Reviewed, UniProtKB/Swiss-Prot P54233 (NIA1_SOYBN)

Names and origin

Protein names

Recommended name:
    Inducible nitrate reductase [NADH] 1
      Short name=NR
    EC=1.7.1.1

Gene names

Name:

INR1

Organism

Glycine max (Soybean)

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	886 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activity	Nitrite + NAD(+) + H(2)O = nitrate + NADH.
Cofactor	Binds 1 FAD. Binds 1 heme group. The heme group is called cytochrome b-557. Binds 1 molybdenum ion.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the nitrate reductase family. Contains 1 cytochrome b5 heme-binding domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	FAD Flavoprotein Heme Iron Metal-binding Molybdenum NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 886	886	Inducible nitrate reductase [NADH] 1		PRO_0000166069
Regions
	Domain	513 – 588	76	Cytochrome b5 heme-binding
	Domain	630 – 742	113	FAD-binding FR-type
Sites
	Metal binding	165	1	Molybdenum-pterin Potential
	Metal binding	219	1	Molybdenum-pterin Potential
	Metal binding	548	1	Iron (heme axial ligand) By similarity
	Metal binding	571	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Disulfide bond	404		Interchain Potential

Sequences

	Sequence		Length	Mass (Da)
	P54233-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: C18C73BDE3DE0614	FASTA	886	99,799
	10 20 30 40 50 60 MAASVDNRQY GTHINAVVRA CGPDFNTPLP SDFDLDSSSD DEDQNDDASF LKELIQKANA 70 80 90 100 110 120 ETEASLLDPR DEGTADQWIP RNASMVRFTG KHPFNGEGPL PRLMHHGFIT PSPLRYVRNH 130 140 150 160 170 180 GPVPKIKWDE WTVEVTGLVK RSTHFTMEKL MREFPHREFP ATLVCAGNRR KEHNMVKQSI 190 200 210 220 230 240 GFNWGAAGGS TSVWRGVPLR HVLKRCGILA RMKGAMYVSF EGAEDLPGGG GSKYGTSVKR 250 260 270 280 290 300 EMAMDPSRDI ILAFMQNGEP LAPDHGFPVR MIIPGFIGGR MVKWLKRIVV TEHECDSHYH 310 320 330 340 350 360 YKDNRVLPSH VDAELANDEG WWYKPEYIIN ELNINSVITT PCHEEILPIN SWTTQMPYFI 370 380 390 400 410 420 RGYAYSGGGR KVTRVEVTLD GGGTWQVCTL DCPEKPNKYG KYWCWCFWSV EVEVLDLLGA 430 440 450 460 470 480 REIAVRAWDE ALNTQPEKLI WNVMGMMNNC WFRVKTNVCR PHKGEIGIVF EHPTQPGNQS 490 500 510 520 530 540 GGWMAKEKHL EKSSESNPTL KKSVSSPFMN TTSKTYTMSE VRRHNNADSA WIIVHGHVYD 550 560 570 580 590 600 WTRFLKDHPG GTDRILINAG TDCTEEFEAI HSDKAKQMLE DYRIGELTTT CYNSDSSSSN 610 620 630 640 650 660 PSVHGRSDTI PLTPIKEVIT PMRSVALIPR EKIPCKLISK TSISHDVRLF RFGLPSDGLL 670 680 690 700 710 720 MGLAVGKHIF LCVTVDEKLC MRAYTPTSSV HEVGYFDLVV KVYFKGVHPK FPNGGIMSQH 730 740 750 760 770 780 LDSLPIGSVL DVKGPLGHIE YTGRGNFLVH GKPRFATRLA MLAGGTGITP IYQVVQAILK 790 800 810 820 830 840 DPEDCTEMHV VYANRTEDDI LLKEELDEWA KKYDRLKVWY VIQESIREGW EYSVGFITES 850 860 870 880 ILTEHIPNAS PDTLALTCGP PPMIQFAVQP NLEKLGYDTQ NNLLVF

References

[1]

"Identification of cDNA clones corresponding to two inducible nitrate reductase genes in soybean: analysis in wild-type and nr1 mutant." Wu S., Lu Q., Kriz A.L., Harper J.E. Plant Mol. Biol. 29:491-506(1995) [PubMed: 8534848] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Williams. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L23854 mRNA. Translation: AAA96727.1.
PIR	A59223.
UniGene	Gma.1221
3D structure databases
HSSP	HSSP built from PDB template 2CND based on UniProtKB P17571.
SMR	P54233. Positions 632-886.
ModBase	Search...
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR001834. Cyt_B5_reductase. IPR001709. FPN_cyt_redctse. IPR005066. MoCF_OxRdtse_dimer. IPR008335. Mopterin_OxRdtase_euk. IPR012137. Nitr_rd_NADH. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. IPR000572. OxRdtase_Mopterin-bd. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit. G3DSA:2.60.40.650. MoCF_oxrdtse_dimer. 1 hit. G3DSA:3.90.420.10. Oxred_molyb_bd. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. PF00970. FAD_binding_6. 1 hit. PF03404. Mo-co_dimer. 1 hit. PF00175. NAD_binding_1. 1 hit. PF00174. Oxidored_molyb. 1 hit. [Graphical view]
PIRSF	PIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTS	PR00406. CYTB5RDTASE. PR00363. CYTOCHROMEB5. PR00407. EUMOPTERIN. PR00371. FPNCR.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. PS51384. FAD_FR. 1 hit. PS00559. MOLYBDOPTERIN_EUK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIA1_SOYBN

Accession

Primary (citable) accession number: P54233

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)