Reviewed, UniProtKB/Swiss-Prot P54270 (G3PC_GRAVE)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase, cytosolic
    EC=1.2.1.12

Gene names

Name:

GAPC

Organism

Gracilaria verrucosa (Red alga)

Taxonomic identifier

2777 [NCBI]

Taxonomic lineage

Eukaryota › Rhodophyta › Florideophyceae › Gracilariales › Gracilariaceae › Gracilaria

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Glyceraldehyde-3-phosphate dehydrogenase, cytosolic		PRO_0000145601
Regions
	Nucleotide binding	13 – 14	2	NAD By similarity
	Region	151 – 153	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	211 – 212	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	152	1	Nucleophile By similarity
	Binding site	35	1	NAD By similarity
	Binding site	80	1	NAD; via carbonyl oxygen By similarity
	Binding site	182	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	234	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	316	1	NAD By similarity
	Site	179	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54270-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 5B97F5BF3D40ACB0	FASTA	335	36,144
	10 20 30 40 50 60 MTVPQVGING FGRIGRLVLR AAIEKDTMSV VAINDPFIDL EYMAYMFKFD STHGRYAGSV 70 80 90 100 110 120 ETKDGKLIVN GKSITIYGHR DPAEIPWAEA GADYVVESTG VFTLKEKAEK HFTGGAKKVI 130 140 150 160 170 180 ISAPSKDAPM FVCGVNEDKY TPDLNVISNA SCTTNCLAPL VKVIHEKYGI EEGLMTTVHA 190 200 210 220 230 240 TTATQKTVDG PSQKDWRGGR GAGANIIPSS TGAAKAVGKV LPELNGKLTG MAFRVPTSDV 250 260 270 280 290 300 SVVDLTVRLA TETSYDDIKA TMKAAAEDSM KGILKYTEEA VVSTDFIHEE ASCVFDAGAG 310 320 330 IMLNSRFCKL VAWYDNEWGY SNRVVDLIAH VAKLQ

References

[1]

"The nuclear gene and cDNAs encoding cytosolic glyceraldehyde-3-phosphate dehydrogenase from the marine red alga Gracilaria verrucosa: cloning, characterization and phylogenetic analysis." Zhou Y.H., Ragan M.A. Curr. Genet. 28:324-332(1995) [PubMed: 8590478] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L38661 Genomic DNA. Translation: AAB01379.1.
PIR	S59579.
3D structure databases
HSSP	HSSP built from PDB template 1DSS based on UniProtKB P56649.
SMR	P54270. Positions 5-334.
ModBase	Search...
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006424. Glyceraldehyde-3-P_DHase_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3PC_GRAVE

Accession

Primary (citable) accession number: P54270

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)