxidoreductase.
FT   CHAIN         1    335       Glyceraldehyde-3-phosphate dehydrogenase,
FT                                cytosolic.
FT                                /FTId=PRO_0000145601.
FT   NP_BIND      13     14       NAD (By similarity).
FT   REGION      151    153       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   REGION      211    212       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   ACT_SITE    152    152       Nucleophile (By similarity).
FT   BINDING      35     35       NAD (By similarity).
FT   BINDING      80     80       NAD; via carbonyl oxygen (By similarity).
FT   BINDING     182    182       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     234    234       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     316    316       NAD (By similarity).
FT   SITE        179    179       Activates thiol group during catalysis
FT                                (By similarity).
SQ   SEQUENCE   335 AA;  36144 MW;  5B97F5BF3D40ACB0 CRC64;
     MTVPQVGING FGRIGRLVLR AAIEKDTMSV VAINDPFIDL EYMAYMFKFD STHGRYAGSV
     ETKDGKLIVN GKSITIYGHR DPAEIPWAEA GADYVVESTG VFTLKEKAEK HFTGGAKKVI
     ISAPSKDAPM FVCGVNEDKY TPDLNVISNA SCTTNCLAPL VKVIHEKYGI EEGLMTTVHA
     TTATQKTVDG PSQKDWRGGR GAGANIIPSS TGAAKAVGKV LPELNGKLTG MAFRVPTSDV
     SVVDLTVRLA TETSYDDIKA TMKAAAEDSM KGILKYTEEA VVSTDFIHEE ASCVFDAGAG
     IMLNSRFCKL VAWYDNEWGY SNRVVDLIAH VAKLQ
//