Reviewed, UniProtKB/Swiss-Prot P54267 (CHSG_ASPFU)

Names and origin

Protein names

Recommended name:
    Chitin synthase G
    EC=2.4.1.16
Alternative name(s):
    Chitin-UDP acetyl-glucosaminyl transferase G
    Class-III chitin synthase G

Gene names

Name:	chsG
ORF Names:	AFUA_3G14420

Organism

Aspergillus fumigatus (Sartorya fumigata) [Complete proteome]

Taxonomic identifier

5085 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	911 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis and required for normal hyphal growth and development. Ref.2
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family. Class III subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW chitin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 911	911	Chitin synthase G		PRO_0000193682
Regions
	Transmembrane	579 – 599	21	Potential
	Transmembrane	624 – 644	21	Potential
	Transmembrane	659 – 679	21	Potential
	Transmembrane	711 – 731	21	Potential
	Transmembrane	840 – 860	21	Potential
	Transmembrane	879 – 899	21	Potential
Experimental info
	Sequence conflict	334 – 337	4	PPVQ → LPSK in CAA63928. Ref.1
	Sequence conflict	537	1	R → C in AAB07679. Ref.2
	Sequence conflict	628 – 629	2	IV → MM in AAB07679. Ref.2
	Sequence conflict	892	1	F → N in AAB07679. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P54267-1 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: BC893F8BA00BE48D	FASTA	911	101,670
	10 20 30 40 50 60 MAYQGSGSHS PPHYDDNGHR LQDLPHGSYE EEASRGLLSH QQGPFTGPFD DPQQHGSSTT 70 80 90 100 110 120 RPVSGYSLSE TYAPEAAYHD PYTQPSPGSV YSAQSAENPA AAFGVPGRVA SPYARSDTSS 130 140 150 160 170 180 TEAWRQRQAP GGGPGGLRRY ATRKVKLVQG SVLSVDYPVP SAIQNAIQAK YRNDLEGGSE 190 200 210 220 230 240 EFTHMRYTAA TCDPNEFTLH NGYNLRPAMY NRHTELLIAI TYYNEDKTLT SRTLHGVMQN 250 260 270 280 290 300 IRDIVNLKKS EFWNKGGPAW QKIVVCLVFD GIDPCDKDTL DVLATIGVYQ DGVMKRDVDG 310 320 330 340 350 360 KETVAHIFEY TTQLSVTPNQ QLIRPTDDGP STLPPVQMMF CLKQKNSKKI NSHRWLFNAF 370 380 390 400 410 420 GRILNPEVCI LLDAGTKPGP KSLLSLWEAF YNDKDLGGAC GEIHAMLGKG WKNLINPLVA 430 440 450 460 470 480 AQNFEYKISN ILDKPLESSF GYVSVLPGAF SAYRFRAIMG RPLEQYFHGD HTLSKQLGKK 490 500 510 520 530 540 GIEGMNIFKK NMFLAEDRIL CFELVAKAGS KWHLTYVKAS KAETDVPEGA PEFISQRRRW 550 560 570 580 590 600 LNGSFAAGIY SLMHFGRMYK SGHNIVRMFF LHIQMLYNIF STVLTWFSLA SYWLTTTVIM 610 620 630 640 650 660 DLVGTPSDNN GNKAFPFGKT ATPIINTIVK YVYLGFLLLQ FILALGNRPK GSKFSYLASF 670 680 690 700 710 720 VVFGIIQVYV VIDALYLVVR AFSGSAPMDF TTDQGVGEFL KSFFSSSGAG IIIIALAATF 730 740 750 760 770 780 GLYFVASFMY LDPWHMFTSF PAYMCVQSSY INILNVYAFS NWHDVSWGTK GSDKADALPS 790 800 810 820 830 840 AKTTKDEGKE VVIEEIDKPQ ADIDSQFEAT VKRALTPYVP PVEKEEKTLE DSYKSFRTRL 850 860 870 880 890 900 VTFWIFSNAF LAVCITSDGV DKFGFTNSAT DRTQRFFQAL LWSNAVVALF RFIGACWFLG 910 KTGLMCCFAR R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Aspergillus fumigatus chsC and chsG genes encode class III chitin synthases with different functions." Mellado E., Aufauvre-Brown A., Gow N.A.R., Holden D.W. Mol. Microbiol. 20:667-679(1996) [PubMed: 8736545] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: H237.
[2]	"The chsB gene of Aspergillus nidulans is necessary for normal hyphal growth and development." Borgia P.T., Iartchouk N., Riggle P.J., Winter K.R., Koltin Y., Bulawa C.E. Fungal Genet. Biol. 20:193-203(1996) [PubMed: 8953267] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION. Strain: SIU001.
[3]	"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus." Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W. Nature 438:1151-1156(2005) [PubMed: 16372009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Af293 / CBS 101355 / FGSC A1100.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X94244 Genomic DNA. Translation: CAA63928.1. U39478 Genomic DNA. Translation: AAB07678.1. U39479 mRNA. Translation: AAB07679.1. AAHF01000002 Genomic DNA. Translation: EAL92146.1.
PIR	JC6016.
RefSeq	XP_754184.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P54267.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Genome annotation databases
GeneID	3511996.
GenomeReviews	Gene locus chsG in contig CM000171_GR.
KEGG	afm:AFUA_3G14420.
Phylogenomic databases
HOGENOM	P54267.
OMA	YKSGHNI.
Enzyme and pathway databases
BRENDA	2.4.1.16. 18841.
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR013616. Chitin_synth_N. [Graphical view]
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CHSG_ASPFU

Accession

Primary (citable) accession number: P54267
Secondary accession number(s): Q09031, Q09032, Q4WYV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	July 15, 1999
Last modified:	November 3, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)