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Protein

Inducible nitrate reductase [NADH] 1

Gene

INR1

Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + NAD+ + H2O = nitrate + NADH.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • hemeBy similarityNote: Binds 1 heme group. The heme group is called cytochrome b-557.By similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi165MolybdenumBy similarity1
Metal bindingi548Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi571Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrate assimilation

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Inducible nitrate reductase [NADH] 1 (EC:1.7.1.1)
Short name:
NR
Gene namesi
Name:INR1
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
Proteomesi
  • UP000008827 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660691 – 886Inducible nitrate reductase [NADH] 1Add BLAST886

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi404InterchainSequence analysis

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP54233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini513 – 588Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST76
Domaini630 – 742FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Belongs to the nitrate reductase family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiP54233.
KOiK10534.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54233-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASVDNRQY GTHINAVVRA CGPDFNTPLP SDFDLDSSSD DEDQNDDASF
60 70 80 90 100
LKELIQKANA ETEASLLDPR DEGTADQWIP RNASMVRFTG KHPFNGEGPL
110 120 130 140 150
PRLMHHGFIT PSPLRYVRNH GPVPKIKWDE WTVEVTGLVK RSTHFTMEKL
160 170 180 190 200
MREFPHREFP ATLVCAGNRR KEHNMVKQSI GFNWGAAGGS TSVWRGVPLR
210 220 230 240 250
HVLKRCGILA RMKGAMYVSF EGAEDLPGGG GSKYGTSVKR EMAMDPSRDI
260 270 280 290 300
ILAFMQNGEP LAPDHGFPVR MIIPGFIGGR MVKWLKRIVV TEHECDSHYH
310 320 330 340 350
YKDNRVLPSH VDAELANDEG WWYKPEYIIN ELNINSVITT PCHEEILPIN
360 370 380 390 400
SWTTQMPYFI RGYAYSGGGR KVTRVEVTLD GGGTWQVCTL DCPEKPNKYG
410 420 430 440 450
KYWCWCFWSV EVEVLDLLGA REIAVRAWDE ALNTQPEKLI WNVMGMMNNC
460 470 480 490 500
WFRVKTNVCR PHKGEIGIVF EHPTQPGNQS GGWMAKEKHL EKSSESNPTL
510 520 530 540 550
KKSVSSPFMN TTSKTYTMSE VRRHNNADSA WIIVHGHVYD WTRFLKDHPG
560 570 580 590 600
GTDRILINAG TDCTEEFEAI HSDKAKQMLE DYRIGELTTT CYNSDSSSSN
610 620 630 640 650
PSVHGRSDTI PLTPIKEVIT PMRSVALIPR EKIPCKLISK TSISHDVRLF
660 670 680 690 700
RFGLPSDGLL MGLAVGKHIF LCVTVDEKLC MRAYTPTSSV HEVGYFDLVV
710 720 730 740 750
KVYFKGVHPK FPNGGIMSQH LDSLPIGSVL DVKGPLGHIE YTGRGNFLVH
760 770 780 790 800
GKPRFATRLA MLAGGTGITP IYQVVQAILK DPEDCTEMHV VYANRTEDDI
810 820 830 840 850
LLKEELDEWA KKYDRLKVWY VIQESIREGW EYSVGFITES ILTEHIPNAS
860 870 880
PDTLALTCGP PPMIQFAVQP NLEKLGYDTQ NNLLVF
Length:886
Mass (Da):99,799
Last modified:October 1, 1996 - v1
Checksum:iC18C73BDE3DE0614
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23854 mRNA. Translation: AAA96727.1.
PIRiA59223.
RefSeqiNP_001238090.1. NM_001251161.1.
UniGeneiGma.1221.
Gma.8416.

Genome annotation databases

GeneIDi732630.
KEGGigmx:732630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23854 mRNA. Translation: AAA96727.1.
PIRiA59223.
RefSeqiNP_001238090.1. NM_001251161.1.
UniGeneiGma.1221.
Gma.8416.

3D structure databases

ProteinModelPortaliP54233.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi732630.
KEGGigmx:732630.

Phylogenomic databases

InParanoidiP54233.
KOiK10534.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIA1_SOYBN
AccessioniPrimary (citable) accession number: P54233
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 5, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.