ID   NIA1_SOYBN              Reviewed;         886 AA.
AC   P54233;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   16-JUN-2009, entry version 68.
DE   RecName: Full=Inducible nitrate reductase [NADH] 1;
DE            Short=NR;
DE            EC=1.7.1.1;
GN   Name=INR1;
OS   Glycine max (Soybean).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Fabales; Fabaceae; Papilionoideae; Phaseoleae;
OC   Glycine.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Williams; TISSUE=Leaf;
RX   MEDLINE=96123229; PubMed=8534848; DOI=10.1007/BF00020980;
RA   Wu S., Lu Q., Kriz A.L., Harper J.E.;
RT   "Identification of cDNA clones corresponding to two inducible nitrate
RT   reductase genes in soybean: analysis in wild-type and nr1 mutant.";
RL   Plant Mol. Biol. 29:491-506(1995).
CC   -!- FUNCTION: Nitrate reductase is a key enzyme involved in the first
CC       step of nitrate assimilation in plants, fungi and bacteria.
CC   -!- CATALYTIC ACTIVITY: Nitrite + NAD(+) + H(2)O = nitrate + NADH.
CC   -!- COFACTOR: Binds 1 FAD.
CC   -!- COFACTOR: Binds 1 heme group. The heme group is called cytochrome
CC       b-557.
CC   -!- COFACTOR: Binds 1 molybdenum ion.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the nitrate reductase family.
CC   -!- SIMILARITY: Contains 1 cytochrome b5 heme-binding domain.
CC   -!- SIMILARITY: Contains 1 FAD-binding FR-type domain.
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DR   EMBL; L23854; AAA96727.1; -; mRNA.
DR   PIR; A59223; A59223.
DR   UniGene; Gma.1221; -.
DR   HSSP; P17571; 2CND.
DR   SMR; P54233; 632-886.
DR   BRENDA; 1.7.1.1; 299.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0050660; F:FAD binding; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0030151; F:molybdenum ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009703; F:nitrate reductase (NADH) activity; IEA:EC.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001199; Cyt_B5.
DR   InterPro; IPR018506; Cyt_B5_heme-BS.
DR   InterPro; IPR017927; Fd_Rdtase_FAD-bd.
DR   InterPro; IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
DR   InterPro; IPR005066; MoCF_OxRdtse_dimer.
DR   InterPro; IPR008335; Mopterin_OxRdtase_euk.
DR   InterPro; IPR001834; NADH-Cyt_B5_reductase.
DR   InterPro; IPR012137; Nitr_rd_NADH.
DR   InterPro; IPR008333; OxRdtase_FAD-bd.
DR   InterPro; IPR001433; OxRdtase_FAD/NAD_bd.
DR   InterPro; IPR000572; OxRdtase_Mopterin-bd.
DR   Gene3D; G3DSA:3.10.120.10; Cyt_B5; 1.
DR   Gene3D; G3DSA:2.60.40.650; MoCF_oxrdtse_dimer; 1.
DR   Gene3D; G3DSA:3.90.420.10; Oxred_molyb_bd; 1.
DR   Pfam; PF00173; Cyt-b5; 1.
DR   Pfam; PF00970; FAD_binding_6; 1.
DR   Pfam; PF03404; Mo-co_dimer; 1.
DR   Pfam; PF00175; NAD_binding_1; 1.
DR   Pfam; PF00174; Oxidored_molyb; 1.
DR   PIRSF; PIRSF000233; Nitr_rd_NADH; 1.
DR   PRINTS; PR00406; CYTB5RDTASE.
DR   PRINTS; PR00363; CYTOCHROMEB5.
DR   PRINTS; PR00407; EUMOPTERIN.
DR   PRINTS; PR00371; FPNCR.
DR   ProDom; PD000612; Cyt_B5; 1.
DR   PROSITE; PS00191; CYTOCHROME_B5_1; 1.
DR   PROSITE; PS50255; CYTOCHROME_B5_2; 1.
DR   PROSITE; PS51384; FAD_FR; 1.
DR   PROSITE; PS00559; MOLYBDOPTERIN_EUK; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; FAD; Flavoprotein; Heme; Iron; Metal-binding;
KW   Molybdenum; NAD; Nitrate assimilation; Oxidoreductase.
FT   CHAIN         1    886       Inducible nitrate reductase [NADH] 1.
FT                                /FTId=PRO_0000166069.
FT   DOMAIN      513    588       Cytochrome b5 heme-binding.
FT   DOMAIN      630    742       FAD-binding FR-type.
FT   METAL       165    165       Molybdenum-pterin (Potential).
FT   METAL       219    219       Molybdenum-pterin (Potential).
FT   METAL       548    548       Iron (heme axial ligand) (By similarity).
FT   METAL       571    571       Iron (heme axial ligand) (By similarity).
FT   DISULFID    404    404       Interchain (Potential).
SQ   SEQUENCE   886 AA;  99799 MW;  C18C73BDE3DE0614 CRC64;
     MAASVDNRQY GTHINAVVRA CGPDFNTPLP SDFDLDSSSD DEDQNDDASF LKELIQKANA
     ETEASLLDPR DEGTADQWIP RNASMVRFTG KHPFNGEGPL PRLMHHGFIT PSPLRYVRNH
     GPVPKIKWDE WTVEVTGLVK RSTHFTMEKL MREFPHREFP ATLVCAGNRR KEHNMVKQSI
     GFNWGAAGGS TSVWRGVPLR HVLKRCGILA RMKGAMYVSF EGAEDLPGGG GSKYGTSVKR
     EMAMDPSRDI ILAFMQNGEP LAPDHGFPVR MIIPGFIGGR MVKWLKRIVV TEHECDSHYH
     YKDNRVLPSH VDAELANDEG WWYKPEYIIN ELNINSVITT PCHEEILPIN SWTTQMPYFI
     RGYAYSGGGR KVTRVEVTLD GGGTWQVCTL DCPEKPNKYG KYWCWCFWSV EVEVLDLLGA
     REIAVRAWDE ALNTQPEKLI WNVMGMMNNC WFRVKTNVCR PHKGEIGIVF EHPTQPGNQS
     GGWMAKEKHL EKSSESNPTL KKSVSSPFMN TTSKTYTMSE VRRHNNADSA WIIVHGHVYD
     WTRFLKDHPG GTDRILINAG TDCTEEFEAI HSDKAKQMLE DYRIGELTTT CYNSDSSSSN
     PSVHGRSDTI PLTPIKEVIT PMRSVALIPR EKIPCKLISK TSISHDVRLF RFGLPSDGLL
     MGLAVGKHIF LCVTVDEKLC MRAYTPTSSV HEVGYFDLVV KVYFKGVHPK FPNGGIMSQH
     LDSLPIGSVL DVKGPLGHIE YTGRGNFLVH GKPRFATRLA MLAGGTGITP IYQVVQAILK
     DPEDCTEMHV VYANRTEDDI LLKEELDEWA KKYDRLKVWY VIQESIREGW EYSVGFITES
     ILTEHIPNAS PDTLALTCGP PPMIQFAVQP NLEKLGYDTQ NNLLVF
//